Abstract：
:The dynamics of human and animal adipogenesis has been defined using several traditional cell systems including stromal vascular cells and adipocyte-related cell lines. But a relatively new cell system using progeny cells stemming from the dedifferentiation of purified cultures of mature adipocytes may be used for studying the development and biology of adipocytes. In this research, we show that isolated (and purified) mature adipocytes derived from Wagyu cattle dedifferentiate into progeny cells, and that these spindle-shaped, proliferative-competent daughter cells possess ability to proliferate. We outline the optimum cell culture system and offer precautionary thoughts for effective mature adipocyte culture. Collectively, this represents a novel cell model which may provide new insights into cell development, physiology and use as a model for animal production/composition, tissue engineering and disease treatment.
journal_name
Tissue Celljournal_title
Tissue & cellauthors
Wei S,Duarte MS,Du M,Paulino PV,Jiang Z,Albrecht E,Fernyhough-Culver M,Zan L,Hausman GJ,Dodson MVdoi
10.1016/j.tice.2012.08.001subject
Has Abstractpub_date
2012-12-01 00:00:00pages
385-90issue
6eissn
0040-8166issn
1532-3072pii
S0040-8166(12)00067-5journal_volume
44pub_type
杂志文章相关文献
TISSUE & CELL文献大全abstract：:Autologous fibrin has been widely used in surgical procedures for both soft and hard tissue repair. There are different protocols and devices to obtain this matrix, with varying centrifugal time, gravity force, speed, angle of the sample tube and spinning radius. The aim of this study was to compare three methods of o...
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1016/j.tice.2020.101407
更新日期：2020-12-01 00:00:00
abstract：:Two systems of microtubules appear to be functionally significant in the transformation of a small cell into an elongated, acellular 'corkscrew', which represents the anterior region of a sperm bundle of Pseudococcus obscurus. One system of microtubules lies within the cell; it is oriented parallel to the long axis of...
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1016/s0040-8166(71)80029-0
更新日期：1971-01-01 00:00:00
abstract：:Human amniotic mesenchymal stromal cells (hAMSCs) are considered as a population of multipotent cells. The molecular events associated with mesenchymal stromal cell (MSC)/tumor cell interactions should be studied to identify the role of MSCs on suppressing or inducing the key signaling pathways of tumor cells. Thus, d...
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1016/j.tice.2020.101479
更新日期：2021-02-01 00:00:00
abstract：:The Australian lungfish, Neoceratodus forsteri, has a dentition consisting of enamel, mantle dentine and bone, enclosing circumdenteonal, core and interdenteonal dentines. Branching processes from cells that produce interdenteonal dentine leave the cell surface at different angles, with collagen fibrils aligned parall...
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1016/j.tice.2006.01.001
更新日期：2006-04-01 00:00:00
abstract：:The aim of the present study was to investigate protein profiles of human umbilical cord blood-derived mesenchymal stem cells (hUCB-MSCs) cultured in normoxic (21% O2) and hypoxic (1% O2) conditions, and evaluate oxygenation effects on angiogenesis in an ischemic hindlimb mouse model using a modified ischemic scoring ...
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1016/j.tice.2017.09.006
更新日期：2017-12-01 00:00:00
abstract：:In the seminal vesicle of the 'symphyta'Arge pagana the spermatozoa are stored in motile spermatodesm bundles, maintained by an anterior cap of extracellular material. This cap consists of a denser cortex and of an internal matrix, where part of the sperm heads are embedded. The number of spermatozoa per bundle is var...
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1016/j.tice.2007.11.004
更新日期：2008-06-01 00:00:00
abstract：:Using a monoclonal antibody (GT 335) we previously demonstrated that glutamylation is a predominant posttranslational modification of alpha and beta tubulin isoforms in the axoneme of mouse spermatozoa (Fouquet et al., Cell Motil. Cytoskel. 27, 49, 1994). However, we noted that the staining intensity and/or distributi...
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1016/s0040-8166(95)80053-0
更新日期：1995-06-01 00:00:00
abstract：:Aromatization of androgens into estrogens in rat testis is catalyzed by the microsomal enzyme cytochrome P450 aromatase. In this work, aromatase cellular site was investigated in prepuberal, peripuberal and postpuberal testis, from 10-, 21- and 60-day-old rats respectively. Paraffin-embedded testis sections were proce...
