Evaluating the in vitro therapeutic effects of human amniotic mesenchymal stromal cells on MiaPaca2 pancreatic cancer cells using 2D and 3D cell culture model.


:Human amniotic mesenchymal stromal cells (hAMSCs) are considered as a population of multipotent cells. The molecular events associated with mesenchymal stromal cell (MSC)/tumor cell interactions should be studied to identify the role of MSCs on suppressing or inducing the key signaling pathways of tumor cells. Thus, designing therapeutic approaches is considered as important. In the present study, hAMSCs and MiaPaca2 cells were first cultured separately. In addition, both cell lines were co-cultured by using 0.4 μm pore sized transwell membranes in different times. Further, the RNA of the cells was extracted, and Bcl2, Bax, epidermal growth factor receptor (EGFR), c-Src, C-terminal Src Kinase (CSK), and SGK223 expression were analyzed through quantitative real time PCR. Furthermore, the total cell lysates of the cells were prepared and analyzed by using western blot. Based on the results, the expression of EGFR, c-Src, SGK223, and CSK in MiaPaca2 cells reduced after treating with hAMSCs. Notably, the cellular apoptosis of MiaPaca2 cells was induced in 2D cell culture system. Further, the anti-cancer activity of conditioned medium from hAMSCs was confirmed in a 3D cell culture model by using hanging drop technique. Finally, hAMSCs have inhibitory effects on pancreatic cancer cells and can be considered as a therapeutic way to suppress EGFR, c-Src, and SGK223, as the potent targets in cancer cell signaling.


Tissue Cell


Tissue & cell


Rahmani Z,Safari F




Has Abstract


2021-02-01 00:00:00












  • The Harderian gland of the Dhub lizard Uromastyx microlepis of the Kuwaiti desert: an ultrastructural approach.

    abstract::Harderian glands exist in the orbits of most terrestrial vertebrates. The basic function of the gland is the lubrication of the eye. The present study was carried out to shed some light on the ultrastructure of the still enigmatic Harderian gland of the lizard Uromastyx microlepis, a common species in Kuwait and other...

    journal_title:Tissue & cell

    pub_type: 杂志文章


    authors: Sabry I,Al-Ghaith L

    更新日期:2000-02-01 00:00:00

  • Osteogenesis induced by a three-dimensional bioimplant composed of demineralised bone matrix, collagen, hydroxyapatite, and bone marrow-derived cells in massive bone defects: An experimental study.

    abstract::Treatment of massive bone defects is one of the most difficult problems to solve in orthopedics. At present, there is no consensus on the best way to resolve these problems. The aim of our study was to evaluate the effect of a three-dimensional bioimplant over massive bone defects, and to analyse if it improves the sp...

    journal_title:Tissue & cell

    pub_type: 杂志文章


    authors: Cuervo-Lozano CE,Soto-Domínguez A,Saucedo-Cárdenas O,Montes-de-Oca-Luna R,Alonso-Romero S,Del Consuelo Mancías-Guerra M,Álvarez-Lozano E

    更新日期:2018-02-01 00:00:00

  • Role of pyridoxine in GABA synthesis and degradation in the hippocampus.

    abstract::Pyridoxal-5'-phosphate, the active form of vitamin B6, is associated with activities of several enzymes and the treatment of various neurological disorders. Here, we investigated the effects of pyridoxine on the immunoreactivity and protein levels of γ-aminobutyric acid (GABA)-synthesizing and degradation enzymes such...

    journal_title:Tissue & cell

    pub_type: 杂志文章


    authors: Jung HY,Kwon HJ,Kim W,Nam SM,Kim JW,Hahn KR,Yoo DY,Yoon YS,Choi SY,Kim DW,Hwang IK

    更新日期:2019-12-01 00:00:00

  • The same cell lineage is involved in scale formation and regeneration in the teleost fish Hemichromis bimaculatus.

