Abstract:
:Lithium Chloride (LiCl) has been used as a canonical Wnt pathway activator due to its ability to inhibit a glycogen synthase kinase-3. The aim of the present study was to investigate the effect of LiCl on cell proliferation and osteogenic differentiation in stem cells isolated from human exfoliated deciduous teeth (SHEDs). SHEDs were isolated and cultured in media supplemented with LiCl at 5, 10, or 20mM. The results demonstrated that LiCl significantly decreased SHEDs colony forming unit ability in a dose dependent manner. LiCl significantly enhanced the percentage of cells in the sub G0 phase, accompanied by a reduction of the percentage of cells in the G1 phase at day 3 and 7 after treatment. Further, LiCl markedly decreased OSX and DMP1 mRNA expression after treating SHEDs in an osteogenic induction medium for 7 days. In addition, no significant difference in alkaline phosphatase enzymatic activity or mineral deposition was found. Together, these results imply that LiCl influences SHEDs behavior.
journal_name
Tissue Celljournal_title
Tissue & cellauthors
Rattanawarawipa P,Pavasant P,Osathanon T,Sukarawan Wdoi
10.1016/j.tice.2016.08.005subject
Has Abstractpub_date
2016-10-01 00:00:00pages
425-31issue
5eissn
0040-8166issn
1532-3072pii
S0040-8166(16)30129-Xjournal_volume
48pub_type
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