当前位置： SCI文献检索 > CELL BIOCHEMISTRY AND BIOPHYSICS期刊下所有文献 > Insulin receptors and downstream substrates associate with membrane microdomains after treatment with insulin or chromium(III) picolinate.

Insulin receptors and downstream substrates associate with membrane microdomains after treatment with insulin or chromium(III) picolinate.

Abstract：

:We have examined the association of insulin receptors (IR) and downstream signaling molecules with membrane microdomains in rat basophilic leukemia (RBL-2H3) cells following treatment with insulin or tris(2-pyridinecarbxylato)chromium(III) (Cr(pic)(3)). Single-particle tracking demonstrated that individual IR on these cells exhibited reduced lateral diffusion and increased confinement within 100 nm-scale membrane compartments after treatment with either 200 nM insulin or 10 μM Cr(pic)(3). These treatments also increased the association of native IR, phosphorylated insulin receptor substrate 1 and phosphorylated AKT with detergent-resistant membrane microdomains of characteristically high buoyancy. Confocal fluorescence microscopic imaging of Di-4-ANEPPDHQ labeled RBL-2H3 cells also showed that plasma membrane lipid order decreased following treatment with Cr(pic)(3) but was not altered by insulin treatment. Fluorescence correlation spectroscopy demonstrated that Cr(pic)(3) did not affect IR cell-surface density or compete with insulin for available binding sites. Finally, Fourier transform infrared spectroscopy indicated that Cr(pic)(3) likely associates with the lipid interface in reverse-micelle model membranes. Taken together, these results suggest that activation of IR signaling in a cellular model system by both insulin and Cr(pic)(3) involves retention of IR in specialized nanometer-scale membrane microdomains but that the insulin-like effects of Cr(pic)(3) are due to changes in membrane lipid order rather than to direct interactions with IR.

journal_name

Cell Biochem Biophys

journal_title

Cell biochemistry and biophysics

authors

Al-Qatati A,Winter PW,Wolf-Ringwall AL,Chatterjee PB,Van Orden AK,Crans DC,Roess DA,Barisas BG

doi

10.1007/s12013-011-9326-x

subject

Has Abstract

pub_date

2012-04-01 00:00:00

pages

441-50

issue

3

eissn

1085-9195

issn

1559-0283

journal_volume

62

pub_type

杂志文章

相关文献

CELL BIOCHEMISTRY AND BIOPHYSICS文献大全
  • The Use of Calcium Channel Blockers in the Treatment of Coronary Spasm and Atrioventricular Block.

    abstract：:Calcium channel blockers have been used in the treatment of coronary artery spasm for many years. However, there is insufficient knowledge about their application to treat atrioventricular block caused by coronary spasm. Clinical data of five patients who were diagnosed with coronary spasm caused by atrioventricular b...

    journal_title：Cell biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1007/s12013-014-0498-z

    authors： Gao B,Zhang Z,Qian J,Cao C,Hua X,Chu M,He X,Zeng H

    更新日期：2015-06-01 00:00:00

  • Intrinsically semi-disordered state and its role in induced folding and protein aggregation.

    abstract：:Intrinsically disordered proteins (IDPs) refer to those proteins without fixed three-dimensional structures under physiological conditions. Although experiments suggest that the conformations of IDPs can vary from random coils, semi-compact globules, to compact globules with different contents of secondary structures,...

    journal_title：Cell biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1007/s12013-013-9638-0

    authors： Zhang T,Faraggi E,Li Z,Zhou Y

    更新日期：2013-01-01 00:00:00

  • Translating biomaterial properties to intracellular signaling.

    abstract：:Bioactive materials present important micro-environmental cues that induce specific intracellular signaling responses which ultimately determine cell behavior. For example, vascular endothelial cells on a normal vessel wall resist inflammation and thrombosis, but the same cells seeded on an artificial vascular graft o...

    journal_title：Cell biochemistry and biophysics

    pub_type： 杂志文章,评审

    doi：10.1007/s12013-009-9048-5

    authors： Caplan MR,Shah MM

    更新日期：2009-01-01 00:00:00

  • Expression of Treg subsets on intestinal T cell immunity and endotoxin translocation in porcine sepsis after severe burns.

    abstract：:In the present study, the changes of the regulatory T cells (Treg) expression, endotoxin translocation, and the relationships in intestinal lymph nodes were investigated in porcine sepsis induced by severe burns. Flow cytometry, western blot, and Tachypleus amebocyte lysate were applied to study after the burn injury ...

    journal_title：Cell biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1007/s12013-014-0116-0

    authors： Zu H,Li Q,Huang P

    更新日期：2014-12-01 00:00:00

  • Biorheological action of Ascaris lumbricoides larvae on human erythrocytes.

