Sema4D knockdown in oligodendrocytes promotes functional recovery after spinal cord injury.

Abstract:

:Semaphorin4D (Sema4D) belongs to Semaphorins family and is secreted and membrane-bound protein. Its function on angiogenesis and axon regeneration makes it an ideal therapeutic target for spinal cord injury (SCI). Here we examined Sema4D expression profile by real-time PCR and western blot and found Sema4D was upregulated after SCI. In vitro study showed Sema4D was not only expressed in oligodendrocytes but also in endothelial cells (ECs). Hypoxia can mimic Sema4D upregulation in both cell lines. Moreover, overexpression of Sema4D through lentivirus in ECs promoted tube formation. However, Sema4D overexpression in oligodendrocytes precursor cells (OPCs) inhibited neuron myelination in neuron-oligodendrocyte co-culture system. Therefore, Sema4D knockdown in OPCs was applied in SCI rats. The results indicated that Sema4D knockdown significantly promoted functional recovery with blood-brain barrier score. Taken together, our data suggest that specific Sema4D knockdown in oligodendrocytes without disturbing its angiogenesis effect can be a beneficial strategy for SCI treatment.

journal_name

Cell Biochem Biophys

authors

Zhang HL,Wang J,Tang L

doi

10.1007/s12013-013-9727-0

subject

Has Abstract

pub_date

2014-04-01 00:00:00

pages

489-96

issue

3

eissn

1085-9195

issn

1559-0283

journal_volume

68

pub_type

杂志文章
  • Chemopreventive Effects of Azadirachta indica on Cancer Marker Indices and Ultrastructural Changes During 1,2-Dimethylhydrazine-Induced Colon Carcinogenesis in Rats.

    abstract::The present study elucidated the prospective of Azadirachta indica supplementation, if any, in affording chemoprevention by modulating the altered cancer markers and ultrastructural changes in DMH-induced colorectal carcinogenesis in rats. The rats were segregated into four groups viz., normal control, DMH treated, A....

    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章

    doi:10.1007/s12013-015-0621-9

    authors: Liu N,Sun B,Wu P,Wei X

    更新日期:2015-09-01 00:00:00

  • Analysis of Myocardial (18)F-FDG Uptake by PET/CT in the Patients with Different Dialectically Classified Coronary Heart Diseases (CHD).

    abstract::To quantify myocardial glucose metabolism by (18)F-FDG PET/CT in patients that have coronary heart disease (CHD) according to traditional Chinese medicine classification. Ninety patients with CHD were enrolled and were categorized into three groups. All patients underwent PET-CT examination for (18)F-FDG uptake quanti...

    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章

    doi:10.1007/s12013-015-0538-3

    authors: Tan YY,Liu DF,Zhu F,Ding XM,Wang GM

    更新日期:2015-07-01 00:00:00

  • Regulation of tetrahydrobiopterin synthesis and bioavailability in endothelial cells.

    abstract::Tetrahydrobiopterin (BH4) is a member of the pterin family that has a core structure of pyrazino-2,3-d-pyrimidine rings. Because BH4 is an essential cofactor for the biosynthesis of nitric oxide (a major vasodilator), there is growing interest in BH4 biochemistry in endothelial cells (the cells that line blood vessels...

    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章,评审

    doi:10.1385/CBB:41:3:415

    authors: Shi W,Meininger CJ,Haynes TE,Hatakeyama K,Wu G

    更新日期:2004-01-01 00:00:00

  • Electrical inhomogeneity in left ventricular hypertrophy.

    abstract::Recent studies designed to assess the relationship between aortic compliance and heterogeneity of heart electrical activity has shown that hypertrophy aggravates repolarization disturbances in the myocardium. Numerous mechanisms of electrical instability and inhomogeneity associated with left ventricular hypertrophy a...

