Abstract：
:The receptor activator of NF-kappa B ligand (RANKL) and its decoy receptor, osteoprotegerin (OPG), are the important proteins involved in osteoclastogenesis. In this study, we investigated the expressions of RANKL and OPG in cultured human periodontal ligament cells derived from deciduous teeth (DPDL cells) and their roles in osteoclastogenesis. Northern blotting revealed that the OPG mRNA was down-regulated by application of 10(-8) M 1 alpha, 25(OH)2 vitamin D3 [1,25-(OH)2D3] and 10(-7) M dexamethasone (Dex). In contrast, RANKL mRNA was up-regulated by the same treatment. Western blotting demonstrated a decrease in OPG following application of 1, 25-(OH)2D3 and Dex. Tartrate-resistant acid phosphatase (TRAP)-positive multinuclear cells (MNCs) were induced when DPDL cells were co-cultured with mouse bone marrow cells in the presence of 1,25-(OH)2D3 and Dex. TRAP-positive MNCs increased significantly when the DPDL cells were co-cultured with bone marrow cells in the presence of anti-human OPG antibody together with 1, 25-(OH)2D3 and Dex. These results indicate that PDL cells derived from deciduous teeth synthesize both RANKL and OPG and could regulate the differentiation of osteoclasts.
journal_name
Tissue Celljournal_title
Tissue & cellauthors
Hasegawa T,Kikuiri T,Takeyama S,Yoshimura Y,Mitome M,Oguchi H,Shirakawa Tdoi
10.1054/tice.2002.0223subject
Has Abstractpub_date
2002-02-01 00:00:00pages
44-51issue
1eissn
0040-8166issn
1532-3072pii
S0040816602902235journal_volume
34pub_type
杂志文章相关文献
TISSUE & CELL文献大全abstract：:Gap junctions are plasma membrane specializations characterized as aggregates of intramembranous particles in two apposed membranes meeting particle-to-particle in the 2-4 nm intermembrane 'gap'. Recent thin-section and freeze-fracture evidence has revealed significant structural variations of gap junctional structure...
journal_title：Tissue & cell
pub_type： 杂志文章,评审
doi：10.1016/0040-8166(77)90001-5
更新日期：1977-01-01 00:00:00
abstract：:Mature mast cells, isolated from the rat peritoneal cavity, were placed into suspension culture, either as resting or after degranulation by exposure to compound 48/80, and were maintained for up to 63 hr. No mitotic cells were observed, and cell number was conserved. The culture conditions did not cause spontaneous d...
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1016/0040-8166(82)90012-x
更新日期：1982-01-01 00:00:00
abstract：:The expression of alpha(1a)-adrenoreceptors (alpha(1a)-ARs) within the muscle spindles of rabbit masseter muscle was investigated. The alpha(1a)-ARs were detected by immunohistochemical fluorescent method and examined along the entire length of 109 cross serially sectioned spindles. The sympathetic fibers were visuali...
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1016/j.tice.2005.12.003
更新日期：2006-04-01 00:00:00
abstract：:Isolation and culture of Xenopus laevis neural tubes resulted in differentiation of melanophores and iridophores from neural crest cells; the differentiated melanophores and iridophores were then maintained in culture for more than 6 months. Guanosine has been reported to promote reflecting platelet formation in melan...
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1016/j.tice.2018.08.003
更新日期：2018-10-01 00:00:00
abstract：:The eye muscles of the pike are organized in two distinct populations of fibers arranged in semicircular layers. The distal layer, consisting of small diameter muscle fibers, is mainly made up of type I and tonic (slow) fibers. The central and proximal (facing the eyeball) parts of the eye muscles are occupied by IIB ...
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1016/0040-8166(91)90021-k
更新日期：1991-01-01 00:00:00
abstract：:The tropharium of the common shorebug Saldula saltatoria consists of 2 zones: the apical mitotic region and the distal one comprising numerous mononucleate nurse cells. Each individual nurse cell is connected to the centrally located trophic core by a thin cytoplasmic projection referred to as a trophic process. The a...
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1016/0040-8166(90)90023-3
更新日期：1990-01-01 00:00:00
abstract：:The albumen gland of Pomacea paludosa, a prosobranch gastropod, contains two main ducts. The albumen gland duct consists of a single layer of secretory and non-secretory cells. The surface of the non-secretory cells is covered with cilia. Microvilli are associated with the luminal edges of the secretory cells. Globule...
