Evaluating the in vitro therapeutic effects of human amniotic mesenchymal stromal cells on MiaPaca2 pancreatic cancer cells using 2D and 3D cell culture model.


:Human amniotic mesenchymal stromal cells (hAMSCs) are considered as a population of multipotent cells. The molecular events associated with mesenchymal stromal cell (MSC)/tumor cell interactions should be studied to identify the role of MSCs on suppressing or inducing the key signaling pathways of tumor cells. Thus, designing therapeutic approaches is considered as important. In the present study, hAMSCs and MiaPaca2 cells were first cultured separately. In addition, both cell lines were co-cultured by using 0.4 μm pore sized transwell membranes in different times. Further, the RNA of the cells was extracted, and Bcl2, Bax, epidermal growth factor receptor (EGFR), c-Src, C-terminal Src Kinase (CSK), and SGK223 expression were analyzed through quantitative real time PCR. Furthermore, the total cell lysates of the cells were prepared and analyzed by using western blot. Based on the results, the expression of EGFR, c-Src, SGK223, and CSK in MiaPaca2 cells reduced after treating with hAMSCs. Notably, the cellular apoptosis of MiaPaca2 cells was induced in 2D cell culture system. Further, the anti-cancer activity of conditioned medium from hAMSCs was confirmed in a 3D cell culture model by using hanging drop technique. Finally, hAMSCs have inhibitory effects on pancreatic cancer cells and can be considered as a therapeutic way to suppress EGFR, c-Src, and SGK223, as the potent targets in cancer cell signaling.


Tissue Cell


Tissue & cell


Rahmani Z,Safari F




Has Abstract


2021-02-01 00:00:00












  • Brain macrophages in cerebellar cell cultures.

    abstract::Brain macrophages were studied in dispersed monolayer cultures of post-natal mouse cerebella. The phagocytotic activity, binding of immunoglobulins, presence and characteristics of nucleoside diphosphatases as well as argentophilia and lipid distribution were tested, with results similar to those for brain macrophages...

    journal_title:Tissue & cell

    pub_type: 杂志文章


    authors: Ditrich H

    更新日期:1986-01-01 00:00:00

  • Cytochemical investigations on tunic morphogenesis in the sea peach Halocynthia papillosa (Tunicata, Ascidiacea) 2: demonstration of proteins.

    abstract::In a previous paper, cellulose fibres were demonstrated in the larval, the metamorphosing, and the juvenile tunics. In this paper we used cytochemical methods and X-ray microanalysis to obtain additional information on tunic morphogenesis in Halocynthia papillosa. The chemical composition of the tunic evolves with its...

    journal_title:Tissue & cell

    pub_type: 杂志文章


    authors: Lübbering-Sommer B,Compère P,Goffinet G

    更新日期:1996-12-01 00:00:00

  • Postembryonic development of leucokinin-like immunoreactive neurons in the moth Spodoptera litura.

    abstract::Antiserum to leucokinin I, a neuropeptide originally isolated from the cockroach Leucophaea maderae, was used for immunocytochemical labeling of neurons in the brain and ventral ganglia of the moth Spodoptera litura during postembryonic development. In the ventral ganglia, leucokinin-like immunoreactivity begins to oc...

    journal_title:Tissue & cell

    pub_type: 杂志文章


    authors: Lee BH,Kang H,Kwon D,Park CI,Kim WK,Kim MY

    更新日期:1998-02-01 00:00:00

  • Distribution of spermatozoa in the utero-tubal junction and isthmus of pigs, and their relationship with the luminal epithelium after mating: a scanning electron microscope study.

    abstract::The epithelial cell morphology and distribution in the utero-tubal junction and isthmus of pigs was documented by scanning electron microscopy around ovulation. In animals mated at different times before slaughter, our observations confirmed that the utero-tubal junction and posterior part of the isthmus regulate the ...