    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章,评审

    doi:10.1007/s12013-014-9850-6

    authors: Gao C,Yang D

    更新日期:2014-07-01 00:00:00

  • Effects of Curcumin on Lipid Membranes: an EPR Spin-label Study.

    abstract::Curcumin is a yellow-orange dye widely used as a spice, food coloring and food preservative. It also exhibits a broad range of therapeutic effects against different disorders such as cancer, diabetes, or neurodegenerative diseases. As a compound insoluble in water curcumin accumulates in cell membranes and due to this...

    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章

    doi:10.1007/s12013-020-00906-5

    authors: Duda M,Cygan K,Wisniewska-Becker A

    更新日期:2020-06-01 00:00:00

  • Request for applications: islet cell replacement in type 1 diabetes.

    abstract::The American Diabetes Association announces funding for clinical and basic science studies in three major areas of research that focus on cell-based insulin replacement in type 1 diabetes: 1. Genetic engineering of nonpancreatic cells into glucose-sensitive insulin-producing cells; 2. Transforming stem cells, pancreat...

    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章

    doi:10.1385/cbb:40:3s:023

    authors: Campbell S

    更新日期:2004-01-01 00:00:00

  • Phenotype switch of vascular smooth muscle cells after siRNA silencing of filamin.

    abstract::Filamin (FLN) plays an important role in differentiation, migration, and signal transduction in vascular smooth muscle cells (VSMCs). We hypothesized that suppression of FLN expression would inhibit differentiation and migration of VSMCs, and interrupt the phenotype switch of these cells. We designed and tested differ...

    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章

    doi:10.1007/s12013-011-9159-7

    authors: Zhu G,Chen H,Zhang W

    更新日期:2011-09-01 00:00:00

  • Determination of concentration of methotrexate enantiomers in intracellular and extracellular fluids of HepG2 cells by liquid chromatography-tandem mass spectrometry.

    abstract::A rapid and sensitive liquid chromatography-tandem mass spectrometry assay (LC-MS/MS) with electrospray ionization was developed and validated for the quantitative determination of the concentration of methotrexate (MTX) enantiomers in intracellular and extracellular fluids of HepG2 cells. The analytes were extracted ...

    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章

    doi:10.1007/s12013-013-9666-9

    authors: Wang R,Guo L,Xie H,Zhang J,Li X,Li W,Wang J,Wu X,Jia Z

    更新日期:2013-01-01 00:00:00

  • Comparison of the Outcomes of Two Operational Methods Used for the Fixation of Calcaneal Fracture.

    abstract::This study was aimed to compare the outcomes of two operational methods used for the fixation of calcaneal fracture, the open reduction using a plate and the minimally invasive cannulated screw fixations. Thus, we attempted to find out as to which of these fixation regimens was therapeutically superior by assessing im...

    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章,随机对照试验

    doi:10.1007/s12013-014-0436-0

    authors: Wang Q,Li X,Sun Y,Yan L,Xiong C,Wang J

    更新日期:2015-05-01 00:00:00

  • Deciphering Biophysical Modulation in Ovarian Cancer Cells.

    abstract::It has been long known that the oncogenic extracellular environment plays an indispensable role in developing and nurturing cancer cell progression and in resistance to standard treatments. However, by how much the biophysical components of tumour extracellular environment contribute to these processes is uncertain. I...

    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章

    doi:10.1007/s12013-020-00964-9

    authors: Sarwar M,Sykes PH,Chitcholtan K,Evans JJ

    更新日期:2021-01-12 00:00:00

  • Simultaneous determination of calycosin-7-O-β-D-glucoside, ononin, calycosin, formononetin, astragaloside IV, and astragaloside II in rat plasma after oral administration of Radix Astragali extraction for their pharmacokinetic studies by ultra-pressure li

    abstract::A sensitive and reliable ultra-pressure liquid chromatography with tandem mass spectrometry (UPLC-MS) was developed and validated for simultaneous quantification of six main bioactive components, i.e., calycosin-7-O-β-D-glucoside, ononin, calycosin, formononetin, astragaloside IV, and astragaloside II in rat plasma af...

