Immunochemical comparison of peptide angiogenic factors.

Abstract:

:A panel of 40 monoclonal antibodies was constructed in response to cationic endothelial cell growth factor (c-ECGF), the cationic peptide mitogen isolated from endothelial mitogen. The monoclonal antibodies were assayed by dot blot for immunoreactivity to various other peptide angiogenic factors. The panel of monoclonal antibodies to c-ECGF exhibited complete cross-reactivity with pituitary fibroblast growth factor and sarcoma-derived growth factor. A group of 28 monoclonal antibodies was found to exhibit reactivity to anionic endothelial mitogen (a-ECGF), brain fibroblast growth factor, endothelial cell growth factor, and retina-derived growth factor. None of the monoclonal antibodies was found to react with epidermal growth factor or platelet-derived growth factor. These data provide an immunological basis for grouping heparin-binding endothelial cell growth factors into anionic and cationic groups.

journal_name

Tissue Cell

journal_title

Tissue & cell

authors

Schelling ME,Hawker JR Jr,Granger HJ

doi

10.1016/0040-8166(87)90040-1

subject

Has Abstract

pub_date

1987-01-01 00:00:00

pages

463-7

issue

4

eissn

0040-8166

issn

1532-3072

pii

0040-8166(87)90040-1

journal_volume

19

pub_type

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