    abstract：:Previous studies have shown that A. lumbricoides extracts capture sialic acid (SA) from human red blood cells (RBC). The aim of this work was to study hemorheological alterations in vitro caused by parasite larvae. The biorheological action of three larva concentrates of first and second larval stage on group O erythr...

    journal_title：Cell biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1007/s12013-012-9425-3

    authors： de León PP,Del Balzo G,Riquelme B

    更新日期：2013-03-01 00:00:00

  • CT diagnosis in the thecoma-fibroma group of the ovarian stromal tumors.

    abstract：:The aim of this study was to investigate characteristic CT manifestations of the group of ovarian thecoma-fibroma. 24 patients (26 lesions) presenting with the ovarian thecoma-fibroma were analyzed retrospectively, and the diagnosis were confirmed by pathology after surgery. Our findings included: 22 patients were uni...

    journal_title：Cell biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1007/s12013-014-0288-7

    authors： Zhang Z,Wu Y,Gao J

    更新日期：2015-03-01 00:00:00

  • Advantages, Disadvantages, and Trend of Integrative Medicine in the Treatment of Heart Failure.

    abstract：:Integrative medicine therapy using traditional Chinese medicine (TCM) combined with western medicine has shown some advantages in treating heart failure (HF), such as holistic concept; multi-target treatment; dialectical logic; personalized therapy; formulae compatibility; and reduction of side effects of western medi...

    journal_title：Cell biochemistry and biophysics

    pub_type： 杂志文章,评审

    doi：10.1007/s12013-014-0466-7

    authors： Zhang P

    更新日期：2015-06-01 00:00:00

  • Unique structural changes in calcium-bound calmodulin upon interaction with protein 4.1R FERM domain: novel insights into the calcium-dependent regulation of 4.1R FERM domain binding to membrane proteins by calmodulin.

    abstract：:Calmodulin (CaM) binds to the FERM domain of 80 kDa erythrocyte protein 4.1R (R30) independently of Ca(2+) but, paradoxically, regulates R30 binding to transmembrane proteins in a Ca(2+)-dependent manner. We have previously mapped a Ca(2+)-independent CaM-binding site, pep11 (A(264)KKLWKVCVEHHTFFR), in 4.1R FERM domai...

    journal_title：Cell biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1007/s12013-013-9758-6

    authors： Nunomura W,Isozumi N,Nakamura S,Jinbo Y,Ohki S,Kidokoro S,Wakui H,Takakuwa Y

    更新日期：2014-05-01 00:00:00

  • Better prognosis in newborns with trisomy 13 who received intensive treatments: a retrospective study of 16 patients.

    abstract：:Intensive treatment for newborns with trisomy 13 is controversial because of their lethal prognosis. We report the better life prognosis of patients with trisomy 13 who received intensive treatment. At our hospital, we provided an intensive management to such patients including resuscitation and surgical procedures as...

    journal_title：Cell biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1007/s12013-012-9355-0

    authors： Tsukada K,Imataka G,Suzumura H,Arisaka O

    更新日期：2012-07-01 00:00:00

  • DNA microarray analysis for human congenital heart disease.

    abstract：:Right ventricular hypertrophy and failure are prominent features in cyanotic congenital heart disease, tetralogy of Fallot (TF). Patients with TF require primary cardiac surgery at a very young age. To gain insight into the underlying molecular mechanisms of right ventricular hypertrophy and to identify gene(s) involv...

    journal_title：Cell biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1385/CBB:44:1:001

    authors： Sharma HS,Peters TH,Moorhouse MJ,van der Spek PJ,Bogers AJ

    更新日期：2006-01-01 00:00:00

  • Coating and selective deposition of nanofilm on silicone rubber for cell adhesion and growth.

    abstract：:A recently developed method for surface modification, layer-by-layer (LbL) assembly, has been applied to silicone, and its ability to encourage endothelial cell growth and control cell growth patterns has been examined. The surfaces studied consisted of a precursor, with alternating cationic polyethyleneimine (PEI) an...

    journal_title：Cell biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1385/CBB:38:2:103

    authors： Ai H,Lvov YM,Mills DK,Jennings M,Alexander JS,Jones SA

    更新日期：2003-01-01 00:00:00

  • Multicenter study on adult growth hormone level in postoperative pituitary tumor patients.

    abstract：:The objective of this study is to observe the adult growth hormone level in postoperative pituitary tumor patients of multi-centers, and explore the change of hypophyseal hormones in postoperative pituitary tumor patients. Sixty patients with pituitary tumor admitted during March, 2011-March, 2012 were selected. Posto...

    journal_title：Cell biochemistry and biophysics

    pub_type： 临床试验,杂志文章,多中心研究

    doi：10.1007/s12013-014-0334-5

    authors： Cheng JM,Gu JW,Kuang YQ,Ma Y,Xia X,Yang T,Lu M,He WQ,Sun ZY,Zhang YC

    更新日期：2015-03-01 00:00:00

  • Novel c.2216T > C (p.I739T) mutation in exon 13 and c.1481T > A (p.L494X) mutation in exon 8 of MUT gene in a female with methylmalonic acidemia.

