Abstract:
:An intestinal epithelial cell line (IEC-17), undergoing a process of progressive morphological differentiation, was analysed for expression and synthesis of the extracellular matrix glycoproteins, fibronectin (FN) and laminin (LM). FN and LM cell surface expression was detected by immunoelectron microscopy, while intracytoplasmic accumulation was shown by immunofluorescence. 35S-methionine metabolic labelling was also performed to demonstrate FN and LM synthesis by IEC-17. We have compared two different maturation stages of the cell culture and have found that either early epithelial monolayer cells or later multistratified organoid structure cells expressed and produced large amounts of both proteins. These results indicate that FN and LM are constantly present during the process of IEC-17 organoid maturation: we can hypothesize that the two proteins act as mediators of cell to cell and cell to substrate adhesion interactions and, probably, have an active regulatory role in the process of intestinal epithelial cell differentiation.
journal_name
Tissue Celljournal_title
Tissue & cellauthors
Scarpa S,D'Orazi G,Modesti A,Valia S,Zucco F,Stammati Adoi
10.1016/0040-8166(88)90066-3subject
Has Abstractpub_date
1988-01-01 00:00:00pages
305-12issue
3eissn
0040-8166issn
1532-3072pii
0040-8166(88)90066-3journal_volume
20pub_type
杂志文章相关文献
TISSUE & CELL文献大全abstract::Using a monoclonal antibody (GT 335) we previously demonstrated that glutamylation is a predominant posttranslational modification of alpha and beta tubulin isoforms in the axoneme of mouse spermatozoa (Fouquet et al., Cell Motil. Cytoskel. 27, 49, 1994). However, we noted that the staining intensity and/or distributi...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/s0040-8166(95)80053-0
更新日期:1995-06-01 00:00:00
abstract::The ventral nerve cord of the spiny lobster, Panulirus argus was examined by transmission and scanning electron microscopy. Tannic acid mordant stain was used to enhance extracellular filaments. The ventral nerve cord is surrounded by an unusual perineurial sheath composed primarily of interwoven extracellular filamen...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/0040-8166(90)90013-y
更新日期:1990-01-01 00:00:00
abstract::Vitellogenic oocytes from the cockroach Blattella germanica were dissected free of the surrounding follicle cell layer. The continued presence of a resting potential indicated that the defolliculated oocytes remained viable. In other experiments, lysozyme was injected into last nymphal instars; scanning electron micro...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/0040-8166(90)90009-x
更新日期:1990-01-01 00:00:00
abstract::1. To be mechanically effective, supporting structures which are helicoidal need to be monodomain, with planar or concentric layers. 2. To achieve this in cholesteric liquid crystalline chemical models, a constraining surface is required. 3. The prediction which logically follows from this is that natural helicoidal s...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/0040-8166(88)90013-4
更新日期:1988-01-01 00:00:00
abstract::During early-to-middle spermiogenesis in multicellular, internally fertilizing charalean green algae (Chara fibrosa, Chara vulgaris, Chara tomentosa, Nitella missouriensis), patterning of chromatin/nucleoplasm in developing spermatid nuclei changes from granules → fibers → contorted lamellae → condensed chromatin. Cyt...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/j.tice.2014.07.003
更新日期:2014-12-01 00:00:00
abstract::In the central canal, F-actin is predominantly localized in the apical region, forming a ring-like structure around the circumference of the lumen. However, an exception is found in the medulla oblongata, where the apical F-actin becomes interrupted in the ventral aspect of the canal. To clarify the precise localizati...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/j.tice.2017.01.005
更新日期:2017-04-01 00:00:00
abstract::The immunohistochemical distribution of galanin (Gal) in the brain and pituitary of Rhinella arenarum was studied during development. Gal-immunoreactivity was first observed in the brain just after hatching in anterior preoptic area, infundibular area, median eminence and pars distalis of the pituitary as well as in t...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/j.tice.2008.03.002
更新日期:2008-10-01 00:00:00
abstract::The presence of stage-dependent occlusive junctions between adjacent Sertoli cells in the seminiferous epithelium of the crayfish testis was demonstrated by a lanthanum tracer study. The germinal epithelium did not appear to be compartmentalized, as evidenced by access of lanthanum to spermatogonia, spermatocytes, and...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1054/tice.2001.0211
