A feasible method for the isolation of mesenchymal stem cells from menstrual blood and their exosomes.


BACKGROUND:Mesenchymal stem cells (MSCs) are currently the most promising candidates in regenerative medicine. Nonetheless, there are several limitations associated with the MSC tissue source such as infrequent and invasive bone marrow sampling methods. To overcome these limitations, we have procured MSCs from the menstrual blood (MenSCs) as a non-invasive source using a straightforward and cost-effective method. Moreover, we isolated MenSCs-derived exosomes as a safe and highly effective approach to exert the paracrine effects of MSCs. METHODS:MSCs were isolated from menstrual blood through two different culture methods: ficoll-isolated mononuclear cells (MNCs) and whole blood culture. These cells were characterized by their plastic adherence, flow cytometry analysis of the surface markers and the differentiation potential. The exosomes were isolated from conditioned media using ultracentrifugation and characterized by different microscopy techniques, western blotting, and ELISA. RESULTS:Both Methods resulted in the rapid isolation of cells with MSC properties. However, the cellular yield of the whole blood culture method was remarkably more than MNCs culture. MenSCs also produced a substantial amount of extracellular vesicles (EVs) possessed the minimum criteria for exosome definition. CONCLUSION:The results suggest that direct culture of whole menstrual blood cells is a high throughput, straightforward and cost-effective method for MenSCs isolation using no special growth factors. Moreover, these cells are a good producer of exosome which can offer a cell-free therapy approach.


Tissue Cell


Tissue & cell


Dalirfardouei R,Jamialahmadi K,Mahdipour E




Has Abstract


2018-12-01 00:00:00












  • Atypical 'fibrillar' flight muscle in strepsiptera.

    abstract::The fine structure of the principal and ancillary metathoracic flight muscle fibres in the adult male of a strepsipteran, Elenchus tenuicornis, is described. Power-producing dorsal longitudinal and dorso-ventral flight muscles show features consistent with myoneural asynchrony: myofibrils are large and discrete and ar...

    journal_title:Tissue & cell

    pub_type: 杂志文章


    authors: Smith DS,Kathirithamby J

    更新日期:1984-01-01 00:00:00

  • Morphological and ultrastructural analysis of Turritopsis nutricula during life cycle reversal.

    abstract::The hydrozoa life cycle is characterized, in normal conditions, by the alternation of a post-larval benthic polyp and an adult pelagic medusa; however, some species of Hydrozoa react to environmental stress by reverting their life cycle: i.e. an adult medusa goes back to the juvenile stage of polyp. This very uncommon...

    journal_title:Tissue & cell

    pub_type: 杂志文章


    authors: Carla' EC,Pagliara P,Piraino S,Boero F,Dini L

    更新日期:2003-06-01 00:00:00

  • A morphological study on gills of the brown shrimp, Penaeus aztecus.

    abstract::The gills of Penaeus aztecus were examined by light and electron microscopy. They are dendrobranchiate, consisting of a central axis with biserially arranged branches that subdivide into bifurcating filaments. A septum divides the lumina of these structures into afferent and efferent channels. Hemolymph from the stern...

    journal_title:Tissue & cell

    pub_type: 杂志文章


    authors: Foster CA,Howse HD

    更新日期:1978-01-01 00:00:00

  • Unusual organization of the tropharium in the telotrophic ovarioles of an insect, Saldula saltatoria.

    abstract::The tropharium of the common shorebug Saldula saltatoria consists of 2 zones: the apical mitotic region and the distal one comprising numerous mononucleate nurse cells. Each individual nurse cell is connected to the centrally located trophic core by a thin cytoplasmic projection referred to as a trophic process. The a...

    journal_title:Tissue & cell

    pub_type: 杂志文章


    authors: Biliński SM,Ksiazkiewicz-Kapralska M,Stys P

    更新日期:1990-01-01 00:00:00

  • Recycling of mast cells following degranulation in vitro: an ultrastructural study.

    abstract::Mature mast cells, isolated from the rat peritoneal cavity, were placed into suspension culture, either as resting or after degranulation by exposure to compound 48/80, and were maintained for up to 63 hr. No mitotic cells were observed, and cell number was conserved. The culture conditions did not cause spontaneous d...

    journal_title:Tissue & cell

    pub_type: 杂志文章


    authors: Burwen SJ

    更新日期:1982-01-01 00:00:00

  • Partial development of the steroidogenic ultrastructural features in degenerative corpora lutea after a single injection of pituitary extract in the Western painted turtle (Chrysemys picta).

    abstract::Pituitary glands were removed from sexually mature female turtles (Chrysemys picta) and they were injected intraperitoneally (i.p.) into other mature females of the same species (experimental). In addition mature females of the same species received saline injection only (controls). Initially all the turtles used in t...