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1054/tice.2001.0186
更新日期：2001-08-01 00:00:00
abstract：:In tendon lesions, inflammation indicates the beginning of tissue repair and influences cell proliferation and the remodeling of the extracellular matrix (ECM). Low level laser (LLL) therapy has been an important method to induce tissue repair, and several studies have sought to better understand the therapeutic possi...
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1016/j.tice.2017.05.006
更新日期：2017-08-01 00:00:00
abstract：:Bovine adrenal medulla capillary endothelial cells were isolated and cloned, and their morphological behaviors in vitro were examined. In the culture of primary or early passage, one type of colony formed intracellular lumina both on the dish and in the three dimensional collagen gel. Another type proliferated well an...
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1016/0040-8166(90)90059-i
更新日期：1990-01-01 00:00:00
abstract：:The loci of calcium distribution in Nereis pharyngeal visceral muscle have been examined by cytochemical precipitation using potassium pyroantimonate. In Na-, Ca- and Mg-free media, pyroantimonate incubation was used to pinpoint loci of intracellularly bound calcium. This method also revealed heavy deposition on the i...
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1016/0040-8166(81)90024-0
更新日期：1981-01-01 00:00:00
abstract：:In this study, we aimed to investigate the presence of asprosin (ASP) in the liver, kidneys, heart, stomach, testicles and brain and to determine the serum and tissue asprosin levels in diabetic rats. A total of 14 male Wistar Albino rats were divided into two groups, each containing 7 rats: (I) control group and (II)...
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1016/j.tice.2020.101397
更新日期：2020-10-01 00:00:00
abstract：:To develop means of measuring angiotensin converting enzyme of endothelial cells in culture, we have synthesized benzoyl-Phe-Ala-Pro-OH (I), benzoyl-Pro-Phe-Arg-OH (II) and benzoyl-Gly-His-Leu-OH (III), each bearing a 3H-atom on the para-position of its benzoyl moiety. All three of the acylated tripeptides are substra...
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1016/s0040-8166(16)30348-2
更新日期：1978-01-01 00:00:00
abstract：:The structure of the sclerite and intersegmental cuticle of the opithosoma of the desert scorpion, Hadrurus arizonensis, has been examined by transmission electron microscopy. The sclerite cuticle contains a four-layered epicuticle, a hyaline exocuticle, an inner exocuticle and an endocuticle. The outer part of the hy...
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1016/0040-8166(79)90040-5
更新日期：1979-01-01 00:00:00
abstract：:The periostracum in Unionidae consists of two layers. The outer one is secreted within the periostracal groove, while the inner layer is secreted by the epithelium of the outer mantle fold. The periostracum reaches its maximum thickness at the shell edge, where it reflects onto the shell surface. Biomineralization beg...
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1054/tice.2000.0129
更新日期：2000-10-01 00:00:00
abstract：:Lactational function in the mammary epithelial cell is subject to complex regulation, most probably involving multiple extracellular and intracellular proteins that act at any of a number of levels. Although some of these proteins have been identified it is likely that additional controllers of lactation exist, but ha...
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1016/s0040-8166(97)80051-1
更新日期：1997-10-01 00:00:00
abstract：:We have reported that telomere fluorescence units (TFUs) of established induced pluripotent stem cells (iPSCs) derived from human amnion (hAM933) and fetal lung fibroblasts (MRC-5) were significantly longer than those of the parental cells, and that the telomere extension rates varied quite significantly among clones ...
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1016/j.tice.2014.08.005
更新日期：2014-12-01 00:00:00
abstract：:Bone substitutes are used in nearly half of all musculoskeletal surgeries. The "gold standard" graft is an autograft that is limited by supply and site morbidity. Therefore, allograft sources are the current alternative for clinical practice with some side effects, such as immune responses and risk of disease transmis...
journal_title：Tissue & cell
pub_type： 杂志文章,评审
doi：10.1016/j.tice.2021.101494
更新日期：2021-01-16 00:00:00
abstract：:Vitellogenic oocytes from the cockroach Blattella germanica were dissected free of the surrounding follicle cell layer. The continued presence of a resting potential indicated that the defolliculated oocytes remained viable. In other experiments, lysozyme was injected into last nymphal instars; scanning electron micro...