更新日期:2001-12-01 00:00:00
abstract::Germ cells' proliferation during testicular organogenesis in Wistar rat embryos and neonates [14.5, 18.5, 20.5 days post conception (dpc), birth (day 0), 1, 3, 5, 7 days post partum (dpp)] was evaluated via immunohistochemistry, using the PCNA and Ki-67 nuclear antibodies. Estimation of the reactive/total cell ratio, ...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/j.tice.2007.09.004
更新日期:2008-02-01 00:00:00
abstract::The morphology of the extensible intersegmental membrane (i.s.m.) of the female Locusta migratoria migratorioides is dictated by three main requirements: (a) high extensibility (1500%), (b) low stiffness (5 X 10(3) Pa), (c) low Poisson ratio in the plane of the cuticle (0.01 or less). These requirements can be met onl...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/s0040-8166(81)80017-1
更新日期:1981-01-01 00:00:00
abstract::The basal plate of the scales of Amia calva is composed of regular double twisted plywood, as in Latimeria and Dipnoan scales. However, the progressive rotation of the fibrils direction is left-handed in Amia and right-handed in the 'Sarcopterygians'. So, the similarity between these peculiar plywoods is probably the ...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/0040-8166(81)90046-x
更新日期:1981-01-01 00:00:00
abstract::The number of epithelial cells comprising larval midgut of the tobacco hornworm moth, Manduca sexta increases 200-fold in development from the first to the fifth instar. We have examined larvae periodically before and during molting to follow epithelial cell proliferation and differentiation. The midgut epithelium in ...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/0040-8166(91)90058-2
更新日期:1991-01-01 00:00:00
abstract::Moist Exposed Burn Ointment (MEBO(®)) is widely used topical agent applied on skin burn. This study investigated the effect of MEBO topical application on activation and proliferation of epidermal stem cells through the immunohistochemical localization of cytokeratin 19 (CK19) as a known marker expressed in epidermal ...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/j.tice.2014.01.002
更新日期:2014-04-01 00:00:00
abstract::In order to determine the extent and variations in surface concavanalin A (CON A) and wheat germ agglutinin (WGA) labeling of different varieties of normal blood cells, gluraraldehyde-fixed human blood cells were exposed to CON A-gold labeled horseradish peroxidase (CON A-HRP-G) and WGA-gold labeled ovomucoid (WGA-OVO...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/0040-8166(83)90042-3
更新日期:1983-01-01 00:00:00
abstract::The watery vacuoles first described from larval insect fat body (Chironomus, Voinov, 1927; Aedes, Wigglesworth, 1942; Rhodnius, Wigglesworth, 1967) have been studied in 4th and 5th stage Calpodes larvae. The vacuoles arise at the beginning (E + 6-24 hr) of the 4th stadium from plasma membrane infolds that separate fro...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/0040-8166(83)90039-3
更新日期:1983-01-01 00:00:00
abstract::Annulate lamellae have been found in a primitive neuroectodermal tumor, a metastatic cerebellar tumor, a testicular seminoma, a retinoblastoma and three melanomas. These annulate lamellae are arranged in stacked parallel arrays in the cytoplasm of tumor cells. The number of annulate lamellae observed to comprise a sin...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/0040-8166(79)90014-4
更新日期:1979-01-01 00:00:00
abstract::Treatment of massive bone defects is one of the most difficult problems to solve in orthopedics. At present, there is no consensus on the best way to resolve these problems. The aim of our study was to evaluate the effect of a three-dimensional bioimplant over massive bone defects, and to analyse if it improves the sp...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/j.tice.2017.12.005
更新日期:2018-02-01 00:00:00
abstract::This paper describes the fine structure of the cockroach subgenual organ, a complex ciliated mechanoreceptor that detects vibrations in the substrate upon which the animal stands. Located beneath the knee in each walking leg, the cockroach subgenual organ is a thin, fan-shaped flap of tissue slung across the dorsal bl...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/s0040-8166(75)80009-7
更新日期:1975-01-01 00:00:00
abstract::Spermatozoa and spermiogenesis of the prithine filistatid spider Wandella orana are described. The spider produces coenospermia, i.e. sperm aggregations that include several single sperm cells commonly surrounded by a secretion sheath. One sectioned coenospermium in W. orana contains at least five spermatozoa. During ...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/s0040-8166(03)00054-5
