Abstract:
:We examined the participation of MAPK and PKA in the Golgi complex disassembly caused by light-activated Calphostin C in HT-29 cells. When these cells were incubated with Calphostin C, fragmentation and dispersal of the Golgi complex was observed as assessed by immunofluorescence microscopy. Electron microscopy analysis showed that clusters of vesicles and large tubule-vesicular membrane structures, resembling the Golgi remnants present in mitotic cells, substituted the Golgi stacks. In addition, Calphostin C treatment caused inhibition of the endocytic route. We confirmed that the Golgi disassembly was not due to PKC inhibition, and suggested, based on the use of specific inhibitors, that other kinases are involved. It was shown that pretreatment with PD98059 and H-89, both inhibitors of MAPK and PKA, respectively, prior to incubation with Calphostin C, caused blockade of the Golgi disassembly, as well as the inhibition of the endocytic pathway caused by this drug. This finding supports the existence of a novel mechanism by which MAPK and PKA may regulate the Golgi breakdown caused by Calphostin C in HT-29 cells.
journal_name
Tissue Celljournal_title
Tissue & cellauthors
Morgado-Díaz JA,Montesano G,De Souza Fernandes S,Redondo PA,Fernandes de Souza W,Albuquerque-Xavier AC,Leve F,Tanaka MN,Martins de Araujo W,Oliveira SS,Benchimol M,De Souza Wdoi
10.1016/j.tice.2007.03.001subject
Has Abstractpub_date
2007-06-01 00:00:00pages
161-9issue
3eissn
0040-8166issn
1532-3072pii
S0040-8166(07)00022-5journal_volume
39pub_type
杂志文章相关文献
TISSUE & CELL文献大全abstract::The basal plate of the scales of Amia calva is composed of regular double twisted plywood, as in Latimeria and Dipnoan scales. However, the progressive rotation of the fibrils direction is left-handed in Amia and right-handed in the 'Sarcopterygians'. So, the similarity between these peculiar plywoods is probably the ...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/0040-8166(81)90046-x
更新日期:1981-01-01 00:00:00
abstract::A panel of 40 monoclonal antibodies was constructed in response to cationic endothelial cell growth factor (c-ECGF), the cationic peptide mitogen isolated from endothelial mitogen. The monoclonal antibodies were assayed by dot blot for immunoreactivity to various other peptide angiogenic factors. The panel of monoclon...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/0040-8166(87)90040-1
更新日期:1987-01-01 00:00:00
abstract::The existence and distribution of mammosomatotrophs (MS cells) containing growth hormone (GH) and prolactin (PRL) in bovine adenohypophysis were detailed by a combined method of mirror sections and immunohistochemical staining. MS cells always occurred in bovine adenohypophysis but their number was low. In the midsagi...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1054/tice.1999.0051
更新日期:1999-10-01 00:00:00
abstract::Little is known of the cell biology of Leydig cells during the neonatal activation of the hypothalamic-pituitary-testicular (HPT) axis. The current study examined the effect of blockade of the HPT axis with a GnRH antagonist (antide) on the neonatal population of Leydig cells in the new world primate, the common marmo...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/s0040-8166(98)80084-0
更新日期:1998-12-01 00:00:00
abstract::The ultrastructure of the ovary and the female atrium during cocoon formation was investigated in the subterranean freshwater planarian Dendrocoelum constrictum. In the peripheral portion of the ovary, the oogonia are recognized as undifferentiated germ cells, which are morphologically similar to neoblasts that have a...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/j.tice.2012.09.003
更新日期:2013-02-01 00:00:00
abstract::Experiments in vitro have shown that 20-hydroxyecdysone (at a concentration of 0.2 and 2 microM and after 12 and 24 hr of incubation) is able to induce the precocious deposition of chorion materials by the follicular epithelium of young oocytes of the cockroach Blattella germanica. Since previous studies had shown tha...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/0040-8166(93)90019-h
更新日期:1993-04-01 00:00:00
abstract::Spermiogenesis of the domestic sparrow was investigated with the light and electron microscopes and a step by step classification is proposed. Three cell populations corresponding to early, mid and late spermatids were easily divided according to their positions in the seminiferous epithelium. In addition to this init...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/s0040-8166(02)00017-4
更新日期:2002-08-01 00:00:00
abstract::In the adult male rat, hemicastration (HC) induces a remarkable enhancement of testosterone secretion from the Leydig cells of the remaining testis. We have observed previously that the denervation of inferior spermatic nerves inhibits this enhancement. The present experiments were designed to assess morphometrically ...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/s0040-8166(98)80027-x
更新日期:1998-10-01 00:00:00
abstract::The spermatozoon of the Onychophoran Peripatopsis moseleyi is described. This cell is characterized by the general filiform shape, the absence of an acrosome, the presence of mitochondrial derivatives, of an annulus and of nine accessory tubules and a manchette of microtubules around the axoneme. All of these characte...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/0040-8166(76)90038-0
更新日期:1976-01-01 00:00:00