    abstract::The elasmoid scales of the cichlid fish, Hemichromis bimaculatus, are localized within dermal pockets, the floors of which are separated from the stratum compactum by uninterrupted cellular sheets, the scale-pocket linings (SPL). TEM study of the fry skin shows that the SPL cells originate from the cell population con...

    journal_title:Tissue & cell

    pub_type: 杂志文章


    authors: Sire JY

    更新日期:1989-01-01 00:00:00

  • Intracisternal tubules and intramitochondrial filaments in cells of a snail, Limnaea.

    abstract::Epithelial and peritubular cells associated with the reproductive tract of the snail, Limnaea stagnalis, contain an extensive system of endoplasmic reticulum that is often dilated with many closely packed intracisternal tubules. The intracisternal tubules are approximately 24-28 nm in diameter and they are often hexag...

    journal_title:Tissue & cell

    pub_type: 杂志文章


    authors: Kessel RG,Beams HW

    更新日期:1984-01-01 00:00:00

  • Further evidence for secretion of matrix metalloproteinase-1 by Meckel's chondrocytes during degradation of the extracellular matrix.

    abstract::We examined the possibility that chondrocytes in Meckel's cartilage might secrete matrix metalloproteinase-1 (MMP-1) during degradation of the extracellular matrix. Evidence for the secretion of MMP-1 was obtained by immunohistochemical staining and immunoelectron microscopy, in addition to general histochemical stain...

    journal_title:Tissue & cell

    pub_type: 杂志文章


    authors: Ishizeki K,Nawa T

    更新日期:2000-06-01 00:00:00

  • Cuticular cavities: storage chambers for cyanoglucoside-containing defensive secretions in larvae of a Zygaenid moth.

    abstract::As a defensive reaction against predators the larvae of Zygaena trifolii Esper. 1783, release highly viscous fluid droplets out of cuticular cavities. The fluid appears on the cuticular surface upon contraction of the irritated segments, with no specialized muscles being involved. Two morphologically different types o...

    journal_title:Tissue & cell

    pub_type: 杂志文章


    authors: Franzl S,Naumann CM

    更新日期:1985-01-01 00:00:00

  • The influence of the donor on dental apical papilla stem cell properties.

    abstract::Stem cells from the human dental apical papilla (SCAP) can be obtained from almost all extracted wisdom teeth with an immature tooth root. Although different stem cell lines are used for studies, it remains elusive whether specific characteristics of the dental stem cell cultures such as proliferation rates or the cel...

    journal_title:Tissue & cell

    pub_type: 杂志文章


    authors: Prateeptongkum E,Klingelhöffer C,Morsczeck C

    更新日期:2015-08-01 00:00:00

  • The effects of castration followed testosterone supplementation in prostatic complex of Artibeus planirostris (Chiroptera: Phyllostomidae).

    abstract::The prostatic complex (ventral and dorsal regions) of Artibeus planirostris exhibits seasonal variations throughout the year. Circulating testosterone was correlated with prostate weight, showing an increase from autumn to summer, with the highest peak in summer corresponding to the largest breeding season. This indic...

    journal_title:Tissue & cell

    pub_type: 杂志文章


    authors: Puga CC,Beguelini MR,Morielle-Versute E,Vilamaior PS,Taboga SR

    更新日期:2016-06-01 00:00:00

  • Human periodontal ligament cells derived from deciduous teeth induce osteoclastogenesis in vitro.

    abstract::The receptor activator of NF-kappa B ligand (RANKL) and its decoy receptor, osteoprotegerin (OPG), are the important proteins involved in osteoclastogenesis. In this study, we investigated the expressions of RANKL and OPG in cultured human periodontal ligament cells derived from deciduous teeth (DPDL cells) and their ...

    journal_title:Tissue & cell

    pub_type: 杂志文章


    authors: Hasegawa T,Kikuiri T,Takeyama S,Yoshimura Y,Mitome M,Oguchi H,Shirakawa T