    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章

    doi:10.1007/s12013-014-9972-x

    authors: Liu XH,Zhao JB,Guo L,Yang YL,Hu F,Zhu RJ,Feng SL

    更新日期:2014-09-01 00:00:00

  • Advantages, Disadvantages, and Trend of Integrative Medicine in the Treatment of Heart Failure.

    abstract::Integrative medicine therapy using traditional Chinese medicine (TCM) combined with western medicine has shown some advantages in treating heart failure (HF), such as holistic concept; multi-target treatment; dialectical logic; personalized therapy; formulae compatibility; and reduction of side effects of western medi...

    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章,评审

    doi:10.1007/s12013-014-0466-7

    authors: Zhang P

    更新日期:2015-06-01 00:00:00

  • Fibrin-sealant-delivered cisplatin chemotherapy versus cisplatin hyperthermic intraperitoneal perfusion chemotherapy for locally advanced gastric cancer without peritoneal metastases: a randomized phase-II clinical trial with a 40-month follow-up.

    abstract::A new intraoperative cisplatin administration method for patients with locally advanced gastric cancer (AGC) and without peritoneal metastasis, fibrin-sealant-delivered cisplatin chemotherapy, was reported, and its safety, pharmacokinetics, and efficacy were compared with cisplatin hyperthermic intraperitoneal perfusi...

    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章,随机对照试验

    doi:10.1007/s12013-014-0326-5

    authors: Huang O,Lu X,Xu X,Shi Y

    更新日期:2015-03-01 00:00:00

  • Differential effects of G- and F-actin on the plasma membrane calcium pump activity.

    abstract::We have previously shown that plasma membrane calcium ATPase (PMCA) pump activity is affected by the membrane protein concentration (Vanagas et al., Biochim Biophys Acta 1768:1641-1644, 2007). The results of this study provided evidence for the involvement of the actin cytoskeleton. In this study, we explored the rela...

    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章

    doi:10.1007/s12013-012-9467-6

    authors: Vanagas L,de La Fuente MC,Dalghi M,Ferreira-Gomes M,Rossi RC,Strehler EE,Mangialavori IC,Rossi JP

    更新日期:2013-05-01 00:00:00

  • Therapeutical effects and mechanism of salubrinal combined with ulinastatin on treating paraquat poisoning.

    abstract::To explore therapeutic effects and underlying mechanism of Salubrinal combined with Ulinastatin (UTI) on acute Paraquat (PQ) poisoning. Four hundred rats were randomly allocated into UTI group, SAL group, SAL + UTI and control group according to random number table with 100 rats in each group. Acute PQ poisoning model...

    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章

    doi:10.1007/s12013-014-0095-1

    authors: Guo H,Jiang C,Sun X

    更新日期:2014-12-01 00:00:00

  • Cell contraction caused by microtubule disruption is accompanied by shape changes and an increased elasticity measured by scanning acoustic microscopy.

    abstract::The state of crosslinking of microfilaments and the state of myosin-driven contraction are the main determinants of the mechanical properties of the cell cortex underneath the membrane, which is significant for the mechanism of shaping cells. Therefore, any change in the contractile state of the actomyosin network wou...

    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章

    doi:10.1007/BF02737896

    authors: Karl I,Bereiter-Hahn J

    更新日期:1998-01-01 00:00:00

  • Ion exchangers mediating NaCl transport in the renal proximal tubule.

    abstract::We have studied the mechanisms of NaCl transport in the mammalian proximal tubule. Studies of isolated brush-border membrane vesicles confirmed the presence of Na+-H+ exchange and identified Cl(-)-formate and Cl(-)-oxalate exchangers as possible mechanisms of uphill Cl- entry. We found that formate and oxalate each st...

    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章,评审

    doi:10.1385/CBB:36:2-3:147

    authors: Aronson PS

    更新日期:2002-01-01 00:00:00

  • Posterior fossa decompression combined with resection of the cerebellomedullary fissure membrane and expansile duraplasty: a radical and rational surgical treatment for Arnold-Chiari type I malformation.

    abstract::This study aims to introduce a new surgical procedure for the treatment of Arnold-Chiari type I malformation (ACM-1) and to compare its effectiveness with the techniques described in previous studies. We performed the following procedures: foramen magnum decompression combined with the removal of both the atlanto-occi...

