Golgi complex disassembly caused by light-activated calphostin C involves MAPK and PKA.

Abstract:

:We examined the participation of MAPK and PKA in the Golgi complex disassembly caused by light-activated Calphostin C in HT-29 cells. When these cells were incubated with Calphostin C, fragmentation and dispersal of the Golgi complex was observed as assessed by immunofluorescence microscopy. Electron microscopy analysis showed that clusters of vesicles and large tubule-vesicular membrane structures, resembling the Golgi remnants present in mitotic cells, substituted the Golgi stacks. In addition, Calphostin C treatment caused inhibition of the endocytic route. We confirmed that the Golgi disassembly was not due to PKC inhibition, and suggested, based on the use of specific inhibitors, that other kinases are involved. It was shown that pretreatment with PD98059 and H-89, both inhibitors of MAPK and PKA, respectively, prior to incubation with Calphostin C, caused blockade of the Golgi disassembly, as well as the inhibition of the endocytic pathway caused by this drug. This finding supports the existence of a novel mechanism by which MAPK and PKA may regulate the Golgi breakdown caused by Calphostin C in HT-29 cells.

journal_name

Tissue Cell

journal_title

Tissue & cell

authors

Morgado-Díaz JA,Montesano G,De Souza Fernandes S,Redondo PA,Fernandes de Souza W,Albuquerque-Xavier AC,Leve F,Tanaka MN,Martins de Araujo W,Oliveira SS,Benchimol M,De Souza W

doi

10.1016/j.tice.2007.03.001

subject

Has Abstract

pub_date

2007-06-01 00:00:00

pages

161-9

issue

3

eissn

0040-8166

issn

1532-3072

pii

S0040-8166(07)00022-5

journal_volume

39

pub_type

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