Abstract：
:Using [(3)H]-serotonin ([(3)H]-5-HT) as radiolabel and autoradiography, we have mapped the distribution of 5-HT uptake and binding sites in the sea pansy Renilla koellikeri in order to identify potential cellular pathways of 5-HT inactivation and to identify cellular substrates for the previously characterized 5-HT receptor involved in the modulation of peristaltic behavior. Uptake measured in fresh polyp tissues occurred via two processes: a high affinity (uptake(1)), clomipramine-sensitive process with a K(m) of 0.45 microM, and another of lower affinity (uptake(2)) with a Km of 11.6 microM. Autoradiograms of high affinity uptake sites revealed a diffuse distribution of label with higher density in the ectoderm and endoderm, and lower density in the mesoglea. No subsets of cells, including serotonergic neurons, appeared to retain label preferentially, thus suggesting that removal of 5-HT and its chemical conversion is a general property of sea pansy tissues. Under incubation conditions identical to those used in a previous radiobinding analysis, autoradiograms of binding sites were generated on sections from lightly fixed and cryosectioned polyps. In contrast to uptake sites, binding sites appeared as aggregations of label around neurons of the subectodermal, mesogleal and endodermal nerve nets. In the endoderm where the myoepithelia subtend peristalsis, myoepithelial cells appeared unlabeled, suggesting that 5-HT exerts its modulatory effect on peristalsis principally via neurons. Taken together, these results indicate that 5-HT is released as a neurohormone in the sea pansy and that it may act as a broad-range neuromodulator.
journal_name
Tissue Celljournal_title
Tissue & cellauthors
Dergham P,Anctil Mdoi
10.1016/s0040-8166(98)80069-4subject
Has Abstractpub_date
1998-04-01 00:00:00pages
205-15issue
2eissn
0040-8166issn
1532-3072pii
S0040-8166(98)80069-4journal_volume
30pub_type
杂志文章相关文献
TISSUE & CELL文献大全abstract：:Proteolytic processing of vitellin in Blattella germanica embryos is accomplished by activation of a yolk-borne cysteine protease (Mr 29 000) derived from a pro-protease precursor of Mr 40 000 (Liu et al., 1997). In the present study, fat body, ovaries and embryos of different developmental stages were examined immuno...
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1016/s0040-8166(97)80005-5
更新日期：1997-06-01 00:00:00
abstract：:The hydrogenosome, an organelle that produces molecular hydrogen and ATP from the oxidation of pyruvate or malate under anaerobic conditions, presents some characteristics common to mitochondria. The hydrogenosome of Tritrichomonas foetus, a cattle parasite, is a spherical organelle that presents a peripheral vesicle ...
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1016/j.tice.2007.09.006
更新日期：2008-02-01 00:00:00
abstract：:The spermatozoon of the Onychophoran Peripatopsis moseleyi is described. This cell is characterized by the general filiform shape, the absence of an acrosome, the presence of mitochondrial derivatives, of an annulus and of nine accessory tubules and a manchette of microtubules around the axoneme. All of these characte...
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1016/0040-8166(76)90038-0
更新日期：1976-01-01 00:00:00
abstract：:Enzymatically dissociated hamster tracheal epithelial (HTE) cells were cultured on collagen coated Millicell filters. Within 3-5 days after being placed in culture large numbers of ciliated and mucus cells began to appear. By 1 week the HTE cells closely resembled those seen in vivo, i.e. columnar morphology and organ...
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1016/0040-8166(87)90019-x
更新日期：1987-01-01 00:00:00
abstract：:Female adults of the bagworm moth, Eumeta variegata, lack wings completely, whereas male adults of this species have functional wings. We previously found that ecdysteroid induces apoptotic events in the female wing rudiment of E. variegata in vitro, whereas the male wing discs cultured with 20-hydroxyecdysone (20E) u...
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1016/j.tice.2011.01.003
更新日期：2011-06-01 00:00:00
abstract：:The role of the submandibular glands in the excretion of parenterally administered bis (tributyltin) oxide (TBTO) was studied. Fine structural alterations of the submandibular glands were observed with an electron microscope. Accumulation sites of TBTO were determined with an X-ray microanalyzer and tin concentrations...
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1016/0040-8166(92)90044-8
更新日期：1992-01-01 00:00:00
abstract：:Aromatization of androgens into estrogens in rat testis is catalyzed by the microsomal enzyme cytochrome P450 aromatase. In this work, aromatase cellular site was investigated in prepuberal, peripuberal and postpuberal testis, from 10-, 21- and 60-day-old rats respectively. Paraffin-embedded testis sections were proce...
