Differential distribution of glutamylated tubulin in the flagellum of mouse spermatozoa.


:Using a monoclonal antibody (GT 335) we previously demonstrated that glutamylation is a predominant posttranslational modification of alpha and beta tubulin isoforms in the axoneme of mouse spermatozoa (Fouquet et al., Cell Motil. Cytoskel. 27, 49, 1994). However, we noted that the staining intensity and/or distribution of glutamylated tubulin were not identical using either indirect immunofluorescence (IIF) or immunoelectron microscopy. To test this discrepancy various permeabilization procedures were performed for IIF: methanol or acetone alone or in combination, including freezing pretreatment and with or without paraformaldehyde fixation. Each procedure gave a particular labeling of sperm axoneme. The diversity of axoneme labeling in mouse spermatids and spermatozoa appeared dependent both on the absence or presence of periaxonemal sheaths and permeabilization procedures. For comparison with IIF and to avoid problematic premeabilization treatments a quantitative postembedding immunogold approach was preferred. In these conditions the labeling predominated in the middle piece of the sperm flagellum and decreased progressively in the principal piece. However, the labeling of the terminal piece was similar to that of the middle piece. These results suggested a differential glutamylated tubulin distribution along the axoneme of the mouse sperm flagellum.


Tissue Cell


Tissue & cell


Kann ML,Prigent Y,Fouquet JP




Has Abstract


1995-06-01 00:00:00














  • Ultrastructural analysis of progressive endometrial hypercytolipidemia induced by obese (ob/ob) and diabetes (db/db) genotype mutations: structural basis of female reproductive tract involution.

    abstract::The diabetes (db/db) and obese (ob/ob) genotype mutations induce a progressive, hypercytolipidemic condition within the endometrium of the female reproductive tract that promotes sterility and premature organ involution in C57BL/KsJ mice. The current studies focus on the ultrastructural changes that occur within the e...

    journal_title:Tissue & cell

    pub_type: 杂志文章


    authors: Garris DR

    更新日期:2004-02-01 00:00:00

  • Early origins of definitive erythroid cells in the chick embryo.

    abstract::The early maturation stages of definitive erythroid cells are observed in the embryonic circulation of the chick yolk sac at 4.5--5 days of incubation. Light and electron microscope observation of the mesoderm of the yold sac membrane indicate that individual presumptive precursors of the definitive-line are present a...

    journal_title:Tissue & cell

    pub_type: 杂志文章


    authors: Dardick I,Setterfield G

    更新日期:1978-01-01 00:00:00

  • Fine structural organization of the spermatheca in the cockroach, Periplaneta americana.

    abstract::The detailed structure of the cockroach spermatheca is described and discussed firstly as an example of an insect integumentary gland, and secondly, from the standpoint of its role in reproduction. The gland comprises a cortical rank of separate secretory units, each associated with an epithelial duct cell responsible...

    journal_title:Tissue & cell

    pub_type: 杂志文章


    authors: Gupta BL,Smith DS

    更新日期:1969-01-01 00:00:00

  • Ultrastructure of peritubular tissue in association with tubular hyalinization in human testis.

    abstract::Testicular peritubular tissue, also known as the tunica propria, surrounds the seminiferous tubules and is responsible for contractile, paracrine and transport functions. The aim of the present report is to describe the pathomorphology of peritubular tissue in association with tubular hyalinization in human testis. Tw...

    journal_title:Tissue & cell

    pub_type: 临床试验,杂志文章


    authors: Haider SG,Talati J,Servos G

    更新日期:1999-02-01 00:00:00

  • Down-regulation of SOX17, GATA4 and FoxA2 promotes differentiation potential of hepatocytes from human hematopoietic stem cells.

    abstract::The failure of regeneration of damaged liver in various end-stage liver diseases results in high morbidity and mortality. In this context, we have demonstrated the differentiation ability of human hematopoietic stem cells (HSCs) into hepatocytes. In this study, HSCs were isolated from a donor and cultured which exhibi...

    journal_title:Tissue & cell

    pub_type: 杂志文章


    authors: Kumar PS,Chandrasekhar C,Srikanth L,Sarma PVGK

    更新日期:2020-02-01 00:00:00

  • Tyrosine storage vacuoles in insect fat body.

    abstract::The watery vacuoles first described from larval insect fat body (Chironomus, Voinov, 1927; Aedes, Wigglesworth, 1942; Rhodnius, Wigglesworth, 1967) have been studied in 4th and 5th stage Calpodes larvae. The vacuoles arise at the beginning (E + 6-24 hr) of the 4th stadium from plasma membrane infolds that separate fro...

    journal_title:Tissue & cell

    pub_type: 杂志文章


    authors: McDermid H,Locke M

    更新日期:1983-01-01 00:00:00

  • Swelling of the collagen-keratocyte matrix of the bovine corneal stroma ex vivo in various solutions and its relationship to tissue thickness.

