Abstract:
:Rhodnius prolixus is an important vector of Trypanosoma cruzi, the etiological agent of Chagas disease. Insect adults have a pair of Metasternal Glands (MGs) and the secretion emitted by these glands acts as sex pheromone. Recent studies have focused on the chemical composition of this pheromone, electrophysiological responses to MGs compounds and mating behavior assays. Morphological studies of these glands are still scarce. Thus, considering the relevance of MGs in the sex pheromone biosynthesis, we investigated the morphology and ultrastructure of R. prolixus MGs. The glandular apparatus presents a tubular structure containing secretory cells with canalicules that fuse with the central duct which conducts the secretion to a pear-shaped reservoir connected to the exterior by a droplet-shape orifice. The secretory cells are classified as class III, they present a well-developed rough and smooth endoplasmic reticulum. Smooth endoplasmic reticulum is a site of lipid biosynthesis that may be involved in the mevalonate pathway, a probable route of the sex pheromone biosynthesis in this insect. The presence of rough endoplasmic reticulum indicates a possible peptides/proteins secretions site which were still not characterized in MGs. Several mitochondria are scattered in the cytoplasm that may suggest a high metabolic activity. Further studies should be carried out to correlate these data with the sex pheromone biosynthesis in this vector.
journal_name
Tissue Celljournal_title
Tissue & cellauthors
Lopes RL,Santos-Mallet JR,Barbosa CF,Gomes SAO,Spiegel CNdoi
10.1016/j.tice.2020.101416subject
Has Abstractpub_date
2020-12-01 00:00:00pages
101416eissn
0040-8166issn
1532-3072pii
S0040-8166(20)30061-6journal_volume
67pub_type
杂志文章相关文献
TISSUE & CELL文献大全abstract::The loci of calcium distribution in Nereis pharyngeal visceral muscle have been examined by cytochemical precipitation using potassium pyroantimonate. In Na-, Ca- and Mg-free media, pyroantimonate incubation was used to pinpoint loci of intracellularly bound calcium. This method also revealed heavy deposition on the i...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/0040-8166(81)90024-0
更新日期:1981-01-01 00:00:00
abstract::The fine structure of trophoblast giant cells and their interaction with collagen at the antimesometrial region on the 9th day of pregnancy was examined in fed and acute fasted mice. Collagen fibrils and filamentous aggregates (disintegrating collagen fibrils) were observed in the extracellular space. Three types of i...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/s0040-8166(05)80026-6
更新日期:1995-12-01 00:00:00
abstract::During fixation of single muscles fibers with glutaraldehyde, the volume of the fiber shrinks 20%, recovers in rinse and osmium tetroxide to near normal volume and shrinks 20% again when staining with uranyl acetate. This suggest that osmotic properties of membranes may not have been completely lost during fixation, p...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/0040-8166(75)90013-0
更新日期:1975-01-01 00:00:00
abstract::As determined by light microscopic autoradiography, parts of the nervous system of Phocanema decipiens have selective and high affinity mechanisms for the uptake of tritiated noradrenaline, dihydroxyphenylalanine (dopa) and 5-hydroxytryptamine. In the nervous system, noradrenaline is accumulated only by the four papil...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/0040-8166(76)90003-3
更新日期:1976-01-01 00:00:00
abstract::Junctional complexes between the epithelial cells in the four distinct regions of the glow-worm Malpighian tubule were investigated by electron microscopy using thin sectioning, freeze-fracturing, osmotic disruption and tracer techniques. The lateral plasma membranes of all four cell types are joined by smooth septate...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/0040-8166(80)90011-7
更新日期:1980-01-01 00:00:00
abstract::A detailed model for hyposmotic fluid formation in Hydra is presented. We propose that enteron fluid formation occurs in two steps: (1) segregation of an isosmotic fluid in large intercellular vacuoles with (2) subsequent reabsorption of solute in the intercellular channels to form the hyposmotic fluid of the enteron....
