Abstract：
:The present study aimed at detecting DNA damage and fragmentation as well as histone acetylation depending on oxidative stress caused by CCl4 intoxication. Also, the protective role of N-acetyl cysteine, a precursor for GSH, in DNA damage is investigated. Sixty rats were used in this study. In order to induce liver toxicity, CCl4 in was dissolved in olive oil (1/1) and injected intraperitoneally as a single dose (2 ml/kg). N-acetyl cysteine application (intraperitoneal, 50 mg/kg/day) was started 3 days prior to CCl4 injection and continued during the experimental period. Control groups were given olive oil and N-acetyl cysteine. After 6 and 72 h of CCl4 injection, blood and liver tissue were taken under ether anesthesia. Nuclear extracts were prepared from liver. Changes in serum AST and ALT activities as well as MDA, TAS, and TOS levels showed that CCl4 caused lipid peroxidation and liver damage. However, lipid peroxidation and liver damage were reduced in the N-acetyl cysteine group. Increased levels in 8-hydroxy-2-deoxy guanosine and histone acetyltransferase activities, decreased histone deacetylase activities, and DNA breakage detected in nuclear extracts showed that CCl4 intoxication induces oxidative stress and apoptosis in rat liver. The results of the present study indicate that N-acetyl cysteine has a protective effect on CCl4-induced DNA damage.
journal_name
Cell Biochem Biophysjournal_title
Cell biochemistry and biophysicsauthors
Aksit H,Bildik Adoi
10.1007/s12013-014-0031-4subject
Has Abstractpub_date
2014-11-01 00:00:00pages
1119-25issue
2eissn
1085-9195issn
1559-0283journal_volume
70pub_type
杂志文章abstract：:The objective of this study was to evaluate the protective effect of recombinant human brain natriuretic peptide (rhBNP) on endotoxin-induced acute kidney injury (AKI) in canine model of septic shock and its potential mechanisms. Dogs with endotoxin-induced septic shock were subjected to intravenous infusion of saline...
journal_title：Cell biochemistry and biophysics
pub_type： 杂志文章
doi：10.1007/s12013-014-0057-7
更新日期：2014-11-01 00:00:00
abstract：:Na,K-ATPase (EC 3.6.1.37, Na,K-ATPase) is a fundamental vital membrane transport and receptor system which, after biosynthesis, is exported to the plasma membrane in inside-out vesicles. Na,K-ATPase can be extracted form the natural membrane and inserted into artificially formed phosphatidylcholine vesicles (liposomes...
journal_title：Cell biochemistry and biophysics
pub_type： 杂志文章
doi：10.1007/BF02738123
更新日期：1999-01-01 00:00:00
abstract：:Myocardium viability in ischemic heart failure (HF) may affect the effect of cardiac resynchronization therapy (CRT). We hypothesized that longitudinal strain of 2D-STE, which reflects myocardium viability, can predict the response to CRT in patients with ischemic HF. 2D-STE was performed in 42 patients with HF, 1 wee...
journal_title：Cell biochemistry and biophysics
pub_type： 杂志文章
doi：10.1007/s12013-014-9870-2
更新日期：2014-09-01 00:00:00
abstract：:Coronary blood flow is tightly coupled to myocardial oxygen consumption to maintain a consistently high level of myocardial oxygen extraction. This tight coupling has been proposed to depend on periarteriolar oxygen tension, signals released from cardiomyocytes (adenosine acting on K+(ATP) channels), and/or the endoth...
journal_title：Cell biochemistry and biophysics
pub_type： 杂志文章,评审
doi：10.1385/CBB:43:1:017
更新日期：2005-01-01 00:00:00
abstract：:Cell membranes consist of a fluidic medium of lipids and proteins that organize into specific submicron scale structures for signaling and molecular trafficking processes. These organized molecular assemblies form as a result of the structure and chemistry of the membrane components as well as the interactions of thos...
