Abstract:
:The umami taste receptor is a heterodimer composed of two members of the T1R taste receptor family: T1R1 and T1R3. The homology models of the ligand binding domains of the human umami receptor have been constructed based on crystallographic structures of the taste receptor of the central nervous system. Furthermore, the molecular simulations of the ligand binding domain show that the likely conformation was that T1R1 protein exists in the closed conformation, and T1R3 in the open conformation in the heterodimer. The molecular docking study of T1R1 and T1R3 in complex with four peptides, including Lys-Gly-Asp-Glu-Ser-Leu-Leu-Ala, Ser-Glu-Glu, G1u-Ser, and Asp-Glu-Ser, displayed that the amino acid residue of SER146 and Glu277 in T1R3 may play great roles in the synergism of umami taste. This docking result further validated the robustness of the model. In the paper, binding of umami peptide and the T1R1/T1R3 receptor was first described and the interaction is the base of umami activity theory.
journal_name
Cell Biochem Biophysjournal_title
Cell biochemistry and biophysicsauthors
Dang Y,Gao X,Xie A,Wu X,Ma Fdoi
10.1007/s12013-014-0141-zsubject
Has Abstractpub_date
2014-12-01 00:00:00pages
1841-8issue
3eissn
1085-9195issn
1559-0283journal_volume
70pub_type
杂志文章abstract::By using a functional approach of reconstituting detergent-solubilized membrane proteins into liposomes and following their function in patch-clamp experiments, we identified a novel mechanosensitive (MS) channel in the thermophilic cell wall-less archaeon Thermoplasma volcanium. Sodium dodecyl sulfate polyacrylamide ...
journal_title:Cell biochemistry and biophysics
pub_type: 杂志文章
doi:10.1385/CBB:34:3:321
更新日期:2001-01-01 00:00:00
abstract::STAT3 pathway plays an important role in the growth of diffuse large B-cell lymphoma (DLBCL) cells. Here we investigated the antitumor activity of Quercetin, a flavonoid compound, in combination with rituximab in DLBCL cell lines in vitro. We found that Quercetin synergistically enhanced rituximab-induced growth inhib...
journal_title:Cell biochemistry and biophysics
pub_type: 杂志文章
doi:10.1007/s12013-014-0064-8
更新日期:2014-11-01 00:00:00
abstract::The study analyzed how enteral ecoimmunonutrition, which comprises probiotics, glutamine, fish oil, and Enteral Nutritional Suspension (TPF), can impact on the enteral permeability and serum Ghrelin activity in severe cerebral stroke patients with lung infection. Among 190 severe cerebral stroke patients with toleranc...
journal_title:Cell biochemistry and biophysics
pub_type: 杂志文章
doi:10.1007/s12013-014-0184-1
更新日期:2015-01-01 00:00:00
abstract::Excessive proliferation of vascular smooth muscle cells is one of the main pathological processes leading to atherosclerosis and intimal hyperplasia after vascular interventional therapy. Our previous study has shown that interferon-γ inducible protein-10 contributes to the proliferation of vascular smooth muscle cell...
journal_title:Cell biochemistry and biophysics
pub_type: 杂志文章
doi:10.1007/s12013-017-0782-9
更新日期:2017-03-01 00:00:00
abstract::The aim of this study was to investigate characteristic CT manifestations of the group of ovarian thecoma-fibroma. 24 patients (26 lesions) presenting with the ovarian thecoma-fibroma were analyzed retrospectively, and the diagnosis were confirmed by pathology after surgery. Our findings included: 22 patients were uni...
journal_title:Cell biochemistry and biophysics
pub_type: 杂志文章
doi:10.1007/s12013-014-0288-7
更新日期:2015-03-01 00:00:00
abstract::There is an emergent need for improving the microsurgical technique of variant arterial anastomosis to reduce the often seen surgery-related complications. We describe in this article our experience in improving this technique, in 73 living donor liver grafts (64 right lobes, 9 left lobes) in patients with end-stage l...
journal_title:Cell biochemistry and biophysics
pub_type: 杂志文章
doi:10.1007/s12013-012-9421-7
更新日期:2013-03-01 00:00:00
abstract::The aim of this study is to investigate whether hemodiafiltration combined with resin-mediated absorption is a better therapy for hyperlipidemic acute pancreatitis. Patients (n = 67) with acute pancreatitis treated in ICU from January 2009 to December 2012 were included in this study. Seven of these 67 cases were diag...
journal_title:Cell biochemistry and biophysics
pub_type: 杂志文章
doi:10.1007/s12013-014-9855-1
更新日期:2014-07-01 00:00:00
abstract::The aim of this study is to examine the efficacy of alprostadil liposomal preparation in the treatment of blue toe syndrome. As many as 32 patients with blue toe syndrome were randomized into the test group and a control group. Patients out of the test group were treated with alprostadil liposomal preparation, while t...
journal_title:Cell biochemistry and biophysics
pub_type: 杂志文章,随机对照试验
doi:10.1007/s12013-014-0448-9
更新日期:2015-05-01 00:00:00
abstract::The overview demonstrates how the use of only one physico-chemical approach, viz., the electron paramagnetic resonance method, allowed detection and identification of dinitrosyl iron complexes with thiol-containing ligands in various animal and bacterial cells. These complexes are formed in biological objects in the p...
