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The structure and function of the smooth septate junction in a transporting epithelium: the Malpighian tubules of the New Zealand glow-worm Arachnocampa luminosa.

Abstract：

:Junctional complexes between the epithelial cells in the four distinct regions of the glow-worm Malpighian tubule were investigated by electron microscopy using thin sectioning, freeze-fracturing, osmotic disruption and tracer techniques. The lateral plasma membranes of all four cell types are joined by smooth septate junctions but the extent of the complex across the cell depth varies in the four different regions. The width of the septa, the interseptal spacing and the separation between the outer leaflets of the adjacent plasma membranes are different for each cell type. Gap junctions were identified only in the junctional complex between Type IV cells and were intercalated amongst large lateral sinuses. In oblique sections of lanthanum infiltrated tissue, the electron-lucent septa at the basal side of the junction are outlined by the tracer as it penetrates. In the junctional complexes of all four regions the septa appear as short, distinct, linear bars. In tangential sections of gap junctions between Type IV cells, the junctions appear as a hexagonal array of intermembrane particles with a centre to centre spacing of 18 nm. Horseradish peroxidase did not penetrate the junctional complexes very far but readily passed through the basal lamina into the spaces between extracellular invaginations of the basement membrane of the cells. Junctional complexes in all four areas of the tubule have similar freeze-fracture faces. In freeze-fracture replicas of fixed tissue continuous ridges of fused particles are seen on the P face and complementary furrows are found on the E face. Junctional response to osmotically adjusted Ringer solutions was similar in all four cell types. Distortion or 'blistering' of the intercellular space between between the septa of the unction occurred when the tissue was bathed in or injected with a hypertonic Ringer solution. The structure of these junctions, visualized by the different techniques, and the role of the septate junction in a transporting epithelium, are discussed.

journal_name

Tissue Cell

journal_title

Tissue & cell

authors

Green LF,Bergquist PR,Bullivant S

doi

10.1016/0040-8166(80)90011-7

subject

Has Abstract

pub_date

1980-01-01 00:00:00

pages

365-81

issue

eissn

0040-8166

issn

1532-3072

pii

0040-8166(80)90011-7

journal_volume

pub_type

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The structure and function of the smooth septate junction in a transporting epithelium: the Malpighian tubules of the New Zealand glow-worm Arachnocampa luminosa.