Abstract:
:Treatment of massive bone defects is one of the most difficult problems to solve in orthopedics. At present, there is no consensus on the best way to resolve these problems. The aim of our study was to evaluate the effect of a three-dimensional bioimplant over massive bone defects, and to analyse if it improves the speed and quality of integration in recipient bone compared to allograft treatment. Fifteen female lambs with massive bone defects, surgically created in their tibias, were randomly divided into three groups of five lambs each: Group I -treated with the bioimplant; Group 2 -treated with the bioimplant plus nucleated cells of autologous bone marrow; Group 3 -treated with a frozen allograft. Radiographs were taken post-treatment at weeks 1, 6, and 12. Animals were euthanized to obtain the studied bone segment for morphological analyses. TREATMENT:with bioimplants vs. bioimplant plus bone marrow nucleated cells (BMNCs) showed a notorious osteogenic effect, but with greater osteoid synthesis and cellularity in the latter. These results suggest that combined treatment with bioimplants and BMNCs have an additive effect on massive bone defects in lambs. These experimental results could be applied to repair damaged human bone.
journal_name
Tissue Celljournal_title
Tissue & cellauthors
Cuervo-Lozano CE,Soto-Domínguez A,Saucedo-Cárdenas O,Montes-de-Oca-Luna R,Alonso-Romero S,Del Consuelo Mancías-Guerra M,Álvarez-Lozano Edoi
10.1016/j.tice.2017.12.005subject
Has Abstractpub_date
2018-02-01 00:00:00pages
69-78eissn
0040-8166issn
1532-3072pii
S0040-8166(17)30314-2journal_volume
50pub_type
杂志文章相关文献
TISSUE & CELL文献大全abstract::Lipid bodies (LB), lipid-rich inclusions abundantly present in cells engaged in inflammation, are specialized intracellular domains involved in generating inflammatory mediators, the eicosanoids. Since the acute Trypanosoma cruzi infection triggers a potent inflammatory reaction characterized by a great increase of pe...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/s0040-8166(02)00105-2
更新日期:2003-02-01 00:00:00
abstract::Antiserum to leucokinin I, a neuropeptide originally isolated from the cockroach Leucophaea maderae, was used for immunocytochemical labeling of neurons in the brain and ventral ganglia of the moth Spodoptera litura during postembryonic development. In the ventral ganglia, leucokinin-like immunoreactivity begins to oc...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/s0040-8166(98)80008-6
更新日期:1998-02-01 00:00:00
abstract::The periostracum in Unionidae consists of two layers. The outer one is secreted within the periostracal groove, while the inner layer is secreted by the epithelium of the outer mantle fold. The periostracum reaches its maximum thickness at the shell edge, where it reflects onto the shell surface. Biomineralization beg...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1054/tice.2000.0129
更新日期:2000-10-01 00:00:00
abstract::The electrophysiological properties and structure of heart muscle fibres of the lobster myocardium have been investigated. Discontinuities were observed in the expected decrement of electrotonic potentials in a study of the passive membrane properties of the muscle. Injection of Procion yellow revealed transverse barr...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/s0040-8166(71)80018-6
更新日期:1971-01-01 00:00:00
abstract::Oligoryzomys flavescens, a common rodent of the temperate regions of South America, has been identified as a Hantavirus reservoir. There is still little information concerning its reproductive biology, which is essential to devise effective control measurement of natural populations. This rodent is a seasonal breeder ...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/j.tice.2007.05.001
更新日期:2007-08-01 00:00:00
abstract::The three-dimensional aspect of rat and monkey lung multilamellar bodies was demonstrated in lipid retained thin sections. The glutaraldehyde and urea lipid retention embedment and an Epon 812 resin polar dehydrant procedure were utilized to retain lamellar lipids for precise morphological study. The unextracted multi...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/0040-8166(76)90040-9
更新日期:1976-01-01 00:00:00
abstract::The immunohistochemical distribution of galanin (Gal) in the brain and pituitary of Rhinella arenarum was studied during development. Gal-immunoreactivity was first observed in the brain just after hatching in anterior preoptic area, infundibular area, median eminence and pars distalis of the pituitary as well as in t...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/j.tice.2008.03.002
更新日期:2008-10-01 00:00:00
abstract::Isolation and culture of Xenopus laevis neural tubes resulted in differentiation of melanophores and iridophores from neural crest cells; the differentiated melanophores and iridophores were then maintained in culture for more than 6 months. Guanosine has been reported to promote reflecting platelet formation in melan...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/j.tice.2018.08.003
更新日期:2018-10-01 00:00:00
abstract::A detailed model for hyposmotic fluid formation in Hydra is presented. We propose that enteron fluid formation occurs in two steps: (1) segregation of an isosmotic fluid in large intercellular vacuoles with (2) subsequent reabsorption of solute in the intercellular channels to form the hyposmotic fluid of the enteron....
