Abstract：
:The second half of the 20th century has witnessed the birth and growth of biological ultrastructure research--a branch of cell biology in which electron microscopy plays an important role. After a humble start in around 1950, when only a limited arsenal of instrumentation was available, a wealth of auxiliary methodologies were developed and gradually put in use. Here we review these techniques: ultramicrotomy of "optimally" fixed and prepared samples, histochemical methods such as immuno-electron microscopy and electron microscope autoradiography, negative staining techniques, freeze-fracturing and other techniques. Closer to the millennium shift, various cryotechniques have gradually developed. Together with computer-based reconstruction methods they are likely to play increasingly more important roles in the future.
journal_name
Tissue Celljournal_title
Tissue & cellauthors
Afzelius BA,Maunsbach ABdoi
10.1016/j.tice.2003.11.001subject
Has Abstractpub_date
2004-04-01 00:00:00pages
83-94issue
2eissn
0040-8166issn
1532-3072pii
S0040816603001034journal_volume
36pub_type
历史文章,杂志文章,评审相关文献
TISSUE & CELL文献大全abstract：:Little is known of the cell biology of Leydig cells during the neonatal activation of the hypothalamic-pituitary-testicular (HPT) axis. The current study examined the effect of blockade of the HPT axis with a GnRH antagonist (antide) on the neonatal population of Leydig cells in the new world primate, the common marmo...
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1016/s0040-8166(98)80084-0
更新日期：1998-12-01 00:00:00
abstract：:Locust-style and pseudo-orthogonal structures were found in the cuticle of Heteropteryx dilatata, with the tibiae and femora containing both, and the ocular facets showing only helicoidal construction. The same epidermal cells appear to be competent to secrete both locust-style and pseudo-orthogonal cuticle structure....
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1016/0040-8166(92)90103-e
更新日期：1992-01-01 00:00:00
abstract：:In the central canal, F-actin is predominantly localized in the apical region, forming a ring-like structure around the circumference of the lumen. However, an exception is found in the medulla oblongata, where the apical F-actin becomes interrupted in the ventral aspect of the canal. To clarify the precise localizati...
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1016/j.tice.2017.01.005
更新日期：2017-04-01 00:00:00
abstract：:A detailed model for hyposmotic fluid formation in Hydra is presented. We propose that enteron fluid formation occurs in two steps: (1) segregation of an isosmotic fluid in large intercellular vacuoles with (2) subsequent reabsorption of solute in the intercellular channels to form the hyposmotic fluid of the enteron....
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1016/0040-8166(77)90045-3
更新日期：1977-01-01 00:00:00
abstract：:The oxidative agent paraquat induced tail abnormalities during Xenopus laevis development. Specimens exposed from blastula to the tadpole stage revealed pear-shaped myocytes and irregular intersomitic boundaries. The histological feature of the axial musculature was evaluated in embryos sampled at significant stages o...
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1016/j.tice.2006.03.002
更新日期：2006-06-01 00:00:00
abstract：:Brain macrophages were studied in dispersed monolayer cultures of post-natal mouse cerebella. The phagocytotic activity, binding of immunoglobulins, presence and characteristics of nucleoside diphosphatases as well as argentophilia and lipid distribution were tested, with results similar to those for brain macrophages...
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1016/0040-8166(86)90067-4
更新日期：1986-01-01 00:00:00
abstract：:Examination of livers from transgenic mice over expressing human growth hormone (hGH) revealed numerous alterations including a striking incidence of mitotic figures. The reason for increased proliferation is unclear, but could be related to effects of hGH, which is also acting as a lactogen in rodents. In order to id...
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1016/0040-8166(94)90029-9
更新日期：1994-06-01 00:00:00
abstract：:Using microsurgical manipulations, hormone applications, and transmission electron microscopy we have investigated the regulation of differentiation of the follicular epithelium and formation of the vitelline envelope (VE) in primary follicles in the ovary of the mosquito, Aedes aegypti. During the first 3 days after ...
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1016/0040-8166(91)90015-l
更新日期：1991-01-01 00:00:00
abstract：:Mature mast cells, isolated from the rat peritoneal cavity, were placed into suspension culture, either as resting or after degranulation by exposure to compound 48/80, and were maintained for up to 63 hr. No mitotic cells were observed, and cell number was conserved. The culture conditions did not cause spontaneous d...
