Abstract：
:It has been long known that the oncogenic extracellular environment plays an indispensable role in developing and nurturing cancer cell progression and in resistance to standard treatments. However, by how much the biophysical components of tumour extracellular environment contribute to these processes is uncertain. In particular, the topographic environment is scarcely explored. The biophysical modulation of cell behaviour is primarily facilitated through mechanotransduction-associated mechanisms, including focal adhesion and Rho/ROCK signalling. Dysregulation of these pathways is commonly observed in ovarian cancer and, therefore, biophysical modulation of these mechanisms may be of great importance to ovarian cancer development and progression. In this work, aspects of the biophysical environment were explored using a bioimprinting technique. The study showed that topography-mediated substrate sensing delayed cell attachment, however, cell-cell interactions overrode the effect of topography in some cell lines, such as OVCAR-5. Also, 3D topographical cues were shown to modulate the inhibition of focal adhesion and Rho signalling, which resulted in higher MAPK activity in cells on the bioimprints. It was revealed that c-Src is vital to the biophysical modulation of cell proliferation and inhibition of c-Src could downregulate biophysically modulated MAPK activity. This study provides evidence that the biophysical extracellular environment affects key intracellular mechanisms associated with tumourigenicity in ovarian cancer cells.
journal_name
Cell Biochem Biophysjournal_title
Cell biochemistry and biophysicsauthors
Sarwar M,Sykes PH,Chitcholtan K,Evans JJdoi
10.1007/s12013-020-00964-9subject
Has Abstractpub_date
2021-01-12 00:00:00eissn
1085-9195issn
1559-0283pii
10.1007/s12013-020-00964-9pub_type
杂志文章abstract：:The American Diabetes Association announces funding for clinical and basic science studies in three major areas of research that focus on cell-based insulin replacement in type 1 diabetes: 1. Genetic engineering of nonpancreatic cells into glucose-sensitive insulin-producing cells; 2. Transforming stem cells, pancreat...
journal_title：Cell biochemistry and biophysics
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更新日期：2004-01-01 00:00:00
abstract：:Fibroblasts can be reprogrammed by ectopic expression of reprogramming factors to yield induced pluripotent stem (iPS) cells that are capable of transdifferentiating into diverse types of somatic cell lines. In this study, we examined if functional cardiomyocytes (CMs) can be produced from mouse cardiac fibroblasts (C...
journal_title：Cell biochemistry and biophysics
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abstract：:Mutations of the retinoblastoma tumor suppressor, pRb, or its cyclin-cyclin-dependent kinase (CDK) regulatory kinases or CDK inhibitors, allows unrestrained E2F activity, leading to unregulated cell cycle progression. However, overexpression of E2F-1 also sensitizes cells to apoptosis, suggesting that targeting this p...
journal_title：Cell biochemistry and biophysics
pub_type： 杂志文章
doi：10.1385/CBB:33:2:199
更新日期：2000-01-01 00:00:00
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pub_type： 杂志文章
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journal_title：Cell biochemistry and biophysics
pub_type： 杂志文章,评审
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pub_type： 杂志文章
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journal_title：Cell biochemistry and biophysics
pub_type： 杂志文章,评审
doi：10.1007/BF02737882
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journal_title：Cell biochemistry and biophysics
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journal_title：Cell biochemistry and biophysics
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pub_type： 杂志文章
doi：10.1007/s12013-014-0283-z
更新日期：2015-03-01 00:00:00
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journal_title：Cell biochemistry and biophysics
pub_type： 杂志文章,评审
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更新日期：2012-01-01 00:00:00
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journal_title：Cell biochemistry and biophysics
pub_type： 杂志文章,多中心研究
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更新日期：2015-11-01 00:00:00
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journal_title：Cell biochemistry and biophysics
pub_type： 杂志文章
doi：10.1007/s12013-009-9071-6
更新日期：2010-04-01 00:00:00
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journal_title：Cell biochemistry and biophysics
pub_type： 杂志文章
doi：10.1007/s12013-014-0184-1
更新日期：2015-01-01 00:00:00
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journal_title：Cell biochemistry and biophysics
pub_type： 杂志文章,评审
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更新日期：2014-07-01 00:00:00
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journal_title：Cell biochemistry and biophysics
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abstract：:The transforming growth factor (TGF) superfamily encompasses about 30 members in mammals. The effect of TGF-beta subfamily members is exerted and regulated via selective pathways of synthesis and signaling that involve activation of latent TGF-beta, specific and high-affinity binding to cell membrane serine/threonine ...
