Ultrastructural localization of calcium, by electron-probe X-ray microanalysis, in the small intestine of suckling rats.

Abstract:

:The subcellular distribution of calcium has been investigated in samples, from the intestinal mucosa of 10-day rats, prepared for X-ray microanalysis by various techniques designed to minimize the loss of this element. Calcium retention and its threshold of detection was most satisfactory in freeze-dried frozen thin sections. In resin-embedded samples the best retention of calcium was found in specimens fixed in absolute ethanol, embedded without osmication, and sectioned onto glycerol. The results of this investigation indicate the presence of calcium in the supranuclear vacuole of enterocytes in the distal intestine of the neonatal rat. This calcium is probably taken up during the endocytosis of material from the intestinal lumen. The same mechanism may also be important in the uptake of other metals by suckling animals.

journal_name

Tissue Cell

journal_title

Tissue & cell

authors

Dinsdale D

doi

10.1016/0040-8166(83)90073-3

subject

Has Abstract

pub_date

1983-01-01 00:00:00

pages

417-28

issue

3

eissn

0040-8166

issn

1532-3072

pii

0040-8166(83)90073-3

journal_volume

15

pub_type

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