Abstract:
:A panel of 40 monoclonal antibodies was constructed in response to cationic endothelial cell growth factor (c-ECGF), the cationic peptide mitogen isolated from endothelial mitogen. The monoclonal antibodies were assayed by dot blot for immunoreactivity to various other peptide angiogenic factors. The panel of monoclonal antibodies to c-ECGF exhibited complete cross-reactivity with pituitary fibroblast growth factor and sarcoma-derived growth factor. A group of 28 monoclonal antibodies was found to exhibit reactivity to anionic endothelial mitogen (a-ECGF), brain fibroblast growth factor, endothelial cell growth factor, and retina-derived growth factor. None of the monoclonal antibodies was found to react with epidermal growth factor or platelet-derived growth factor. These data provide an immunological basis for grouping heparin-binding endothelial cell growth factors into anionic and cationic groups.
journal_name
Tissue Celljournal_title
Tissue & cellauthors
Schelling ME,Hawker JR Jr,Granger HJdoi
10.1016/0040-8166(87)90040-1subject
Has Abstractpub_date
1987-01-01 00:00:00pages
463-7issue
4eissn
0040-8166issn
1532-3072pii
0040-8166(87)90040-1journal_volume
19pub_type
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