Abstract:
:Enzymatically dissociated hamster tracheal epithelial (HTE) cells were cultured on collagen coated Millicell filters. Within 3-5 days after being placed in culture large numbers of ciliated and mucus cells began to appear. By 1 week the HTE cells closely resembled those seen in vivo, i.e. columnar morphology and organelle polarity. After 4 weeks in vitro the HTE cultures were still able to maintain the polarity and overall columnar morphology found in in vivo tissue. There was, in addition, no apparent degradation of the collagen substrate. Auto-radiographic data indicated that there was an initial period of high DNA synthesis during the first 3 days in vitro. This was followed by a second phase in which, by day 6, the amount of DNA synthesis was greatly reduced. Analysis of the numbers of ciliated cells relative to non-ciliated cells demonstrated that between days 5 and 8 there was an increase in the percentage of ciliated cells, suggesting that cellular differentiation (i.e. ciliogenesis) follows cellular proliferation. The results of this study show that when HTE cells are grown on collagen-coated Millicell filters there is a significant improvement in cell growth and morphology yielding cells that are very similar to those present under in vivo conditions. Moreover, since there is no degradation of the collagen substrate, HTE cultures may be suitable for long-term studies of respiratory tract epithelia.
journal_name
Tissue Celljournal_title
Tissue & cellauthors
Moller PC,Partridge LR,Cox R,Pellegrini V,Ritchie DGdoi
10.1016/0040-8166(87)90019-xsubject
Has Abstractpub_date
1987-01-01 00:00:00pages
783-91issue
6eissn
0040-8166issn
1532-3072journal_volume
19pub_type
杂志文章相关文献
TISSUE & CELL文献大全abstract::Until primate sperm are exposed to the unique microenvironment of the epididymis, they are not capable of fertilization or vigorous motility. Many of the proteins that contribute to the unique microenvironment of the primate epididymis, and thus to sperm maturation, are dependent on androgens to induce their synthesis...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1054/tice.2000.0164
更新日期:2001-06-01 00:00:00
abstract::The three-dimensional aspect of rat and monkey lung multilamellar bodies was demonstrated in lipid retained thin sections. The glutaraldehyde and urea lipid retention embedment and an Epon 812 resin polar dehydrant procedure were utilized to retain lamellar lipids for precise morphological study. The unextracted multi...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/0040-8166(76)90040-9
更新日期:1976-01-01 00:00:00
abstract::The effect of the detergent digitonin on lysis of granule and plasma membranes of human neutrophils was studied. Either linear or sigmoid dose-response for release of the cytoplasmic marker lactate dehydrogenase and the granule markers lysozyme, beta-glucuronidase, lactoferrin, and myeloperoxidase was noted using digi...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/0040-8166(85)90036-9
更新日期:1985-01-01 00:00:00
abstract::Surface modification is an important step in making a synthetic polymer cytocompatible. We have previously reported improved cytocompatibility of immortalized human keratinocytes (HaCaT) with the otherwise bioinert fluorinated ethylene propylene (FEP) upon treatment with argon plasma discharge. In this article, we sho...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/j.tice.2019.05.004
更新日期:2019-06-01 00:00:00
abstract::The teleost species Notopterus notopterus (Pallas) possess bundled photoreceptors in their retina. It was found that the margin of the inner portion (the vitreal half) of photoreceptor bundles emits thin processes. Each process terminates on the contralateral photoreceptor bundle, or the processes from adjacent photor...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/j.tice.2019.03.003
更新日期:2019-06-01 00:00:00
abstract::Acute spinal cord injuries (ASCI) are common neural disorders in traumatology medicine. MicroRNA-210 (miR-210) plays a crucial role in cell survival, endothelial cell migration and cell regeneration. This paper is aim to validate the pathophysiological function of miR-210 on ASCI. We built a rat model of ASCI and util...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/j.tice.2019.02.004
更新日期:2019-04-01 00:00:00
abstract::Intercalated discs are exceptionally complex entities, and possess considerable functional significance in terms of the workings of the myocardium. Examination of different species and heart regions indicates that the original histological term has become out-moded; it is likely, however, that all such complexes will ...