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/0040-8166(77)90045-3
更新日期:1977-01-01 00:00:00
abstract::The skin of hairless descendants of Mexican hairless dogs was investigated lectin-histochemically. Haired dogs were also used for comparative study. In the epidermis of infant pups, lectin staining revealed marked differences between hairless and haired dogs. Griffonia simplicifolia-I (GS-I) bound moderately to the cy...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/0040-8166(94)90080-9
更新日期:1994-02-01 00:00:00
abstract::Standard procedures for isolating nucleic acids from specialized tissues such as the mucus-containing tissues found in many marine organisms are, in many cases, not effective, resulting in isolates contaminated with polysaccharides that encumber subsequent analysis. A method is described for isolating nucleic acids fr...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/0040-8166(94)90013-2
更新日期:1994-08-01 00:00:00
abstract::Autologous fibrin has been widely used in surgical procedures for both soft and hard tissue repair. There are different protocols and devices to obtain this matrix, with varying centrifugal time, gravity force, speed, angle of the sample tube and spinning radius. The aim of this study was to compare three methods of o...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/j.tice.2020.101407
更新日期:2020-12-01 00:00:00
abstract::The paired ovaries of young larva of the 3rd instar of Orthezia urticae are filled with numerous germ cell clusters that can be regarded as ovariole anlagen. Germ cells (cystocytes) belonging to one cluster form a rosette, in the centre of which a polyfusome occurs. Staining with rhodamine-phalloidin has revealed that...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/s0040-8166(97)80069-9
更新日期:1997-02-01 00:00:00
abstract::The loss of oligodendrocyte progenitor cells (OPC) is a hallmark of perinatal brain injury. Our aim was to develop an in vitro culture condition for human chorion-derived mesenchymal stem cells (MSC) that enhances their stem cell properties and their capability to differentiate towards OPC-like cells. MSC were grown e...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/j.tice.2018.04.002
更新日期:2018-06-01 00:00:00
abstract:OBJECTIVE:The current study proposes an automated machine learning approach for the quantification of cells in cell death pathways according to DNA fragmentation. METHODS:A total of 17 images of kidney histological slide samples from male Wistar rats were used. The slides were photographed using an Axio Zeiss Vert.A1 ...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/j.tice.2016.12.006
更新日期:2017-02-01 00:00:00
abstract::The contribution of radiosensitive cells and macrophages to myocardial immunopathology has been studied in rats inoculated with Trypanosoma cruzi, Y strain. Immunodepression was induced by gamma irradiation and depletion of radioresistant macrophages was achieved by silica, a selective cytotoxic agent for macrophages....
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1054/tice.1999.0040
更新日期:1999-06-01 00:00:00
abstract::As already reported, epixenosomes have a complex cell organization although they lack membranous organelles and a nuclear envelope. Different morphological compartments have been evidenced: (1) a space (IM) between the 2 external membranes in which acid phosphatase has been observed; (2) an apical, electrondense zone ...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/s0040-8166(96)80018-8
更新日期:1996-06-01 00:00:00
abstract::Treatment of massive bone defects is one of the most difficult problems to solve in orthopedics. At present, there is no consensus on the best way to resolve these problems. The aim of our study was to evaluate the effect of a three-dimensional bioimplant over massive bone defects, and to analyse if it improves the sp...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/j.tice.2017.12.005
更新日期:2018-02-01 00:00:00
abstract::The ultrastructural localization of two types of biochemically characterized phenol oxidase activity is described in the larva of the sheep blowfly, Lucilia cuprina. Cuticular tyrosinase activity (enzyme A) is seen in epicuticular filaments and procuticle. Procuticle activity can be detected only after a presumed proc...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/0040-8166(88)90073-0
更新日期:1988-01-01 00:00:00
abstract::Two systems of microtubules appear to be functionally significant in the transformation of a small cell into an elongated, acellular 'corkscrew', which represents the anterior region of a sperm bundle of Pseudococcus obscurus. One system of microtubules lies within the cell; it is oriented parallel to the long axis of...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/s0040-8166(71)80029-0
更新日期:1971-01-01 00:00:00
abstract::The typical outer coat (the yellow cuticle) disclosed in the Locusta migratoria migratorioldes egg appears to be a usual formation in Acrididae; its physiological significance is discussed. There is a diversification of the epicuticle in the anterior egg pole, very likely related to a diversified water permeability of...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/0040-8166(93)90013-b
