Immunocytochemical study of tubulin in the 9 + '1' sperm axoneme of a monogenean (Platyhelminthes), Pseudodactylogyrus sp.

Abstract:

:The spermatozoon of the monopisthocotylean monogenean Pseudodactylogyrus sp. (a gill parasite of eels) has a single axoneme showing a 9 + '1' pattern, a nucleus and a mitochondrion, but has no cortical microtubules. This species thus provides a very simple model for the study of tubulin in the 9 + '1' axonemes of the Platyhelminthes, in contrast with digenean sperm which have a more complex spermatozoon with two such axonemes and cortical microtubules. Indirect immunofluorescence labelling of tubulin shows that the elongating spermatids, initially lying in all directions in the early stages, are arranged as parallel elements in further stages. The number of spermatids in an isogenic group could also be precisely counted and equals 32. Nuclear labelling with fluorescent dyes shows that the nuclei, first located in the common mass of the spermatids, later elongate and migrate into the growing spermatids, and that the nucleus is located in the central part of the mature spermatozoon, with the two extremities devoid of nucleus. Labelling with antibodies directed against acetylated, tyrosinated, and polyglutamylated tubulin gave positive results, thus indicating that these post-translational modifications of tubulin are present in the axoneme of spermatids and spermatozoa of monopisthocotylean monogeneans.

journal_name

Tissue Cell

journal_title

Tissue & cell

authors

Mollaret I,Justine JL

doi

10.1016/s0040-8166(97)80045-6

subject

Has Abstract

pub_date

1997-12-01 00:00:00

pages

699-706

issue

6

eissn

0040-8166

issn

1532-3072

pii

S0040-8166(97)80045-6

journal_volume

29

pub_type

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