    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章

    doi:10.1007/s12013-014-0135-x

    authors: Liang CJ,Dong QJ,Xing YH,Shan M,Wen LX,Qiang ZY,Ping ZQ,Tao PZ,Ping HX

    更新日期:2014-12-01 00:00:00

  • Influence of Val108/158Met COMT Gene Polymorphism on the Efficacy of Modified Electroconvulsive Therapy in Patients with Treatment Resistant Depression.

    abstract::Depression is a common emotional disorder associated with increased risk of suicide and rate of disability. In this double-blinded control study, we tested the efficacy of modified electroconvulsive therapy (MECT) in patients with treatment resistant depression (TRD) using the Hamilton Depression Rating Scale for Depr...

    journal_title:Cell biochemistry and biophysics

    pub_type: 临床试验,杂志文章

    doi:10.1007/s12013-014-0361-2

    authors: Lin Z,He H,Zhang C,Wang Z,Jiang M,Li Q,Lan X,Zhang M,Huang X

    更新日期:2015-04-01 00:00:00

  • Glycogen debranching enzyme 6 (AGL), enolase 1 (ENOSF1), ectonucleotide pyrophosphatase 2 (ENPP2_1), glutathione S-transferase 3 (GSTM3_3) and mannosidase (MAN2B2) metabolism computational network analysis between chimpanzee and human left cerebrum.

    abstract::We identified significantly higher expression of the genes glycogen debranching enzyme 6 (AGL), enolase 1 (ENOSF1), ectonucleotide pyrophosphatase 2 (ENPP2_1), glutathione S-transferase 3 (GSTM3_3) and mannosidase (MAN2B2) from human left cerebrums versus chimpanzees. Yet the distinct low- and high-expression AGL, ENO...

    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章

    doi:10.1007/s12013-011-9232-2

    authors: Sun L,Wang L,Jiang M,Huang J,Lin H

    更新日期:2011-12-01 00:00:00

  • Evaluating spatial constraints in cellular assembly processes using a monte carlo approach.

    abstract::Biomolecular behavior commonly involves complex sets of interacting components that are challenging to understand through solution-based chemical theories. Molecular assembly is especially intriguing in the cellular environment because of its links to cell structure in processes such as chemotaxis. We use a coarse-gra...

    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章

    doi:10.1385/cbb:45:2:195

    authors: Puskar K,Ta 'asan S,Schwartz R,LeDuc PR

    更新日期:2006-01-01 00:00:00

  • Spectral CT imaging in patients with Budd-Chiari syndrome: investigation of image quality.

    abstract::To assess the image quality of monochromatic imaging from spectral CT in patients with Budd-Chiari syndrome (BCS), fifty patients with BCS underwent spectral CT to generate conventional 140 kVp polychromatic images (group A) and monochromatic images, with energy levels from 40 to 80, 40 + 70, and 50 + 70 keV fusion im...

    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章

    doi:10.1007/s12013-014-0021-6

    authors: Su L,Dong J,Sun Q,Liu J,Lv P,Hu L,Yan L,Gao J

    更新日期:2014-11-01 00:00:00

  • A Simple Analytical Approximation to an Inhomogeneously-Broadened Dispersion Spectrum. Application to Absorption-Dispersion Admixtures.

    abstract::A simple analytical approximation to an inhomogeneously-broadened dispersion signal is proposed and tested with resonance lines broadened by unresolved hyperfine structure. Spectral parameters may be rapidly and accurately extracted using a nonlinear least-squares fitting algorithm. Combining the new approximation to ...

    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章

    doi:10.1007/s12013-016-0760-7

    authors: Bales BL

    更新日期:2017-06-01 00:00:00

  • Better prognosis in newborns with trisomy 13 who received intensive treatments: a retrospective study of 16 patients.

    abstract::Intensive treatment for newborns with trisomy 13 is controversial because of their lethal prognosis. We report the better life prognosis of patients with trisomy 13 who received intensive treatment. At our hospital, we provided an intensive management to such patients including resuscitation and surgical procedures as...

