Cytokine mediated proliferation of cultured sea turtle blood cells: morphologic and functional comparison to human blood cells.

Abstract:

:Blood cells from three different sea turtle species were cultured for approximately 3 weeks in nutrient medium supplemented with recombinant human cytokines known to induce terminal maturation of human hematological stem cells. Cultured turtle erythrocytes were translucent, approximately 10x larger than human erythrocytes, contained a single fluorescent inclusion body, contained nuclear epsilon (embryonic) globin proteins, and, absent of organelles while fresh cells contained few, but well defined mitochondria. Cells with basophilic cytoplasm and in all stages of proliferation were observed in cytokine-supplemented cultures and appeared to possess active protein synthesis. Cultured thrombocytes aggregated in response to agonists for at least 8 days, post-collection, contained P-selectin in the nucleus of 6 day cultured cells which appeared to be released after activation with collagen, and after 6 days had no organelles or open canalicular-like system (OCS) while freshly isolated cells demonstrated few, if any organelles but had a well developed OCS. The response of turtle cells to apparently homologous but unnatural human cytokines and the sustained biological properties of thrombocytes identify this suspension culture system as a powerful tool to explore the evolution of cell types and molecular components of hematopoiesis and hemostasis.

journal_name

Tissue Cell

journal_title

Tissue & cell

authors

Morgan DA,Class R,Violetta G,Soslau G

doi

10.1016/j.tice.2008.12.004

subject

Has Abstract

pub_date

2009-08-01 00:00:00

pages

299-309

issue

4

eissn

0040-8166

issn

1532-3072

pii

S0040-8166(09)00006-8

journal_volume

41

pub_type

杂志文章
  • Ultrastructural and cytochemical analysis of sperm dimorphism in Drosophila subobscura.

    abstract::In Drosophila subobscura the male produces two classes of motile spermatozoa that differ in total length and nucleus length. The significance of this within-ejaculate polymegaly is obscure. We have carried out an ultrastructural and cytochemical analysis of both sperm morphs to understand their possible role at fertil...

    journal_title:Tissue & cell

    pub_type: 杂志文章

    doi:10.1016/s0040-8166(96)80005-x

    authors: Pasini ME,Redi CA,Caviglia O,Perotti ME

    更新日期:1996-04-01 00:00:00

  • Depletion of immune effector cells induces myocardial damage in the acute experimental Trypanosoma cruzi infection: ultrastructural study in rats.

    abstract::The contribution of radiosensitive cells and macrophages to myocardial immunopathology has been studied in rats inoculated with Trypanosoma cruzi, Y strain. Immunodepression was induced by gamma irradiation and depletion of radioresistant macrophages was achieved by silica, a selective cytotoxic agent for macrophages....

    journal_title:Tissue & cell

    pub_type: 杂志文章

    doi:10.1054/tice.1999.0040

    authors: Melo RC

    更新日期:1999-06-01 00:00:00

  • Cytokeratin filament modulation in pulmonary microvessel endothelial cells by vasoactive agents and culture confluency.

    abstract::Recently, bovine pulmonary microvascular endothelial cells (PMV) were shown to contain cytokeratin 8 and 19 intermediate filaments (Patton et al., 1990). In this study, we examine the effect of culture contiguity and vasoactive agents on the content and assembly of cytokeratins in PMV. Immunofluorescent staining of PM...

    journal_title:Tissue & cell

    pub_type: 杂志文章

    doi:10.1016/0040-8166(91)90069-6

    authors: Alexander JS,Patton WF,Yoon MU,Shepro D

    更新日期:1991-01-01 00:00:00

  • Comparative ultrastructure of catch tentacles and feeding tentacles in the sea anemone Haliplanella.

    abstract::TEM observations of catch tentacles revealed that the tentacle tip epidermis is filled with two size classes of mature holotrich nematocysts and a gland cell filled with electron-dense vesicles. Vesicle production is restricted to upper-middle and tentacle tip regions, whereas holotrich development occurs in the lower...

    journal_title:Tissue & cell

    pub_type: 杂志文章

    doi:10.1016/0040-8166(83)90059-9

    authors: Watson GM,Mariscal RN

    更新日期:1983-01-01 00:00:00

  • A comparative analysis of deep learning architectures on high variation malaria parasite classification dataset.

