Abstract：
:The vascular endothelium as well as subendothelium are objects of many researches as it is directly involved in a multiplicity of physiological and pathological settings. Detailed study of endothelial function became feasible with the development of techniques to culture endothelial cells (EC) in vitro. Limitations of this approach have become apparent with the realization that cell culture dedifferentiate with time and do not exhibit properties of intact tissue. Here we describe the development of a novel ex vivo tissue model to study cell-vascular wall interactions by using isolated mouse aorta patches. Validation of this model was performed by demonstrating cell attachment and changes in cell shape typical for cell spreading during adhesion. A major advantage of this model is that cell-endothelium interaction and its molecular backgrounds can now be studied more feasibly on an intact and native tissue.
journal_name
Tissue Celljournal_title
Tissue & cellauthors
Lin YN,Thata RN,Failla AV,Geissen M,Daum G,Windhorst Sdoi
10.1016/j.tice.2015.03.004subject
Has Abstractpub_date
2015-06-01 00:00:00pages
266-72issue
3eissn
0040-8166issn
1532-3072pii
S0040-8166(15)00022-1journal_volume
47pub_type
杂志文章相关文献
TISSUE & CELL文献大全abstract：:The tropharium of the common shorebug Saldula saltatoria consists of 2 zones: the apical mitotic region and the distal one comprising numerous mononucleate nurse cells. Each individual nurse cell is connected to the centrally located trophic core by a thin cytoplasmic projection referred to as a trophic process. The a...
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1016/0040-8166(90)90023-3
更新日期：1990-01-01 00:00:00
abstract：:Cell surface expression of the epidermal growth factor (EGF) receptor in several cell lines declines as a function of increased cell density and is associated with diminished responsiveness to EGF. However, the mechanism whereby this density-induced down regulation of receptors occurs has not been discerned. In the pr...
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1016/0040-8166(93)90061-o
更新日期：1993-01-01 00:00:00
abstract：:We have performed studies to determine if different populations of satellite cells provide nuclei to growing and regenerating skeletal muscle fibers. Satellite cells were isolated from regenerating or growing anterior tibialis muscles, and their phenotypic properties were compared in vitro. Isolates from regenerating ...
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1016/s0040-8166(96)80057-7
更新日期：1996-10-01 00:00:00
abstract：:An improved histochemical method for uric acid consists in precipitation as silver magnesium urate combined with fixation of the tissues in formol/glutaraldehyde followed by argentaffin reaction with silver nitrate buffered with "tris" to pH 9.5. This reveals urates both in solid deposits and in solution in the tissue...
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1016/0040-8166(87)90059-0
更新日期：1987-01-01 00:00:00
abstract：:Female adults of the bagworm moth, Eumeta variegata, lack wings completely, whereas male adults of this species have functional wings. We previously found that ecdysteroid induces apoptotic events in the female wing rudiment of E. variegata in vitro, whereas the male wing discs cultured with 20-hydroxyecdysone (20E) u...
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1016/j.tice.2011.01.003
更新日期：2011-06-01 00:00:00
abstract：:The second half of the 20th century has witnessed the birth and growth of biological ultrastructure research--a branch of cell biology in which electron microscopy plays an important role. After a humble start in around 1950, when only a limited arsenal of instrumentation was available, a wealth of auxiliary methodolo...
journal_title：Tissue & cell
pub_type： 历史文章,杂志文章,评审
doi：10.1016/j.tice.2003.11.001
更新日期：2004-04-01 00:00:00
abstract：:We used specific markers and fluorescence microscopy to identify and characterize cerebrovascular cells. Cultures were derived from brain microvessels isolated from normotensive (Wistar Kyoto, WKY) and spontaneously hypertensive (SHR) rat brains prior to, coincident with and following the onset of chronic hypertension...
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1016/0040-8166(87)90005-x
更新日期：1987-01-01 00:00:00
abstract：:The sensory organs on the tarsi of the antenniform first legs of the whip spider Admetus pumilio C. L. Koch (Amblypygi, Arachnida) were examined with the scanning and transmission electron microscope. At least four different types of hair sensilla were found: (1) thick-walled bristles, which have the characteristics o...