    更新日期:2002-02-01 00:00:00

  • An ultrastructural investigation of muscle attachment in the opercular filament of a polychaete annelid.

    abstract::The ultrastructure of peduncle muscle attachment to the cuticular flange in the opercular filament of the serpulid Pomatoceros lamarckii Quatrefages is described. The cuticular flange is composed of layers of orthogonally arranged fibres. Specialized epithelial cells (tendon cells) and a collagenous matrix intervene b...

    journal_title:Tissue & cell

    pub_type: 杂志文章


    authors: Bubel A

    更新日期:1983-01-01 00:00:00

  • Enhanced neogenic potential of Panc-1 cells supplemented with human umbilical cord blood serum--An alternative to FCS.

    abstract::The promise(s) of using Fetal Calf Serum (FCS) as a supplement for the maintenance of cell cultures has been well documented. However, FCS forms the xenogenic source for any human derived cells/organ and limits its application. Recently, the usage of human umbilical cord blood serum (hUCBS) for maintenance of mesenchy...

    journal_title:Tissue & cell

    pub_type: 杂志文章


    authors: Challa SS,Kiran GS,Bhonde RR,Venkatesan V

    更新日期:2011-08-01 00:00:00

  • Topography of the rat blood-testis barrier after intratubular administration of intercellular tracers.

    abstract::Intratubular injection of electron-opaque tracers (lanthanum hydroxide, peroxidase) by micromanipulation showed that the intercellular spaces of the adluminal compartment are in continuity with the lumen of the rat seminiferous tubule at all stages of the spermatogenic cycle. This continuity involves the intercellular...

    journal_title:Tissue & cell

    pub_type: 杂志文章


    authors: Cavicchia JC,Sacerdote FL

    更新日期:1988-01-01 00:00:00

  • Seminiferous epithelium cycle of a hantavirus reservoir, the long-tailed mouse Oligoryzomys flavescens (Rodentia-Cricetidae).

    abstract::Oligoryzomys flavescens, a common rodent of the temperate regions of South America, has been identified as a Hantavirus reservoir. There is still little information concerning its reproductive biology, which is essential to devise effective control measurement of natural populations. This rodent is a seasonal breeder ...

    journal_title:Tissue & cell

    pub_type: 杂志文章


    authors: Boiani L,Casanova G,Olivera A,Berois N

    更新日期:2007-08-01 00:00:00

  • Establishment and characterization of human pancreatic adenocarcinoma cell line in tissue culture and the nude mouse.

    abstract::We established a human pancreatic carcinoma cell line, designated SPH, from cancerous ascites of a 57-year-old male patient with ductal adenocarcinoma of the pancreas. The cells have been cultured for 32 months with RPMI-1640 medium supplemental with 10% fetal calf serum. The population doubling time of this cell line...

    journal_title:Tissue & cell

    pub_type: 杂志文章


    authors: Kong D,Nishino N,Shibusawa M,Kusano M

    更新日期:2007-08-01 00:00:00

  • Selective isolation of rat aortic wall layers and their cell types in culture--application to converting enzyme activity measurement.

    abstract::The rat aorta, whose three wall layers can be separated by microdissection offers the rare possibility of comparing physiological characteristics of in vivo tissular cell components and corresponding cells after culture. We developed a technique allowing the dissociation of the three tunicae (intima, media and adventi...

    journal_title:Tissue & cell

    pub_type: 杂志文章


    authors: Battle T,Arnal JF,Challah M,Michel JB

    更新日期:1994-12-01 00:00:00

  • Influence of several extracellular matrix components in primary cultures of bovine mammary epithelial cells.

    abstract::Mammary epithelial cells, obtained from lactating cows, were cultured onto inserts coated with several components of extracellular matrix. The influence of these components upon the maintenance of differentiation has been determinated. Every day, alpha S1-casein secretion was measured by radioimmunoassay (RIA) in apic...