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1016/0040-8166(84)90022-3
更新日期：1984-01-01 00:00:00
abstract：:The Malpighian tubules of adult flesh fly, Sarcophaga ruficornis consist of principal and stellate cells. The principal cells reveal all the characteristics of transporting epithelia with well developed deep basal membrane infoldings forming a complex of interconnecting labyrinth of canaliculi and luminal microvilli, ...
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1016/j.tice.2013.04.002
更新日期：2013-10-01 00:00:00
abstract：:The loss of oligodendrocyte progenitor cells (OPC) is a hallmark of perinatal brain injury. Our aim was to develop an in vitro culture condition for human chorion-derived mesenchymal stem cells (MSC) that enhances their stem cell properties and their capability to differentiate towards OPC-like cells. MSC were grown e...
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1016/j.tice.2018.04.002
更新日期：2018-06-01 00:00:00
abstract：:The present study reports on the ultrastructure features of spermatozoa and spermatogenesis of several species of Dysderidae (Dysdera crocata, Dysdera erythrina, Dysdera ninnii, Harpactea arguta, Harpactea piligera, Dasumia taeniifera). Dysderid spiders are known to possess a peculiar sperm transfer form known as syns...
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1016/j.tice.2004.08.001
更新日期：2004-12-01 00:00:00
abstract：:Polychlorinated biphenyls (PCBs) are worldwide pollutants and have caused hazardous effects on many animal species including humans. They have been detected in human milk and therefore exposure of newborns to PCBs is unavoidable if they are breast-fed. We present our findings on two experiments performed to test the e...
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1054/tice.2000.0168
更新日期：2001-04-01 00:00:00
abstract：:The origin of midgut epithelium may begin either from yolk cells (energids), tips of stomo- and proctodaeum (ectoderm), inner layer (endoderm) or from both kinds of the above mentioned cells. The origin of the midgut epithelium in wingless insects (Apterygota) has still not been determined. In Thermobia domestica the ...
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1016/j.tice.2004.12.001
更新日期：2005-04-01 00:00:00
abstract：:Fluorescent staining of Rhodnius prolixus ovarioles with rhodaminylphalloidin revealed an elaborate interconnecting meshwork of F-actin encasing the microtubule rich core. The thick actin struts have branches extending partially into the nurse cell lobes extending from the syncytical core. Basally the network ends at ...
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1016/0040-8166(86)90075-3
更新日期：1986-01-01 00:00:00
abstract：:Sepsis was a systemic response to a local infection. Apoptosis was observed in the experimental sepsis. In this study, cecal ligation and puncture (CLP)-induced sepsis was established in rats. We found that sepsis decreased thyroid hormone levels, including triiodothyronine (T3), thyroxine (T4), free T3 (fT3), and fre...
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1016/j.tice.2016.07.001
更新日期：2016-10-01 00:00:00
abstract：:Mouse metrial gland explants were cultured for periods of up to 7 days. Granulated metrial gland cells migrated from the explants onto the culture dish substratum with a peak in numbers of emigrant cells usually found after 1-3 days of culture. Histological examination of the cultured explants showed that the central ...
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1016/0040-8166(93)90034-i
更新日期：1993-12-01 00:00:00
abstract：:Intratubular injection of electron-opaque tracers (lanthanum hydroxide, peroxidase) by micromanipulation showed that the intercellular spaces of the adluminal compartment are in continuity with the lumen of the rat seminiferous tubule at all stages of the spermatogenic cycle. This continuity involves the intercellular...
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1016/0040-8166(88)90059-6
更新日期：1988-01-01 00:00:00
abstract：:Reptilian epidermis contains two types of keratin, soft (alpha) and hard (beta). The biosynthesis and molecular weight of beta-keratin during differentiation of lizard epidermis have been studied by autoradiography, immunocytochemistry and immunoblotting. Tritiated proline is mainly incorporated into differentiating a...
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1016/j.tice.2004.06.001
更新日期：2004-10-01 00:00:00
abstract：:Antiserum to leucokinin I, a neuropeptide originally isolated from the cockroach Leucophaea maderae, was used for immunocytochemical labeling of neurons in the brain and ventral ganglia of the moth Spodoptera litura during postembryonic development. In the ventral ganglia, leucokinin-like immunoreactivity begins to oc...
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1016/s0040-8166(98)80008-6
更新日期：1998-02-01 00:00:00
abstract：:The promise(s) of using Fetal Calf Serum (FCS) as a supplement for the maintenance of cell cultures has been well documented. However, FCS forms the xenogenic source for any human derived cells/organ and limits its application. Recently, the usage of human umbilical cord blood serum (hUCBS) for maintenance of mesenchy...
