Abstract：
:A cell line BmN-SWU1 was established from the ovarian tissues of 3-day-old fourth instar Bombyx mori larvae of the 21-872nlw variety by performing primary cultures in Grace's medium supplemented with 20% fetal bovine serum (FBS). The cell line primarily consisted of short spindle cells and round cells. The frequency of cells with chromosome number 2n=56 was 80.5%; therefore, the cell line was considered to be a diploid cell line. The population-doubling time (PDT) at 45th passage line was 57.7h. This cell line was susceptible to the B. mori nuclear polyhedrovirus (BmNPV), and the median tissue culture infective dose (TCID(50)) at a cell density of 10(5) cells/ml was 16.3 OBs/ml. The transient expression efficiency of the green fluorescent protein (GFP) gene in this cell line was 54.8%. We used the BmN-SWU1 cell line to select and establish a GFP transgenic cell line.
journal_name
Tissue Celljournal_title
Tissue & cellauthors
Pan MH,Cai XJ,Liu M,Lv J,Tang H,Tan J,Lu Cdoi
10.1016/j.tice.2009.07.002subject
Has Abstractpub_date
2010-02-01 00:00:00pages
42-6issue
1eissn
0040-8166issn
1532-3072pii
S0040-8166(09)00066-4journal_volume
42pub_type
杂志文章相关文献
TISSUE & CELL文献大全abstract：:The dynamics of human and animal adipogenesis has been defined using several traditional cell systems including stromal vascular cells and adipocyte-related cell lines. But a relatively new cell system using progeny cells stemming from the dedifferentiation of purified cultures of mature adipocytes may be used for stu...
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1016/j.tice.2012.08.001
更新日期：2012-12-01 00:00:00
abstract：:Many insect cells, including epidermis, fat body, ocnocytcs and pericardial cells, can very easily be induced to form long fine processes or filopodia. Filopodia contain microfilaments hut differ from epidermal feet in lacking microtubules and in having a much smaller and uniform diameter. Although they may be 10-30 m...
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1016/0040-8166(87)90014-0
更新日期：1987-01-01 00:00:00
abstract：:A brief description is given of the behavioural changes in Drosophila melanogaster at the time of eclosion and wing expansion. The developmental changes in the wing during this period are classified into four stages (0-III). Details of changes in the morphology of both the cuticle and the wing epidermal cells are give...
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1016/0040-8166(87)90044-9
更新日期：1987-01-01 00:00:00
abstract：:During the last decade a sophisticated biophysical technique called fluorescence recovery after photobleaching (FRAP) has become available for the measurement of lateral diffusion constants of lipids and proteins in the cell membrane. The information obtainable by this method is of great importance; however, the appli...
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1016/0040-8166(88)90044-4
更新日期：1988-01-01 00:00:00
abstract：:Little is known of the cell biology of Leydig cells during the neonatal activation of the hypothalamic-pituitary-testicular (HPT) axis. The current study examined the effect of blockade of the HPT axis with a GnRH antagonist (antide) on the neonatal population of Leydig cells in the new world primate, the common marmo...
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1016/s0040-8166(98)80084-0
更新日期：1998-12-01 00:00:00
abstract：:We aimed to investigate the application of adipose-derived stromal cells in the treatment of stress urinary incontinence (SUI). Animal models of stress urinary incontinence were established with Sprague-Dawley female rats by complete cutting of the pudendal nerve. Rat adipose-derived stromal cells were isolated, cultu...
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1016/j.tice.2011.04.003
更新日期：2011-08-01 00:00:00
abstract：:Today, regenerative medicine requires new sources of multipotent stem cells for their differentiation to chondrocytes using the mediums of differentiation available in the market. This study aimed to determine whether the Mesenchymal Stem Cells (MSCs) isolated from Mobilized Peripheral Blood (MPB) in sheep using the G...
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1016/j.tice.2018.05.007
更新日期：2018-06-01 00:00:00
abstract：:The intercellular junctions associated with the modified glial cells of the perineurium have been examined in the ganglia and main abdominal nerves of the blood-sucking bug Rhodnius prolixus, both before and and after feeding, by means of freeze-fracture and tracer studies. It was found that the pleated septate juncti...