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/0040-8166(77)90045-3
更新日期:1977-01-01 00:00:00
abstract::Vitellogenic oocytes from the cockroach Blattella germanica were dissected free of the surrounding follicle cell layer. The continued presence of a resting potential indicated that the defolliculated oocytes remained viable. In other experiments, lysozyme was injected into last nymphal instars; scanning electron micro...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/0040-8166(90)90009-x
更新日期:1990-01-01 00:00:00
abstract::We used immunofluorescence microscopy and isoactin-specific antibodies to characterize the pattern and prevalence of pericytes within the brain microcirculation. Blood pressures of normotensive, Wistar-Kyoto (WKY) and spontaneously hypertensive (SHR) rats were measured prior to sacrifice and pressure-perfusion fixatio...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/0040-8166(88)90002-x
更新日期:1988-01-01 00:00:00
abstract::Using microsurgical manipulations, hormone applications, and transmission electron microscopy we have investigated the regulation of differentiation of the follicular epithelium and formation of the vitelline envelope (VE) in primary follicles in the ovary of the mosquito, Aedes aegypti. During the first 3 days after ...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/0040-8166(91)90015-l
更新日期:1991-01-01 00:00:00
abstract::Due to the recent importance assumed by the apoptotic process as a tool in the therapeutic protocols, we investigated the onset and rate of drug-induced apoptosis in a cell model of prostatic tumor: the DU-145 cells. The human prostatic cancer cells, DU-145, have been treated with pro-apoptotic substances like puromyc...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/j.tice.2005.06.006
更新日期:2005-10-01 00:00:00
abstract::We have recently shown that down-regulation of mouse Thy28 (mThy28) protein expression appears to be accompanied by apoptotic processes. Thymocytes from mice contain moderate amounts of mThy28 protein and undergo proliferation, differentiation, or apoptosis during murine thymic maturation. As a first step to examine t...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/s0040-8166(03)00073-9
更新日期:2003-12-01 00:00:00
abstract::Rhodnius prolixus is an important vector of Trypanosoma cruzi, the etiological agent of Chagas disease. Insect adults have a pair of Metasternal Glands (MGs) and the secretion emitted by these glands acts as sex pheromone. Recent studies have focused on the chemical composition of this pheromone, electrophysiological ...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/j.tice.2020.101416
更新日期:2020-12-01 00:00:00
abstract::Sepsis was a systemic response to a local infection. Apoptosis was observed in the experimental sepsis. In this study, cecal ligation and puncture (CLP)-induced sepsis was established in rats. We found that sepsis decreased thyroid hormone levels, including triiodothyronine (T3), thyroxine (T4), free T3 (fT3), and fre...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/j.tice.2016.07.001
更新日期:2016-10-01 00:00:00
abstract::There is increasing evidence that type II alveolar cells are capable of synthesizing surface active material like that obtained from the airways. However a number of problems remain to be solved before it can be stated conclusively that type II cells synthesize the surface active material of the terminal airspace. Amo...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/0040-8166(75)90028-2
更新日期:1975-01-01 00:00:00
abstract::In tendon lesions, inflammation indicates the beginning of tissue repair and influences cell proliferation and the remodeling of the extracellular matrix (ECM). Low level laser (LLL) therapy has been an important method to induce tissue repair, and several studies have sought to better understand the therapeutic possi...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/j.tice.2017.05.006
更新日期:2017-08-01 00:00:00
abstract::The spermatozoon of the monopisthocotylean monogenean Pseudodactylogyrus sp. (a gill parasite of eels) has a single axoneme showing a 9 + '1' pattern, a nucleus and a mitochondrion, but has no cortical microtubules. This species thus provides a very simple model for the study of tubulin in the 9 + '1' axonemes of the ...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/s0040-8166(97)80045-6
更新日期:1997-12-01 00:00:00
abstract::Testicular peritubular tissue, also known as the tunica propria, surrounds the seminiferous tubules and is responsible for contractile, paracrine and transport functions. The aim of the present report is to describe the pathomorphology of peritubular tissue in association with tubular hyalinization in human testis. Tw...
