Abstract:
:A method is presented to separate rabbit cardiac ventricular myosin isoenzymes (V(1), V(2), V(3)), which are large and important contractile proteins. This polyacrylamide gel electrophoresis--using a slab minigel format--does not involve preparation of an acrylamide gradient or denaturing conditions. The isoenzyme migration order was confirmed through identification with an anti beta-myosin heavy chain in cardiac ventricles (i.e., V(3)) antibody. Extracts from atrial and soleus muscle were used as positive control for V(1) and V(3), respectively. The relative quantification was obtained densitometrically and analyzed via TINA/Software. The reproducibility of method was additionally tested. The procedure employs Coomassie blue staining and is rapid and reproducible. Thus, the method permits easy and economic analysis of myosin isoenzymes under native conditions.
journal_name
Cell Biochem Biophysjournal_title
Cell biochemistry and biophysicsauthors
Garcia Pomblum SC,Pomblum VJ,Gams E,Reiser PJ,Schipke JDdoi
10.1385/CBB:38:1:33subject
Has Abstractpub_date
2003-01-01 00:00:00pages
33-40issue
1eissn
1085-9195issn
1559-0283pii
CBB:38:1:33journal_volume
38pub_type
杂志文章abstract::The coherent electromagnetic radiation (EMR) of the frequency of 51.8 and 53 GHz with low intensity (the power flux density of 0.06 mW/cm(2)) affected the growth of Escherichia coli K12(lambda) under fermentation conditions: the lowering of the growth specific rate was considerably (approximately 2-fold) increased wit...
journal_title:Cell biochemistry and biophysics
pub_type: 杂志文章
doi:10.1007/s12013-008-9020-9
更新日期:2008-01-01 00:00:00
abstract::A new intraoperative cisplatin administration method for patients with locally advanced gastric cancer (AGC) and without peritoneal metastasis, fibrin-sealant-delivered cisplatin chemotherapy, was reported, and its safety, pharmacokinetics, and efficacy were compared with cisplatin hyperthermic intraperitoneal perfusi...
journal_title:Cell biochemistry and biophysics
pub_type: 杂志文章,随机对照试验
doi:10.1007/s12013-014-0326-5
更新日期:2015-03-01 00:00:00
abstract::The present study aimed at detecting DNA damage and fragmentation as well as histone acetylation depending on oxidative stress caused by CCl4 intoxication. Also, the protective role of N-acetyl cysteine, a precursor for GSH, in DNA damage is investigated. Sixty rats were used in this study. In order to induce liver to...
journal_title:Cell biochemistry and biophysics
pub_type: 杂志文章
doi:10.1007/s12013-014-0031-4
更新日期:2014-11-01 00:00:00
abstract::Epidemiologic and experimental studies suggest that dietary fatty acids influence the development and progression of breast cancer. However, no clear data are present in literature that could demonstrate how n - 3 PUFA can interfere with breast cancer growth. It is suggested that these fatty acids might change the str...
journal_title:Cell biochemistry and biophysics
pub_type: 杂志文章
doi:10.1007/s12013-012-9365-y
更新日期:2012-09-01 00:00:00
abstract::Establishment of a detection platform for glioblastoma-dendritic cell (DC) vaccine preparation and to determine the efficacy of the vaccine in a clinical trial. Autologous glioblastoma-DC vaccine was prepared from a glioblast specimen procured from surgical resection. The specimen was used to enrich the vaccine with p...
journal_title:Cell biochemistry and biophysics
pub_type: 杂志文章
doi:10.1007/s12013-011-9265-6
更新日期:2012-01-01 00:00:00
abstract::Elevated blood pressure is regarded as an independent risk factor for cardiovascular diseases and diabetes. We examined the relation between hematocrit and pre-hypertension as well as the effect of sex, obesity, fasting glucose, and lipids in Chinese adults. The study samples were from a community-based health examina...
journal_title:Cell biochemistry and biophysics
pub_type: 杂志文章
doi:10.1007/s12013-014-0318-5
更新日期:2015-03-01 00:00:00
abstract::We have examined the association of insulin receptors (IR) and downstream signaling molecules with membrane microdomains in rat basophilic leukemia (RBL-2H3) cells following treatment with insulin or tris(2-pyridinecarbxylato)chromium(III) (Cr(pic)(3)). Single-particle tracking demonstrated that individual IR on these...
journal_title:Cell biochemistry and biophysics
pub_type: 杂志文章
doi:10.1007/s12013-011-9326-x
更新日期:2012-04-01 00:00:00
abstract::To explore therapeutic effects and underlying mechanism of Salubrinal combined with Ulinastatin (UTI) on acute Paraquat (PQ) poisoning. Four hundred rats were randomly allocated into UTI group, SAL group, SAL + UTI and control group according to random number table with 100 rats in each group. Acute PQ poisoning model...
