Abstract：
OBJECTIVE:The current study proposes an automated machine learning approach for the quantification of cells in cell death pathways according to DNA fragmentation. METHODS:A total of 17 images of kidney histological slide samples from male Wistar rats were used. The slides were photographed using an Axio Zeiss Vert.A1 microscope with a 40x objective lens coupled with an Axio Cam MRC Zeiss camera and Zen 2012 software. The images were analyzed using CellProfiler (version 2.1.1) and CellProfiler Analyst open-source software. RESULTS:Out of the 10,378 objects, 4970 (47,9%) were identified as TUNEL positive, and 5408 (52,1%) were identified as TUNEL negative. On average, the sensitivity and specificity values of the machine learning approach were 0.80 and 0.77, respectively. CONCLUSION:Image cytometry provides a quantitative analytical alternative to the more traditional qualitative methods more commonly used in studies.
journal_name
Tissue Celljournal_title
Tissue & cellauthors
Macedo ND,Buzin AR,de Araujo IB,Nogueira BV,de Andrade TU,Endringer DC,Lenz Ddoi
10.1016/j.tice.2016.12.006subject
Has Abstractpub_date
2017-02-01 00:00:00pages
22-27issue
1eissn
0040-8166issn
1532-3072pii
S0040-8166(16)30173-2journal_volume
49pub_type
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