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1016/0040-8166(75)90009-9
更新日期：1975-01-01 00:00:00
abstract：:Tubules of the octopod posterior salivary gland are lined by two distinct epithelia, type A and B of which type A is predominant. A positive chromaffin reaction is given only by a small proportion of columnar cells of the type A epithelium, and is apparently associated with the large (3mu) cytoplasmic granules of thes...
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1016/s0040-8166(71)80040-x
更新日期：1971-01-01 00:00:00
abstract：:The reproductive system of the female Urodacus manicatus (Scorpionidae) is described. Situated on the ventral side of the ovariuterus tubes are buds and diverticulae belonging to three developmental phases: (1) Bud-like, rudimentary diverticulae that will form future generations. (2) Embryonic diverticulae that will h...
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1016/0040-8166(94)90060-4
更新日期：1994-10-01 00:00:00
abstract：:Bone substitutes are used in nearly half of all musculoskeletal surgeries. The "gold standard" graft is an autograft that is limited by supply and site morbidity. Therefore, allograft sources are the current alternative for clinical practice with some side effects, such as immune responses and risk of disease transmis...
journal_title：Tissue & cell
pub_type： 杂志文章,评审
doi：10.1016/j.tice.2021.101494
更新日期：2021-01-16 00:00:00
abstract：:We have reported that telomere fluorescence units (TFUs) of established induced pluripotent stem cells (iPSCs) derived from human amnion (hAM933) and fetal lung fibroblasts (MRC-5) were significantly longer than those of the parental cells, and that the telomere extension rates varied quite significantly among clones ...
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1016/j.tice.2014.08.005
更新日期：2014-12-01 00:00:00
abstract：:The detailed structure of the cockroach spermatheca is described and discussed firstly as an example of an insect integumentary gland, and secondly, from the standpoint of its role in reproduction. The gland comprises a cortical rank of separate secretory units, each associated with an epithelial duct cell responsible...
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1016/s0040-8166(69)80027-3
更新日期：1969-01-01 00:00:00
abstract：:We investigated apoptotic effects and changes in glucose-6-phosphate dehydrogenase (G6PD) enzyme activity in liver and gill tissues of fish exposed to chlorpyrifos. Three different chlorpyrifos doses (2.25, 4.5 and 6.75 μg/L) were administrated to rainbow trout at different time intervals (24, 48, 72 and 96 h). Acute ...
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1016/j.tice.2014.09.001
更新日期：2014-12-01 00:00:00
abstract：:Bilateral pairs of specific neurones in the metathoracic ganglion of the cockroach were used to study the ultrastructural changes which follow axon injury. The appearance of these matched pairs of cells was compared three days after the axons on one side of the ganglion only had been severed. The control or uninjured ...
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1016/s0040-8166(70)80039-8
更新日期：1970-01-01 00:00:00
abstract：:The primary musculature of Sagitta is mainly made up of two kinds of alternating fibers, A and B. These fibers differ markedly in their localization in the muscular tissue, by the development of their SR and their mitochondria, and the shape of their myofibrils. Their contractile apparatus is similar and possesses myo...
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1016/0040-8166(80)90025-7
更新日期：1980-01-01 00:00:00
abstract：:There is no effective therapy exists for Idiopathic pulmonary fibrosis (IPF) till now. Few studies have been done on protective effects of green tea in pulmonary fibrosis but there is no single report on black tea extract (BTE) in pulmonary fibrosis so far. This study aims to investigate the anti-fibrotic effect of BT...
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1016/j.tice.2018.11.006
更新日期：2019-02-01 00:00:00
abstract：:The loss of oligodendrocyte progenitor cells (OPC) is a hallmark of perinatal brain injury. Our aim was to develop an in vitro culture condition for human chorion-derived mesenchymal stem cells (MSC) that enhances their stem cell properties and their capability to differentiate towards OPC-like cells. MSC were grown e...
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1016/j.tice.2018.04.002
更新日期：2018-06-01 00:00:00
abstract：:As part of an ongoing comparative fine structural study, the retinal pigment epithelium (RPE), choriocapillaris and complexus basalis (Bruch's membrane) in the emu (Dromaius novaehollandiae) have been investigated by light and electron microscopy. The RPE consists of a single layer of cuboidal cells joined basally by ...