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1016/0040-8166(82)90012-x
更新日期：1982-01-01 00:00:00
abstract：:The expression of alpha(1a)-adrenoreceptors (alpha(1a)-ARs) within the muscle spindles of rabbit masseter muscle was investigated. The alpha(1a)-ARs were detected by immunohistochemical fluorescent method and examined along the entire length of 109 cross serially sectioned spindles. The sympathetic fibers were visuali...
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1016/j.tice.2005.12.003
更新日期：2006-04-01 00:00:00
abstract：:A brief description is given of the behavioural changes in Drosophila melanogaster at the time of eclosion and wing expansion. The developmental changes in the wing during this period are classified into four stages (0-III). Details of changes in the morphology of both the cuticle and the wing epidermal cells are give...
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1016/0040-8166(87)90044-9
更新日期：1987-01-01 00:00:00
abstract：:The ultrastructure of peduncle muscle attachment to the cuticular flange in the opercular filament of the serpulid Pomatoceros lamarckii Quatrefages is described. The cuticular flange is composed of layers of orthogonally arranged fibres. Specialized epithelial cells (tendon cells) and a collagenous matrix intervene b...
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1016/0040-8166(83)90007-1
更新日期：1983-01-01 00:00:00
abstract：:The number of epithelial cells comprising larval midgut of the tobacco hornworm moth, Manduca sexta increases 200-fold in development from the first to the fifth instar. We have examined larvae periodically before and during molting to follow epithelial cell proliferation and differentiation. The midgut epithelium in ...
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1016/0040-8166(91)90058-2
更新日期：1991-01-01 00:00:00
abstract：:Isolation and culture of Xenopus laevis neural tubes resulted in differentiation of melanophores and iridophores from neural crest cells; the differentiated melanophores and iridophores were then maintained in culture for more than 6 months. Guanosine has been reported to promote reflecting platelet formation in melan...
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1016/j.tice.2018.08.003
更新日期：2018-10-01 00:00:00
abstract：:The expression and localization of gap junctional proteins connexin (Cx) 26, 32, and 43 was examined in human dental pulp. Dental pulp tissues were obtained from human third molars immediately after extraction. Some pulp tissues were used for cell culture, and the rest for histological observations. Immunostaining for...
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1016/s0040-8166(02)00028-9
更新日期：2002-06-01 00:00:00
abstract：:The large sensilla basiconica of different species of the Sphecoidea are similarly shaped. Each sensillum is associated with a large number of sense cells as well as three envelope cells, the second of which is doubled. During early development degenerating supernumerous envelope cells were observed. After the early c...
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1016/0040-8166(86)90013-3
更新日期：1986-01-01 00:00:00
abstract：:Cellular aggregates or nodules which formed in Galleria mellonella larvae in response to injections of killed Bacillus cereus were examined during their first 24 hr of development. The initial aggregation stage was very rapid, occurring within 1 min, and was brought about by the release of an adhesive flocculent mater...
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1016/0040-8166(77)90050-7
更新日期：1977-01-01 00:00:00
abstract：:The elasmoid scales of the cichlid fish, Hemichromis bimaculatus, are localized within dermal pockets, the floors of which are separated from the stratum compactum by uninterrupted cellular sheets, the scale-pocket linings (SPL). TEM study of the fry skin shows that the SPL cells originate from the cell population con...
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1016/0040-8166(89)90058-x
更新日期：1989-01-01 00:00:00
abstract：:The present study reports on the ultrastructure features of spermatozoa and spermatogenesis of several species of Dysderidae (Dysdera crocata, Dysdera erythrina, Dysdera ninnii, Harpactea arguta, Harpactea piligera, Dasumia taeniifera). Dysderid spiders are known to possess a peculiar sperm transfer form known as syns...
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1016/j.tice.2004.08.001
更新日期：2004-12-01 00:00:00
abstract：:Aromatization of androgens into estrogens in rat testis is catalyzed by the microsomal enzyme cytochrome P450 aromatase. In this work, aromatase cellular site was investigated in prepuberal, peripuberal and postpuberal testis, from 10-, 21- and 60-day-old rats respectively. Paraffin-embedded testis sections were proce...
