Abstract:
:Myosin as well as actin filaments could be seen in negatively stained preparations of fresh chicken gizzard homogenized in buffered KCI (I=0.12, pH 6.85), in a 1 : 1 ratio. Myosin filaments were also present when homogenates were diluted (1 : 9) with solutions containing additional Mg(++) and ATP provided naturally occurring traces of Ca(++) had not been chelated with EGTA. There were no myosin filaments when Mg(++), ATP, Ca(++) or Ca(++) +ATP were added respectively to the diluent. It is suggested that in vivo in relaxed muscles myosin is present in dimers, and only aggregates into filaments at the onset of contraction when Ca(++) are released.
journal_name
Tissue Celljournal_title
Tissue & cellauthors
Shoenberg CFdoi
10.1016/s0040-8166(69)80007-8subject
Has Abstractpub_date
1969-01-01 00:00:00pages
83-96issue
1eissn
0040-8166issn
1532-3072pii
S0040-8166(69)80007-8journal_volume
1pub_type
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