Abstract:
:We examined the possibility that chondrocytes in Meckel's cartilage might secrete matrix metalloproteinase-1 (MMP-1) during degradation of the extracellular matrix. Evidence for the secretion of MMP-1 was obtained by immunohistochemical staining and immunoelectron microscopy, in addition to general histochemical staining for proteoglycans. Not only staining with toluidine blue and alcian blue but also immunostaining for chondroitin sulfate proteoglycan (CSPG) revealed that levels of glycoproteins are rapidly reduced at the late stage of degradation. MMP-1 was detected continuously in cells from chondrocytes at the early stage to hypertrophic chondrocytes at the late stage. Immunoelectron microscopy revealed that the deposition of colloidal golds shifted from an intracellular localization in chondrocytes at the early stage to pericellular spaces at the late stage. The localization of tissue inhibitor of the metalloproteinase-1 (TIMP-1) at the early stage was similar to that of MMP-1, but the level of TIMP-1 decreased significantly in hypertrophic cartilage. These findings suggest that MMP-1 is present continuously in Meckel's chondrocytes but that the active form, which degrades the extracellular matrix, is the MMP-1 that accumulates in the pericellular spaces around hypertrophic chondrocytes.
journal_name
Tissue Celljournal_title
Tissue & cellauthors
Ishizeki K,Nawa Tdoi
10.1054/tice.2000.0106subject
Has Abstractpub_date
2000-06-01 00:00:00pages
207-15issue
3eissn
0040-8166issn
1532-3072pii
S0040-8166(00)90106-Xjournal_volume
32pub_type
杂志文章相关文献
TISSUE & CELL文献大全abstract::Routine light microscopy and transmission and scanning electron microscopy were used to describe and compare the livers of larval lampreys, Lampetra lamottenii before and during infection of the bile ducts by the nematode, Truttaedacnitis stelmioides. The hepatocytes of uninfected animals differ from other lamprey spe...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/0040-8166(92)90073-g
更新日期:1992-01-01 00:00:00
abstract::Locust-style and pseudo-orthogonal structures were found in the cuticle of Heteropteryx dilatata, with the tibiae and femora containing both, and the ocular facets showing only helicoidal construction. The same epidermal cells appear to be competent to secrete both locust-style and pseudo-orthogonal cuticle structure....
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/0040-8166(92)90103-e
更新日期:1992-01-01 00:00:00
abstract::The eye muscles of the pike are organized in two distinct populations of fibers arranged in semicircular layers. The distal layer, consisting of small diameter muscle fibers, is mainly made up of type I and tonic (slow) fibers. The central and proximal (facing the eyeball) parts of the eye muscles are occupied by IIB ...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/0040-8166(91)90021-k
更新日期:1991-01-01 00:00:00
abstract::The detailed structure of the cockroach spermatheca is described and discussed firstly as an example of an insect integumentary gland, and secondly, from the standpoint of its role in reproduction. The gland comprises a cortical rank of separate secretory units, each associated with an epithelial duct cell responsible...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/s0040-8166(69)80027-3
更新日期:1969-01-01 00:00:00
abstract::Granulocytes (GRs) and/or plasmatocytes (PLs), the two major immunocytes in arthropods, participate in cellular encapsulation of foreign tissue. Although gap and desmosome junctions have been reported in insect capsules, smooth septate junctions are being reported for the first time by both thin section and freeze-fra...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/0040-8166(88)90064-x
更新日期:1988-01-01 00:00:00
abstract::Docetaxel (DCT) is an anticancer drug which acts by disrupting microtubule dynamics in the highly mitotic cancer cells. Thus, this drug has a potential to affect function and organization of tissues exhibiting high cellular turnover. We investigated, in the rabbit, the effects of a single human equivalent dose (6.26 m...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/j.tice.2014.04.005
更新日期:2014-06-01 00:00:00
abstract::The receptor activator of NF-kappa B ligand (RANKL) and its decoy receptor, osteoprotegerin (OPG), are the important proteins involved in osteoclastogenesis. In this study, we investigated the expressions of RANKL and OPG in cultured human periodontal ligament cells derived from deciduous teeth (DPDL cells) and their ...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1054/tice.2002.0223
更新日期:2002-02-01 00:00:00
abstract::Keratinization in the epidermis of amphibians and the lungfish has been studied by electron microscopy, autoradiography and immunocytochemistry to determine whether histidine-rich proteins, filaggrin and loricrin are present. In the lungfish and amphibian tadpoles, anti-keratin antibodies (AE1 and AE3) stain the whole...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1054/tice.2001.0198
