Sema4D knockdown in oligodendrocytes promotes functional recovery after spinal cord injury.


:Semaphorin4D (Sema4D) belongs to Semaphorins family and is secreted and membrane-bound protein. Its function on angiogenesis and axon regeneration makes it an ideal therapeutic target for spinal cord injury (SCI). Here we examined Sema4D expression profile by real-time PCR and western blot and found Sema4D was upregulated after SCI. In vitro study showed Sema4D was not only expressed in oligodendrocytes but also in endothelial cells (ECs). Hypoxia can mimic Sema4D upregulation in both cell lines. Moreover, overexpression of Sema4D through lentivirus in ECs promoted tube formation. However, Sema4D overexpression in oligodendrocytes precursor cells (OPCs) inhibited neuron myelination in neuron-oligodendrocyte co-culture system. Therefore, Sema4D knockdown in OPCs was applied in SCI rats. The results indicated that Sema4D knockdown significantly promoted functional recovery with blood-brain barrier score. Taken together, our data suggest that specific Sema4D knockdown in oligodendrocytes without disturbing its angiogenesis effect can be a beneficial strategy for SCI treatment.


Cell Biochem Biophys


Zhang HL,Wang J,Tang L




Has Abstract


2014-04-01 00:00:00












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    abstract::The present study elucidated the prospective of Azadirachta indica supplementation, if any, in affording chemoprevention by modulating the altered cancer markers and ultrastructural changes in DMH-induced colorectal carcinogenesis in rats. The rats were segregated into four groups viz., normal control, DMH treated, A....

    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章


    authors: Liu N,Sun B,Wu P,Wei X

    更新日期:2015-09-01 00:00:00

  • Analysis of Myocardial (18)F-FDG Uptake by PET/CT in the Patients with Different Dialectically Classified Coronary Heart Diseases (CHD).

    abstract::To quantify myocardial glucose metabolism by (18)F-FDG PET/CT in patients that have coronary heart disease (CHD) according to traditional Chinese medicine classification. Ninety patients with CHD were enrolled and were categorized into three groups. All patients underwent PET-CT examination for (18)F-FDG uptake quanti...

    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章


    authors: Tan YY,Liu DF,Zhu F,Ding XM,Wang GM

    更新日期:2015-07-01 00:00:00

  • Regulation of tetrahydrobiopterin synthesis and bioavailability in endothelial cells.

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    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章,评审


    authors: Shi W,Meininger CJ,Haynes TE,Hatakeyama K,Wu G

    更新日期:2004-01-01 00:00:00

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    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章,评审


    authors: Gao C,Yang D

    更新日期:2014-07-01 00:00:00

  • Effects of Curcumin on Lipid Membranes: an EPR Spin-label Study.

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    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章


    authors: Duda M,Cygan K,Wisniewska-Becker A

    更新日期:2020-06-01 00:00:00

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    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章


    authors: Campbell S

    更新日期:2004-01-01 00:00:00

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    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章


    authors: Zhu G,Chen H,Zhang W

    更新日期:2011-09-01 00:00:00

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    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章


    authors: Wang R,Guo L,Xie H,Zhang J,Li X,Li W,Wang J,Wu X,Jia Z

    更新日期:2013-01-01 00:00:00

  • Comparison of the Outcomes of Two Operational Methods Used for the Fixation of Calcaneal Fracture.

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    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章,随机对照试验


    authors: Wang Q,Li X,Sun Y,Yan L,Xiong C,Wang J

    更新日期:2015-05-01 00:00:00

  • Deciphering Biophysical Modulation in Ovarian Cancer Cells.

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    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章


    authors: Sarwar M,Sykes PH,Chitcholtan K,Evans JJ

    更新日期:2021-01-12 00:00:00

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    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章


    authors: Liu XH,Zhao JB,Guo L,Yang YL,Hu F,Zhu RJ,Feng SL

    更新日期:2014-09-01 00:00:00

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    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章,评审


    authors: Zhang P

    更新日期:2015-06-01 00:00:00

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    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章,随机对照试验


    authors: Huang O,Lu X,Xu X,Shi Y

    更新日期:2015-03-01 00:00:00

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    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章


    authors: Vanagas L,de La Fuente MC,Dalghi M,Ferreira-Gomes M,Rossi RC,Strehler EE,Mangialavori IC,Rossi JP

    更新日期:2013-05-01 00:00:00

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    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章


    authors: Guo H,Jiang C,Sun X

    更新日期:2014-12-01 00:00:00

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    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章


    authors: Karl I,Bereiter-Hahn J

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    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章,评审


    authors: Aronson PS

    更新日期:2002-01-01 00:00:00

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    journal_title:Cell biochemistry and biophysics

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    authors: Liang CJ,Dong QJ,Xing YH,Shan M,Wen LX,Qiang ZY,Ping ZQ,Tao PZ,Ping HX

    更新日期:2014-12-01 00:00:00

  • Influence of Val108/158Met COMT Gene Polymorphism on the Efficacy of Modified Electroconvulsive Therapy in Patients with Treatment Resistant Depression.

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    journal_title:Cell biochemistry and biophysics

    pub_type: 临床试验,杂志文章


    authors: Lin Z,He H,Zhang C,Wang Z,Jiang M,Li Q,Lan X,Zhang M,Huang X

    更新日期:2015-04-01 00:00:00

  • Glycogen debranching enzyme 6 (AGL), enolase 1 (ENOSF1), ectonucleotide pyrophosphatase 2 (ENPP2_1), glutathione S-transferase 3 (GSTM3_3) and mannosidase (MAN2B2) metabolism computational network analysis between chimpanzee and human left cerebrum.

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    journal_title:Cell biochemistry and biophysics

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    authors: Sun L,Wang L,Jiang M,Huang J,Lin H

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    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章


    authors: Puskar K,Ta 'asan S,Schwartz R,LeDuc PR

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  • Spectral CT imaging in patients with Budd-Chiari syndrome: investigation of image quality.

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    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章


    authors: Su L,Dong J,Sun Q,Liu J,Lv P,Hu L,Yan L,Gao J

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    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章


    authors: Bales BL

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    journal_title:Cell biochemistry and biophysics

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    authors: Tsukada K,Imataka G,Suzumura H,Arisaka O

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    journal_title:Cell biochemistry and biophysics

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    authors: Gao B,Zhang Z,Qian J,Cao C,Hua X,Chu M,He X,Zeng H

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    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章


    authors: Li X,Wang X,Zhang M,Li A,Sun Z,Yu Q

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    journal_title:Cell biochemistry and biophysics

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    authors: de Lima-Salgado TM,Alba-Loureiro TC,do Nascimento CS,Nunes MT,Curi R

    更新日期:2011-03-01 00:00:00

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    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章


    authors: Ai H,Lvov YM,Mills DK,Jennings M,Alexander JS,Jones SA

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    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章


    authors: Lenger K,Bajpai RP,Spielmann M

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    journal_title:Cell biochemistry and biophysics

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    authors: Ren C,Geng RL,Ge W,Liu XY,Chen H,Wan MR,Geng DQ

    更新日期:2014-05-01 00:00:00