journal_title:Cell biochemistry and biophysics
pub_type: 杂志文章,评审
doi:10.1007/s12013-017-0811-8
更新日期:2018-06-01 00:00:00
abstract::Molecular dynamics simulations, binding free energy calculations, principle component analysis (PCA), and residue interaction network analysis were employed in order to investigate the molecular mechanism of M184I single mutation which played pivotal role in making the HIV-1 reverse transcriptase (RT) totally resistan...
journal_title:Cell biochemistry and biophysics
pub_type: 杂志文章
doi:10.1007/s12013-015-0709-2
更新日期:2016-03-01 00:00:00
abstract::Chloride intracellular channel 1 (CLIC1) is a sensor of oxidative stress in endothelial cells (EC). However, the mechanism by which CLIC1 mediate the regulation of endothelial dysfunction has not been established. In this study, overexpressed CLIC1 impaired the ability of the vascular cells to resist oxidative damage ...
journal_title:Cell biochemistry and biophysics
pub_type: 杂志文章
doi:10.1007/s12013-020-00959-6
更新日期:2021-01-11 00:00:00
abstract::Gas chromatography with mass spectrometric detection combined with head-space needle trap extraction as the pre-concentration technique was applied to identify and quantify volatile organic compounds released or metabolised by human umbilical vein endothelial cells. Amongst the consumed species there were eight aldehy...
journal_title:Cell biochemistry and biophysics
pub_type: 杂志文章
doi:10.1007/s12013-014-0201-4
更新日期:2015-01-01 00:00:00
abstract::H5N1 is a subtype of the influenza A virus that can cause disease in humans and many other animal species. Oseltamivir (Tamiflu) is a potent and selective antiviral drug employed to fight the flu virus in infected individuals by inhibiting neuraminidase (NA), a flu protein responsible for the release and spread of the...
journal_title:Cell biochemistry and biophysics
pub_type: 杂志文章
doi:10.1007/s12013-013-9709-2
更新日期:2014-03-01 00:00:00
abstract::Although aggressive therapeutic regimen has been applied in the treatment of Glioblastoma (GBM), the prognosis of patients with GBM remains poor. Preclinical studies have demonstrated the efficacy of Suntinib in GBM both in vitro and in vivo. In this study, we showed that the cytotoxicity was enhanced by transfection ...
journal_title:Cell biochemistry and biophysics
pub_type: 杂志文章
doi:10.1007/s12013-014-0501-8
更新日期:2015-06-01 00:00:00
abstract::Behcet's disease (BD) accompanied by intestinal involvement is called intestinal BD. Although recent studies have attained positive feedback with the administration of anti-TNF-α agents in patients with BD, only a few reports on the study of etanercept in intestinal BD have been found. In this study, 35 cases of intes...
journal_title:Cell biochemistry and biophysics
pub_type: 杂志文章
doi:10.1007/s12013-014-9860-4
更新日期:2014-07-01 00:00:00
abstract::Human copper transporter 1 (hCTR1) is the major high affinity copper influx transporter in mammalian cells that also mediates uptake of the cancer chemotherapeutic agent cisplatin. A low resolution structure of hCTR1 determined by cryoelectron microscopy was recently published. Several protein structure simulation tec...
journal_title:Cell biochemistry and biophysics
pub_type: 杂志文章
doi:10.1007/s12013-012-9358-x
更新日期:2012-07-01 00:00:00
abstract::Leptin is a protein hormone which plays a critical role in the regulation of both body-weight through reducing food intake and stimulating energy expenditure. Several polymorphisms in leptin gene (LEP), which encodes for leptin, have been described. However, its association with obesity is still controversial. Therefo...
journal_title:Cell biochemistry and biophysics
pub_type: 杂志文章
doi:10.1007/s12013-012-9427-1
更新日期:2013-03-01 00:00:00
abstract::Early events in individual hepatocytes of rat activated by adrenaline (10(-6)M) and phenylephrine (10(-5)M) have been investigated by quantitative image microfluorometry and microspectrofluorometry. Cationic DiOC2 and anionic SqSC4 probes have been used for image analysis and transmembrane potential (ΔΨ p) estimation ...
journal_title:Cell biochemistry and biophysics
pub_type: 杂志文章
doi:10.1007/s12013-013-9569-9
更新日期:2013-11-01 00:00:00
abstract::To investigate the effects of the HDMX polymorphism on sarcoma risk. Relevant studies were identified by searching the PubMed, Embase, and Web of Science databases. Data were extracted by two independent investigators. Odds ratios (ORs) and 95 % confidence intervals (CIs) were calculated using a fixed-effects model to...
journal_title:Cell biochemistry and biophysics
pub_type: 杂志文章,meta分析
doi:10.1007/s12013-014-0111-5
更新日期:2014-12-01 00:00:00
abstract::The American Diabetes Association announces funding for clinical and basic science studies in three major areas of research that focus on cell-based insulin replacement in type 1 diabetes: 1. Genetic engineering of nonpancreatic cells into glucose-sensitive insulin-producing cells; 2. Transforming stem cells, pancreat...
