Abstract：
:During fixation of single muscles fibers with glutaraldehyde, the volume of the fiber shrinks 20%, recovers in rinse and osmium tetroxide to near normal volume and shrinks 20% again when staining with uranyl acetate. This suggest that osmotic properties of membranes may not have been completely lost during fixation, post-fixation and en bloc staining. Dehydration in ethanol and propylene oxide produces a further 10% shrinkage in volume. Infiltration and embedding with Epon causes an additional 15% change in volume. This gives a total shrinkage in volume of 45% which is nearly twice that of the apparent shrinkage in the volume of the myosin lattice as determined by electron microscopy.
journal_name
Tissue Celljournal_title
Tissue & cellauthors
Eisenberg BR,Mobley BAdoi
10.1016/0040-8166(75)90013-0subject
Has Abstractpub_date
1975-01-01 00:00:00pages
383-7issue
2eissn
0040-8166issn
1532-3072pii
0040-8166(75)90013-0journal_volume
7pub_type
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