更新日期:2003-10-01 00:00:00
abstract::REM and TEM studies of the subgenual organ in Chrysoperla carnea (Neuroptera: Chrysopidae) show that it is composed of three scolopidia, each with one sensory, one scolopale and one cap cell. The distal part of the dendrite shows a cilium with a '9 + 0' structure. The cross-handing pattern of the ciliary root has a pe...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/0040-8166(94)90100-7
更新日期:1994-04-01 00:00:00
abstract::The electron microscopical changes in the glial lacunar network that surrounds the large neurons of meso- and metacerebrum of land snail cerebral ganglia were considered, in order to get an insight into the functional role of this peculiar structure in invertebrates. Compared with snails during the active period, in t...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/s0040-8166(97)80056-0
更新日期:1997-10-01 00:00:00
abstract::Glossina austeni oogenesis throughout its nine-day pregnancy cycle is described with the focus on previtellogenic stages. The ultrastructural details of the oocyte-nurse cell relationship and cyst formation is presented. The oocyte develops in a syncytial association with 15 nurse cells with the entire unit surrounded...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/0040-8166(75)90007-5
更新日期:1975-01-01 00:00:00
abstract::The structure of the sclerite and intersegmental cuticle of the opithosoma of the desert scorpion, Hadrurus arizonensis, has been examined by transmission electron microscopy. The sclerite cuticle contains a four-layered epicuticle, a hyaline exocuticle, an inner exocuticle and an endocuticle. The outer part of the hy...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/0040-8166(79)90040-5
更新日期:1979-01-01 00:00:00
abstract::The hydrogenosome, an organelle that produces molecular hydrogen and ATP from the oxidation of pyruvate or malate under anaerobic conditions, presents some characteristics common to mitochondria. The hydrogenosome of Tritrichomonas foetus, a cattle parasite, is a spherical organelle that presents a peripheral vesicle ...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/j.tice.2007.09.006
更新日期:2008-02-01 00:00:00
abstract::The periostracum in Unionidae consists of two layers. The outer one is secreted within the periostracal groove, while the inner layer is secreted by the epithelium of the outer mantle fold. The periostracum reaches its maximum thickness at the shell edge, where it reflects onto the shell surface. Biomineralization beg...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1054/tice.2000.0129
更新日期:2000-10-01 00:00:00
abstract::Two fracture faces in each half of the freeze-fractured tegumental membrane of adult Schistosoma mansoni indicate the presence of two trilaminate membranes. This result is compatible with the heptalaminate appearance of the tegumental membrane in ultrathin sections. Intramembranous particles are located mainly in the ...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/0040-8166(75)90020-8
更新日期:1975-01-01 00:00:00
abstract::Junctional complexes between the epithelial cells in the four distinct regions of the glow-worm Malpighian tubule were investigated by electron microscopy using thin sectioning, freeze-fracturing, osmotic disruption and tracer techniques. The lateral plasma membranes of all four cell types are joined by smooth septate...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/0040-8166(80)90011-7
更新日期:1980-01-01 00:00:00
abstract::Spermiogenesis of the domestic sparrow was investigated with the light and electron microscopes and a step by step classification is proposed. Three cell populations corresponding to early, mid and late spermatids were easily divided according to their positions in the seminiferous epithelium. In addition to this init...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/s0040-8166(02)00017-4
更新日期:2002-08-01 00:00:00
abstract::The distribution of F-actin and intermediate filaments in the W/WV mouse was investigated by light and transmission electron microscopy, and fluorescence methods. No spermatogenic cells were detected in the seminiferous epithelium of the W/WV mouse. Its seminiferous tubule was one-half the diameter of that in the norm...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/0040-8166(92)90024-2
更新日期:1992-01-01 00:00:00
abstract::Malaria, one of the leading causes of death in underdeveloped countries, is primarily diagnosed using microscopy. Computer-aided diagnosis of malaria is a challenging task owing to the fine-grained variability in the appearance of some uninfected and infected class. In this paper, we transform a malaria parasite objec...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/j.tice.2020.101473
更新日期:2020-12-31 00:00:00