Ultrastructural localisation of alkaline phosphatase in the intertubular tissue of the testis in the domestic fowl.

Abstract:

:Alkaline phosphatase activity in the intertubular tissue of the testes of the domestic fowl was examined using an ultracytochemical technique based on the lead capture method. In the interstitial tissue, the Leydig cells, transitional cells and the fibroblasts displayed enzyme activity on their cell membranes. Vacuoles located in the transitional cells were lined by reaction products of enzyme activity, whereas the vacuoles representing extracted lipid droplets and present mainly in the Leydig cells were free of enzyme activity. In the peritubular tissue the cell processes of fibroblasts showed enzyme activity on the cell membranes and in pinocytotic vesicles. Cell processes lying adjacent to blood vessels showed pronounced activity. In the blood vessel itself some activity was present in the basement membrane and the endothelium. The surface of the red blood cell showed moderate activity. The possible role of alkaline phosphatase in the transfer of hormone from the Leydig cells to the seminiferous tubules and from the seminiferous tubules to the interstitium is discussed. The myoid cells and their processes were devoid of enzyme activity.

journal_name

Tissue Cell

journal_title

Tissue & cell

authors

Gunawardana VK

doi

10.1016/0040-8166(90)90015-2

subject

Has Abstract

pub_date

1990-01-01 00:00:00

pages

113-21

issue

2

eissn

0040-8166

issn

1532-3072

pii

0040-8166(90)90015-2

journal_volume

22

pub_type

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