Differential distribution of glutamylated tubulin in the flagellum of mouse spermatozoa.

Abstract:

:Using a monoclonal antibody (GT 335) we previously demonstrated that glutamylation is a predominant posttranslational modification of alpha and beta tubulin isoforms in the axoneme of mouse spermatozoa (Fouquet et al., Cell Motil. Cytoskel. 27, 49, 1994). However, we noted that the staining intensity and/or distribution of glutamylated tubulin were not identical using either indirect immunofluorescence (IIF) or immunoelectron microscopy. To test this discrepancy various permeabilization procedures were performed for IIF: methanol or acetone alone or in combination, including freezing pretreatment and with or without paraformaldehyde fixation. Each procedure gave a particular labeling of sperm axoneme. The diversity of axoneme labeling in mouse spermatids and spermatozoa appeared dependent both on the absence or presence of periaxonemal sheaths and permeabilization procedures. For comparison with IIF and to avoid problematic premeabilization treatments a quantitative postembedding immunogold approach was preferred. In these conditions the labeling predominated in the middle piece of the sperm flagellum and decreased progressively in the principal piece. However, the labeling of the terminal piece was similar to that of the middle piece. These results suggested a differential glutamylated tubulin distribution along the axoneme of the mouse sperm flagellum.

journal_name

Tissue Cell

journal_title

Tissue & cell

authors

Kann ML,Prigent Y,Fouquet JP

doi

10.1016/s0040-8166(95)80053-0

subject

Has Abstract

pub_date

1995-06-01 00:00:00

pages

323-9

issue

3

eissn

0040-8166

issn

1532-3072

pii

S0040-8166(95)80053-0

journal_volume

27

pub_type

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