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1016/s0040-8166(98)80063-3
更新日期：1998-04-01 00:00:00
abstract：:In all insects investigated hitherto the mechanoreceptive hair sensilla possess the same organization plan. There are differences in the moulting process of hemimetabolous insects. While the functions of the three enveloping cells are corresponding, the position of the moulting porus is different. In Gryllus and Perip...
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1016/s0040-8166(96)80039-5
更新日期：1996-02-01 00:00:00
abstract：:Harderian glands exist in the orbits of most terrestrial vertebrates. The basic function of the gland is the lubrication of the eye. The present study was carried out to shed some light on the ultrastructure of the still enigmatic Harderian gland of the lizard Uromastyx microlepis, a common species in Kuwait and other...
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1054/tice.1999.0088
更新日期：2000-02-01 00:00:00
abstract：:Until primate sperm are exposed to the unique microenvironment of the epididymis, they are not capable of fertilization or vigorous motility. Many of the proteins that contribute to the unique microenvironment of the primate epididymis, and thus to sperm maturation, are dependent on androgens to induce their synthesis...
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1054/tice.2000.0164
更新日期：2001-06-01 00:00:00
abstract：:Intratubular injection of electron-opaque tracers (lanthanum hydroxide, peroxidase) by micromanipulation showed that the intercellular spaces of the adluminal compartment are in continuity with the lumen of the rat seminiferous tubule at all stages of the spermatogenic cycle. This continuity involves the intercellular...
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1016/0040-8166(88)90059-6
更新日期：1988-01-01 00:00:00
abstract：:A detailed model for hyposmotic fluid formation in Hydra is presented. We propose that enteron fluid formation occurs in two steps: (1) segregation of an isosmotic fluid in large intercellular vacuoles with (2) subsequent reabsorption of solute in the intercellular channels to form the hyposmotic fluid of the enteron....
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1016/0040-8166(77)90045-3
更新日期：1977-01-01 00:00:00
abstract：:The periostracum in Unionidae consists of two layers. The outer one is secreted within the periostracal groove, while the inner layer is secreted by the epithelium of the outer mantle fold. The periostracum reaches its maximum thickness at the shell edge, where it reflects onto the shell surface. Biomineralization beg...
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1054/tice.2000.0129
更新日期：2000-10-01 00:00:00
abstract：:The teleost species Notopterus notopterus (Pallas) possess bundled photoreceptors in their retina. It was found that the margin of the inner portion (the vitreal half) of photoreceptor bundles emits thin processes. Each process terminates on the contralateral photoreceptor bundle, or the processes from adjacent photor...
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1016/j.tice.2019.03.003
更新日期：2019-06-01 00:00:00
abstract：:The presence of corticotropin-releasing factor-like material in the intermaxillary glands was studied by immunocytochemical techniques during the metamorphosis of Bufo arenarum. The intermaxillary glands appeared at stage XV (midprometamorphosis) with CRF-like material slightly immunoreactive. These glands are located...
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1054/tice.2000.0098
更新日期：2000-04-01 00:00:00
abstract：:With scanning electron microscopy the spermathecal organ of Sitophilus granarius (L.) has been examined. The only morphological attachment between this organ and other tissues is to the bursa copulatrix by the sperm duct and to the central nervous system by two nerve fibers. The nerves innervate the muscles connecting...
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1016/s0040-8166(71)80002-2
更新日期：1971-01-01 00:00:00
abstract：:Sporogony of the sporozoan Theileria parva in the salivary gland of the tick vector of East Coast fever was studied in electron micrographs. The findings differ in several respects from previous interpretations based upon light microscopy. Cytokinesis of the primary sporoblast to form secondary and tertiary sporoblast...
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1016/0040-8166(82)90017-9
更新日期：1982-01-01 00:00:00
abstract：:Three basic sublayers are identified in the epicuticle of the mineralised sclerites of the Atlantic shore crab Carcinus maenas (Crustacea, Decapoda): the surface coat, the cuticulin layer, and the inner epicuticle. Their morphogenesis and subsequent changes are described throughout the moulting cycle in the normal cut...
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1016/s0040-8166(05)80061-8
更新日期：1995-10-01 00:00:00