journal_title:Tissue & cell
pub_type: 临床试验,杂志文章
doi:10.1054/tice.1999.0005
更新日期:1999-02-01 00:00:00
abstract::The main purpose of the article is to review recent knowledge about growth factors and their effect on the chondrogenic differentiation of mesenchymal stem cells under in vitro conditions. Damaged or lost articular cartilage leads to progressive debilitation, which have major impact on the life quality of the affected...
journal_title:Tissue & cell
pub_type: 杂志文章,评审
doi:10.1016/j.tice.2011.11.005
更新日期:2012-04-01 00:00:00
abstract::Intratubular injection of electron-opaque tracers (lanthanum hydroxide, peroxidase) by micromanipulation showed that the intercellular spaces of the adluminal compartment are in continuity with the lumen of the rat seminiferous tubule at all stages of the spermatogenic cycle. This continuity involves the intercellular...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/0040-8166(88)90059-6
更新日期:1988-01-01 00:00:00
abstract::The dynamics of human and animal adipogenesis has been defined using several traditional cell systems including stromal vascular cells and adipocyte-related cell lines. But a relatively new cell system using progeny cells stemming from the dedifferentiation of purified cultures of mature adipocytes may be used for stu...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/j.tice.2012.08.001
更新日期:2012-12-01 00:00:00
abstract::Aggregation factors, purified from 14 sponges which belong to the classes Tetraxonida and Cornacuspongia, Were visualized electron microscopically. Two types of basic structural forms were detected; first, circular structures from the species Ancorina cerebrum, Mycale massa, Hemimycale columella, Crella rosea, Clathri...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/0040-8166(82)90019-2
更新日期:1982-01-01 00:00:00
abstract::The reproductive system of the female Urodacus manicatus (Scorpionidae) is described. Situated on the ventral side of the ovariuterus tubes are buds and diverticulae belonging to three developmental phases: (1) Bud-like, rudimentary diverticulae that will form future generations. (2) Embryonic diverticulae that will h...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/0040-8166(94)90060-4
更新日期:1994-10-01 00:00:00
abstract::We have performed studies to determine if different populations of satellite cells provide nuclei to growing and regenerating skeletal muscle fibers. Satellite cells were isolated from regenerating or growing anterior tibialis muscles, and their phenotypic properties were compared in vitro. Isolates from regenerating ...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/s0040-8166(96)80057-7
更新日期:1996-10-01 00:00:00
abstract::Spermatogenesis and the sperm structure of the terebrantian Aeolothrips intermedius Bagnall are described. Spermatogenesis consists of two mitotic divisions; the second is characterized by the loss of half of the spermatids, which have pyknotic nuclei. Early spermatids have two centrioles, but when spermiogenesis star...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/j.tice.2010.04.008
更新日期:2010-08-01 00:00:00
abstract::Bilateral pairs of specific neurones in the metathoracic ganglion of the cockroach were used to study the ultrastructural changes which follow axon injury. The appearance of these matched pairs of cells was compared three days after the axons on one side of the ganglion only had been severed. The control or uninjured ...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/s0040-8166(70)80039-8
更新日期:1970-01-01 00:00:00
abstract::Changes in the fine structural organization of the pars intermedia related to the onset of secretory activity within the gland have been studied. It is shown that during the first seven days, following the onset of secretory activity, there is an extensive membraneogenesis within the cytoplasm of the parenchymatous ce...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/s0040-8166(70)80007-6
更新日期:1970-01-01 00:00:00
abstract::An intestinal epithelial cell line (IEC-17), undergoing a process of progressive morphological differentiation, was analysed for expression and synthesis of the extracellular matrix glycoproteins, fibronectin (FN) and laminin (LM). FN and LM cell surface expression was detected by immunoelectron microscopy, while intr...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/0040-8166(88)90066-3
更新日期:1988-01-01 00:00:00