    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章

    doi:10.1007/s12013-012-9355-0

    authors: Tsukada K,Imataka G,Suzumura H,Arisaka O

    更新日期:2012-07-01 00:00:00

  • The Use of Calcium Channel Blockers in the Treatment of Coronary Spasm and Atrioventricular Block.

    abstract::Calcium channel blockers have been used in the treatment of coronary artery spasm for many years. However, there is insufficient knowledge about their application to treat atrioventricular block caused by coronary spasm. Clinical data of five patients who were diagnosed with coronary spasm caused by atrioventricular b...

    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章

    doi:10.1007/s12013-014-0498-z

    authors: Gao B,Zhang Z,Qian J,Cao C,Hua X,Chu M,He X,Zeng H

    更新日期:2015-06-01 00:00:00

  • Quercetin potentiates the antitumor activity of rituximab in diffuse large B-cell lymphoma by inhibiting STAT3 pathway.

    abstract::STAT3 pathway plays an important role in the growth of diffuse large B-cell lymphoma (DLBCL) cells. Here we investigated the antitumor activity of Quercetin, a flavonoid compound, in combination with rituximab in DLBCL cell lines in vitro. We found that Quercetin synergistically enhanced rituximab-induced growth inhib...

    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章

    doi:10.1007/s12013-014-0064-8

    authors: Li X,Wang X,Zhang M,Li A,Sun Z,Yu Q

    更新日期:2014-11-01 00:00:00

  • Molecular mechanisms by which saturated fatty acids modulate TNF-α expression in mouse macrophage lineage.

    abstract::Many macrophage functions are modulated by fatty acids (FAs), including cytokine release, such as tumor necrosis factor-α (TNF-α). TNF-α is of great interest due to its role in the inflammation process observed in several diseases such as rheumatoid arthritis, atherosclerosis, and obesity. However, the mechanisms by w...

    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章

    doi:10.1007/s12013-010-9117-9

    authors: de Lima-Salgado TM,Alba-Loureiro TC,do Nascimento CS,Nunes MT,Curi R

    更新日期:2011-03-01 00:00:00

  • Coating and selective deposition of nanofilm on silicone rubber for cell adhesion and growth.

    abstract::A recently developed method for surface modification, layer-by-layer (LbL) assembly, has been applied to silicone, and its ability to encourage endothelial cell growth and control cell growth patterns has been examined. The surfaces studied consisted of a precursor, with alternating cationic polyethyleneimine (PEI) an...

    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章

    doi:10.1385/CBB:38:2:103

    authors: Ai H,Lvov YM,Mills DK,Jennings M,Alexander JS,Jones SA

    更新日期:2003-01-01 00:00:00

  • Identification of unknown homeopathic remedies by delayed luminescence.

    abstract::A quality control method of highly diluted and potentized homeopathic remedies is important for curing patients applying homeopathic therapy. Lenger detected photons in highly potentized homeopathic remedies by delayed luminescence. The photons of Argentum metallicum 100MK and Cantharis 100MK magnetically bound to the...

    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章

    doi:10.1007/s12013-013-9712-7

    authors: Lenger K,Bajpai RP,Spielmann M

    更新日期:2014-03-01 00:00:00

  • An observational study of autologous bone marrow-derived stem cells transplantation in seven patients with nervous system diseases: a 2-year follow-up.

    abstract::Currently, autologous bone marrow-derived stem cell is one of the most innovative areas of stem cells research. Previous studies on animal models of nervous system diseases have shown that these cells have a good effect on nervous system disorders. The alternative treatment with stem cells for the nervous system disea...

    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章

    doi:10.1007/s12013-013-9756-8

    authors: Ren C,Geng RL,Ge W,Liu XY,Chen H,Wan MR,Geng DQ

    更新日期:2014-05-01 00:00:00