    abstract::Malaria, one of the leading causes of death in underdeveloped countries, is primarily diagnosed using microscopy. Computer-aided diagnosis of malaria is a challenging task owing to the fine-grained variability in the appearance of some uninfected and infected class. In this paper, we transform a malaria parasite objec...

    journal_title:Tissue & cell

    pub_type: 杂志文章

    doi:10.1016/j.tice.2020.101473

    authors: Rahman A,Zunair H,Reme TR,Rahman MS,Mahdy MRC

    更新日期:2020-12-31 00:00:00

  • Differential distribution of glutamylated tubulin in the flagellum of mouse spermatozoa.

    abstract::Using a monoclonal antibody (GT 335) we previously demonstrated that glutamylation is a predominant posttranslational modification of alpha and beta tubulin isoforms in the axoneme of mouse spermatozoa (Fouquet et al., Cell Motil. Cytoskel. 27, 49, 1994). However, we noted that the staining intensity and/or distributi...

    journal_title:Tissue & cell

    pub_type: 杂志文章

    doi:10.1016/s0040-8166(95)80053-0

    authors: Kann ML,Prigent Y,Fouquet JP

    更新日期:1995-06-01 00:00:00

  • Electrophysiological and structural studies on the heart muscle of the lobster Homarus americanus.

    abstract::The electrophysiological properties and structure of heart muscle fibres of the lobster myocardium have been investigated. Discontinuities were observed in the expected decrement of electrotonic potentials in a study of the passive membrane properties of the muscle. Injection of Procion yellow revealed transverse barr...

    journal_title:Tissue & cell

    pub_type: 杂志文章

    doi:10.1016/s0040-8166(71)80018-6

    authors: Anderson ME,Smith DS

    更新日期:1971-01-01 00:00:00

  • Swelling of the collagen-keratocyte matrix of the bovine corneal stroma ex vivo in various solutions and its relationship to tissue thickness.

    abstract:AIM:The mammalian corneal stroma, like some other connective tissues, can absorb fluid, swell and become oedematous. Since studies on the corneal stroma have been carried out with different types of preparations and solutions, inter-study comparisons are very difficult. A study was thus undertaken on a standardised pre...

    journal_title:Tissue & cell

    pub_type: 杂志文章

    doi:10.1016/s0040-8166(00)80004-x

    authors: Doughty MJ

    更新日期:2000-12-01 00:00:00

  • Automated identification of keratinization and keratin pearl area from in situ oral histological images.

    abstract::Oral squamous cell carcinoma (OSCC) has contributed 90% of oral cancer worldwide. In situ histological evaluation of tissue sections is the gold standard for oral cancer detection. Formation of keratinization and keratin pearl is one of the most important histological features for OSCC grading. This paper aims at deve...

    journal_title:Tissue & cell

    pub_type: 杂志文章

    doi:10.1016/j.tice.2015.04.009

    authors: Das DK,Chakraborty C,Sawaimoon S,Maiti AK,Chatterjee S

    更新日期:2015-08-01 00:00:00

  • Hyposmotic fluid formation in Hydra.

    abstract::A detailed model for hyposmotic fluid formation in Hydra is presented. We propose that enteron fluid formation occurs in two steps: (1) segregation of an isosmotic fluid in large intercellular vacuoles with (2) subsequent reabsorption of solute in the intercellular channels to form the hyposmotic fluid of the enteron....

    journal_title:Tissue & cell

    pub_type: 杂志文章

    doi:10.1016/0040-8166(77)90045-3

    authors: Benos DJ,Kirk RG,Barba WP,Goldner MM

    更新日期:1977-01-01 00:00:00

  • The histophysiology and pathophysiology of the peritoneum.

    abstract::The peritoneum is an extensive serous organ with both epithelial and mesenchymal features and a variety of functions. Diseases such as inflammatory peritonitis and peritoneal carcinomatosis can induce disturbance of the complex physiological functions. To understand the peritoneal response in disease, normal embryonic...