    journal_title:Tissue & cell

    pub_type: 杂志文章


    authors: Fléchon JE,Hunter RH

    更新日期:1981-01-01 00:00:00

  • Immunolocalization of venom metalloproteases in venom glands of adult and of newborn snakes of Bothrops jararaca.

    abstract::Using immunoelectronmicroscopy we analyzed qualitative and quantitatively the intracellular distribution of bothropasin, hemorrhagic factor 2 (HF2) and hemorrhagic factor 3 (HF3) in the venom secretory cells from adult snakes in the active (7 days after venom extraction) and in the resting (without venom extraction fo...

    journal_title:Tissue & cell

    pub_type: 杂志文章


    authors: Carneiro SM,Assakura MT,Barrence FA,Cardoso SR,de Martins Camargo AC,Sesso A

    更新日期:2002-12-01 00:00:00

  • Histochemical specificity of cholinesterases to phenylthioacetate in differentiated neural tissues of insects and teleosts.

    abstract::Housefly brain cholinesterase was histochemically demonstrated to hydrolyse phenylthioacetate at a very high rate, similar in distribution to that previously reported for acetylthiocholine. However, teleost neural retina cholinesterase would not hydrolyse the aromatic substrate, but the enzyme did cleave acetylthiocho...

    journal_title:Tissue & cell

    pub_type: 杂志文章


    authors: Booth GM,Whitt GS

    更新日期:1970-01-01 00:00:00

  • Human periodontal ligament cells derived from deciduous teeth induce osteoclastogenesis in vitro.

    abstract::The receptor activator of NF-kappa B ligand (RANKL) and its decoy receptor, osteoprotegerin (OPG), are the important proteins involved in osteoclastogenesis. In this study, we investigated the expressions of RANKL and OPG in cultured human periodontal ligament cells derived from deciduous teeth (DPDL cells) and their ...

    journal_title:Tissue & cell

    pub_type: 杂志文章


    authors: Hasegawa T,Kikuiri T,Takeyama S,Yoshimura Y,Mitome M,Oguchi H,Shirakawa T

    更新日期:2002-02-01 00:00:00

  • Role of pyridoxine in GABA synthesis and degradation in the hippocampus.

    abstract::Pyridoxal-5'-phosphate, the active form of vitamin B6, is associated with activities of several enzymes and the treatment of various neurological disorders. Here, we investigated the effects of pyridoxine on the immunoreactivity and protein levels of γ-aminobutyric acid (GABA)-synthesizing and degradation enzymes such...

    journal_title:Tissue & cell

    pub_type: 杂志文章


    authors: Jung HY,Kwon HJ,Kim W,Nam SM,Kim JW,Hahn KR,Yoo DY,Yoon YS,Choi SY,Kim DW,Hwang IK

    更新日期:2019-12-01 00:00:00

  • Keratinization in the epidermis of amphibians and the lungfish: comparison with amniote keratinization.

    abstract::Keratinization in the epidermis of amphibians and the lungfish has been studied by electron microscopy, autoradiography and immunocytochemistry to determine whether histidine-rich proteins, filaggrin and loricrin are present. In the lungfish and amphibian tadpoles, anti-keratin antibodies (AE1 and AE3) stain the whole...

    journal_title:Tissue & cell

    pub_type: 杂志文章


    authors: Alibardi L

    更新日期:2001-10-01 00:00:00

  • Nurse cell-oocyte interaction: a new F-actin mesh associated with the microtubule-rich core of an insect ovariole.

    abstract::Fluorescent staining of Rhodnius prolixus ovarioles with rhodaminylphalloidin revealed an elaborate interconnecting meshwork of F-actin encasing the microtubule rich core. The thick actin struts have branches extending partially into the nurse cell lobes extending from the syncytical core. Basally the network ends at ...

    journal_title:Tissue & cell

    pub_type: 杂志文章


    authors: Huebner E,Gutzeit H

    更新日期:1986-01-01 00:00:00

  • Unusual light-reflecting pigment cells appear in the Xenopus neural tube culture system in the presence of guanosine.