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1016/0040-8166(90)90009-x
更新日期：1990-01-01 00:00:00
abstract：:We have previously shown that indapamide [chloro-4-N-(methyl-2-indolinyl-1)-sulfamoyal-3-benzamide] has a direct action on the heart to alter ion fluxes. This study sought to examine the potential interaction between indapamide and epidermal growth factor (EGF). Cardiomyocytes were prepared as primary culture from 7-d...
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1016/s0040-8166(96)80032-2
更新日期：1996-08-01 00:00:00
abstract：:The role of phagocytosis in eliminating apoptotic spermatogenic cells caused by mono(2-ethylhexyl) phthalate (MEHP) was studied. Twenty-one-day-old C57Bl/6N male mice were given a single dose of 800 mg/kg MEHP in corn oil by oral gavage and sacrificed at 1, 3, 5, 7 and 9 days after initial exposure. At the same time, ...
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1016/j.tice.2007.04.002
更新日期：2007-08-01 00:00:00
abstract：:The primary musculature of Sagitta is mainly made up of two kinds of alternating fibers, A and B. These fibers differ markedly in their localization in the muscular tissue, by the development of their SR and their mitochondria, and the shape of their myofibrils. Their contractile apparatus is similar and possesses myo...
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1016/0040-8166(80)90025-7
更新日期：1980-01-01 00:00:00
abstract：:Sporogony of the sporozoan Theileria parva in the salivary gland of the tick vector of East Coast fever was studied in electron micrographs. The findings differ in several respects from previous interpretations based upon light microscopy. Cytokinesis of the primary sporoblast to form secondary and tertiary sporoblast...
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1016/0040-8166(82)90017-9
更新日期：1982-01-01 00:00:00
abstract：:Diabetic nephropathy is the common cause of leading to end stage of renal disease (ESRD). Satureja khozestanica essential oil (SKEO) was used as an antioxidant and antidiabetic for the inhibition of diabetic nephropathy. Forty male rats were uninephrectomized and divided in four groups randomly; group one as control, ...
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1016/j.tice.2010.11.004
更新日期：2011-02-01 00:00:00
abstract：:Until primate sperm are exposed to the unique microenvironment of the epididymis, they are not capable of fertilization or vigorous motility. Many of the proteins that contribute to the unique microenvironment of the primate epididymis, and thus to sperm maturation, are dependent on androgens to induce their synthesis...
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1054/tice.2000.0164
更新日期：2001-06-01 00:00:00
abstract：:We studied the morphology of rabbit psoas muscle fixed at increasing intervals of time in a chemical skinning solution (Wood et al., 1975), or after skinning and storage for times up to 1 week. The storage solution, in which the chemically skinned muscled fibers were kept at -20 degrees C, had the same ionic compositi...
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1016/0040-8166(79)90062-4
更新日期：1979-01-01 00:00:00
abstract：:As part of an ongoing comparative fine structural study, the retinal pigment epithelium (RPE), choriocapillaris and complexus basalis (Bruch's membrane) in the emu (Dromaius novaehollandiae) have been investigated by light and electron microscopy. The RPE consists of a single layer of cuboidal cells joined basally by ...
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1016/s0040-8166(98)80063-3
更新日期：1998-04-01 00:00:00
abstract：:A panel of 40 monoclonal antibodies was constructed in response to cationic endothelial cell growth factor (c-ECGF), the cationic peptide mitogen isolated from endothelial mitogen. The monoclonal antibodies were assayed by dot blot for immunoreactivity to various other peptide angiogenic factors. The panel of monoclon...
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1016/0040-8166(87)90040-1
更新日期：1987-01-01 00:00:00
abstract：:This study evaluated ulceroprotective and antioxidant effect of 1,25 dihydroxyvitamin D3 against gastric damage in rats. Rats were treated intraperitoneally with either 1,25 dihydroxyvitamin D3 (0.25 μg/kg) or saline for 14 days. On day-15, the non-selective cyclooxygenase inhibitor indomethacin (10 mg/kg; subcutaneou...
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1016/j.tice.2018.03.003
更新日期：2018-04-01 00:00:00
abstract：:The number of epithelial cells comprising larval midgut of the tobacco hornworm moth, Manduca sexta increases 200-fold in development from the first to the fifth instar. We have examined larvae periodically before and during molting to follow epithelial cell proliferation and differentiation. The midgut epithelium in ...
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1016/0040-8166(91)90058-2
更新日期：1991-01-01 00:00:00