journal_title:Cell biochemistry and biophysics
pub_type: 杂志文章
doi:10.1385/cbb:40:3s:023
更新日期:2004-01-01 00:00:00
abstract::The objective of this study is to investigate the roles of acteoside (ACT) in cells with oxygen-glucose deprivation and reoxygenation (OGD/R)-induced injury and the underlying mechanisms. The differentially expressed genes (DEGs) in rats with middle cerebral artery occlusion were identified using GSE61616 data set. Ky...
journal_title:Cell biochemistry and biophysics
pub_type: 杂志文章
doi:10.1007/s12013-020-00965-8
更新日期:2021-01-13 00:00:00
abstract::We wished to study the efficacy and safety of the retrograde ligation of short hepatic veins (SHVs) and the right hepatic vein (HV) through the retrohepatic tunnel in patients who underwent hemihepatectomy due to large hepatic carcinoma in the right lobe of the liver. Right hemihepatectomy was performed in 23 patients...
journal_title:Cell biochemistry and biophysics
pub_type: 临床试验,杂志文章
doi:10.1007/s12013-011-9268-3
更新日期:2012-01-01 00:00:00
abstract::In the present study, the changes of the regulatory T cells (Treg) expression, endotoxin translocation, and the relationships in intestinal lymph nodes were investigated in porcine sepsis induced by severe burns. Flow cytometry, western blot, and Tachypleus amebocyte lysate were applied to study after the burn injury ...
journal_title:Cell biochemistry and biophysics
pub_type: 杂志文章
doi:10.1007/s12013-014-0116-0
更新日期:2014-12-01 00:00:00
abstract::Leukocyte transendothelial migration is a multistep process coordinated by chemokine receptors, integrins and cell adhesion molecules. The interaction between leukocytes and endothelial cells is accompanied by bidirectional signaling in both cell types, which is initiated following formation of specialized, "docking" ...
journal_title:Cell biochemistry and biophysics
pub_type: 杂志文章,评审
doi:10.1385/cbb:38:3:305
更新日期:2003-01-01 00:00:00
abstract::Non-steroidal anti-inflammatory drugs (NSAIDs) are frequently used to alleviate pain of the patients who suffer from inflammatory conditions like rheumatoid arthritis, osteoarthritis, and other painful conditions like gout. This class of drugs works by blocking cyclooxgenases which in turn block the prostaglandin prod...
journal_title:Cell biochemistry and biophysics
pub_type: 杂志文章,评审
doi:10.1007/s12013-013-9791-5
更新日期:2014-06-01 00:00:00
abstract::The mechanism of induction of cardiomyocyte (CM) dedifferentiation, as seen in chronic hibernating myocardium, is largely unknown. Recently, a cellular model was proposed consisting of long-term cocultures of adult rabbit CMs and cardiac fibroblasts in which typical structural characteristics of hibernation-like dedif...
journal_title:Cell biochemistry and biophysics
pub_type: 杂志文章
doi:10.1385/CBB:44:1:119
更新日期:2006-01-01 00:00:00
abstract::Non-esterified fatty acids (NEFAs) act as signaling molecules involved in regulating genes expression to modulate lipid metabolism. However, the regulation mechanism of NEFAs on lipid metabolism in dairy cows is unclear. The AMP-activated protein kinase (AMPK) signaling pathway plays a key role in regulating hepatic l...
journal_title:Cell biochemistry and biophysics
pub_type: 杂志文章
doi:10.1007/s12013-013-9629-1
更新日期:2013-01-01 00:00:00
abstract::To investigate if the 12-lead resting electrocardiogram (ECG) is a predictor of left ventricular (LV) functional recovery after revascularization of chronic total coronary artery occlusions (CTO). Revascularization was performed in 58 CTO patients who had impaired regional wall motion. The 12-lead resting ECG was used...
journal_title:Cell biochemistry and biophysics
pub_type: 杂志文章
doi:10.1007/s12013-014-9862-2
更新日期:2014-09-01 00:00:00
abstract::Cellular responses to external signals often reflect alterations in gene expression. The activation of cell surface hormone or growth factor receptors upon the binding of appropriate ligands mobilizes signal transduction cascades that can ultimately impact the activity of defined sets of transcription factors. The int...
journal_title:Cell biochemistry and biophysics
pub_type: 杂志文章,评审
doi:10.1007/BF02737882
更新日期:1999-01-01 00:00:00
abstract::Epithelial ovarian cancer (EOC) is the second leading cause of death from gynecological malignancies worldwide. Enhancer of zeste homology 2 (EZH2), participating in gene expression silencing by trimethylating histone 3 lysine 27 (H3K27me3), is often up-regulated in EOC. ARHI, an imprinted tumor-suppressor gene, is ma...
journal_title:Cell biochemistry and biophysics
pub_type: 杂志文章
doi:10.1007/s12013-014-0168-1
更新日期:2015-01-01 00:00:00