journal_title:Cell biochemistry and biophysics
pub_type: 杂志文章
doi:10.1007/s12013-014-0095-1
更新日期:2014-12-01 00:00:00
abstract::The prevalence of cardiovascular disease (CVD) is increasing dramatically especially in developing countries like India. CVD is a leading cause of morbidity and mortality. There has been a growing awareness of the role of nutrients in the prevention of CVD. One specific recommendation in the battle against CVD is the ...
journal_title:Cell biochemistry and biophysics
pub_type: 杂志文章,评审
doi:10.1007/s12013-014-0407-5
更新日期:2015-05-01 00:00:00
abstract::We identified significantly higher expression of the genes glycogen debranching enzyme 6 (AGL), enolase 1 (ENOSF1), ectonucleotide pyrophosphatase 2 (ENPP2_1), glutathione S-transferase 3 (GSTM3_3) and mannosidase (MAN2B2) from human left cerebrums versus chimpanzees. Yet the distinct low- and high-expression AGL, ENO...
journal_title:Cell biochemistry and biophysics
pub_type: 杂志文章
doi:10.1007/s12013-011-9232-2
更新日期:2011-12-01 00:00:00
abstract::We wished to study the efficacy and safety of the retrograde ligation of short hepatic veins (SHVs) and the right hepatic vein (HV) through the retrohepatic tunnel in patients who underwent hemihepatectomy due to large hepatic carcinoma in the right lobe of the liver. Right hemihepatectomy was performed in 23 patients...
journal_title:Cell biochemistry and biophysics
pub_type: 临床试验,杂志文章
doi:10.1007/s12013-011-9268-3
更新日期:2012-01-01 00:00:00
abstract::The aim of this study is to examine the efficacy of alprostadil liposomal preparation in the treatment of blue toe syndrome. As many as 32 patients with blue toe syndrome were randomized into the test group and a control group. Patients out of the test group were treated with alprostadil liposomal preparation, while t...
journal_title:Cell biochemistry and biophysics
pub_type: 杂志文章,随机对照试验
doi:10.1007/s12013-014-0448-9
更新日期:2015-05-01 00:00:00
abstract::3-Iodothyronamine (T1AM) is regarded as a hormone-like substance thanks to its endogenous nature, its interaction with specific receptors trace amine-associated receptor 1 and its biological effects. We characterized T1AM transport and conversion in an in vitro culture of H9c2 murine cells, after a T1AM bolus injectio...
journal_title:Cell biochemistry and biophysics
pub_type: 杂志文章
doi:10.1007/s12013-013-9680-y
更新日期:2014-01-01 00:00:00
abstract::By using a functional approach of reconstituting detergent-solubilized membrane proteins into liposomes and following their function in patch-clamp experiments, we identified a novel mechanosensitive (MS) channel in the thermophilic cell wall-less archaeon Thermoplasma volcanium. Sodium dodecyl sulfate polyacrylamide ...
journal_title:Cell biochemistry and biophysics
pub_type: 杂志文章
doi:10.1385/CBB:34:3:321
更新日期:2001-01-01 00:00:00
abstract::Neural stem cells (NSCs) have widely been used in the treatment of human neurological disorders as cell therapy via intracerebral or intraventricular infusion. However, the migration mechanism required for NSCs homing and recruitment remains to be elucidated. Recently, SDF-1/CXCR4 axis was shown to be responsible for ...
journal_title:Cell biochemistry and biophysics
pub_type: 杂志文章
doi:10.1007/s12013-014-0103-5
更新日期:2014-12-01 00:00:00
abstract::Leptin is a protein hormone which plays a critical role in the regulation of both body-weight through reducing food intake and stimulating energy expenditure. Several polymorphisms in leptin gene (LEP), which encodes for leptin, have been described. However, its association with obesity is still controversial. Therefo...
journal_title:Cell biochemistry and biophysics
pub_type: 杂志文章
doi:10.1007/s12013-012-9427-1
更新日期:2013-03-01 00:00:00
abstract::A number of case-control studies have been conducted to investigate the relationship between the ATP-binding cassette transporter A1 (ABCA1) gene polymorphisms and risk of coronary heart disease (CHD). However, the results have been inconclusive. The purpose of the present study is to investigate whether this polymorp...
journal_title:Cell biochemistry and biophysics
pub_type: 杂志文章,meta分析
doi:10.1007/s12013-014-0161-8
更新日期:2015-01-01 00:00:00
abstract::The number of fatalities and economic losses caused by the Ebola virus infection across the planet culminated in the havoc that occurred between August and November 2014. However, little is known about the molecular protein profile of this devastating virus. This work represents a thorough bioinformatics analysis of t...
journal_title:Cell biochemistry and biophysics
pub_type: 杂志文章
doi:10.1007/s12013-018-0839-4
更新日期:2018-09-01 00:00:00
abstract::Bortezomib is effective in the therapy of multiple myeloma (MM), but causes infections that are different from those associated with conventional chemotherapy. It is important to identify the risk factors that facilitate infections associated with bortezomib therapy. In the present report, we sought to (1) define the ...