    abstract::Isolation and culture of Xenopus laevis neural tubes resulted in differentiation of melanophores and iridophores from neural crest cells; the differentiated melanophores and iridophores were then maintained in culture for more than 6 months. Guanosine has been reported to promote reflecting platelet formation in melan...

    journal_title:Tissue & cell

    pub_type: 杂志文章


    authors: Fukuzawa T,Kikuchi Y

    更新日期:2018-10-01 00:00:00

  • Organ culture of adult cockroach CNS: ultrastructural and physiological characteristics.

    abstract::The abdominal nerve cord of adult Periplaneta americana has been maintained in culture for up to 5 weeks. The ultrastructural appearance of in vitro cords resembles that of in vivo cords although some degeneration of glia may occur during the first 2 weeks in culture. The glial cells most affected are those which make...

    journal_title:Tissue & cell

    pub_type: 杂志文章


    authors: Howes EA,Leech CA

    更新日期:1985-01-01 00:00:00

  • Anti-fibrotic effect of black tea (Camellia sinensis) extract in experimental pulmonary fibrosis.

    abstract::There is no effective therapy exists for Idiopathic pulmonary fibrosis (IPF) till now. Few studies have been done on protective effects of green tea in pulmonary fibrosis but there is no single report on black tea extract (BTE) in pulmonary fibrosis so far. This study aims to investigate the anti-fibrotic effect of BT...

    journal_title:Tissue & cell

    pub_type: 杂志文章


    authors: Chakraborty K,Dey A,Bhattacharyya A,Dasgupta SC

    更新日期:2019-02-01 00:00:00

  • Structure and development of the telotrophic ovariole in ensign scale insects (Hemiptera, Coccomorpha: Ortheziidae).

    abstract::The paired ovaries of young larva of the 3rd instar of Orthezia urticae are filled with numerous germ cell clusters that can be regarded as ovariole anlagen. Germ cells (cystocytes) belonging to one cluster form a rosette, in the centre of which a polyfusome occurs. Staining with rhodamine-phalloidin has revealed that...

    journal_title:Tissue & cell

    pub_type: 杂志文章


    authors: Szklarzewicz T

    更新日期:1997-02-01 00:00:00

  • Cytology of lymphomyeloid head kidney of Antarctic fishes Trematomus bernacchii (Nototheniidae) and Chionodraco hamatus (Channicthyidae).

    abstract::Species that live in extreme conditions have specially adapted physiology and tissue/organ organisation. The adaptation of lymphoid organs to low temperatures in polar species could be an original field of study, indicating how the immune system works under extreme conditions. In fishes, the head kidney is a key organ...

    journal_title:Tissue & cell

    pub_type: 杂志文章


    authors: Romano N,Ceccariglia S,Mastrolia L,Mazzini M

    更新日期:2002-04-01 00:00:00

  • Na(+)/K(+)-ATPase activity and immunocytochemical labeling in podobranchial filament and lamina of the freshwater crayfish Astacus leptodactylus Eschscholtz: evidence for the existence of sodium transport in the filaments.

    abstract::In the crayfish Astacus leptodactylus, the podobranch, bearing respiratory and ion-transporting filaments, is attached to the lamina. The Na(+)/K(+)-ATPase activity is higher in filaments than in lamina. Using a mouse monoclonal antibody directed against the alpha subunit of Na(+)/K(+)-ATPase, and indirect immunofluor...

    journal_title:Tissue & cell

    pub_type: 杂志文章


    authors: Barradas C,Wilson JM,Dunel-Erb S

    更新日期:1999-10-01 00:00:00

  • Is the Golgi apparatus the obligatory final step for lipoprotein secretion by intestinal cells?

    abstract::It is currently admitted that the synthesis and excretion of triglyceride-rich lipoproteins (chylomicrons and 'small chylomicrons') by intestinal epithelial cells involves the Golgi apparatus as an obligatory final step before exocytosis. The cells of the proximal intestine of the trout are an excellent model for inve...

