Gold labelling of RNA in virus-induced mitochondrial vesicles in the sheep blowfly Lucilia cuprina.

Abstract:

:Membranous vesicles are thought to be the replication site for viral RNA of many plant and animal viruses. A relatively rare site of virus-associated vesiculation is that of the mitochondrial outer membrane. In this study, virus-induced mitochondrial vesicles of the blowfly, Lucilia cuprina, were labelled with ribonuclease/gold and an antibody against double stranded RNA (anti-polyinosinic:polycytidylic acid). Both methods showed the presence of RNA in the vesicles thus indicating they may be a site for viral RNA replication in Lucilia.

journal_name

Tissue Cell

journal_title

Tissue & cell

authors

Binnington K,Brooks L

doi

10.1016/0040-8166(92)90057-e

subject

Has Abstract

pub_date

1992-01-01 00:00:00

pages

411-6

issue

3

eissn

0040-8166

issn

1532-3072

pii

0040-8166(92)90057-E

journal_volume

24

pub_type

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