Abstract：
:Chondrocytes can be isolated from human adult cartilage from metatarsal phalangeal joints. After enzymatic digestion to isolate viable cells, confluent monolayers were obtained 2-4 weeks after the start of cell division. Chondrocytes cultures, initiated and maintained in HAM's F12 with bovine fetal serum without the addition of other growth factors, produced in vitro a matrix rich in collagen and proteoglycans. Although several studies reported phenotypic instability, our results showed that the cell retain for more than 5 months in culture their differentiated characteristics, including the ability to produce cartilage-specific molecules. Chondrocyte cell lines should be useful in studying the functions of these cells from normal and abnormal tissue and for pharmacological studies in vitro.
journal_name
Tissue Celljournal_title
Tissue & cellauthors
Patti AM,Gabriele A,Della Rocca Cdoi
10.1054/tice.1999.0069subject
Has Abstractpub_date
1999-12-01 00:00:00pages
550-4issue
6eissn
0040-8166issn
1532-3072pii
S0040816699900691journal_volume
31pub_type
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