abstract::Estrogen induces oocytes development and vitellogenesis in crustacean by interacting with estrogen receptor (ER) subtypes. In the present study, we detect for the first time the ERα in oocytes and follicle cells and hepatopancreas cells of mysis by immunohistochemistry using a specific ERα antibody. ERα was mainly loc...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/j.tice.2011.12.001
更新日期:2012-04-01 00:00:00
abstract::During early-to-middle spermiogenesis in multicellular, internally fertilizing charalean green algae (Chara fibrosa, Chara vulgaris, Chara tomentosa, Nitella missouriensis), patterning of chromatin/nucleoplasm in developing spermatid nuclei changes from granules → fibers → contorted lamellae → condensed chromatin. Cyt...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/j.tice.2014.07.003
更新日期:2014-12-01 00:00:00
abstract::In the central canal, F-actin is predominantly localized in the apical region, forming a ring-like structure around the circumference of the lumen. However, an exception is found in the medulla oblongata, where the apical F-actin becomes interrupted in the ventral aspect of the canal. To clarify the precise localizati...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/j.tice.2017.01.005
更新日期:2017-04-01 00:00:00
abstract::The females of some species of the family Halophilosciidae receive in the course of mating a quantity of sperm considerably redundant with respect to the number of eggs that can be fertilized; this is possible thanks to the peculiar morpho-functional organization that characterizes their genital system and that allows...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/j.tice.2014.05.007
更新日期:2014-08-01 00:00:00
abstract::We established a human pancreatic carcinoma cell line, designated SPH, from cancerous ascites of a 57-year-old male patient with ductal adenocarcinoma of the pancreas. The cells have been cultured for 32 months with RPMI-1640 medium supplemental with 10% fetal calf serum. The population doubling time of this cell line...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/j.tice.2007.04.001
更新日期:2007-08-01 00:00:00
abstract::Mouse metrial gland explants were cultured for periods of up to 7 days. Granulated metrial gland cells migrated from the explants onto the culture dish substratum with a peak in numbers of emigrant cells usually found after 1-3 days of culture. Histological examination of the cultured explants showed that the central ...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/0040-8166(93)90034-i
更新日期:1993-12-01 00:00:00
abstract::The number of epithelial cells comprising larval midgut of the tobacco hornworm moth, Manduca sexta increases 200-fold in development from the first to the fifth instar. We have examined larvae periodically before and during molting to follow epithelial cell proliferation and differentiation. The midgut epithelium in ...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/0040-8166(91)90058-2
更新日期:1991-01-01 00:00:00
abstract::The integument of Euperipatoides leukarti (Onychophora) has been studied by SEM and TEM-histochemical techniques. The thin (2-3 mu) cuticle is essentially arthropodan in design, comprising a thin, lamellate outer epicuticle, a homogeneous inner epicuticle, and an extensive fibrous procuticle. Five tanned lipoprotein l...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/0040-8166(89)90012-8
更新日期:1989-01-01 00:00:00
abstract::Epithelial and peritubular cells associated with the reproductive tract of the snail, Limnaea stagnalis, contain an extensive system of endoplasmic reticulum that is often dilated with many closely packed intracisternal tubules. The intracisternal tubules are approximately 24-28 nm in diameter and they are often hexag...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/0040-8166(84)90059-4
更新日期:1984-01-01 00:00:00
abstract::In a previous paper, cellulose fibres were demonstrated in the larval, the metamorphosing, and the juvenile tunics. In this paper we used cytochemical methods and X-ray microanalysis to obtain additional information on tunic morphogenesis in Halocynthia papillosa. The chemical composition of the tunic evolves with its...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/s0040-8166(96)80069-3
更新日期:1996-12-01 00:00:00
abstract::Time lapse cinematography was used to study and compare the proliferation and migration activity of pulmonary endothelial cells and fibroblasts, two cell types with very different structural and functional properties. Endothelial cells were found to have a more rapid growth rate than fibroblasts. Contributing to the s...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/s0040-8166(81)80002-x
更新日期:1981-01-01 00:00:00
abstract::Examination of livers from transgenic mice over expressing human growth hormone (hGH) revealed numerous alterations including a striking incidence of mitotic figures. The reason for increased proliferation is unclear, but could be related to effects of hGH, which is also acting as a lactogen in rodents. In order to id...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/0040-8166(94)90029-9
更新日期:1994-06-01 00:00:00
abstract::The peritoneum is an extensive serous organ with both epithelial and mesenchymal features and a variety of functions. Diseases such as inflammatory peritonitis and peritoneal carcinomatosis can induce disturbance of the complex physiological functions. To understand the peritoneal response in disease, normal embryonic...