journal_title:Cell biochemistry and biophysics
pub_type: 临床试验,杂志文章
doi:10.1007/s12013-014-0224-x
更新日期:2015-01-01 00:00:00
abstract::A rapid and sensitive liquid chromatography-tandem mass spectrometry assay (LC-MS/MS) with electrospray ionization was developed and validated for the quantitative determination of the concentration of methotrexate (MTX) enantiomers in intracellular and extracellular fluids of HepG2 cells. The analytes were extracted ...
journal_title:Cell biochemistry and biophysics
pub_type: 杂志文章
doi:10.1007/s12013-013-9666-9
更新日期:2013-01-01 00:00:00
abstract::A Caulimovirus-based hybrid-promoter, EFCFS, was derived by fusing the distal region (-227 to -54, FUAS) of Figwort mosaic virus full-length transcript promoter (F20) with the core promoter (-151 to +12, FS3CP) domain of Figwort mosaic virus sub-genomic transcript promoter (FS3). The hybrid-promoter (EFCFS) showed enh...
journal_title:Cell biochemistry and biophysics
pub_type: 杂志文章
doi:10.1007/s12013-012-9359-9
更新日期:2012-07-01 00:00:00
abstract::Azurin, a small blue copper protein from the bacterial species Pseudomonas aeruginosa, is mostly a beta-sheet protein arranged into a single domain. Previous folding studies have shown that the equilibrium denaturation of the holoprotein follows a two-state process; however, upon removal of the copper, the denaturatio...
journal_title:Cell biochemistry and biophysics
pub_type: 杂志文章
doi:10.1385/cbb:36:1:19
更新日期:2002-01-01 00:00:00
abstract::Recent studies designed to assess the relationship between aortic compliance and heterogeneity of heart electrical activity has shown that hypertrophy aggravates repolarization disturbances in the myocardium. Numerous mechanisms of electrical instability and inhomogeneity associated with left ventricular hypertrophy a...
journal_title:Cell biochemistry and biophysics
pub_type: 杂志文章,评审
doi:10.1007/s12013-014-9850-6
更新日期:2014-07-01 00:00:00
abstract::Cellular responses to external signals often reflect alterations in gene expression. The activation of cell surface hormone or growth factor receptors upon the binding of appropriate ligands mobilizes signal transduction cascades that can ultimately impact the activity of defined sets of transcription factors. The int...
journal_title:Cell biochemistry and biophysics
pub_type: 杂志文章,评审
doi:10.1007/BF02737882
更新日期:1999-01-01 00:00:00
abstract::To examine the antiproliferative effect of the combination of docetaxel and sorafenib, applied to the representative non-small cell lung cancer cell line A549 cells either wild type or with acquired resistance to docetaxel (A549/D). The aim of this study is to evaluate the synergistic effect of combination treatment o...
journal_title:Cell biochemistry and biophysics
pub_type: 杂志文章
doi:10.1007/s12013-013-9722-5
更新日期:2014-03-01 00:00:00
abstract::Proper administration of antithrombotic and antiplatelet drugs after percutaneous coronary intervention (PCI) in patients with acute coronary syndrome (ACS) and renal insufficiency is a challenging task. In this study, we utilized Fondaparinux and Tirofiban (either separately or combined) to treat post-PCI patients wi...
journal_title:Cell biochemistry and biophysics
pub_type: 杂志文章,随机对照试验
doi:10.1007/s12013-015-0580-1
更新日期:2015-12-01 00:00:00
abstract::Insulin, the major secreted product of the beta-cells of the islets of Langerhans, is initially synthesized as a precursor (preproinsulin), from which the mature hormone is excised by a series of proteolytic cleavages. This review provides a personal narrative of some of the key research projects leading to the identi...
journal_title:Cell biochemistry and biophysics
pub_type: 杂志文章,评审
doi:10.1385/cbb:40:3:143
更新日期:2004-01-01 00:00:00
abstract::Gaining large scale success was quite common in the later period of industrialization for hospitals in China. Today, Chinese hospital management face such problems as service inefficiency, high human resources cost, and low rate of capital use. This study analyzes the refined management chain of the Wuxi No. 2 People'...
journal_title:Cell biochemistry and biophysics
pub_type: 杂志文章
doi:10.1007/s12013-014-0395-5
更新日期:2015-05-01 00:00:00
abstract::We apply methods from statistical physics (histograms, correlation functions, fractal dimensions, and singularity spectra) to characterize large-scale structure of the distribution of nucleotides along genomic sequences. We discuss the role of the extension of noncoding segments ("junk DNA") for the genomic organizati...
journal_title:Cell biochemistry and biophysics
pub_type: 杂志文章
doi:10.1385/CBB:42:2:145
更新日期:2005-01-01 00:00:00
abstract::The purpose of this study was to investigate the incidence and risk factors of infections associated with totally implantable venous-access ports (TIVAPs) in our hospital. From April 2007 through November 2013, a total of 1,026 patients with TIVAP were included in this study. We retrospectively analyzed the incidence ...
journal_title:Cell biochemistry and biophysics
pub_type: 杂志文章
doi:10.1007/s12013-014-0443-1
更新日期:2015-05-01 00:00:00