journal_title：Cell biochemistry and biophysics
pub_type： 杂志文章,评审
doi：10.1385/CBB:39:2:145
更新日期：2003-01-01 00:00:00
abstract：:Mechanics of cancer cells are directly linked to their metastatic potential, or ability to produce a secondary tumor at a distant site. Metastatic cells survive in the circulatory system in a non-adherent state, and can squeeze through barriers in the body. Such considerable structural changes in cells rely on rapid r...
journal_title：Cell biochemistry and biophysics
pub_type： 杂志文章
doi：10.1007/s12013-012-9356-z
更新日期：2012-07-01 00:00:00
abstract：:Leukocyte transendothelial migration is a multistep process coordinated by chemokine receptors, integrins and cell adhesion molecules. The interaction between leukocytes and endothelial cells is accompanied by bidirectional signaling in both cell types, which is initiated following formation of specialized, "docking" ...
journal_title：Cell biochemistry and biophysics
pub_type： 杂志文章,评审
doi：10.1385/cbb:38:3:305
更新日期：2003-01-01 00:00:00
abstract：:Calmodulin (CaM) binds to the FERM domain of 80 kDa erythrocyte protein 4.1R (R30) independently of Ca(2+) but, paradoxically, regulates R30 binding to transmembrane proteins in a Ca(2+)-dependent manner. We have previously mapped a Ca(2+)-independent CaM-binding site, pep11 (A(264)KKLWKVCVEHHTFFR), in 4.1R FERM domai...
journal_title：Cell biochemistry and biophysics
pub_type： 杂志文章
doi：10.1007/s12013-013-9758-6
更新日期：2014-05-01 00:00:00
abstract：:Curcumin is a yellow-orange dye widely used as a spice, food coloring and food preservative. It also exhibits a broad range of therapeutic effects against different disorders such as cancer, diabetes, or neurodegenerative diseases. As a compound insoluble in water curcumin accumulates in cell membranes and due to this...
journal_title：Cell biochemistry and biophysics
pub_type： 杂志文章
doi：10.1007/s12013-020-00906-5
更新日期：2020-06-01 00:00:00
abstract：:Molecular dynamics simulations, binding free energy calculations, principle component analysis (PCA), and residue interaction network analysis were employed in order to investigate the molecular mechanism of M184I single mutation which played pivotal role in making the HIV-1 reverse transcriptase (RT) totally resistan...
journal_title：Cell biochemistry and biophysics
pub_type： 杂志文章
doi：10.1007/s12013-015-0709-2
更新日期：2016-03-01 00:00:00
abstract：:cDNAs encoding two PDE-3 or cyclic GMP-inhibited (cGI) cyclic nucleotide phosphodiesterase (PDE) isoforms, RPDE-3B (RcGIP1) and HPDE-3A (HcGIP2), were cloned from rat (R) adipose tissue and human (H) heart cDNA libraries. Deletion and N- and C-terminal truncation mutants were expressed in Escherichia coli in order to ...
journal_title：Cell biochemistry and biophysics
pub_type： 杂志文章
doi：10.1007/BF02737830
更新日期：1998-01-01 00:00:00
abstract：:The increasing use of wireless communication devices has raised major concerns towards deleterious effects of microwave radiation on human health. The aim of the study was to demonstrate the effect of low-intensity microwave radiation on levels of monoamine neurotransmitters and gene expression of their key regulating...
journal_title：Cell biochemistry and biophysics
pub_type： 杂志文章
doi：10.1007/s12013-015-0576-x
更新日期：2015-09-01 00:00:00
abstract：:A fast-track clinical pathway is designed to streamline patient care delivery and maximize cost effectiveness. It has decreased postoperative length of stay (LOS) and hospital charges for many surgical procedures. However, data on clinical pathways after liver surgery are sparse. This study examined whether use of a f...
journal_title：Cell biochemistry and biophysics
pub_type： 杂志文章
doi：10.1007/s12013-011-9203-7
更新日期：2011-11-01 00:00:00
abstract：:Herpes simplex virus (HSV) is one of the most common viruses infecting humans and animals. Cellular adhesion is increased in HSV and plays a role in pathogenesis of inflammatory response during this viral infection. In our study, we studied a potential role of glycyrrhizin in disrupting cellular adhesion in HSV. We is...
