Ultrastructural and cytochemical analysis of sperm dimorphism in Drosophila subobscura.

Abstract:

:In Drosophila subobscura the male produces two classes of motile spermatozoa that differ in total length and nucleus length. The significance of this within-ejaculate polymegaly is obscure. We have carried out an ultrastructural and cytochemical analysis of both sperm morphs to understand their possible role at fertilization. Computer-aided analysis was used to clarify the complex three-dimensional structure of the spermatozoa. Short and long spermatozoa have a similar architecture. The axoneme is of the classic insect type and, together with the major mitochondrial derivative, runs for almost the whole sperm length. The axoneme ends just below the sperm apex with a centriole adjacent to the acrosome. Minor differences between the two types of sperm are related to acrosome size, nucleus morphology and relationship between nucleus and minor mitochondrial derivative. Cytophotometry of Feulgen stained samples indicated that long and short spermatozoa contain a similar amount of DNA. Both short and long spermatozoa are transferred and stored in the female upon mating. As they have similar ultrastructural and cytochemical characteristics, both sperm are potentially functional in egg penetration and karyogamy.

journal_name

Tissue Cell

journal_title

Tissue & cell

authors

Pasini ME,Redi CA,Caviglia O,Perotti ME

doi

10.1016/s0040-8166(96)80005-x

subject

Has Abstract

pub_date

1996-04-01 00:00:00

pages

165-75

issue

2

eissn

0040-8166

issn

1532-3072

pii

S0040-8166(96)80005-X

journal_volume

28

pub_type

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