Abstract:
BACKGROUND:Mesenchymal stem cells (MSCs) are currently the most promising candidates in regenerative medicine. Nonetheless, there are several limitations associated with the MSC tissue source such as infrequent and invasive bone marrow sampling methods. To overcome these limitations, we have procured MSCs from the menstrual blood (MenSCs) as a non-invasive source using a straightforward and cost-effective method. Moreover, we isolated MenSCs-derived exosomes as a safe and highly effective approach to exert the paracrine effects of MSCs. METHODS:MSCs were isolated from menstrual blood through two different culture methods: ficoll-isolated mononuclear cells (MNCs) and whole blood culture. These cells were characterized by their plastic adherence, flow cytometry analysis of the surface markers and the differentiation potential. The exosomes were isolated from conditioned media using ultracentrifugation and characterized by different microscopy techniques, western blotting, and ELISA. RESULTS:Both Methods resulted in the rapid isolation of cells with MSC properties. However, the cellular yield of the whole blood culture method was remarkably more than MNCs culture. MenSCs also produced a substantial amount of extracellular vesicles (EVs) possessed the minimum criteria for exosome definition. CONCLUSION:The results suggest that direct culture of whole menstrual blood cells is a high throughput, straightforward and cost-effective method for MenSCs isolation using no special growth factors. Moreover, these cells are a good producer of exosome which can offer a cell-free therapy approach.
journal_name
Tissue Celljournal_title
Tissue & cellauthors
Dalirfardouei R,Jamialahmadi K,Mahdipour Edoi
10.1016/j.tice.2018.09.010subject
Has Abstractpub_date
2018-12-01 00:00:00pages
53-62eissn
0040-8166issn
1532-3072pii
S0040-8166(18)30087-9journal_volume
55pub_type
杂志文章相关文献
TISSUE & CELL文献大全abstract::Oligoryzomys flavescens, a common rodent of the temperate regions of South America, has been identified as a Hantavirus reservoir. There is still little information concerning its reproductive biology, which is essential to devise effective control measurement of natural populations. This rodent is a seasonal breeder ...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/j.tice.2007.05.001
更新日期:2007-08-01 00:00:00
abstract::This report describes the initiation, cloning and establishment of long-term serial cultures of rat heart-derived vascular endothelial (EC) and smooth muscle cells (SMC). Populations of these cells derived from both the macro-and microcirculation were obtained utilizing isolated heart perfusion technique. Elimination ...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/0040-8166(88)90051-1
更新日期:1988-01-01 00:00:00
abstract::Polychlorinated biphenyls (PCBs) are worldwide pollutants and have caused hazardous effects on many animal species including humans. They have been detected in human milk and therefore exposure of newborns to PCBs is unavoidable if they are breast-fed. We present our findings on two experiments performed to test the e...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1054/tice.2000.0168
更新日期:2001-04-01 00:00:00
abstract::The epithelium of the gastric mucosa and its glands in the corpus of rat stomach contains mucous surface cells (MSCs), parietal cells, mucous neck cells (MNCs), zymogenic or chief cells (ZCs), several types of enteroendocrine cells, and intermediate cells with characteristics between MNCs and ZCs also called transitio...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/j.tice.2017.10.007
更新日期:2017-12-01 00:00:00
abstract::The expression and localization of gap junctional proteins connexin (Cx) 26, 32, and 43 was examined in human dental pulp. Dental pulp tissues were obtained from human third molars immediately after extraction. Some pulp tissues were used for cell culture, and the rest for histological observations. Immunostaining for...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/s0040-8166(02)00028-9
更新日期:2002-06-01 00:00:00
abstract::Embryonic stem cells (ESCs) have various uses in drug toxicity, as they can be easily differentiated in vitro. However, one of the major obstacles in the assessment of these differentiated cells is the presence of a heterogeneous cell population. To circumvent this problem, purified ESC-derived desired cells by means ...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/j.tice.2012.09.009
更新日期:2013-04-01 00:00:00
abstract::Megalocytivirus infection is a major threat in rock bream aquaculture in Korea. To produce a highly concentrated megalocytivirus, primary cells, established cell line and persistently infected cell line were used in this study. Megalocytivirus was inoculated in primary fin cell cultures of red sea bream (Pagrus major)...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/j.tice.2020.101387
更新日期:2020-10-01 00:00:00