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1054/tice.2001.0186
更新日期：2001-08-01 00:00:00
abstract：:We examined the development of serous (poison) cutaneous glands in larval and juvenile Scinax nasica (Hylidae) at the ultrastructural level. We describe the biosynthesis and maturation of the cutaneous poison in comparison with the corresponding processes in representatives of Discoglossidae, Leptodactylidae, Pelobati...
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1016/s0040-8166(03)00049-1
更新日期：2003-08-01 00:00:00
abstract：:Locust-style and pseudo-orthogonal structures were found in the cuticle of Heteropteryx dilatata, with the tibiae and femora containing both, and the ocular facets showing only helicoidal construction. The same epidermal cells appear to be competent to secrete both locust-style and pseudo-orthogonal cuticle structure....
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1016/0040-8166(92)90103-e
更新日期：1992-01-01 00:00:00
abstract：:As a defensive reaction against predators the larvae of Zygaena trifolii Esper. 1783, release highly viscous fluid droplets out of cuticular cavities. The fluid appears on the cuticular surface upon contraction of the irritated segments, with no specialized muscles being involved. Two morphologically different types o...
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1016/0040-8166(85)90093-x
更新日期：1985-01-01 00:00:00
abstract：:The failure of regeneration of damaged liver in various end-stage liver diseases results in high morbidity and mortality. In this context, we have demonstrated the differentiation ability of human hematopoietic stem cells (HSCs) into hepatocytes. In this study, HSCs were isolated from a donor and cultured which exhibi...
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1016/j.tice.2019.101312
更新日期：2020-02-01 00:00:00
abstract：:In Drosophila subobscura the male produces two classes of motile spermatozoa that differ in total length and nucleus length. The significance of this within-ejaculate polymegaly is obscure. We have carried out an ultrastructural and cytochemical analysis of both sperm morphs to understand their possible role at fertil...
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1016/s0040-8166(96)80005-x
更新日期：1996-04-01 00:00:00
abstract：:The elasmoid scales of the cichlid fish, Hemichromis bimaculatus, are localized within dermal pockets, the floors of which are separated from the stratum compactum by uninterrupted cellular sheets, the scale-pocket linings (SPL). TEM study of the fry skin shows that the SPL cells originate from the cell population con...
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1016/0040-8166(89)90058-x
更新日期：1989-01-01 00:00:00
abstract：:The transformation of the slow contracting larval m. obliquus lateralis caudalis II during metamorphosis into the asynchronous indirect flight muscle, m. obliquus lateralis dorsalis, in the Colorado beetle, Leptinotarsa decemlineata, was examined by electron microscopy. Particular attention was paid to the fate of the...
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1016/0040-8166(86)90066-2
更新日期：1986-01-01 00:00:00
abstract：:Despite profound knowledge of the incidence of oral cancers and a large body of research beyond it, it continues to beat diagnosis and treatment management. Post physical observation by clinicians, a biopsy is a gold standard for accurate detection of any abnormalities. Towards the application of artificial intelligen...
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1016/j.tice.2019.101322
更新日期：2020-04-01 00:00:00
abstract：:Bovine pulmonary artery endothelial cells in culture were evaluated by phase-contrast and electron microscopy at various times after being irradiated with 137Cs in vitro. Cells irradiated prior to reaching confluency showed vacuolization aand increased numbers of lysosomes beginning at 48 hr after irradiation with 300...
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1016/0040-8166(83)90016-2
更新日期：1983-01-01 00:00:00
abstract：:The vascular endothelium as well as subendothelium are objects of many researches as it is directly involved in a multiplicity of physiological and pathological settings. Detailed study of endothelial function became feasible with the development of techniques to culture endothelial cells (EC) in vitro. Limitations of...
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1016/j.tice.2015.03.004
更新日期：2015-06-01 00:00:00
abstract：OBJECTIVE:The current study proposes an automated machine learning approach for the quantification of cells in cell death pathways according to DNA fragmentation. METHODS:A total of 17 images of kidney histological slide samples from male Wistar rats were used. The slides were photographed using an Axio Zeiss Vert.A1 ...
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1016/j.tice.2016.12.006
更新日期：2017-02-01 00:00:00
abstract：:Male germ cells in the testis of Holothuria leucospilota can be divided into 12 stages based on ultrastructure and patterns of chromatin condensation. The spermatogonium (Sg) is a spherical-shaped cell with a diameter of about 6.5-7microm. Its nucleus mostly contains euchromatin and small blocks of heterochromatin sca...