    abstract:AIM:The mammalian corneal stroma, like some other connective tissues, can absorb fluid, swell and become oedematous. Since studies on the corneal stroma have been carried out with different types of preparations and solutions, inter-study comparisons are very difficult. A study was thus undertaken on a standardised pre...

    journal_title:Tissue & cell

    pub_type: 杂志文章


    authors: Doughty MJ

    更新日期:2000-12-01 00:00:00

  • Macrophage lipid body induction by Chagas disease in vivo: putative intracellular domains for eicosanoid formation during infection.

    abstract::Lipid bodies (LB), lipid-rich inclusions abundantly present in cells engaged in inflammation, are specialized intracellular domains involved in generating inflammatory mediators, the eicosanoids. Since the acute Trypanosoma cruzi infection triggers a potent inflammatory reaction characterized by a great increase of pe...

    journal_title:Tissue & cell

    pub_type: 杂志文章


    authors: Melo RC,D'Avila H,Fabrino DL,Almeida PE,Bozza PT

    更新日期:2003-02-01 00:00:00

  • Modulation of mThy28 nuclear protein expression during thymocyte development.

    abstract::We have recently shown that down-regulation of mouse Thy28 (mThy28) protein expression appears to be accompanied by apoptotic processes. Thymocytes from mice contain moderate amounts of mThy28 protein and undergo proliferation, differentiation, or apoptosis during murine thymic maturation. As a first step to examine t...

    journal_title:Tissue & cell

    pub_type: 杂志文章


    authors: Jiang X,Toyota H,Takada E,Yoshimoto T,Kitamura T,Yamada J,Mizuguchi J

    更新日期:2003-12-01 00:00:00

  • Effects of Toll-like receptor ligands on RAW 264.7 macrophage morphology and zymosan phagocytosis.

    abstract::In this study we compared the effects of the Toll-like receptor (TLR) ligands lipopolysaccharide (LPS), flagellin, the synthetic bacterial triacylated lipopeptide Pam3-Cys-Ser-Lys4 (Pam3CSK4), Polyinosinic:polycytidylic acid (Poly I:C), and macrophage-activating lipopeptide (MALP-2), which are TLR4, TLR5, TLR1/2, TLR3...

    journal_title:Tissue & cell

    pub_type: 杂志文章


    authors: Sigola LB,Fuentes AL,Millis LM,Vapenik J,Murira A

    更新日期:2016-08-01 00:00:00

  • Growth factors and chondrogenic differentiation of mesenchymal stem cells.

    abstract::The main purpose of the article is to review recent knowledge about growth factors and their effect on the chondrogenic differentiation of mesenchymal stem cells under in vitro conditions. Damaged or lost articular cartilage leads to progressive debilitation, which have major impact on the life quality of the affected...

    journal_title:Tissue & cell

    pub_type: 杂志文章,评审


    authors: Danišovič L,Varga I,Polák S

    更新日期:2012-04-01 00:00:00

  • Cleaning insect oocytes by dissection and enzyme treatment.

    abstract::Vitellogenic oocytes from the cockroach Blattella germanica were dissected free of the surrounding follicle cell layer. The continued presence of a resting potential indicated that the defolliculated oocytes remained viable. In other experiments, lysozyme was injected into last nymphal instars; scanning electron micro...

    journal_title:Tissue & cell

    pub_type: 杂志文章


    authors: Anderson M,Kunkel JG

    更新日期:1990-01-01 00:00:00

  • Automated oral squamous cell carcinoma identification using shape, texture and color features of whole image strips.

    abstract::Despite profound knowledge of the incidence of oral cancers and a large body of research beyond it, it continues to beat diagnosis and treatment management. Post physical observation by clinicians, a biopsy is a gold standard for accurate detection of any abnormalities. Towards the application of artificial intelligen...

    journal_title:Tissue & cell

    pub_type: 杂志文章


    authors: Rahman TY,Mahanta LB,Das AK,Sarma JD

    更新日期:2020-04-01 00:00:00

  • Testicular denervation-induced nuclear changes in Leydig cell of hemicastrated adult rats.

    abstract::In the adult male rat, hemicastration (HC) induces a remarkable enhancement of testosterone secretion from the Leydig cells of the remaining testis. We have observed previously that the denervation of inferior spermatic nerves inhibits this enhancement. The present experiments were designed to assess morphometrically ...

    journal_title:Tissue & cell

    pub_type: 杂志文章


    authors: Zhu B,Cavicchia JC,Chiocchio SR

    更新日期:1998-10-01 00:00:00

  • The sarcoplasmic reticulum of an ultrafast lobster muscle: first evidence of a tubular configuration.