journal_title：Cell biochemistry and biophysics
pub_type： 杂志文章
doi：10.1007/s12013-011-9271-8
更新日期：2012-01-01 00:00:00
abstract：:In recent studies, sulforaphane (SFN) has been seen to demonstrate antioxidant and anti-tumor activities as well as potent chemopreventive action against cancer. The present study investigates the anti-proliferation (using MTT assay, SFN demonstrated cytotoxic activity against GBM 8401 cell with IC(50) values at 35.52...
journal_title：Cell biochemistry and biophysics
pub_type： 杂志文章
doi：10.1007/s12013-012-9360-3
更新日期：2012-07-01 00:00:00
abstract：:Although aggressive therapeutic regimen has been applied in the treatment of Glioblastoma (GBM), the prognosis of patients with GBM remains poor. Preclinical studies have demonstrated the efficacy of Suntinib in GBM both in vitro and in vivo. In this study, we showed that the cytotoxicity was enhanced by transfection ...
journal_title：Cell biochemistry and biophysics
pub_type： 杂志文章
doi：10.1007/s12013-014-0501-8
更新日期：2015-06-01 00:00:00
abstract：:The aim of this study was to evaluate the effect of tumor necrosis factor-alpha (TNF-α) inhibitor-infliximab on ankylosing spondylitis (AS) patients and detect the serum level of HLA-B27 and PDCD-1 before and after TNF inhibitor treatment. 138 patients at active stage of AS were treated with infliximab; serum was coll...
journal_title：Cell biochemistry and biophysics
pub_type： 杂志文章
doi：10.1007/s12013-014-0082-6
更新日期：2014-11-01 00:00:00
abstract：:The CEP genes play a pivotal role in the replication of the cell. CEP family proteins form the major constituents of the centrosome and play a prominent role in centriole biogenesis and in cell replication. Alteration in CEP genes will result in disruption of cell cycle that may in turn cause cancer. In our study, we ...
journal_title：Cell biochemistry and biophysics
pub_type： 杂志文章
doi：10.1007/s12013-014-0153-8
更新日期：2014-12-01 00:00:00
abstract：:There are over 10,000 C2H2-type zinc finger (ZF) domains distributed among more than 1,000 ZF proteins in the human genome. These domains are frequently observed to be involved in sequence-specific DNA binding, and uncharacterized domains are typically assumed to facilitate DNA interactions. However, some ZFs also fac...
journal_title：Cell biochemistry and biophysics
pub_type： 杂志文章
doi：10.1007/s12013-008-9007-6
更新日期：2008-01-01 00:00:00
abstract：:The peritoneal mesothelium acts as a regulator of serosal responses to injury, infection, and neoplastic diseases. After inflammation of the serosal surfaces, proinflammatory cytokines induce an "activated" mesothelial cell phenotype, the mitochondrial aspect of which has not previously been studied. After incubation ...
journal_title：Cell biochemistry and biophysics
pub_type： 杂志文章
doi：10.1385/CBB:44:2:179
更新日期：2006-01-01 00:00:00
abstract：:Our objective was to explore the effects of miR-92a and miR-126 on myocardial apoptosis in mouse ischemia-reperfusion model and further investigate the underlying mechanisms. Eighteen Kunming mice were selected and randomly divided into sham operation group and ischemia-reperfusion group with nine mice in each group. ...
journal_title：Cell biochemistry and biophysics
pub_type： 杂志文章
doi：10.1007/s12013-014-0149-4
更新日期：2014-12-01 00:00:00
abstract：:The possibility that binuclear dinitrosyl iron complexes with glutathione and cysteine (DNIC-GSН and B-DNIC-Cys) have a strong cytotoxic effect on the growth of endometrioid tumours (EMT) in rats with surgically induced experimental endometriosis established in our previous studies has been supported with experimental...