journal_title:Tissue & cell
pub_type: 杂志文章,评审
doi:10.1016/0040-8166(85)90001-1
更新日期:1985-01-01 00:00:00
abstract::The fine structure of the principal and ancillary metathoracic flight muscle fibres in the adult male of a strepsipteran, Elenchus tenuicornis, is described. Power-producing dorsal longitudinal and dorso-ventral flight muscles show features consistent with myoneural asynchrony: myofibrils are large and discrete and ar...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/0040-8166(84)90072-7
更新日期:1984-01-01 00:00:00
abstract::The presence of corticotropin-releasing factor-like material in the intermaxillary glands was studied by immunocytochemical techniques during the metamorphosis of Bufo arenarum. The intermaxillary glands appeared at stage XV (midprometamorphosis) with CRF-like material slightly immunoreactive. These glands are located...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1054/tice.2000.0098
更新日期:2000-04-01 00:00:00
abstract::Little is known of the cell biology of Leydig cells during the neonatal activation of the hypothalamic-pituitary-testicular (HPT) axis. The current study examined the effect of blockade of the HPT axis with a GnRH antagonist (antide) on the neonatal population of Leydig cells in the new world primate, the common marmo...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/s0040-8166(98)80084-0
更新日期:1998-12-01 00:00:00
abstract::The typical outer coat (the yellow cuticle) disclosed in the Locusta migratoria migratorioldes egg appears to be a usual formation in Acrididae; its physiological significance is discussed. There is a diversification of the epicuticle in the anterior egg pole, very likely related to a diversified water permeability of...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/0040-8166(93)90013-b
更新日期:1993-08-01 00:00:00
abstract::Oral squamous cell carcinoma (OSCC) has contributed 90% of oral cancer worldwide. In situ histological evaluation of tissue sections is the gold standard for oral cancer detection. Formation of keratinization and keratin pearl is one of the most important histological features for OSCC grading. This paper aims at deve...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/j.tice.2015.04.009
更新日期:2015-08-01 00:00:00
abstract::We have previously shown that Elasmobranchs-characterized by a partially calcified cartilaginous endoskeleton-presented a bony vertebral arch containing osteoblasts, osteocytes and resorbing cells. The aim of this study is to test the ability of Elasmobranchs to resorb bone tissue. The subcutaneous implantation in dog...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/0040-8166(89)90043-8
更新日期:1989-01-01 00:00:00
abstract::Experiments in vitro have shown that 20-hydroxyecdysone (at a concentration of 0.2 and 2 microM and after 12 and 24 hr of incubation) is able to induce the precocious deposition of chorion materials by the follicular epithelium of young oocytes of the cockroach Blattella germanica. Since previous studies had shown tha...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/0040-8166(93)90019-h
更新日期:1993-04-01 00:00:00
abstract::The tridimensional ultrastructure of the inner cortex of rat kidney has been studied by observing freeze-fractured tissue in stereo. The complex structure of the tubules, fenestrated capillaries and glomeruli is more readily observed through the irregular fracturing of the tissue that occurs in this technique. The ult...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/0040-8166(76)90058-6
更新日期:1976-01-01 00:00:00
abstract::The inner (protein) epicuticle of the pupal cuticle of Drosophila is shown to contain at least two hydrophobic proteins (19 and 21 kD) that are also present in the outer procuticle lamellae. An N-acetylglucosamine-containing carbohydrate is also present in the inner epicuticle. This represents the first attempt to cha...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/0040-8166(86)90019-4
更新日期:1986-01-01 00:00:00
abstract::The second half of the 20th century has witnessed the birth and growth of biological ultrastructure research--a branch of cell biology in which electron microscopy plays an important role. After a humble start in around 1950, when only a limited arsenal of instrumentation was available, a wealth of auxiliary methodolo...
