Effect of bone morphogenetic protein 4 in the human brain glioma cell line U251.

Abstract:

:The role of bone morphogenetic protein 4 (BMP4) in gliomas is not clear. We hypothesized that BMP4 inhibits proliferation in the brain glioma cell line U251 through a signaling pathway involving BMP4 and the mothers against decapentaplegic homolog 4 (SMAD4) protein. We exposed U251 cells to Adriamycin (1 g) for 48 h; cell proliferation (MTT assay), expression of BMP4 and SMAD4 (mRNA: qPCR; protein: Western blot) were studied. We further altered expression of BMP4 by overexpression or siRNA silencing, and documented cell responses to Adriamycin. Proliferation of U251 cells was significantly inhibited upon exposure to Adriamycin. This inhibition was associated with increased expression of BMP4. Further, proliferation of U251 cells was inhibited when BMP4 was overexpressed. BMP4 expression negatively correlated with expression of SMAD4, such that elevated levels of BMP4 were associated with decreased expression of SMAD4 and vice versa. The Adriamycin-induced inhibition of proliferation of U251 cells was attenuated when BMP4 was knocked down by siRNA. To conclude, BMP4 is associated with inhibition of proliferation of U251 cells; the effects of BMP4 involve the BMP4-Smad signaling pathway. BMP4 has a potential as a target for glioma therapy.

journal_name

Cell Biochem Biophys

authors

Liu B,Tian D,Yi W,Wu L,Cai Q,Dong H,Shen H,Ji B,Wang L,Zhang S,Ruan D,Chen Q

doi

10.1007/s12013-010-9095-y

subject

Has Abstract

pub_date

2010-11-01 00:00:00

pages

91-6

issue

2

eissn

1085-9195

issn

1559-0283

journal_volume

58

pub_type

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