    abstract::The sarcoplasmic reticulum (SR) of the remarkably fast remotor muscle of lobster second antenna was investigated with regard to ultrastructure, calcium uptake and protein composition. The SR of this unique muscle dominates the volume of the cell. We were able to preserve the configuration of the SR in its native state...

    journal_title:Tissue & cell

    pub_type: 杂志文章


    authors: Scales DJ,Kidd P,Yasumura T,Ines G

    更新日期:1982-01-01 00:00:00

  • Histochemical specificity of cholinesterases to phenylthioacetate in differentiated neural tissues of insects and teleosts.

    abstract::Housefly brain cholinesterase was histochemically demonstrated to hydrolyse phenylthioacetate at a very high rate, similar in distribution to that previously reported for acetylthiocholine. However, teleost neural retina cholinesterase would not hydrolyse the aromatic substrate, but the enzyme did cleave acetylthiocho...

    journal_title:Tissue & cell

    pub_type: 杂志文章


    authors: Booth GM,Whitt GS

    更新日期:1970-01-01 00:00:00

  • Partial development of the steroidogenic ultrastructural features in degenerative corpora lutea after a single injection of pituitary extract in the Western painted turtle (Chrysemys picta).

    abstract::Pituitary glands were removed from sexually mature female turtles (Chrysemys picta) and they were injected intraperitoneally (i.p.) into other mature females of the same species (experimental). In addition mature females of the same species received saline injection only (controls). Initially all the turtles used in t...

    journal_title:Tissue & cell

    pub_type: 杂志文章


    authors: Mahmoud IY,Ba-Omar T,Alkindi A

    更新日期:2006-06-01 00:00:00

  • Characterization and spatial relationships of the hepatic vascular-biliary tracts, and their associated pancreocytes and macrophages, in the model fish guppy (Poecilia reticulata): A study of serial sections by light microscopy.

    abstract::The guppy is a tropical fish that has been used as an experimental model organism in science. It is a species well adapted to the natural environment and that can support adverse environmental conditions, and so, at occasions, its presence can be indicative of environmental disturbances. Moreover, as the liver is very...

    journal_title:Tissue & cell

    pub_type: 杂志文章


    authors: Sousa S,Rocha MJ,Rocha E

    更新日期:2018-02-01 00:00:00

  • Arthropod immune system. III. Septate junctions in the hemocytic capsule of the german cockroach, Blattella germanica.

    abstract::Granulocytes (GRs) and/or plasmatocytes (PLs), the two major immunocytes in arthropods, participate in cellular encapsulation of foreign tissue. Although gap and desmosome junctions have been reported in insect capsules, smooth septate junctions are being reported for the first time by both thin section and freeze-fra...

    journal_title:Tissue & cell

    pub_type: 杂志文章


    authors: Gupta AP,Han SS

    更新日期:1988-01-01 00:00:00

  • Gold labelling of RNA in virus-induced mitochondrial vesicles in the sheep blowfly Lucilia cuprina.

    abstract::Membranous vesicles are thought to be the replication site for viral RNA of many plant and animal viruses. A relatively rare site of virus-associated vesiculation is that of the mitochondrial outer membrane. In this study, virus-induced mitochondrial vesicles of the blowfly, Lucilia cuprina, were labelled with ribonuc...

    journal_title:Tissue & cell

    pub_type: 杂志文章


    authors: Binnington K,Brooks L

    更新日期:1992-01-01 00:00:00

  • Finite element simulation of an artificial intervertebral disk using fiber reinforced laminated composite model.

    abstract::Degeneration of intervertebral disk (IVD) has been increased in recent years. The lumbar herniation can be cured using conservative and surgical procedures. Surgery is considered after failure of conservative treatment. Partial discectomy, fusion, and total disk replacement (TDR) are also common surgical treatments fo...

    journal_title:Tissue & cell

    pub_type: 杂志文章


    authors: Shahmohammadi M,Asgharzadeh Shirazi H,Karimi A,Navidbakhsh M

    更新日期:2014-10-01 00:00:00

  • Ultrastructure of the putative stem cell niche in rat mammary epithelium.

    abstract::There is now strong evidence that the stem cells of many tissues reside in specialized structures termed niches. The stem cell niche functions to house and regulate symmetric and asymmetric mitosis of stem cells in mammalian skin, mouse and human bone marrow, mouse brain, gut, and hair follicle, and Drosophila ovary a...

    journal_title:Tissue & cell

    pub_type: 杂志文章


    authors: Chepko G,Dickson RB

    更新日期:2003-04-01 00:00:00

  • The tight junctions of rabbit choroid plexus and ciliary body epithelia. A comparative study using the double replica freeze-fracture technique.

    abstract::The spatial arrangement of tight junctions in choroid plexus and ciliary body rabbit epithelia has been determined by studying freeze-fracture complementary replicas. In the choroid plexus epithelium, the interruptions of the junctional P-face fibrils were measured to be 14% in their total length. In the ciliary body ...