    journal_title:Tissue & cell

    pub_type: 杂志文章


    authors: Delabarre S,Claudon C,Laurent F

    更新日期:1997-02-01 00:00:00

  • Argon plasma-treated fluorinated ethylene propylene: Growth of primary dermal fibroblasts and mesenchymal stem cells.

    abstract::Surface modification is an important step in making a synthetic polymer cytocompatible. We have previously reported improved cytocompatibility of immortalized human keratinocytes (HaCaT) with the otherwise bioinert fluorinated ethylene propylene (FEP) upon treatment with argon plasma discharge. In this article, we sho...

    journal_title:Tissue & cell

    pub_type: 杂志文章


    authors: Peterková L,Rimpelová S,Slepička P,Křížová I,Kasálková NS,Švorčík V,Ruml T

    更新日期:2019-06-01 00:00:00

  • Unusual organization of the tropharium in the telotrophic ovarioles of an insect, Saldula saltatoria.

    abstract::The tropharium of the common shorebug Saldula saltatoria consists of 2 zones: the apical mitotic region and the distal one comprising numerous mononucleate nurse cells. Each individual nurse cell is connected to the centrally located trophic core by a thin cytoplasmic projection referred to as a trophic process. The a...

    journal_title:Tissue & cell

    pub_type: 杂志文章


    authors: Biliński SM,Ksiazkiewicz-Kapralska M,Stys P

    更新日期:1990-01-01 00:00:00

  • The effect of fibrin on cultured vascular endothelial cells.

    abstract::The normal cobblestone monolayer architecture of cultured vascular endothelium becomes rapidly disorganized after contact of the cell layer with a fibrin clot. The cells of a confluent endothelial monolayer separate into individual migratory cells in 4--6 hr after contact with fibrin. The effect is reversible in that ...

    journal_title:Tissue & cell

    pub_type: 杂志文章


    authors: Kadish JL,Butterfield CE,Folkman J

    更新日期:1979-01-01 00:00:00

  • Duration of spermatogenesis and sperm transit time through the epididymis in the Piau boar.

    abstract::The Piau boar is a rustic breed of economical importance in Brazil. The duration of spermatogenesis and sperm transit through the epididymis in Piau boars was estimated using intratesticular injections of tritiated thymidine. Animals were sacrificed 1 h, 7 days, 14 days, 21 days, 34 days, and 36 days after injections....

    journal_title:Tissue & cell

    pub_type: 杂志文章


    authors: França LR,Cardoso FM

    更新日期:1998-10-01 00:00:00

  • Absorption of phosphorus from the spermatophore through the cuticle of the bursa copulatrix of the butterfly, Calpodes ethlius.

    abstract::In lepidoptera the spermatophore is deposited in a bursa copulatrix, which is lined by a cuticle through which solubilized components of the spermatophore must be absorbed by the underlying epithelium. This study follows the route taken by the phosphorus which, with calcium, forms a significant portion of the spermato...

    journal_title:Tissue & cell

    pub_type: 杂志文章


    authors: Lai-Fook J

    更新日期:1991-01-01 00:00:00

  • Lenticles: innervated secretory structures that are expressed at every other larval moult.

    abstract::Lenticles are dome-shaped circles or ovals of cuticle with a dark rim. They occur with a precise segmental arrangement in the larvae and pupae of lycaenid and hesperiid butterflies. In Calpodes ethlius (Lepidoptera, Hesperiidae) each lenticle is secreted by a pair of large polyploid epidermal cells. The dark rim or an...

    journal_title:Tissue & cell

    pub_type: 杂志文章


    authors: Franzl S,Locke M,Huie P

    更新日期:1984-01-01 00:00:00

  • Extracellular and intracellular degradation of collagen by trophoblast giant cells in acute fasted mice examined by electron microscopy.

    abstract::The fine structure of trophoblast giant cells and their interaction with collagen at the antimesometrial region on the 9th day of pregnancy was examined in fed and acute fasted mice. Collagen fibrils and filamentous aggregates (disintegrating collagen fibrils) were observed in the extracellular space. Three types of i...