abstract::Inflammatory bowel disease (IBD) is a complex immunological disorder characterized by chronic inflammation caused mainly by unknown factors. The interleukin-10 knockout (IL-10 KO) mouse is a well-established murine model of IBD which develops spontaneous intestinal inflammation that resembles Crohn's disease. In the p...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/j.tice.2014.12.001
更新日期:2015-02-01 00:00:00
abstract::To develop means of measuring angiotensin converting enzyme of endothelial cells in culture, we have synthesized benzoyl-Phe-Ala-Pro-OH (I), benzoyl-Pro-Phe-Arg-OH (II) and benzoyl-Gly-His-Leu-OH (III), each bearing a 3H-atom on the para-position of its benzoyl moiety. All three of the acylated tripeptides are substra...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/s0040-8166(16)30348-2
更新日期:1978-01-01 00:00:00
abstract::Morphological and functional changes caused by diabetes in the accessory sex organs and especially the prostate have been reported by several investigators. The aim of the present study was to examine the possible deleterious effects of experimentally induced diabetes on the secretory epithelium of the ventral prostat...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1054/tice.2000.0123
更新日期:2000-08-01 00:00:00
abstract::In order to determine the extent and variations in surface concavanalin A (CON A) and wheat germ agglutinin (WGA) labeling of different varieties of normal blood cells, gluraraldehyde-fixed human blood cells were exposed to CON A-gold labeled horseradish peroxidase (CON A-HRP-G) and WGA-gold labeled ovomucoid (WGA-OVO...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/0040-8166(83)90042-3
更新日期:1983-01-01 00:00:00
abstract::Human amniotic mesenchymal stromal cells (hAMSCs) are considered as a population of multipotent cells. The molecular events associated with mesenchymal stromal cell (MSC)/tumor cell interactions should be studied to identify the role of MSCs on suppressing or inducing the key signaling pathways of tumor cells. Thus, d...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/j.tice.2020.101479
更新日期:2021-02-01 00:00:00
abstract::The organization and ultrastructure of the accessory glands of the cattle tick, Boophilus microplus, are described. The glands consist of two groups of acinar cells situated on either side of Gene's organ. A single acinus consists of from eight to 12 cells and each cell is connected via an individual duct to pores on ...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/0040-8166(84)90033-8
更新日期:1984-01-01 00:00:00
abstract::Nonalcoholic fatty liver disease (NAFLD) is a frequent health problem. The insulin resistance and endoplasmic reticulum (ER) stress have been suggested to play important roles in the development and progression of NAFLD. However these processes and correlations have not fully been understood yet. Azoramide, an antidia...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/j.tice.2019.07.001
更新日期:2019-08-01 00:00:00
abstract::The abdominal nerve cord of adult Periplaneta americana has been maintained in culture for up to 5 weeks. The ultrastructural appearance of in vitro cords resembles that of in vivo cords although some degeneration of glia may occur during the first 2 weeks in culture. The glial cells most affected are those which make...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/0040-8166(85)90022-9
更新日期:1985-01-01 00:00:00
abstract::During the last decade a sophisticated biophysical technique called fluorescence recovery after photobleaching (FRAP) has become available for the measurement of lateral diffusion constants of lipids and proteins in the cell membrane. The information obtainable by this method is of great importance; however, the appli...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/0040-8166(88)90044-4
更新日期:1988-01-01 00:00:00
abstract::A morphological and ultrastructural study was carried out on the spermatophore and spermatodoses of some species of Orthoptera Tettigoniidae. From the results concerning the spermatophore it emerged that this structure has a morphological and ultrastructural organization represented by a dilated ampulla and a peduncle...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/s0040816602000344
更新日期:2002-10-01 00:00:00
abstract::Lenticles are dome-shaped circles or ovals of cuticle with a dark rim. They occur with a precise segmental arrangement in the larvae and pupae of lycaenid and hesperiid butterflies. In Calpodes ethlius (Lepidoptera, Hesperiidae) each lenticle is secreted by a pair of large polyploid epidermal cells. The dark rim or an...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/0040-8166(84)90048-x
更新日期:1984-01-01 00:00:00
abstract::We studied the morphology of rabbit psoas muscle fixed at increasing intervals of time in a chemical skinning solution (Wood et al., 1975), or after skinning and storage for times up to 1 week. The storage solution, in which the chemically skinned muscled fibers were kept at -20 degrees C, had the same ionic compositi...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/0040-8166(79)90062-4