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1016/j.tice.2007.10.001
更新日期：2008-06-01 00:00:00
abstract：:Oligoryzomys flavescens, a common rodent of the temperate regions of South America, has been identified as a Hantavirus reservoir. There is still little information concerning its reproductive biology, which is essential to devise effective control measurement of natural populations. This rodent is a seasonal breeder ...
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1016/j.tice.2007.05.001
更新日期：2007-08-01 00:00:00
abstract：:The electron microscopical changes in the glial lacunar network that surrounds the large neurons of meso- and metacerebrum of land snail cerebral ganglia were considered, in order to get an insight into the functional role of this peculiar structure in invertebrates. Compared with snails during the active period, in t...
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1016/s0040-8166(97)80056-0
更新日期：1997-10-01 00:00:00
abstract：:The present study reports on the ultrastructure features of spermatozoa and spermatogenesis of several species of Dysderidae (Dysdera crocata, Dysdera erythrina, Dysdera ninnii, Harpactea arguta, Harpactea piligera, Dasumia taeniifera). Dysderid spiders are known to possess a peculiar sperm transfer form known as syns...
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1016/j.tice.2004.08.001
更新日期：2004-12-01 00:00:00
abstract：:The hydrozoa life cycle is characterized, in normal conditions, by the alternation of a post-larval benthic polyp and an adult pelagic medusa; however, some species of Hydrozoa react to environmental stress by reverting their life cycle: i.e. an adult medusa goes back to the juvenile stage of polyp. This very uncommon...
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1016/s0040-8166(03)00028-4
更新日期：2003-06-01 00:00:00
abstract：:We have reported that telomere fluorescence units (TFUs) of established induced pluripotent stem cells (iPSCs) derived from human amnion (hAM933) and fetal lung fibroblasts (MRC-5) were significantly longer than those of the parental cells, and that the telomere extension rates varied quite significantly among clones ...
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1016/j.tice.2014.08.005
更新日期：2014-12-01 00:00:00
abstract：:Blood cells from three different sea turtle species were cultured for approximately 3 weeks in nutrient medium supplemented with recombinant human cytokines known to induce terminal maturation of human hematological stem cells. Cultured turtle erythrocytes were translucent, approximately 10x larger than human erythroc...
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1016/j.tice.2008.12.004
更新日期：2009-08-01 00:00:00
abstract：:When adult rat hepatocytes were cultured in plastic Petri dishes in a medium containing insulin and glucagon, supplementation with epidermal growth factor (EGF) had a pronounced effect on their viability, morphology, and biochemical integrity. Transmission and scanning electron microscopic studies showed that after 1 ...
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1016/0040-8166(84)90040-5
更新日期：1984-01-01 00:00:00
abstract：:Lithium Chloride (LiCl) has been used as a canonical Wnt pathway activator due to its ability to inhibit a glycogen synthase kinase-3. The aim of the present study was to investigate the effect of LiCl on cell proliferation and osteogenic differentiation in stem cells isolated from human exfoliated deciduous teeth (SH...
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1016/j.tice.2016.08.005
更新日期：2016-10-01 00:00:00
abstract：:The Piau boar is a rustic breed of economical importance in Brazil. The duration of spermatogenesis and sperm transit through the epididymis in Piau boars was estimated using intratesticular injections of tritiated thymidine. Animals were sacrificed 1 h, 7 days, 14 days, 21 days, 34 days, and 36 days after injections....
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1016/s0040-8166(98)80038-4
更新日期：1998-10-01 00:00:00
abstract：:In the present investigation, we have studied peroxisomes and sterol carrier protein-2 (SCP2) in control and luteinizing hormone stimulated rat luteal cells. Superovulated immature rats in mid-luteal phase (8 days after ovulation) were divided into two groups (n = 4/group) and treated with vehicle (0.2 ml saline), or ...
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1016/s0040-8166(05)80056-4
更新日期：1995-10-01 00:00:00
abstract：:The watery vacuoles first described from larval insect fat body (Chironomus, Voinov, 1927; Aedes, Wigglesworth, 1942; Rhodnius, Wigglesworth, 1967) have been studied in 4th and 5th stage Calpodes larvae. The vacuoles arise at the beginning (E + 6-24 hr) of the 4th stadium from plasma membrane infolds that separate fro...
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1016/0040-8166(83)90039-3
更新日期：1983-01-01 00:00:00