    journal_title:Tissue & cell

    pub_type: 杂志文章


    authors: Vernier JM,Sire MF

    更新日期:1986-01-01 00:00:00

  • Induction of choriogenesis by 20-hydroxyecdysone in the German cockroach.

    abstract::Experiments in vitro have shown that 20-hydroxyecdysone (at a concentration of 0.2 and 2 microM and after 12 and 24 hr of incubation) is able to induce the precocious deposition of chorion materials by the follicular epithelium of young oocytes of the cockroach Blattella germanica. Since previous studies had shown tha...

    journal_title:Tissue & cell

    pub_type: 杂志文章


    authors: Bellés X,Cassier P,Cerdá X,Pascual N,André M,Rósso Y,Piulachs MD

    更新日期:1993-04-01 00:00:00

  • The final stages of wing development in Drosophila melanogaster.

    abstract::A brief description is given of the behavioural changes in Drosophila melanogaster at the time of eclosion and wing expansion. The developmental changes in the wing during this period are classified into four stages (0-III). Details of changes in the morphology of both the cuticle and the wing epidermal cells are give...

    journal_title:Tissue & cell

    pub_type: 杂志文章


    authors: Johnson SA,Milner MJ

    更新日期:1987-01-01 00:00:00

  • Vascular endothelium of human dental pulp expresses diverse adhesion molecules for leukocyte emigration.

    abstract::The expression of adhesion molecules on the vascular endothelium of healthy and inflamed human dental pulp was studied immunohistochemically using antibodies for selectin family and immunoglobulin super-family members. In healthy dental pulp, some vessels reacted very weakly with major histocompatibility complex (MHC)...

    journal_title:Tissue & cell

    pub_type: 杂志文章


    authors: Sawa Y,Yoshida S,Shibata KI,Suzuki M,Mukaida A

    更新日期:1998-04-01 00:00:00

  • Human chondrocyte cell lines from articular cartilage of metatarsal phalangeal joints.

    abstract::Chondrocytes can be isolated from human adult cartilage from metatarsal phalangeal joints. After enzymatic digestion to isolate viable cells, confluent monolayers were obtained 2-4 weeks after the start of cell division. Chondrocytes cultures, initiated and maintained in HAM's F12 with bovine fetal serum without the a...

    journal_title:Tissue & cell

    pub_type: 杂志文章


    authors: Patti AM,Gabriele A,Della Rocca C

    更新日期:1999-12-01 00:00:00

  • Nitric oxide stimulates embryonic somatotroph differentiation and growth hormone mRNA and protein expression through a cyclic guanosine monophosphate-independent mechanism.

    abstract::In the pituitary gland, NO is locally synthesized by gonadotroph and folliculo-stellate cells. Many reports have shown that NO can modulate the growth hormone (GH) secretion. However, its role on mice embryo GH regulation remains unclear. In addition, it is unknown whether the regulation is associated with the prolife...

    journal_title:Tissue & cell

    pub_type: 杂志文章


    authors: Chen HP,Zhang L,Xu BH,Kang LM,Yang SL,Zeng HH,Shi XY

    更新日期:2009-04-01 00:00:00

  • Evidence that Müller cells can phagocytize egg-lecithin-coated silicone particles.

    abstract::This study is designed to better understand the function of the Müller cells of the retina through the use of silicon particles that had been injected into the vitreous body of the eye and via an analysis of the number of gap junctions associated with these cells. Following intraocular injection of a silicone oil mixt...

    journal_title:Tissue & cell

    pub_type: 杂志文章


    authors: Nishizono H,Murata Y,Tanaka M,Soji T,Herbert DC

    更新日期:1993-01-01 00:00:00

  • The vitellin-processing protease of Blattella germanica is derived from a pro-protease of maternal origin.

    abstract::Proteolytic processing of vitellin in Blattella germanica embryos is accomplished by activation of a yolk-borne cysteine protease (Mr 29 000) derived from a pro-protease precursor of Mr 40 000 (Liu et al., 1997). In the present study, fat body, ovaries and embryos of different developmental stages were examined immuno...