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1054/tice.2001.0186
更新日期：2001-08-01 00:00:00
abstract：:Many insect cells, including epidermis, fat body, ocnocytcs and pericardial cells, can very easily be induced to form long fine processes or filopodia. Filopodia contain microfilaments hut differ from epidermal feet in lacking microtubules and in having a much smaller and uniform diameter. Although they may be 10-30 m...
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1016/0040-8166(87)90014-0
更新日期：1987-01-01 00:00:00
abstract：:The watery vacuoles first described from larval insect fat body (Chironomus, Voinov, 1927; Aedes, Wigglesworth, 1942; Rhodnius, Wigglesworth, 1967) have been studied in 4th and 5th stage Calpodes larvae. The vacuoles arise at the beginning (E + 6-24 hr) of the 4th stadium from plasma membrane infolds that separate fro...
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1016/0040-8166(83)90039-3
更新日期：1983-01-01 00:00:00
abstract：:The sarcoplasmic reticulum (SR) of the remarkably fast remotor muscle of lobster second antenna was investigated with regard to ultrastructure, calcium uptake and protein composition. The SR of this unique muscle dominates the volume of the cell. We were able to preserve the configuration of the SR in its native state...
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1016/0040-8166(82)90015-5
更新日期：1982-01-01 00:00:00
abstract：:The androgenic glands (AG) of male decapod crustaceans produce insulin-like androgenic gland (IAG) hormone that controls male sex differentiation, growth and behavior. Functions of the AG are inhibited by gonad-inhibiting hormone originating from X-organ-sinus gland complex in the eyestalk. The AG, and its interaction...
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1016/j.tice.2010.07.003
更新日期：2010-10-01 00:00:00
abstract：:Gap junctions are plasma membrane specializations characterized as aggregates of intramembranous particles in two apposed membranes meeting particle-to-particle in the 2-4 nm intermembrane 'gap'. Recent thin-section and freeze-fracture evidence has revealed significant structural variations of gap junctional structure...
journal_title：Tissue & cell
pub_type： 杂志文章,评审
doi：10.1016/0040-8166(77)90001-5
更新日期：1977-01-01 00:00:00
abstract：:Zebra Finches are the epitome of desert-adapted avian species; i.e. they are able to survive without drinking water for over a year. Whereas transepidermal water loss (TEWL) in naked Zebra Finch nestlings is lower than in adults, and desert adaptation is accompanied by intercellular deposition of epidermal multigranul...
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1016/0040-8166(89)90023-2
更新日期：1989-01-01 00:00:00
abstract：:Time lapse cinematography was used to study and compare the proliferation and migration activity of pulmonary endothelial cells and fibroblasts, two cell types with very different structural and functional properties. Endothelial cells were found to have a more rapid growth rate than fibroblasts. Contributing to the s...
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1016/s0040-8166(81)80002-x
更新日期：1981-01-01 00:00:00
abstract：:The spatial arrangement of tight junctions in choroid plexus and ciliary body rabbit epithelia has been determined by studying freeze-fracture complementary replicas. In the choroid plexus epithelium, the interruptions of the junctional P-face fibrils were measured to be 14% in their total length. In the ciliary body ...
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1016/0040-8166(80)90034-8
更新日期：1980-01-01 00:00:00
abstract：:The diabetes (db/db) and obese (ob/ob) genotype mutations induce a progressive, hypercytolipidemic condition within the endometrium of the female reproductive tract that promotes sterility and premature organ involution in C57BL/KsJ mice. The current studies focus on the ultrastructural changes that occur within the e...
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1016/j.tice.2003.08.002
更新日期：2004-02-01 00:00:00
abstract：:There is increasing evidence that type II alveolar cells are capable of synthesizing surface active material like that obtained from the airways. However a number of problems remain to be solved before it can be stated conclusively that type II cells synthesize the surface active material of the terminal airspace. Amo...
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1016/0040-8166(75)90028-2
更新日期：1975-01-01 00:00:00