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1016/j.tice.2011.03.007
更新日期：2011-08-01 00:00:00
abstract：:Mammary epithelial organoids consisting of groups of lobular-alveolar acini were prepared from mid-pregnant mice and cultured for 24, 48, 96 and 192 hr on attached collagen gels in the presence of combinations of insulin, hydrocortisone and prolactin. The organoids rapidly attached to the gels and with all the combina...
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1016/0040-8166(92)90094-n
更新日期：1992-01-01 00:00:00
abstract：:A hydrogenosome is a hydrogen-producing organelle, evolutionary related to mitochondria and is found in Parabasalia protozoa, certain chytrid fungi and certain ciliates. It displays similarities to and differences from mitochondria. Hydrogenosomes are spherical or slightly elongated organelles, although very elongated...
journal_title：Tissue & cell
pub_type： 杂志文章,评审
doi：10.1016/j.tice.2009.01.001
更新日期：2009-06-01 00:00:00
abstract：:On dark-adaptation of the 11-day adult eye, the rhabdomers move towards the cuticle. Each c-c-r array (of tandem centrioles and ciliary root) maintains its position relative to the rhabdomere, and the length of the root is apparently unchanged. The close association of the array with the rhabdomere is not established ...
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1016/0040-8166(75)90038-5
更新日期：1975-01-01 00:00:00
abstract：:Specific biomechanical characters and some structures possibly related to them were investigated in the skin of the toad Bufo marinus using tensile testing techniques (at constant strain till rupture) as well as morphological methods (histological, immunohistochemical and electronmicroscopical). Mechanical parameters ...
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1054/tice.2001.0208
更新日期：2001-10-01 00:00:00
abstract：:Oligoryzomys flavescens, a common rodent of the temperate regions of South America, has been identified as a Hantavirus reservoir. There is still little information concerning its reproductive biology, which is essential to devise effective control measurement of natural populations. This rodent is a seasonal breeder ...
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1016/j.tice.2007.05.001
更新日期：2007-08-01 00:00:00
abstract：:Due to the recent importance assumed by the apoptotic process as a tool in the therapeutic protocols, we investigated the onset and rate of drug-induced apoptosis in a cell model of prostatic tumor: the DU-145 cells. The human prostatic cancer cells, DU-145, have been treated with pro-apoptotic substances like puromyc...
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1016/j.tice.2005.06.006
更新日期：2005-10-01 00:00:00
abstract：:Lenticles are dome-shaped circles or ovals of cuticle with a dark rim. They occur with a precise segmental arrangement in the larvae and pupae of lycaenid and hesperiid butterflies. In Calpodes ethlius (Lepidoptera, Hesperiidae) each lenticle is secreted by a pair of large polyploid epidermal cells. The dark rim or an...
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1016/0040-8166(84)90048-x
更新日期：1984-01-01 00:00:00
abstract：:The epithelium of the gastric mucosa and its glands in the corpus of rat stomach contains mucous surface cells (MSCs), parietal cells, mucous neck cells (MNCs), zymogenic or chief cells (ZCs), several types of enteroendocrine cells, and intermediate cells with characteristics between MNCs and ZCs also called transitio...
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1016/j.tice.2017.10.007
更新日期：2017-12-01 00:00:00
abstract：:The abdominal nerve cord of adult Periplaneta americana has been maintained in culture for up to 5 weeks. The ultrastructural appearance of in vitro cords resembles that of in vivo cords although some degeneration of glia may occur during the first 2 weeks in culture. The glial cells most affected are those which make...
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1016/0040-8166(85)90022-9
更新日期：1985-01-01 00:00:00
abstract：:The oocyte cortex undergoes dramatic changes during oogenesis in Rhodnius prolixus. Despite numerous studies examining oogenesis in the telotrophic ovariole, none has investigated the ultrastructural details of the oocyte cortex, in particular, the lateral cortical cytoskeleton. Indirect immunofluorescent staining of ...
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1016/0040-8166(93)90081-u
更新日期：1993-06-01 00:00:00
abstract：:The organization and ultrastructure of the accessory glands of the cattle tick, Boophilus microplus, are described. The glands consist of two groups of acinar cells situated on either side of Gene's organ. A single acinus consists of from eight to 12 cells and each cell is connected via an individual duct to pores on ...
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1016/0040-8166(84)90033-8
更新日期：1984-01-01 00:00:00