journal_title:Tissue & cell
pub_type: 杂志文章,评审
doi:10.1016/j.tice.2016.11.004
更新日期:2017-02-01 00:00:00
abstract::Bovine adrenal medulla capillary endothelial cells were isolated and cloned, and their morphological behaviors in vitro were examined. In the culture of primary or early passage, one type of colony formed intracellular lumina both on the dish and in the three dimensional collagen gel. Another type proliferated well an...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/0040-8166(90)90059-i
更新日期:1990-01-01 00:00:00
abstract::Acinar cells of extraorbital lacrimal glands from control, pilocarpine-treated, atropine-treated and atropine + pilocarpine-treated rats were studied using a potassium pyroantimonate technique and X-ray microanalysis for calcium localization at the ultrastructural level. This was done in order to identify intracellula...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/0040-8166(82)90062-3
更新日期:1982-01-01 00:00:00
abstract::Vitellogenic oocytes from the cockroach Blattella germanica were dissected free of the surrounding follicle cell layer. The continued presence of a resting potential indicated that the defolliculated oocytes remained viable. In other experiments, lysozyme was injected into last nymphal instars; scanning electron micro...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/0040-8166(90)90009-x
更新日期:1990-01-01 00:00:00
abstract::Enzymatically dissociated hamster tracheal epithelial (HTE) cells were cultured on collagen coated Millicell filters. Within 3-5 days after being placed in culture large numbers of ciliated and mucus cells began to appear. By 1 week the HTE cells closely resembled those seen in vivo, i.e. columnar morphology and organ...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/0040-8166(87)90019-x
更新日期:1987-01-01 00:00:00
abstract::The presence of stage-dependent occlusive junctions between adjacent Sertoli cells in the seminiferous epithelium of the crayfish testis was demonstrated by a lanthanum tracer study. The germinal epithelium did not appear to be compartmentalized, as evidenced by access of lanthanum to spermatogonia, spermatocytes, and...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1054/tice.2001.0211
更新日期:2001-12-01 00:00:00
abstract::We have reported that telomere fluorescence units (TFUs) of established induced pluripotent stem cells (iPSCs) derived from human amnion (hAM933) and fetal lung fibroblasts (MRC-5) were significantly longer than those of the parental cells, and that the telomere extension rates varied quite significantly among clones ...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/j.tice.2014.08.005
更新日期:2014-12-01 00:00:00
abstract::1. To be mechanically effective, supporting structures which are helicoidal need to be monodomain, with planar or concentric layers. 2. To achieve this in cholesteric liquid crystalline chemical models, a constraining surface is required. 3. The prediction which logically follows from this is that natural helicoidal s...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/0040-8166(88)90013-4
更新日期:1988-01-01 00:00:00
abstract::We used specific markers and fluorescence microscopy to identify and characterize cerebrovascular cells. Cultures were derived from brain microvessels isolated from normotensive (Wistar Kyoto, WKY) and spontaneously hypertensive (SHR) rat brains prior to, coincident with and following the onset of chronic hypertension...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/0040-8166(87)90005-x
更新日期:1987-01-01 00:00:00