    journal_title:Tissue & cell

    pub_type: 杂志文章


    authors: Mahmoud IY,Ba-Omar T,Alkindi A

    更新日期:2006-06-01 00:00:00

  • Galanin: presence and distribution in the brain and pituitary of Rhinella arenarum (Amphibia: Anura) during development.

    abstract::The immunohistochemical distribution of galanin (Gal) in the brain and pituitary of Rhinella arenarum was studied during development. Gal-immunoreactivity was first observed in the brain just after hatching in anterior preoptic area, infundibular area, median eminence and pars distalis of the pituitary as well as in t...

    journal_title:Tissue & cell

    pub_type: 杂志文章


    authors: Heer T,Yovanovich CA,Pozzi AG,Paz DA

    更新日期:2008-10-01 00:00:00

  • Blockade of the hypothalamic-pituitary-testicular axis with a GnRH antagonist in the neonatal marmoset monkey: changes in Leydig cell ultrastructure.

    abstract::Little is known of the cell biology of Leydig cells during the neonatal activation of the hypothalamic-pituitary-testicular (HPT) axis. The current study examined the effect of blockade of the HPT axis with a GnRH antagonist (antide) on the neonatal population of Leydig cells in the new world primate, the common marmo...

    journal_title:Tissue & cell

    pub_type: 杂志文章


    authors: Prince FP,Mann DR,Fraser HM

    更新日期:1998-12-01 00:00:00

  • The effects of castration followed testosterone supplementation in prostatic complex of Artibeus planirostris (Chiroptera: Phyllostomidae).

    abstract::The prostatic complex (ventral and dorsal regions) of Artibeus planirostris exhibits seasonal variations throughout the year. Circulating testosterone was correlated with prostate weight, showing an increase from autumn to summer, with the highest peak in summer corresponding to the largest breeding season. This indic...

    journal_title:Tissue & cell

    pub_type: 杂志文章


    authors: Puga CC,Beguelini MR,Morielle-Versute E,Vilamaior PS,Taboga SR

    更新日期:2016-06-01 00:00:00

  • Acute effects of rat growth hormone (GH), human GH and prolactin on proliferating rat liver cells in vitro: a study of mitotic behaviour and ultrastructural alterations.

    abstract::Examination of livers from transgenic mice over expressing human growth hormone (hGH) revealed numerous alterations including a striking incidence of mitotic figures. The reason for increased proliferation is unclear, but could be related to effects of hGH, which is also acting as a lactogen in rodents. In order to id...

    journal_title:Tissue & cell

    pub_type: 杂志文章


    authors: Vergani G,Mayerhofer A,Bartke A

    更新日期:1994-06-01 00:00:00

  • Surface localization of wheat germ agglutinin and concanavalin A binding sites on normal human blood cells: an ultrastructural histochemical study.

    abstract::In order to determine the extent and variations in surface concavanalin A (CON A) and wheat germ agglutinin (WGA) labeling of different varieties of normal blood cells, gluraraldehyde-fixed human blood cells were exposed to CON A-gold labeled horseradish peroxidase (CON A-HRP-G) and WGA-gold labeled ovomucoid (WGA-OVO...

    journal_title:Tissue & cell

    pub_type: 杂志文章


    authors: Zinsmeister VA,Ackerman GA

    更新日期:1983-01-01 00:00:00

  • Immunochemical comparison of peptide angiogenic factors.

    abstract::A panel of 40 monoclonal antibodies was constructed in response to cationic endothelial cell growth factor (c-ECGF), the cationic peptide mitogen isolated from endothelial mitogen. The monoclonal antibodies were assayed by dot blot for immunoreactivity to various other peptide angiogenic factors. The panel of monoclon...

    journal_title:Tissue & cell

    pub_type: 杂志文章


    authors: Schelling ME,Hawker JR Jr,Granger HJ

    更新日期:1987-01-01 00:00:00

  • Rat heart-derived endothelial and smooth muscle cell cultures: isolation, cloning and characterization.

    abstract::This report describes the initiation, cloning and establishment of long-term serial cultures of rat heart-derived vascular endothelial (EC) and smooth muscle cells (SMC). Populations of these cells derived from both the macro-and microcirculation were obtained utilizing isolated heart perfusion technique. Elimination ...

    journal_title:Tissue & cell

    pub_type: 杂志文章


    authors: Diglio CA,Grammas P,Giacomelli F,Wiener J

    更新日期:1988-01-01 00:00:00

  • The injury response of the neurones of Periplaneta Americana.

    abstract::Bilateral pairs of specific neurones in the metathoracic ganglion of the cockroach were used to study the ultrastructural changes which follow axon injury. The appearance of these matched pairs of cells was compared three days after the axons on one side of the ganglion only had been severed. The control or uninjured ...