    journal_title:Tissue & cell

    pub_type: 杂志文章,评审

    doi:10.1016/j.tice.2016.11.004

    authors: van Baal JO,Van de Vijver KK,Nieuwland R,van Noorden CJ,van Driel WJ,Sturk A,Kenter GG,Rikkert LG,Lok CA

    更新日期:2017-02-01 00:00:00

  • Ultrastructure of the putative stem cell niche in rat mammary epithelium.

    abstract::There is now strong evidence that the stem cells of many tissues reside in specialized structures termed niches. The stem cell niche functions to house and regulate symmetric and asymmetric mitosis of stem cells in mammalian skin, mouse and human bone marrow, mouse brain, gut, and hair follicle, and Drosophila ovary a...

    journal_title:Tissue & cell

    pub_type: 杂志文章

    doi:10.1016/s0040-8166(02)00107-6

    authors: Chepko G,Dickson RB

    更新日期:2003-04-01 00:00:00

  • The effect of sex hormones on lipid content and mast cell number in the harderian gland of the female toad, Bufo viridis.

    abstract::The Harderian gland of the toad Bufo viridis is a dimorphic gland owing to the presence of lipid droplets in the female glandular cells present only during summer months. Ovariectomy causes the disappearance of sudanophilia and estrogen-treatment completely prevents this change, while testosterone-injection has little...

    journal_title:Tissue & cell

    pub_type: 杂志文章

    doi:10.1016/0040-8166(94)90031-0

    authors: Minucci S,Chieffi Baccari G,Di Matteo L

    更新日期:1994-12-01 00:00:00

  • Engineering cell fate: Spotlight on cell-activation and signaling-directed lineage conversion.

    abstract::Direct reprogramming of specialized cells into other cell types has revolutionized the fields of stem cell, differentiation, and regenerative medicine. Direct reprogramming technology can convert various differentiated cell types to other fates by the forced expression of lineage-specific transcription factors. In add...

    journal_title:Tissue & cell

    pub_type: 杂志文章,评审

    doi:10.1016/j.tice.2016.07.005

    authors: Ebrahimi B

    更新日期:2016-10-01 00:00:00

  • Morphological and ultrastructural analysis of an important place of sexual communication of Rhodnius prolixus (Heteroptera: Reduviidae): the Metasternal Glands.

    abstract::Rhodnius prolixus is an important vector of Trypanosoma cruzi, the etiological agent of Chagas disease. Insect adults have a pair of Metasternal Glands (MGs) and the secretion emitted by these glands acts as sex pheromone. Recent studies have focused on the chemical composition of this pheromone, electrophysiological ...

    journal_title:Tissue & cell

    pub_type: 杂志文章

    doi:10.1016/j.tice.2020.101416

    authors: Lopes RL,Santos-Mallet JR,Barbosa CF,Gomes SAO,Spiegel CN

    更新日期:2020-12-01 00:00:00

  • Possibilities and limitations of the smearing technique for cellular studies in compact tissues: a transmission electron microscopic analysis of liver smears.

    abstract::During the last decade a sophisticated biophysical technique called fluorescence recovery after photobleaching (FRAP) has become available for the measurement of lateral diffusion constants of lipids and proteins in the cell membrane. The information obtainable by this method is of great importance; however, the appli...

    journal_title:Tissue & cell

    pub_type: 杂志文章

    doi:10.1016/0040-8166(88)90044-4

    authors: Zs-Nagy V,Zs-Nagy I,Kitani K,Ohta M,Imahori K

    更新日期:1988-01-01 00:00:00

  • Immunohistochemical localization of 2-Cys peroxiredoxins in human ciliary body.

    abstract::2-Cys peroxiredoxins (PRDX) are novel antioxidant enzymes that eliminate the hydrogen peroxide in cells to protect the cellular components from reactive oxygen species. To evaluate whether 2-Cys PRDX family plays a role in human ciliary body, the expression of PRDX I, II and III on normal human ciliary body was invest...

    journal_title:Tissue & cell

    pub_type: 杂志文章

    doi:10.1016/j.tice.2007.06.003

    authors: Hong S,Kim CY,Lee JH,Seong GJ

    更新日期:2007-10-01 00:00:00

  • Versatility of peroxisomes: An evolving concept.