journal_title：Cell biochemistry and biophysics
pub_type： 杂志文章,评审
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更新日期：2005-01-01 00:00:00
abstract：:Our objective is to investigate the promoting effect of hypoxic preconditioning combined with microbubble (MB)-mediated ultrasound (US) on the SDF-1/CXCR4 expression and the migration ability of mesenchymal stem cells (MSCs). Based on the uniform design, the parameters of MB-mediated US, such as the total treatment ti...
journal_title：Cell biochemistry and biophysics
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更新日期：2015-12-01 00:00:00
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journal_title：Cell biochemistry and biophysics
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更新日期：2012-07-01 00:00:00
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journal_title：Cell biochemistry and biophysics
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更新日期：2006-01-01 00:00:00
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journal_title：Cell biochemistry and biophysics
pub_type： 杂志文章
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更新日期：2015-09-01 00:00:00
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journal_title：Cell biochemistry and biophysics
pub_type： 杂志文章
doi：10.1007/s12013-014-9970-z
更新日期：2014-09-01 00:00:00
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journal_title：Cell biochemistry and biophysics
pub_type： 杂志文章
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更新日期：2013-05-01 00:00:00
abstract：:In previous work, decolorization of malachite green (MG) was studied in Aspergillus niger in the presence and absence of calcium chloride stress. Decolorization took place within 24 h, and a signal transduction process that initiated MG decolorization was suggested to be involved. In the present study, further investi...
journal_title：Cell biochemistry and biophysics
pub_type： 杂志文章
doi：10.1007/s12013-013-9661-1
更新日期：2013-01-01 00:00:00
abstract：:H5N1 is a subtype of the influenza A virus that can cause disease in humans and many other animal species. Oseltamivir (Tamiflu) is a potent and selective antiviral drug employed to fight the flu virus in infected individuals by inhibiting neuraminidase (NA), a flu protein responsible for the release and spread of the...
journal_title：Cell biochemistry and biophysics
pub_type： 杂志文章
doi：10.1007/s12013-013-9709-2
更新日期：2014-03-01 00:00:00
abstract：:Integrative medicine therapy using traditional Chinese medicine (TCM) combined with western medicine has shown some advantages in treating heart failure (HF), such as holistic concept; multi-target treatment; dialectical logic; personalized therapy; formulae compatibility; and reduction of side effects of western medi...
journal_title：Cell biochemistry and biophysics
pub_type： 杂志文章,评审
doi：10.1007/s12013-014-0466-7
更新日期：2015-06-01 00:00:00
abstract：:Discovered roughly 10 yr ago, Jak2 tyrosine kinase has emerged as a critical molecule in mammalian development, physiology, and disease. Here, we review the early history of Jak2 and its role in health and disease. We will also review its critical role in mediating cytokine-dependent signal transduction. Additionally,...
journal_title：Cell biochemistry and biophysics
pub_type： 杂志文章,评审
doi：10.1385/cbb:41:2:207
更新日期：2004-01-01 00:00:00
abstract：:This study is aimed to investigate the efficacy and underlying the mechanism of propofol in treatment of ischemia reperfusion (IR)-induced lung injury in rats, providing a novel insight of therapeutic strategy for IR-induced lung injury. 120 healthy SD rats were selected and randomly divided into sham operation group,...
journal_title：Cell biochemistry and biophysics
pub_type： 杂志文章
doi：10.1007/s12013-014-0088-0
更新日期：2014-12-01 00:00:00