    journal_title:Tissue & cell

    pub_type: 杂志文章


    authors: Young D,Ashhurst DE,Cohen MJ

    更新日期:1970-01-01 00:00:00

  • Structure of the subgenual organ in the green lacewing, Chrysoperla carnea.

    abstract::REM and TEM studies of the subgenual organ in Chrysoperla carnea (Neuroptera: Chrysopidae) show that it is composed of three scolopidia, each with one sensory, one scolopale and one cap cell. The distal part of the dendrite shows a cilium with a '9 + 0' structure. The cross-handing pattern of the ciliary root has a pe...

    journal_title:Tissue & cell

    pub_type: 杂志文章


    authors: Devetak D,Pabst MA

    更新日期:1994-04-01 00:00:00

  • Morphogenesis of the antenna of the male silkmoth, Antheraea polyphemus. V. Development of the peripheral nervous system.

    abstract::The imaginal antenna of the male silkmoth Antheraea polyphemus is a feather-shaped structure consisting of about 30 flagellomeres, each of which gives off two pairs of side branches. During the pupal stage (lasting for 3 weeks), the antenna develops from a leaf-shaped, flattened epidermal sac ('antennal blade') via tw...

    journal_title:Tissue & cell

    pub_type: 杂志文章


    authors: Steiner C,Keil TA

    更新日期:1995-06-01 00:00:00

  • Protein profiling and angiogenic effect of hypoxia-cultured human umbilical cord blood-derived mesenchymal stem cells in hindlimb ischemia.

    abstract::The aim of the present study was to investigate protein profiles of human umbilical cord blood-derived mesenchymal stem cells (hUCB-MSCs) cultured in normoxic (21% O2) and hypoxic (1% O2) conditions, and evaluate oxygenation effects on angiogenesis in an ischemic hindlimb mouse model using a modified ischemic scoring ...

    journal_title:Tissue & cell

    pub_type: 杂志文章


    authors: Han KH,Kim AK,Kim MH,Kim DH,Go HN,Kang D,Chang JW,Choi SW,Kang KS,Kim DI

    更新日期:2017-12-01 00:00:00

  • The same cell lineage is involved in scale formation and regeneration in the teleost fish Hemichromis bimaculatus.

    abstract::The elasmoid scales of the cichlid fish, Hemichromis bimaculatus, are localized within dermal pockets, the floors of which are separated from the stratum compactum by uninterrupted cellular sheets, the scale-pocket linings (SPL). TEM study of the fry skin shows that the SPL cells originate from the cell population con...

    journal_title:Tissue & cell

    pub_type: 杂志文章


    authors: Sire JY

    更新日期:1989-01-01 00:00:00

  • Epixenosomes: peculiar epibionts of the ciliate Euplotidium itoi: morphological and functional cell compartmentalization.

    abstract::As already reported, epixenosomes have a complex cell organization although they lack membranous organelles and a nuclear envelope. Different morphological compartments have been evidenced: (1) a space (IM) between the 2 external membranes in which acid phosphatase has been observed; (2) an apical, electrondense zone ...

    journal_title:Tissue & cell

    pub_type: 杂志文章


    authors: Rosati G,Giambelluca MA,Taiti E

    更新日期:1996-06-01 00:00:00

  • Ultrastructural observations of spermatozoa and spermiogenesis in Wandella orana Gray, 1994 (Araneae: Filistatidae) with notes on their phylogenetic implications.

    abstract::Spermatozoa and spermiogenesis of the prithine filistatid spider Wandella orana are described. The spider produces coenospermia, i.e. sperm aggregations that include several single sperm cells commonly surrounded by a secretion sheath. One sectioned coenospermium in W. orana contains at least five spermatozoa. During ...

    journal_title:Tissue & cell

    pub_type: 杂志文章


    authors: Michalik P,Gray MR,Alberti G

    更新日期:2003-10-01 00:00:00

  • Ultrastructural localization of calcium, by electron-probe X-ray microanalysis, in the small intestine of suckling rats.

    abstract::The subcellular distribution of calcium has been investigated in samples, from the intestinal mucosa of 10-day rats, prepared for X-ray microanalysis by various techniques designed to minimize the loss of this element. Calcium retention and its threshold of detection was most satisfactory in freeze-dried frozen thin s...

    journal_title:Tissue & cell

    pub_type: 杂志文章


    authors: Dinsdale D

    更新日期:1983-01-01 00:00:00

  • Alveolar type II cells: studies on the mode of release of lamellar bodies.

    abstract::There is increasing evidence that type II alveolar cells are capable of synthesizing surface active material like that obtained from the airways. However a number of problems remain to be solved before it can be stated conclusively that type II cells synthesize the surface active material of the terminal airspace. Amo...