    abstract::Research spanning almost 50 years has highlighted unique characteristics and irreplaceable list of diverse functions performed by peroxisomes in various model systems. Peroxisomes are single membrane bound highly dynamic organelles ubiquitous to most eukaryotic cells. Proliferation by division of pre-existing organell...

    journal_title:Tissue & cell

    pub_type: 杂志文章,评审

    doi:10.1016/j.tice.2017.03.002

    authors: Deb R,Nagotu S

    更新日期:2017-04-01 00:00:00

  • Selective isolation of rat aortic wall layers and their cell types in culture--application to converting enzyme activity measurement.

    abstract::The rat aorta, whose three wall layers can be separated by microdissection offers the rare possibility of comparing physiological characteristics of in vivo tissular cell components and corresponding cells after culture. We developed a technique allowing the dissociation of the three tunicae (intima, media and adventi...

    journal_title:Tissue & cell

    pub_type: 杂志文章

    doi:10.1016/0040-8166(94)90043-4

    authors: Battle T,Arnal JF,Challah M,Michel JB

    更新日期:1994-12-01 00:00:00

  • Fusion of myocytes with larval muscle remnants during flight muscle development in the Colorado beetle.

    abstract::The transformation of the slow contracting larval m. obliquus lateralis caudalis II during metamorphosis into the asynchronous indirect flight muscle, m. obliquus lateralis dorsalis, in the Colorado beetle, Leptinotarsa decemlineata, was examined by electron microscopy. Particular attention was paid to the fate of the...

    journal_title:Tissue & cell

    pub_type: 杂志文章

    doi:10.1016/0040-8166(86)90066-2

    authors: Smit WA,Velzing EH

    更新日期:1986-01-01 00:00:00

  • Fine structure of the retinal epithelium (RPE) of the emu (Dromaius novaehollandiae).

    abstract::As part of an ongoing comparative fine structural study, the retinal pigment epithelium (RPE), choriocapillaris and complexus basalis (Bruch's membrane) in the emu (Dromaius novaehollandiae) have been investigated by light and electron microscopy. The RPE consists of a single layer of cuboidal cells joined basally by ...

    journal_title:Tissue & cell

    pub_type: 杂志文章

    doi:10.1016/s0040-8166(98)80063-3

    authors: Braekevelt CR

    更新日期:1998-04-01 00:00:00

  • Automated oral squamous cell carcinoma identification using shape, texture and color features of whole image strips.

    abstract::Despite profound knowledge of the incidence of oral cancers and a large body of research beyond it, it continues to beat diagnosis and treatment management. Post physical observation by clinicians, a biopsy is a gold standard for accurate detection of any abnormalities. Towards the application of artificial intelligen...

    journal_title:Tissue & cell

    pub_type: 杂志文章

    doi:10.1016/j.tice.2019.101322

    authors: Rahman TY,Mahanta LB,Das AK,Sarma JD

    更新日期:2020-04-01 00:00:00

  • Unusual organization of the tropharium in the telotrophic ovarioles of an insect, Saldula saltatoria.

    abstract::The tropharium of the common shorebug Saldula saltatoria consists of 2 zones: the apical mitotic region and the distal one comprising numerous mononucleate nurse cells. Each individual nurse cell is connected to the centrally located trophic core by a thin cytoplasmic projection referred to as a trophic process. The a...

    journal_title:Tissue & cell

    pub_type: 杂志文章

    doi:10.1016/0040-8166(90)90023-3

    authors: Biliński SM,Ksiazkiewicz-Kapralska M,Stys P

    更新日期:1990-01-01 00:00:00

  • Low level laser therapy accelerates the extracellular matrix reorganization of inflamed tendon.

    abstract::In tendon lesions, inflammation indicates the beginning of tissue repair and influences cell proliferation and the remodeling of the extracellular matrix (ECM). Low level laser (LLL) therapy has been an important method to induce tissue repair, and several studies have sought to better understand the therapeutic possi...