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1016/0040-8166(85)90040-0
更新日期：1985-01-01 00:00:00
abstract：:Kidney cells of the marine stickleback Spinachia have been studied with histochemical methods for the demonstration of glycoconjugates. The fine structure of epithelial cells is described. Mucus threads in the nephronic tubule of sexually mature consist of neutral glycoprotein which corresponds with the secretory gran...
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1016/0040-8166(79)90060-0
更新日期：1979-01-01 00:00:00
abstract：:In this study, we aimed to investigate the presence of asprosin (ASP) in the liver, kidneys, heart, stomach, testicles and brain and to determine the serum and tissue asprosin levels in diabetic rats. A total of 14 male Wistar Albino rats were divided into two groups, each containing 7 rats: (I) control group and (II)...
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1016/j.tice.2020.101397
更新日期：2020-10-01 00:00:00
abstract：:Intercalated discs are exceptionally complex entities, and possess considerable functional significance in terms of the workings of the myocardium. Examination of different species and heart regions indicates that the original histological term has become out-moded; it is likely, however, that all such complexes will ...
journal_title：Tissue & cell
pub_type： 杂志文章,评审
doi：10.1016/0040-8166(85)90001-1
更新日期：1985-01-01 00:00:00
abstract：:Lipid bodies (LB), lipid-rich inclusions abundantly present in cells engaged in inflammation, are specialized intracellular domains involved in generating inflammatory mediators, the eicosanoids. Since the acute Trypanosoma cruzi infection triggers a potent inflammatory reaction characterized by a great increase of pe...
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1016/s0040-8166(02)00105-2
更新日期：2003-02-01 00:00:00
abstract：:There is now strong evidence that the stem cells of many tissues reside in specialized structures termed niches. The stem cell niche functions to house and regulate symmetric and asymmetric mitosis of stem cells in mammalian skin, mouse and human bone marrow, mouse brain, gut, and hair follicle, and Drosophila ovary a...
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1016/s0040-8166(02)00107-6
更新日期：2003-04-01 00:00:00
abstract：:The Australian lungfish, Neoceratodus forsteri, has a dentition consisting of enamel, mantle dentine and bone, enclosing circumdenteonal, core and interdenteonal dentines. Branching processes from cells that produce interdenteonal dentine leave the cell surface at different angles, with collagen fibrils aligned parall...
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1016/j.tice.2006.01.001
更新日期：2006-04-01 00:00:00
abstract：:Diabetic nephropathy is the common cause of leading to end stage of renal disease (ESRD). Satureja khozestanica essential oil (SKEO) was used as an antioxidant and antidiabetic for the inhibition of diabetic nephropathy. Forty male rats were uninephrectomized and divided in four groups randomly; group one as control, ...
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1016/j.tice.2010.11.004
更新日期：2011-02-01 00:00:00
abstract：:Three different metal salts, silver nitrate, uranyl acetate and lead citrate, are mixed with a collagen gel to produce 3 metal/collagen sponges. These sponges were implanted subcutaneously in the rat and samples harvested after 5 days of implantation. TEM observation shows that sponges are degraded and digested by mac...
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1016/0040-8166(90)90092-n
更新日期：1990-01-01 00:00:00
abstract：:Locust-style and pseudo-orthogonal structures were found in the cuticle of Heteropteryx dilatata, with the tibiae and femora containing both, and the ocular facets showing only helicoidal construction. The same epidermal cells appear to be competent to secrete both locust-style and pseudo-orthogonal cuticle structure....
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1016/0040-8166(92)90103-e
更新日期：1992-01-01 00:00:00
abstract：:The ultrastructure of peduncle muscle attachment to the cuticular flange in the opercular filament of the serpulid Pomatoceros lamarckii Quatrefages is described. The cuticular flange is composed of layers of orthogonally arranged fibres. Specialized epithelial cells (tendon cells) and a collagenous matrix intervene b...
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1016/0040-8166(83)90007-1
更新日期：1983-01-01 00:00:00
abstract：:Fine structural features of Mizuhopecten yessoensis in different embryonic and larval developmental stages from oocyte to early veliger have been studied. Spermatozoa have a conical head, consisting of a short acrosome, nucleus, and 4 mitochondria in the middle piece. The flagellum is approximately 40 μm in length. Oo...