更新日期:1993-08-01 00:00:00
abstract::Inflammatory bowel disease (IBD) is a complex immunological disorder characterized by chronic inflammation caused mainly by unknown factors. The interleukin-10 knockout (IL-10 KO) mouse is a well-established murine model of IBD which develops spontaneous intestinal inflammation that resembles Crohn's disease. In the p...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/j.tice.2014.12.001
更新日期:2015-02-01 00:00:00
abstract::Enzymatically dissociated hamster tracheal epithelial (HTE) cells were cultured on collagen coated Millicell filters. Within 3-5 days after being placed in culture large numbers of ciliated and mucus cells began to appear. By 1 week the HTE cells closely resembled those seen in vivo, i.e. columnar morphology and organ...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/0040-8166(87)90019-x
更新日期:1987-01-01 00:00:00
abstract::Human amniotic mesenchymal stromal cells (hAMSCs) are considered as a population of multipotent cells. The molecular events associated with mesenchymal stromal cell (MSC)/tumor cell interactions should be studied to identify the role of MSCs on suppressing or inducing the key signaling pathways of tumor cells. Thus, d...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/j.tice.2020.101479
更新日期:2021-02-01 00:00:00
abstract::The presence of corticotropin-releasing factor-like material in the intermaxillary glands was studied by immunocytochemical techniques during the metamorphosis of Bufo arenarum. The intermaxillary glands appeared at stage XV (midprometamorphosis) with CRF-like material slightly immunoreactive. These glands are located...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1054/tice.2000.0098
更新日期:2000-04-01 00:00:00
abstract::The fine structure of the principal and ancillary metathoracic flight muscle fibres in the adult male of a strepsipteran, Elenchus tenuicornis, is described. Power-producing dorsal longitudinal and dorso-ventral flight muscles show features consistent with myoneural asynchrony: myofibrils are large and discrete and ar...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/0040-8166(84)90072-7
更新日期:1984-01-01 00:00:00
abstract::We examined the participation of MAPK and PKA in the Golgi complex disassembly caused by light-activated Calphostin C in HT-29 cells. When these cells were incubated with Calphostin C, fragmentation and dispersal of the Golgi complex was observed as assessed by immunofluorescence microscopy. Electron microscopy analys...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/j.tice.2007.03.001
更新日期:2007-06-01 00:00:00
abstract::The oocyte cortex undergoes dramatic changes during oogenesis in Rhodnius prolixus. Despite numerous studies examining oogenesis in the telotrophic ovariole, none has investigated the ultrastructural details of the oocyte cortex, in particular, the lateral cortical cytoskeleton. Indirect immunofluorescent staining of ...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/0040-8166(93)90081-u
更新日期:1993-06-01 00:00:00
abstract::Specific biomechanical characters and some structures possibly related to them were investigated in the skin of the toad Bufo marinus using tensile testing techniques (at constant strain till rupture) as well as morphological methods (histological, immunohistochemical and electronmicroscopical). Mechanical parameters ...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1054/tice.2001.0208
更新日期:2001-10-01 00:00:00
abstract::The gills of Penaeus aztecus were examined by light and electron microscopy. They are dendrobranchiate, consisting of a central axis with biserially arranged branches that subdivide into bifurcating filaments. A septum divides the lumina of these structures into afferent and efferent channels. Hemolymph from the stern...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/0040-8166(78)90008-3
更新日期:1978-01-01 00:00:00
abstract::The ova of Sicyonia ingentis lack cortical vesicles at the time of spawning. Within 30 min post-spawning, two populations of cortical vesicles are organized in the ooplasm which, during cortical vesicle exocytosis (cortical reaction), successively release two morphologically different exudates. The first type of corti...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/0040-8166(88)90035-3
更新日期:1988-01-01 00:00:00
abstract::Mammary epithelial organoids consisting of groups of lobular-alveolar acini were prepared from mid-pregnant mice and cultured for 24, 48, 96 and 192 hr on attached collagen gels in the presence of combinations of insulin, hydrocortisone and prolactin. The organoids rapidly attached to the gels and with all the combina...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/0040-8166(92)90094-n
更新日期:1992-01-01 00:00:00
abstract::The distribution of calcified microdeposits in non-atherosclerotic intima of the human aorta was studied by electron microscopy. Aortic specimens were obtained during aortic reconstruction and were embedded in Lowicryl resin. Non-stained ultrathin sections were analysed using an electron microscope equipped with an en...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/s0040-8166(98)80052-9
更新日期:1998-06-01 00:00:00