    journal_title:Tissue & cell

    pub_type: 杂志文章

    doi:10.1016/j.tice.2017.05.006

    authors: Da Ré Guerra F,Vieira CP,Marques PP,Oliveira LP,Pimentel ER

    更新日期:2017-08-01 00:00:00

  • Dose dependent treatment with isotretinoin induces more changes in the ileum than in the duodenum and jejunum in Wistar rats.

    abstract::Acne is the most common skin disorder and can directly affect the patients' self-esteem. Systemic treatment has been indicated for nodular, cystic or persistent acne rather than another type of treatment, such as a topic one. Isotretinoin is an analogue of vitamin A and by suppressing the sebaceous glands the disease ...

    journal_title:Tissue & cell

    pub_type: 杂志文章

    doi:10.1016/j.tice.2017.03.003

    authors: Thomazini BF,Dolder MA

    更新日期:2017-04-01 00:00:00

  • Osteogenesis induced by a three-dimensional bioimplant composed of demineralised bone matrix, collagen, hydroxyapatite, and bone marrow-derived cells in massive bone defects: An experimental study.

    abstract::Treatment of massive bone defects is one of the most difficult problems to solve in orthopedics. At present, there is no consensus on the best way to resolve these problems. The aim of our study was to evaluate the effect of a three-dimensional bioimplant over massive bone defects, and to analyse if it improves the sp...

    journal_title:Tissue & cell

    pub_type: 杂志文章

    doi:10.1016/j.tice.2017.12.005

    authors: Cuervo-Lozano CE,Soto-Domínguez A,Saucedo-Cárdenas O,Montes-de-Oca-Luna R,Alonso-Romero S,Del Consuelo Mancías-Guerra M,Álvarez-Lozano E

    更新日期:2018-02-01 00:00:00

  • Selective uptake of noradrenaline, dopa, and 5-hydroxytryptamine by the nervous system of Phocanema decipiens (Nematoda): a light autoradiographic and ultrastructural study.

    abstract::As determined by light microscopic autoradiography, parts of the nervous system of Phocanema decipiens have selective and high affinity mechanisms for the uptake of tritiated noradrenaline, dihydroxyphenylalanine (dopa) and 5-hydroxytryptamine. In the nervous system, noradrenaline is accumulated only by the four papil...

    journal_title:Tissue & cell

    pub_type: 杂志文章

    doi:10.1016/0040-8166(76)90003-3

    authors: Goh SL,Davey KG

    更新日期:1976-01-01 00:00:00

  • The final stages of wing development in Drosophila melanogaster.

    abstract::A brief description is given of the behavioural changes in Drosophila melanogaster at the time of eclosion and wing expansion. The developmental changes in the wing during this period are classified into four stages (0-III). Details of changes in the morphology of both the cuticle and the wing epidermal cells are give...

    journal_title:Tissue & cell

    pub_type: 杂志文章

    doi:10.1016/0040-8166(87)90044-9

    authors: Johnson SA,Milner MJ

    更新日期:1987-01-01 00:00:00

  • Purification of monolayer cell cultures of the endocrine pancreas.

    abstract::Experimental use of primary cultures of endocrine pancreas is constrained by early, vigorous proliferation of fibroblastoid cells. The addition of heavy metals, sodium ethylmercurithiosalicylate, phenyl mercuric acetate, phenyl mercuric nitrate and sodium aurothiomalate to the culture media selectively destroys these ...

    journal_title:Tissue & cell

    pub_type: 杂志文章

    doi:10.1016/0040-8166(75)90041-5

    authors: Braaten JT,Järlfors U,Smith DS,Mintz DH

    更新日期:1975-01-01 00:00:00

  • The short spermatodesm of Arge pagana (Hymenoptera: symphyta).

    abstract::In the seminal vesicle of the 'symphyta'Arge pagana the spermatozoa are stored in motile spermatodesm bundles, maintained by an anterior cap of extracellular material. This cap consists of a denser cortex and of an internal matrix, where part of the sperm heads are embedded. The number of spermatozoa per bundle is var...

    journal_title:Tissue & cell

    pub_type: 杂志文章

    doi:10.1016/j.tice.2007.11.004

    authors: Lino-Neto J,Dolder H,Mancini K,Mercati D,Dallai R

    更新日期:2008-06-01 00:00:00