Abstract：
:Due to the recent importance assumed by the apoptotic process as a tool in the therapeutic protocols, we investigated the onset and rate of drug-induced apoptosis in a cell model of prostatic tumor: the DU-145 cells. The human prostatic cancer cells, DU-145, have been treated with pro-apoptotic substances like puromycin (1 microg/ml for 4 and 6 h) and oxygen peroxide (1 mM for 1 h) or exposed to hyperthermic treatment (43 degrees C for 1 h followed by 2 h of recovery). The conventional techniques of preparation and observation of biological samples for electron scanning microscopy were used to estimate the sensitivity of these cells to the pro-apoptotic treatments and to establish their ability to undergo apoptosis. The ultrastructural changes occurring in the different phases of the phenomenon were characterized in parallel with the evaluation of the rate of apoptosis and in parallel with the evaluation of the expression levels of hsp-70 proteins.
journal_name
Tissue Celljournal_title
Tissue & cellauthors
Dini Ldoi
10.1016/j.tice.2005.06.006subject
Has Abstractpub_date
2005-10-01 00:00:00pages
379-84issue
5eissn
0040-8166issn
1532-3072pii
S0040-8166(05)00060-1journal_volume
37pub_type
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