    journal_title:Tissue & cell

    pub_type: 杂志文章


    authors: Hirsch M,Verge D,Bouchaud C,Escaig J

    更新日期:1980-01-01 00:00:00

  • Protein profiling and angiogenic effect of hypoxia-cultured human umbilical cord blood-derived mesenchymal stem cells in hindlimb ischemia.

    abstract::The aim of the present study was to investigate protein profiles of human umbilical cord blood-derived mesenchymal stem cells (hUCB-MSCs) cultured in normoxic (21% O2) and hypoxic (1% O2) conditions, and evaluate oxygenation effects on angiogenesis in an ischemic hindlimb mouse model using a modified ischemic scoring ...

    journal_title:Tissue & cell

    pub_type: 杂志文章


    authors: Han KH,Kim AK,Kim MH,Kim DH,Go HN,Kang D,Chang JW,Choi SW,Kang KS,Kim DI

    更新日期:2017-12-01 00:00:00

  • The effects of castration followed testosterone supplementation in prostatic complex of Artibeus planirostris (Chiroptera: Phyllostomidae).

    abstract::The prostatic complex (ventral and dorsal regions) of Artibeus planirostris exhibits seasonal variations throughout the year. Circulating testosterone was correlated with prostate weight, showing an increase from autumn to summer, with the highest peak in summer corresponding to the largest breeding season. This indic...

    journal_title:Tissue & cell

    pub_type: 杂志文章


    authors: Puga CC,Beguelini MR,Morielle-Versute E,Vilamaior PS,Taboga SR

    更新日期:2016-06-01 00:00:00

  • Na(+)/K(+)-ATPase activity and immunocytochemical labeling in podobranchial filament and lamina of the freshwater crayfish Astacus leptodactylus Eschscholtz: evidence for the existence of sodium transport in the filaments.

    abstract::In the crayfish Astacus leptodactylus, the podobranch, bearing respiratory and ion-transporting filaments, is attached to the lamina. The Na(+)/K(+)-ATPase activity is higher in filaments than in lamina. Using a mouse monoclonal antibody directed against the alpha subunit of Na(+)/K(+)-ATPase, and indirect immunofluor...

    journal_title:Tissue & cell

    pub_type: 杂志文章


    authors: Barradas C,Wilson JM,Dunel-Erb S

    更新日期:1999-10-01 00:00:00

  • Ultrastructure studies of the extracellular filaments in the ventral nerve of Panulirus argus.

    abstract::The ventral nerve cord of the spiny lobster, Panulirus argus was examined by transmission and scanning electron microscopy. Tannic acid mordant stain was used to enhance extracellular filaments. The ventral nerve cord is surrounded by an unusual perineurial sheath composed primarily of interwoven extracellular filamen...

    journal_title:Tissue & cell

    pub_type: 杂志文章


    authors: Williamson LC,Baerwald RJ

    更新日期:1990-01-01 00:00:00

  • Role of epidermal stem cells in repair of partial-thickness burn injury after using Moist Exposed Burn Ointment (MEBO(®)) histological and immunohistochemical study.

    abstract::Moist Exposed Burn Ointment (MEBO(®)) is widely used topical agent applied on skin burn. This study investigated the effect of MEBO topical application on activation and proliferation of epidermal stem cells through the immunohistochemical localization of cytokeratin 19 (CK19) as a known marker expressed in epidermal ...

    journal_title:Tissue & cell

    pub_type: 杂志文章


    authors: El-Hadidy MR,El-Hadidy AR,Bhaa A,Asker SA,Mazroa SA

    更新日期:2014-04-01 00:00:00

  • 'Twisted plywood' structure and mineralization in the scales of a primitive living fish Amia calva.

    abstract::The basal plate of the scales of Amia calva is composed of regular double twisted plywood, as in Latimeria and Dipnoan scales. However, the progressive rotation of the fibrils direction is left-handed in Amia and right-handed in the 'Sarcopterygians'. So, the similarity between these peculiar plywoods is probably the ...

    journal_title:Tissue & cell

    pub_type: 杂志文章


    authors: Meunier FJ

    更新日期:1981-01-01 00:00:00

  • Sites of lanthanum occlusion in the testis of the crayfish Procambarus paeninsulanus (Crustacea: Cambaridae).

    abstract::The presence of stage-dependent occlusive junctions between adjacent Sertoli cells in the seminiferous epithelium of the crayfish testis was demonstrated by a lanthanum tracer study. The germinal epithelium did not appear to be compartmentalized, as evidenced by access of lanthanum to spermatogonia, spermatocytes, and...

    journal_title:Tissue & cell

    pub_type: 杂志文章


    authors: Harvey MC,Hinsch GW,Cameron DF

    更新日期:2001-12-01 00:00:00