更新日期:2001-10-01 00:00:00
abstract::During fixation of single muscles fibers with glutaraldehyde, the volume of the fiber shrinks 20%, recovers in rinse and osmium tetroxide to near normal volume and shrinks 20% again when staining with uranyl acetate. This suggest that osmotic properties of membranes may not have been completely lost during fixation, p...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/0040-8166(75)90013-0
更新日期:1975-01-01 00:00:00
abstract::Three basic sublayers are identified in the epicuticle of the mineralised sclerites of the Atlantic shore crab Carcinus maenas (Crustacea, Decapoda): the surface coat, the cuticulin layer, and the inner epicuticle. Their morphogenesis and subsequent changes are described throughout the moulting cycle in the normal cut...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/s0040-8166(05)80061-8
更新日期:1995-10-01 00:00:00
abstract::Until primate sperm are exposed to the unique microenvironment of the epididymis, they are not capable of fertilization or vigorous motility. Many of the proteins that contribute to the unique microenvironment of the primate epididymis, and thus to sperm maturation, are dependent on androgens to induce their synthesis...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1054/tice.2000.0164
更新日期:2001-06-01 00:00:00
abstract::The present study reports on the ultrastructure features of spermatozoa and spermatogenesis of several species of Dysderidae (Dysdera crocata, Dysdera erythrina, Dysdera ninnii, Harpactea arguta, Harpactea piligera, Dasumia taeniifera). Dysderid spiders are known to possess a peculiar sperm transfer form known as syns...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/j.tice.2004.08.001
更新日期:2004-12-01 00:00:00
abstract::The watery vacuoles first described from larval insect fat body (Chironomus, Voinov, 1927; Aedes, Wigglesworth, 1942; Rhodnius, Wigglesworth, 1967) have been studied in 4th and 5th stage Calpodes larvae. The vacuoles arise at the beginning (E + 6-24 hr) of the 4th stadium from plasma membrane infolds that separate fro...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/0040-8166(83)90039-3
更新日期:1983-01-01 00:00:00
abstract::Pelagic amphipods belonging to the genus Phronima have four compound eyes; two lateral eyes and two large transparent medial eyes which comprise the entire top of the head. The eyes are structurally similar but the crystalline cones of the medial eyes are more than twenty times as long as those of the lateral eyes, re...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/0040-8166(77)90010-6
更新日期:1977-01-01 00:00:00
abstract::Research spanning almost 50 years has highlighted unique characteristics and irreplaceable list of diverse functions performed by peroxisomes in various model systems. Peroxisomes are single membrane bound highly dynamic organelles ubiquitous to most eukaryotic cells. Proliferation by division of pre-existing organell...
journal_title:Tissue & cell
pub_type: 杂志文章,评审
doi:10.1016/j.tice.2017.03.002
更新日期:2017-04-01 00:00:00
abstract::The electrophysiological properties and structure of heart muscle fibres of the lobster myocardium have been investigated. Discontinuities were observed in the expected decrement of electrotonic potentials in a study of the passive membrane properties of the muscle. Injection of Procion yellow revealed transverse barr...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/s0040-8166(71)80018-6
更新日期:1971-01-01 00:00:00
abstract::Experimental use of primary cultures of endocrine pancreas is constrained by early, vigorous proliferation of fibroblastoid cells. The addition of heavy metals, sodium ethylmercurithiosalicylate, phenyl mercuric acetate, phenyl mercuric nitrate and sodium aurothiomalate to the culture media selectively destroys these ...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/0040-8166(75)90041-5
更新日期:1975-01-01 00:00:00
abstract::The structure of the sclerite and intersegmental cuticle of the opithosoma of the desert scorpion, Hadrurus arizonensis, has been examined by transmission electron microscopy. The sclerite cuticle contains a four-layered epicuticle, a hyaline exocuticle, an inner exocuticle and an endocuticle. The outer part of the hy...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/0040-8166(79)90040-5
更新日期:1979-01-01 00:00:00
abstract::The integument of Euperipatoides leukarti (Onychophora) has been studied by SEM and TEM-histochemical techniques. The thin (2-3 mu) cuticle is essentially arthropodan in design, comprising a thin, lamellate outer epicuticle, a homogeneous inner epicuticle, and an extensive fibrous procuticle. Five tanned lipoprotein l...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/0040-8166(89)90012-8