    journal_title:Tissue & cell

    pub_type: 杂志文章


    authors: Katz SG

    更新日期:1995-12-01 00:00:00

  • Evidence for multiple satellite cell populations and a non-myogenic cell type that is regulated differently in regenerating and growing skeletal muscle.

    abstract::We have performed studies to determine if different populations of satellite cells provide nuclei to growing and regenerating skeletal muscle fibers. Satellite cells were isolated from regenerating or growing anterior tibialis muscles, and their phenotypic properties were compared in vitro. Isolates from regenerating ...

    journal_title:Tissue & cell

    pub_type: 杂志文章


    authors: Molnar G,Ho ML,Schroedl NA

    更新日期:1996-10-01 00:00:00

  • Evaluation of immunohistochemical markers of germ cells' proliferation in the developing rat testis: a comparative study.

    abstract::Germ cells' proliferation during testicular organogenesis in Wistar rat embryos and neonates [14.5, 18.5, 20.5 days post conception (dpc), birth (day 0), 1, 3, 5, 7 days post partum (dpp)] was evaluated via immunohistochemistry, using the PCNA and Ki-67 nuclear antibodies. Estimation of the reactive/total cell ratio, ...

    journal_title:Tissue & cell

    pub_type: 杂志文章


    authors: Angelopoulou R,Balla M,Lavranos G,Chalikias M,Kitsos C,Baka S,Kittas C

    更新日期:2008-02-01 00:00:00

  • Lectin histochemistry on the skin of hairless descendants of Mexican hairless dogs.

    abstract::The skin of hairless descendants of Mexican hairless dogs was investigated lectin-histochemically. Haired dogs were also used for comparative study. In the epidermis of infant pups, lectin staining revealed marked differences between hairless and haired dogs. Griffonia simplicifolia-I (GS-I) bound moderately to the cy...

    journal_title:Tissue & cell

    pub_type: 杂志文章


    authors: Miyazawa M,Kimura T,Itagaki S

    更新日期:1994-02-01 00:00:00

  • Comparative ultrastructure of catch tentacles and feeding tentacles in the sea anemone Haliplanella.

    abstract::TEM observations of catch tentacles revealed that the tentacle tip epidermis is filled with two size classes of mature holotrich nematocysts and a gland cell filled with electron-dense vesicles. Vesicle production is restricted to upper-middle and tentacle tip regions, whereas holotrich development occurs in the lower...

    journal_title:Tissue & cell

    pub_type: 杂志文章


    authors: Watson GM,Mariscal RN

    更新日期:1983-01-01 00:00:00

  • Endocrine cells and nerves in the stomach of the lizard Podarcis hispanica detected by immunocytochemistry.

    abstract::The general identification of endocrine cells in the stomach of the lizard Podarcis hispanica was carried out by their response to the Grimelius and Masson-Fontana techniques. 11 immunoreactive cell-types, positive for chromogranin-, serotonin-, caerulein/gastrin/ cholecystokinin (CAER/G/CCK)-, glucagon-like-peptide-1...

    journal_title:Tissue & cell

    pub_type: 杂志文章


    authors: De Rada OD,Burrell MA,García C,Barrenechea MA,Sesma P

    更新日期:1992-01-01 00:00:00

  • Salivary gland of the tick vector of East Coast fever. IV. Cell type selectivity and host cell responses to Theileria parva.

    abstract::Responses of cells in the tick salivary gland to parasitism by Theileria parva were studied by electron microscopy. The gland is composed of three distinct types of acini (I, II, III) which together include ten or more different cell types. Of some 30 infected cells observed in the present study, all were E-cells of a...

    journal_title:Tissue & cell

    pub_type: 杂志文章


    authors: Fawcett DW,Büscher G,Doxsey S

    更新日期:1982-01-01 00:00:00