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1016/j.tice.2018.06.002
更新日期：2018-08-01 00:00:00
abstract：:Time lapse cinematography was used to study and compare the proliferation and migration activity of pulmonary endothelial cells and fibroblasts, two cell types with very different structural and functional properties. Endothelial cells were found to have a more rapid growth rate than fibroblasts. Contributing to the s...
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1016/s0040-8166(81)80002-x
更新日期：1981-01-01 00:00:00
abstract：:Using immunoelectronmicroscopy we analyzed qualitative and quantitatively the intracellular distribution of bothropasin, hemorrhagic factor 2 (HF2) and hemorrhagic factor 3 (HF3) in the venom secretory cells from adult snakes in the active (7 days after venom extraction) and in the resting (without venom extraction fo...
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1016/s004081660200068x
更新日期：2002-12-01 00:00:00
abstract：:The aim of the present study was to investigate protein profiles of human umbilical cord blood-derived mesenchymal stem cells (hUCB-MSCs) cultured in normoxic (21% O2) and hypoxic (1% O2) conditions, and evaluate oxygenation effects on angiogenesis in an ischemic hindlimb mouse model using a modified ischemic scoring ...
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1016/j.tice.2017.09.006
更新日期：2017-12-01 00:00:00
abstract：:The number of epithelial cells comprising larval midgut of the tobacco hornworm moth, Manduca sexta increases 200-fold in development from the first to the fifth instar. We have examined larvae periodically before and during molting to follow epithelial cell proliferation and differentiation. The midgut epithelium in ...
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1016/0040-8166(91)90058-2
更新日期：1991-01-01 00:00:00
abstract：:In this study, the olfactory system of a semi-aquatic turtle, the snapping turtle, has been morphologically investigated by electron microscopy, immunohistochemistry, and lectin histochemistry. The nasal cavity of snapping turtle was divided into the upper and lower chambers, lined by the sensory epithelium containing...
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1016/j.tice.2016.03.011
更新日期：2016-06-01 00:00:00
abstract：:The pectoralis major muscle of the pigeon is composed of two types of muscle fibre. In the Type I fibres, the myofibrils are closely packed and there are few mitochondria. The myofibrils in the Type II fibres are separated by numerous columns of large mitochondria and lipid droplets. The membrane systems of the two ty...
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1016/s0040-8166(69)80017-0
更新日期：1969-01-01 00:00:00
abstract：:In the present investigation, we have studied peroxisomes and sterol carrier protein-2 (SCP2) in control and luteinizing hormone stimulated rat luteal cells. Superovulated immature rats in mid-luteal phase (8 days after ovulation) were divided into two groups (n = 4/group) and treated with vehicle (0.2 ml saline), or ...
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1016/s0040-8166(05)80056-4
更新日期：1995-10-01 00:00:00
abstract：:The imaginal antenna of the male silkmoth Antheraea polyphemus is a feather-shaped structure consisting of about 30 flagellomeres, each of which gives off two pairs of side branches. During the pupal stage (lasting for 3 weeks), the antenna develops from a leaf-shaped, flattened epidermal sac ('antennal blade') via tw...
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1016/s0040-8166(95)80048-4
更新日期：1995-06-01 00:00:00
abstract：:Changes in the fine structural organization of the pars intermedia related to the onset of secretory activity within the gland have been studied. It is shown that during the first seven days, following the onset of secretory activity, there is an extensive membraneogenesis within the cytoplasm of the parenchymatous ce...
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1016/s0040-8166(70)80007-6
更新日期：1970-01-01 00:00:00
abstract：:The integument of Euperipatoides leukarti (Onychophora) has been studied by SEM and TEM-histochemical techniques. The thin (2-3 mu) cuticle is essentially arthropodan in design, comprising a thin, lamellate outer epicuticle, a homogeneous inner epicuticle, and an extensive fibrous procuticle. Five tanned lipoprotein l...
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1016/0040-8166(89)90012-8
更新日期：1989-01-01 00:00:00
abstract：:Kupffer's vesicle, a transitory organ peculiar to teleost embryos, was investigated in embryos of Fundulus heteroclitus by scanning electron microscopy. The roof of the vesicle is constructed to rather large columnar cells which are in continuity wiht hypoblast cells. Each of the roof cells is adorned with a single lo...
journal_title：Tissue & cell
pub_type： 杂志文章
doi：10.1016/0040-8166(78)90003-4
更新日期：1978-01-01 00:00:00