更新日期:1989-01-01 00:00:00
abstract::The normal cobblestone monolayer architecture of cultured vascular endothelium becomes rapidly disorganized after contact of the cell layer with a fibrin clot. The cells of a confluent endothelial monolayer separate into individual migratory cells in 4--6 hr after contact with fibrin. The effect is reversible in that ...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/0040-8166(79)90010-7
更新日期:1979-01-01 00:00:00
abstract:BACKGROUND:Mesenchymal stem cells (MSCs) are currently the most promising candidates in regenerative medicine. Nonetheless, there are several limitations associated with the MSC tissue source such as infrequent and invasive bone marrow sampling methods. To overcome these limitations, we have procured MSCs from the mens...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/j.tice.2018.09.010
更新日期:2018-12-01 00:00:00
abstract::Moist Exposed Burn Ointment (MEBO(®)) is widely used topical agent applied on skin burn. This study investigated the effect of MEBO topical application on activation and proliferation of epidermal stem cells through the immunohistochemical localization of cytokeratin 19 (CK19) as a known marker expressed in epidermal ...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/j.tice.2014.01.002
更新日期:2014-04-01 00:00:00
abstract::Rhodnius prolixus is an important vector of Trypanosoma cruzi, the etiological agent of Chagas disease. Insect adults have a pair of Metasternal Glands (MGs) and the secretion emitted by these glands acts as sex pheromone. Recent studies have focused on the chemical composition of this pheromone, electrophysiological ...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/j.tice.2020.101416
更新日期:2020-12-01 00:00:00
abstract::The localization of lactoferrin (LF) was studied in bovine exocrine glands by the peroxidase-antiperoxidase method. In general, LF immunoreactivity was demonstrated in most of the serous demilunes of the mixed glands and in some but not all acinar cells of the serous glands. LF was not detected in the acinar cells of ...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/0040-8166(93)90059-t
更新日期:1993-10-01 00:00:00
abstract::Degeneration of intervertebral disk (IVD) has been increased in recent years. The lumbar herniation can be cured using conservative and surgical procedures. Surgery is considered after failure of conservative treatment. Partial discectomy, fusion, and total disk replacement (TDR) are also common surgical treatments fo...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/j.tice.2014.05.008
更新日期:2014-10-01 00:00:00
abstract::We examined the development of serous (poison) cutaneous glands in larval and juvenile Scinax nasica (Hylidae) at the ultrastructural level. We describe the biosynthesis and maturation of the cutaneous poison in comparison with the corresponding processes in representatives of Discoglossidae, Leptodactylidae, Pelobati...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/s0040-8166(03)00049-1
更新日期:2003-08-01 00:00:00
abstract::We aimed to investigate the application of adipose-derived stromal cells in the treatment of stress urinary incontinence (SUI). Animal models of stress urinary incontinence were established with Sprague-Dawley female rats by complete cutting of the pudendal nerve. Rat adipose-derived stromal cells were isolated, cultu...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/j.tice.2011.04.003
更新日期:2011-08-01 00:00:00
abstract::The sarcoplasmic reticulum (SR) of the remarkably fast remotor muscle of lobster second antenna was investigated with regard to ultrastructure, calcium uptake and protein composition. The SR of this unique muscle dominates the volume of the cell. We were able to preserve the configuration of the SR in its native state...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/0040-8166(82)90015-5
更新日期:1982-01-01 00:00:00
abstract::Cell surface expression of the epidermal growth factor (EGF) receptor in several cell lines declines as a function of increased cell density and is associated with diminished responsiveness to EGF. However, the mechanism whereby this density-induced down regulation of receptors occurs has not been discerned. In the pr...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/0040-8166(93)90061-o
更新日期:1993-01-01 00:00:00
abstract::The surface topologies of mouse adrenal cortex tumor cells of primary or clonal origin grown as monolayer cell cultures were observed by scanning electron microscopy following their exposure to substances that effect steroid release and/or cell rounding. ACTH induced cell rounding with a concomitant profuse developmen...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/0040-8166(77)90034-9
更新日期:1977-01-01 00:00:00