Abstract:
:Using a monoclonal antibody (GT 335) we previously demonstrated that glutamylation is a predominant posttranslational modification of alpha and beta tubulin isoforms in the axoneme of mouse spermatozoa (Fouquet et al., Cell Motil. Cytoskel. 27, 49, 1994). However, we noted that the staining intensity and/or distribution of glutamylated tubulin were not identical using either indirect immunofluorescence (IIF) or immunoelectron microscopy. To test this discrepancy various permeabilization procedures were performed for IIF: methanol or acetone alone or in combination, including freezing pretreatment and with or without paraformaldehyde fixation. Each procedure gave a particular labeling of sperm axoneme. The diversity of axoneme labeling in mouse spermatids and spermatozoa appeared dependent both on the absence or presence of periaxonemal sheaths and permeabilization procedures. For comparison with IIF and to avoid problematic premeabilization treatments a quantitative postembedding immunogold approach was preferred. In these conditions the labeling predominated in the middle piece of the sperm flagellum and decreased progressively in the principal piece. However, the labeling of the terminal piece was similar to that of the middle piece. These results suggested a differential glutamylated tubulin distribution along the axoneme of the mouse sperm flagellum.
journal_name
Tissue Celljournal_title
Tissue & cellauthors
Kann ML,Prigent Y,Fouquet JPdoi
10.1016/s0040-8166(95)80053-0subject
Has Abstractpub_date
1995-06-01 00:00:00pages
323-9issue
3eissn
0040-8166issn
1532-3072pii
S0040-8166(95)80053-0journal_volume
27pub_type
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