    journal_title:Tissue & cell

    pub_type: 杂志文章


    authors: Ryan US,Ryan JW,Smith DS

    更新日期:1975-01-01 00:00:00

  • The unique organization of filamentous actin in the medullary canal of the medulla oblongata.

    abstract::In the central canal, F-actin is predominantly localized in the apical region, forming a ring-like structure around the circumference of the lumen. However, an exception is found in the medulla oblongata, where the apical F-actin becomes interrupted in the ventral aspect of the canal. To clarify the precise localizati...

    journal_title:Tissue & cell

    pub_type: 杂志文章


    authors: Tan BH,Guo CY,Xiong TQ,Chen LM,Li YC

    更新日期:2017-04-01 00:00:00

  • Morphological behavior of cultured bovine adrenal medulla capillary endothelial cells.

    abstract::Bovine adrenal medulla capillary endothelial cells were isolated and cloned, and their morphological behaviors in vitro were examined. In the culture of primary or early passage, one type of colony formed intracellular lumina both on the dish and in the three dimensional collagen gel. Another type proliferated well an...

    journal_title:Tissue & cell

    pub_type: 杂志文章


    authors: Furuya S,Edwards C,Ornberg R

    更新日期:1990-01-01 00:00:00

  • The fine structure of pigeon breast muscle.

    abstract::The pectoralis major muscle of the pigeon is composed of two types of muscle fibre. In the Type I fibres, the myofibrils are closely packed and there are few mitochondria. The myofibrils in the Type II fibres are separated by numerous columns of large mitochondria and lipid droplets. The membrane systems of the two ty...

    journal_title:Tissue & cell

    pub_type: 杂志文章


    authors: Ashhurst DE

    更新日期:1969-01-01 00:00:00

  • Microtubules and the development of the corkscrew region of a mealybug sperm bundle.

    abstract::Two systems of microtubules appear to be functionally significant in the transformation of a small cell into an elongated, acellular 'corkscrew', which represents the anterior region of a sperm bundle of Pseudococcus obscurus. One system of microtubules lies within the cell; it is oriented parallel to the long axis of...

    journal_title:Tissue & cell

    pub_type: 杂志文章


    authors: Ross J

    更新日期:1971-01-01 00:00:00

  • Seasonal variations in serotonin immunoreactivity and ultrastructure in the pineal organ of the Japanese grass lizard, with special reference to environmental temperature.

    abstract::The seasonal variations in serotonin immunoreactivity and ultrastructure of the secretory rudimentary photoreceptor cells (SRPC) were studied in the pineal organ of the Japanese grass lizard, Takydromus tachydromoides in relation to the environmental temperature. Our results clearly demonstrated that serotonin immunor...

    journal_title:Tissue & cell

    pub_type: 杂志文章


    authors: Ohshima K,Hirai S,Hiramatsu K

    更新日期:1999-08-01 00:00:00

  • Morphological and ultrastructural analysis of an important place of sexual communication of Rhodnius prolixus (Heteroptera: Reduviidae): the Metasternal Glands.

    abstract::Rhodnius prolixus is an important vector of Trypanosoma cruzi, the etiological agent of Chagas disease. Insect adults have a pair of Metasternal Glands (MGs) and the secretion emitted by these glands acts as sex pheromone. Recent studies have focused on the chemical composition of this pheromone, electrophysiological ...

    journal_title:Tissue & cell

    pub_type: 杂志文章


    authors: Lopes RL,Santos-Mallet JR,Barbosa CF,Gomes SAO,Spiegel CN

    更新日期:2020-12-01 00:00:00

  • Topography of the rat blood-testis barrier after intratubular administration of intercellular tracers.

    abstract::Intratubular injection of electron-opaque tracers (lanthanum hydroxide, peroxidase) by micromanipulation showed that the intercellular spaces of the adluminal compartment are in continuity with the lumen of the rat seminiferous tubule at all stages of the spermatogenic cycle. This continuity involves the intercellular...

    journal_title:Tissue & cell

    pub_type: 杂志文章


    authors: Cavicchia JC,Sacerdote FL

    更新日期:1988-01-01 00:00:00

  • Fine structure of tarsal sensory organs in the whip spider Admetus pumilio (Amblypygi, Arachnida).

    abstract::The sensory organs on the tarsi of the antenniform first legs of the whip spider Admetus pumilio C. L. Koch (Amblypygi, Arachnida) were examined with the scanning and transmission electron microscope. At least four different types of hair sensilla were found: (1) thick-walled bristles, which have the characteristics o...

    journal_title:Tissue & cell

    pub_type: 杂志文章


    authors: Foelix RF,Chu-Wang IW,Beck L

    更新日期:1975-01-01 00:00:00