    journal_title:Tissue & cell

    pub_type: 杂志文章


    authors: Giorgi F,Yin L,Cecchettini A,Nordin JH

    更新日期:1997-06-01 00:00:00

  • A comparative analysis of deep learning architectures on high variation malaria parasite classification dataset.

    abstract::Malaria, one of the leading causes of death in underdeveloped countries, is primarily diagnosed using microscopy. Computer-aided diagnosis of malaria is a challenging task owing to the fine-grained variability in the appearance of some uninfected and infected class. In this paper, we transform a malaria parasite objec...

    journal_title:Tissue & cell

    pub_type: 杂志文章


    authors: Rahman A,Zunair H,Reme TR,Rahman MS,Mahdy MRC

    更新日期:2020-12-31 00:00:00

  • Ultrastructure of the accessory glands of gene's organ in the cattle tick, Boophilus.

    abstract::The organization and ultrastructure of the accessory glands of the cattle tick, Boophilus microplus, are described. The glands consist of two groups of acinar cells situated on either side of Gene's organ. A single acinus consists of from eight to 12 cells and each cell is connected via an individual duct to pores on ...

    journal_title:Tissue & cell

    pub_type: 杂志文章


    authors: Booth TF,Beadle DJ,Hart RJ

    更新日期:1984-01-01 00:00:00

  • Evaluation of immunohistochemical markers of germ cells' proliferation in the developing rat testis: a comparative study.

    abstract::Germ cells' proliferation during testicular organogenesis in Wistar rat embryos and neonates [14.5, 18.5, 20.5 days post conception (dpc), birth (day 0), 1, 3, 5, 7 days post partum (dpp)] was evaluated via immunohistochemistry, using the PCNA and Ki-67 nuclear antibodies. Estimation of the reactive/total cell ratio, ...

    journal_title:Tissue & cell

    pub_type: 杂志文章


    authors: Angelopoulou R,Balla M,Lavranos G,Chalikias M,Kitsos C,Baka S,Kittas C

    更新日期:2008-02-01 00:00:00

  • Germ cell proliferation and apoptosis during testicular regression in a seasonal breeding fish kept in captivity.

    abstract::Cell proliferation and apoptosis regulate germ cells stock and sperm production, eliminate anomalous gametes, and are essential parameters to consider in fish farming. Herein, spermatogenic activity as well as germ cell proliferation and apoptosis were assessed in Leporinus taeniatus, a seasonal breeding species from ...

    journal_title:Tissue & cell

    pub_type: 杂志文章


    authors: Ribeiro YM,Matos SA,Domingos FFT,Santos HBD,Cruz Vieira AB,Bazzoli N,Rizzo E

    更新日期:2017-12-01 00:00:00

  • Azoramide ameliorates fructose-induced nonalcoholic fatty liver disease in mice.

    abstract::Nonalcoholic fatty liver disease (NAFLD) is a frequent health problem. The insulin resistance and endoplasmic reticulum (ER) stress have been suggested to play important roles in the development and progression of NAFLD. However these processes and correlations have not fully been understood yet. Azoramide, an antidia...

    journal_title:Tissue & cell

    pub_type: 杂志文章


    authors: Bagci R,Sahinturk V,Sahin E

    更新日期:2019-08-01 00:00:00

  • Ultrastructure of peritubular tissue in association with tubular hyalinization in human testis.

    abstract::Testicular peritubular tissue, also known as the tunica propria, surrounds the seminiferous tubules and is responsible for contractile, paracrine and transport functions. The aim of the present report is to describe the pathomorphology of peritubular tissue in association with tubular hyalinization in human testis. Tw...

    journal_title:Tissue & cell

    pub_type: 临床试验,杂志文章


    authors: Haider SG,Talati J,Servos G

    更新日期:1999-02-01 00:00:00