journal_title:Tissue & cell
pub_type: 历史文章,杂志文章,评审
doi:10.1016/j.tice.2003.11.001
更新日期:2004-04-01 00:00:00
abstract::Bovine adrenal medulla capillary endothelial cells were isolated and cloned, and their morphological behaviors in vitro were examined. In the culture of primary or early passage, one type of colony formed intracellular lumina both on the dish and in the three dimensional collagen gel. Another type proliferated well an...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/0040-8166(90)90059-i
更新日期:1990-01-01 00:00:00
abstract::Sporogony of the sporozoan Theileria parva in the salivary gland of the tick vector of East Coast fever was studied in electron micrographs. The findings differ in several respects from previous interpretations based upon light microscopy. Cytokinesis of the primary sporoblast to form secondary and tertiary sporoblast...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/0040-8166(82)90017-9
更新日期:1982-01-01 00:00:00
abstract::The sensory organs on the tarsi of the antenniform first legs of the whip spider Admetus pumilio C. L. Koch (Amblypygi, Arachnida) were examined with the scanning and transmission electron microscope. At least four different types of hair sensilla were found: (1) thick-walled bristles, which have the characteristics o...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/0040-8166(75)90009-9
更新日期:1975-01-01 00:00:00
abstract::Pituitary glands were removed from sexually mature female turtles (Chrysemys picta) and they were injected intraperitoneally (i.p.) into other mature females of the same species (experimental). In addition mature females of the same species received saline injection only (controls). Initially all the turtles used in t...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/j.tice.2006.02.001
更新日期:2006-06-01 00:00:00
abstract::The arrangement and distribution of connective tissue in six different skeletal muscles and smooth muscle was examined by scanning electron microscopy. The endomysial arrangement of collagen was similar in all types of muscle and consisted of three components: (1) myocyte-myocyte connectives; (2) myocyte-capillary con...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/0040-8166(80)90061-0
更新日期:1980-01-01 00:00:00
abstract::Routine light microscopy and transmission and scanning electron microscopy were used to describe and compare the livers of larval lampreys, Lampetra lamottenii before and during infection of the bile ducts by the nematode, Truttaedacnitis stelmioides. The hepatocytes of uninfected animals differ from other lamprey spe...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/0040-8166(92)90073-g
更新日期:1992-01-01 00:00:00
abstract::The incorporation of H(3)-histidine and H(3)-glucosamine by ovarian follicles of cecropia moths during incubation in female blood was followed autoradicgraphically. Labeling was most prominent in the follicle cells, the spaces between these cells, and the nascent yolk spheres in the oocyte cortex. Results of pulse-cha...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/s0040-8166(69)80037-6
更新日期:1969-01-01 00:00:00
abstract::We examined the participation of MAPK and PKA in the Golgi complex disassembly caused by light-activated Calphostin C in HT-29 cells. When these cells were incubated with Calphostin C, fragmentation and dispersal of the Golgi complex was observed as assessed by immunofluorescence microscopy. Electron microscopy analys...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/j.tice.2007.03.001
更新日期:2007-06-01 00:00:00
abstract::This study is designed to better understand the function of the Müller cells of the retina through the use of silicon particles that had been injected into the vitreous body of the eye and via an analysis of the number of gap junctions associated with these cells. Following intraocular injection of a silicone oil mixt...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/0040-8166(93)90028-j
更新日期:1993-01-01 00:00:00
abstract::Isolation and culture of Xenopus laevis neural tubes resulted in differentiation of melanophores and iridophores from neural crest cells; the differentiated melanophores and iridophores were then maintained in culture for more than 6 months. Guanosine has been reported to promote reflecting platelet formation in melan...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/j.tice.2018.08.003
更新日期:2018-10-01 00:00:00
abstract::Two systems of microtubules appear to be functionally significant in the transformation of a small cell into an elongated, acellular 'corkscrew', which represents the anterior region of a sperm bundle of Pseudococcus obscurus. One system of microtubules lies within the cell; it is oriented parallel to the long axis of...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/s0040-8166(71)80029-0
更新日期:1971-01-01 00:00:00
abstract::The intercellular junctions associated with the modified glial cells of the perineurium have been examined in the ganglia and main abdominal nerves of the blood-sucking bug Rhodnius prolixus, both before and and after feeding, by means of freeze-fracture and tracer studies. It was found that the pleated septate juncti...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/0040-8166(85)90040-0
更新日期:1985-01-01 00:00:00
abstract::Research spanning almost 50 years has highlighted unique characteristics and irreplaceable list of diverse functions performed by peroxisomes in various model systems. Peroxisomes are single membrane bound highly dynamic organelles ubiquitous to most eukaryotic cells. Proliferation by division of pre-existing organell...
journal_title:Tissue & cell
pub_type: 杂志文章,评审
doi:10.1016/j.tice.2017.03.002
更新日期:2017-04-01 00:00:00