更新日期:1979-01-01 00:00:00
abstract::The structure of the periostracum in the fresh-water mussel Amblema has been described using light microscopy, transmission elec;ron microscopy, and scanning electron microscopy. The structure and evolutive course of the periostracum was studied along its entire length, from the periostracal groove until it forms the ...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/0040-8166(79)90020-x
更新日期:1979-01-01 00:00:00
abstract::Degeneration of intervertebral disk (IVD) has been increased in recent years. The lumbar herniation can be cured using conservative and surgical procedures. Surgery is considered after failure of conservative treatment. Partial discectomy, fusion, and total disk replacement (TDR) are also common surgical treatments fo...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/j.tice.2014.05.008
更新日期:2014-10-01 00:00:00
abstract::Mouse metrial gland explants were cultured for periods of up to 7 days. Granulated metrial gland cells migrated from the explants onto the culture dish substratum with a peak in numbers of emigrant cells usually found after 1-3 days of culture. Histological examination of the cultured explants showed that the central ...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/0040-8166(93)90034-i
更新日期:1993-12-01 00:00:00
abstract::The transformation of the slow contracting larval m. obliquus lateralis caudalis II during metamorphosis into the asynchronous indirect flight muscle, m. obliquus lateralis dorsalis, in the Colorado beetle, Leptinotarsa decemlineata, was examined by electron microscopy. Particular attention was paid to the fate of the...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/0040-8166(86)90066-2
更新日期:1986-01-01 00:00:00
abstract::Experimental use of primary cultures of endocrine pancreas is constrained by early, vigorous proliferation of fibroblastoid cells. The addition of heavy metals, sodium ethylmercurithiosalicylate, phenyl mercuric acetate, phenyl mercuric nitrate and sodium aurothiomalate to the culture media selectively destroys these ...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/0040-8166(75)90041-5
更新日期:1975-01-01 00:00:00
abstract::Isolation and culture of Xenopus laevis neural tubes resulted in differentiation of melanophores and iridophores from neural crest cells; the differentiated melanophores and iridophores were then maintained in culture for more than 6 months. Guanosine has been reported to promote reflecting platelet formation in melan...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/j.tice.2018.08.003
更新日期:2018-10-01 00:00:00
abstract::Keratinization in the epidermis of amphibians and the lungfish has been studied by electron microscopy, autoradiography and immunocytochemistry to determine whether histidine-rich proteins, filaggrin and loricrin are present. In the lungfish and amphibian tadpoles, anti-keratin antibodies (AE1 and AE3) stain the whole...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1054/tice.2001.0198
更新日期:2001-10-01 00:00:00
abstract::Antiserum to leucokinin I, a neuropeptide originally isolated from the cockroach Leucophaea maderae, was used for immunocytochemical labeling of neurons in the brain and ventral ganglia of the moth Spodoptera litura during postembryonic development. In the ventral ganglia, leucokinin-like immunoreactivity begins to oc...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/s0040-8166(98)80008-6
更新日期:1998-02-01 00:00:00
abstract::The failure of regeneration of damaged liver in various end-stage liver diseases results in high morbidity and mortality. In this context, we have demonstrated the differentiation ability of human hematopoietic stem cells (HSCs) into hepatocytes. In this study, HSCs were isolated from a donor and cultured which exhibi...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/j.tice.2019.101312
更新日期:2020-02-01 00:00:00
abstract::Cell surface expression of the epidermal growth factor (EGF) receptor in several cell lines declines as a function of increased cell density and is associated with diminished responsiveness to EGF. However, the mechanism whereby this density-induced down regulation of receptors occurs has not been discerned. In the pr...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/0040-8166(93)90061-o
更新日期:1993-01-01 00:00:00
abstract::The existence and distribution of mammosomatotrophs (MS cells) containing growth hormone (GH) and prolactin (PRL) in bovine adenohypophysis were detailed by a combined method of mirror sections and immunohistochemical staining. MS cells always occurred in bovine adenohypophysis but their number was low. In the midsagi...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1054/tice.1999.0051
更新日期:1999-10-01 00:00:00