Electrophoretic separation of ventricular myosin isoenzymes using a native polyacrylamide minigel system.

Abstract:

:A method is presented to separate rabbit cardiac ventricular myosin isoenzymes (V(1), V(2), V(3)), which are large and important contractile proteins. This polyacrylamide gel electrophoresis--using a slab minigel format--does not involve preparation of an acrylamide gradient or denaturing conditions. The isoenzyme migration order was confirmed through identification with an anti beta-myosin heavy chain in cardiac ventricles (i.e., V(3)) antibody. Extracts from atrial and soleus muscle were used as positive control for V(1) and V(3), respectively. The relative quantification was obtained densitometrically and analyzed via TINA/Software. The reproducibility of method was additionally tested. The procedure employs Coomassie blue staining and is rapid and reproducible. Thus, the method permits easy and economic analysis of myosin isoenzymes under native conditions.

journal_name

Cell Biochem Biophys

authors

Garcia Pomblum SC,Pomblum VJ,Gams E,Reiser PJ,Schipke JD

doi

10.1385/CBB:38:1:33

subject

Has Abstract

pub_date

2003-01-01 00:00:00

pages

33-40

issue

1

eissn

1085-9195

issn

1559-0283

pii

CBB:38:1:33

journal_volume

38

pub_type

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