journal_title：Cell biochemistry and biophysics
pub_type： 杂志文章
doi：10.1007/s12013-019-00865-6
更新日期：2019-03-01 00:00:00
abstract：:Hepatocyte growth factor (HGF) has been known to enhance the growth of normal hepatocytes, but also to inhibit the growth of neoplastic cells. This article examines the involvement of HGF in the hepatocarcinogenesis caused by peroxisome proliferators (PPs). Up to 78 wk after male F-344 rats were orally given (4-chloro...
journal_title：Cell biochemistry and biophysics
pub_type： 杂志文章
doi：10.1385/cbb:32:1-3:221
更新日期：2000-01-01 00:00:00
abstract：:The phosphatidylinositol 3-kinase/AKT (PI3K/AKT) pathway plays a critical role in human cancer. We determined the expression patterns of class I PI3K catalytic subunits and evaluated their importance in the development or progression of colorectal cancer (CRC). For this purpose, expression of class I PI3K isoforms was...
journal_title：Cell biochemistry and biophysics
pub_type： 杂志文章
doi：10.1007/s12013-011-9257-6
更新日期：2012-01-01 00:00:00
abstract：:Mutations of the retinoblastoma tumor suppressor, pRb, or its cyclin-cyclin-dependent kinase (CDK) regulatory kinases or CDK inhibitors, allows unrestrained E2F activity, leading to unregulated cell cycle progression. However, overexpression of E2F-1 also sensitizes cells to apoptosis, suggesting that targeting this p...
journal_title：Cell biochemistry and biophysics
pub_type： 杂志文章
doi：10.1385/CBB:33:2:199
更新日期：2000-01-01 00:00:00
abstract：:The elimination of viral covalently closed circular DNA (cccDNA) from the nucleus of infected hepatocytes is an obstacle to achieving sustained viral clearance during antiviral therapy of chronic hepatitis B virus (HBV) infection. The aim of our study was to determine whether treatment with siRNA is able to suppress v...
journal_title：Cell biochemistry and biophysics
pub_type： 杂志文章
doi：10.1007/s12013-014-9847-1
更新日期：2014-07-01 00:00:00
abstract：:The overview demonstrates how the use of only one physico-chemical approach, viz., the electron paramagnetic resonance method, allowed detection and identification of dinitrosyl iron complexes with thiol-containing ligands in various animal and bacterial cells. These complexes are formed in biological objects in the p...
journal_title：Cell biochemistry and biophysics
pub_type： 杂志文章,评审
doi：10.1007/s12013-017-0811-8
更新日期：2018-06-01 00:00:00
abstract：:The purpose of this study is to investigate the analgesic effect of intrathecal injection of chemokine receptor CCR2 antagonist RS102895, and its effect on spinal expression of N-methyl-D-aspartate (NMDA) receptor NR2B subunit, neuronal nitric oxide synthase (nNOS), and SIGIRR in a rat model of bone cancer pain (BCP)....
journal_title：Cell biochemistry and biophysics
pub_type： 杂志文章
doi：10.1007/s12013-014-0510-7
更新日期：2015-06-01 00:00:00
abstract：:Far from now are the days when investigators raced to identify the proteolytic system responsible for the degradation of their favorite protein. Nowadays, it is well accepted that a given protein can be degraded by different systems depending on factors such as cell type, cellular conditions, or functionality of each ...
journal_title：Cell biochemistry and biophysics
pub_type： 杂志文章,评审
doi：10.1007/s12013-013-9623-7
更新日期：2013-09-01 00:00:00
abstract：:The objective of this study was to analyze the enhancement patterns at dynamic gadolinium-enhanced T1-weighted MR imaging in patients of Hepatocellular Carcinoma (HCC) with associated Budd-Chiari syndrome (BCS). The MR imaging findings in 10 patients of HCC with associated BCS were compared to those of 32 other patien...
journal_title：Cell biochemistry and biophysics
pub_type： 杂志文章
doi：10.1007/s12013-014-9970-z
更新日期：2014-09-01 00:00:00
