Abstract：
:There are over 10,000 C2H2-type zinc finger (ZF) domains distributed among more than 1,000 ZF proteins in the human genome. These domains are frequently observed to be involved in sequence-specific DNA binding, and uncharacterized domains are typically assumed to facilitate DNA interactions. However, some ZFs also facilitate binding to proteins or RNA. Over 100 Cys2-His2 (C2H2) ZF-protein interactions have been described. We initially attempted a bioinformatics analysis to identify sequence features that would predict a DNA- or protein-binding function. These efforts were complicated by several issues, including uncertainties about the full functional capabilities of the ZFs. We therefore applied an unbiased approach to directly examine the potential for ZFs to facilitate DNA or protein interactions. The human OLF-1/EBF associated zinc finger (OAZ) protein was used as a model. The human O/E-1-associated zinc finger protein (hOAZ) contains 30 ZFs in 6 clusters, some of which have been previously indicated in DNA or protein interactions. DNA binding was assessed using a target site selection (CAST) assay, and protein binding was assessed using a yeast two-hybrid assay. We observed that clusters known to bind DNA could facilitate specific protein interactions, but clusters known to bind protein did not facilitate specific DNA interactions. Our primary conclusion is that DNA binding is a more restricted function of ZFs, and that their potential for mediating protein interactions is likely greater. These results suggest that the role of C2H2 ZF domains in protein interactions has probably been underestimated. The implication of these findings for the prediction of ZF function is discussed.
journal_name
Cell Biochem Biophysjournal_title
Cell biochemistry and biophysicsauthors
Brayer KJ,Kulshreshtha S,Segal DJdoi
10.1007/s12013-008-9007-6subject
Has Abstractpub_date
2008-01-01 00:00:00pages
9-19issue
1eissn
1085-9195issn
1559-0283journal_volume
51pub_type
杂志文章abstract：:Given that vascular endothelial cells play an important role in the modulation of vascular structure and function, we hypothesized that endocardial endothelial cells (EECs) may have a modulator role in regulating the cardiac interstitial cells. Endocardial endothelial cells were isolated from freshly collected pig hea...
journal_title：Cell biochemistry and biophysics
pub_type： 杂志文章
doi：10.1385/cbb:47:1:65
更新日期：2007-01-01 00:00:00
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journal_title：Cell biochemistry and biophysics
pub_type： 临床试验,杂志文章,多中心研究
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journal_title：Cell biochemistry and biophysics
pub_type： 杂志文章
doi：10.1007/s12013-014-0135-x
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pub_type： 杂志文章,评审
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journal_title：Cell biochemistry and biophysics
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更新日期：2012-04-01 00:00:00
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journal_title：Cell biochemistry and biophysics
pub_type： 杂志文章
doi：10.1007/s12013-014-0283-z
更新日期：2015-03-01 00:00:00
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journal_title：Cell biochemistry and biophysics
pub_type： 杂志文章,收录出版
doi：10.1007/s12013-014-0053-y
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journal_title：Cell biochemistry and biophysics
pub_type： 杂志文章
doi：10.1007/s12013-013-9640-6
更新日期：2013-01-01 00:00:00
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journal_title：Cell biochemistry and biophysics
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journal_title：Cell biochemistry and biophysics
pub_type： 杂志文章,评审
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journal_title：Cell biochemistry and biophysics
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更新日期：2013-05-01 00:00:00
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journal_title：Cell biochemistry and biophysics
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journal_title：Cell biochemistry and biophysics
pub_type： 杂志文章,随机对照试验
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更新日期：2015-05-01 00:00:00
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journal_title：Cell biochemistry and biophysics
pub_type： 杂志文章
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更新日期：2013-09-01 00:00:00
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pub_type： 杂志文章
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更新日期：2015-12-01 00:00:00
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journal_title：Cell biochemistry and biophysics
pub_type： 杂志文章,评审
doi：10.1007/s12013-007-0030-9
更新日期：2007-01-01 00:00:00
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journal_title：Cell biochemistry and biophysics
pub_type： 杂志文章
doi：10.1007/s12013-012-9355-0
更新日期：2012-07-01 00:00:00
