Histochemical studies of uric acid in some insects. I. Storage in the fat body of Periplaneta americana and the action of the symbiotic bacteria.

Abstract:

:An improved histochemical method for uric acid consists in precipitation as silver magnesium urate combined with fixation of the tissues in formol/glutaraldehyde followed by argentaffin reaction with silver nitrate buffered with "tris" to pH 9.5. This reveals urates both in solid deposits and in solution in the tissues. Polyphenols concerned in sclerotin formation also react. In Periplaneta, uric acid synthesized in the trophocytes is carried by intracellular and intercellular channels to form the intercellular deposits of solid spheres. The symbiotic bacteria in the mycetocytes in contact with the deposits appear to metabolize the uric acid and they disperse and eliminate the deposits.

journal_name

Tissue Cell

journal_title

Tissue & cell

authors

Wigglesworth VB

doi

10.1016/0040-8166(87)90059-0

subject

Has Abstract

pub_date

1987-01-01 00:00:00

pages

83-91

issue

1

eissn

0040-8166

issn

1532-3072

pii

0040-8166(87)90059-0

journal_volume

19

pub_type

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