    abstract：:We report herein a 1.5-year-old girl with methylmalonic acidemia (MMA) in whom two missense mutations were found: a novel I739T mutation located in exon 13 and the L494X mutation in exon 8. The results of organic acid test showed a pronounced increase in methylmalonate excretion with increased methylcitrate and 3-OH-p...

    journal_title：Cell biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1007/s12013-013-9532-9

    authors： Imataka G,Sakamoto O,Yamanouchi H,Yoshihara S,Omura-Hasegawa Y,Tajima G,Arisaka O

    更新日期：2013-09-01 00:00:00

  • Expression and characterization of deletion recombinants of two cGMP-inhibited cyclic nucleotide phosphodiesterases (PDE-3).

    abstract：:cDNAs encoding two PDE-3 or cyclic GMP-inhibited (cGI) cyclic nucleotide phosphodiesterase (PDE) isoforms, RPDE-3B (RcGIP1) and HPDE-3A (HcGIP2), were cloned from rat (R) adipose tissue and human (H) heart cDNA libraries. Deletion and N- and C-terminal truncation mutants were expressed in Escherichia coli in order to ...

    journal_title：Cell biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1007/BF02737830

    authors： He R,Komas N,Ekholm D,Murata T,Taira M,Hockman S,Degerman E,Manganiello VC

    更新日期：1998-01-01 00:00:00

  • Toward mechanical manipulations of cell membranes and membrane proteins using an atomic force microscope: an invited review.

    abstract：:Recent advances in the use of the atomic force microscope (AFM) for manipulating cell membranes and membrane proteins are reviewed. Early pioneering work on measurements of the magnitude of the force required to create indentations with defined depth on their surfaces and to separate interacting pairs of avidin-biotin...

    journal_title：Cell biochemistry and biophysics

    pub_type： 杂志文章,评审

    doi：10.1385/CBB:39:3:257

    authors： Ikai A,Afrin R

    更新日期：2003-01-01 00:00:00

  • Mechanical induction of BMP-7 in osteocyte blocks glucocorticoid-induced apoptosis through PI3K/AKT/GSK3β pathway.

    abstract：:Mechanical loading is known to promote osteocyte survival, whereas glucocorticoid treatment results in osteocyte apoptosis. Here, we report that BMP-7, which was secreted by osteocyte in response to mechanical loading, exerts anti-apoptotic effect against dexamethasone-induced apoptosis of osteocytes. We further show ...

    journal_title：Cell biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1007/s12013-013-9543-6

    authors： Wang Z,Guo J

    更新日期：2013-11-01 00:00:00

  • Hydrogen sulfide endothelin-induced myocardial hypertrophy in rats and the mechanism involved.

    abstract：:The aim of the study was to evaluate the clinical efficacy of hydrogen sulfide (H2S) treatment on the endothelin-induced cardiac hypertrophy. Sixty-four adult male rats, weighing from 180 to 200 g, were randomly divided into four groups: ten in normal group, ten in sham group, 44 in model group established by inducing...

    journal_title：Cell biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1007/s12013-014-0113-3

    authors： Yang F,Liu Z,Wang Y,Li Z,Yu H,Wang Q

    更新日期：2014-12-01 00:00:00

  • Expression and purification of rotavirus proteins NSP5 and NSP6 in Escherichia coli.

    abstract：:Rotaviruses are one of the worldwide leading causes of gastroenteritis in children under 5 yr old. The rotavirus nonstructural NSP5 is a phosphoprotein implicated in viroplasms formation, whereas NSP6 could have a possible regulatory role of NSP5. It has been reported that N- and C-termini of NSP5 are important for th...

    journal_title：Cell biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1385/CBB:44:3:336

    authors： Samaniego-Hernández M,De León-Rodriguez A,Aparicio-Fabre R,Arias-Ortiz C,Barba de la Rosa AP

    更新日期：2006-01-01 00:00:00

  • Ecto-mesenchymal stem cells from facial process: potential for muscle regeneration.

    abstract：:Ecto-mesenchymal stem cells (EMSCs) originate from the cranial neural crest and participate in the formation of tooth, salivary, and muscle in early development stage. The transplantation of EMSCs, a potential source of myoblast stem cell, might improve muscle regeneration. The purpose of this study was to explore whe...

    journal_title：Cell biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1007/s12013-014-9964-x

    authors： Nie X,Xing Y,Deng M,Gang L,Liu R,Zhang Y,Wen X

    更新日期：2014-09-01 00:00:00

  • The Effect of Hydroxycamptothecin on Wound Healing Following Reduction of the Knee Intra-Articular Adhesion in Rabbits.

    abstract：:Topical application of 10-hydroxycamptothecin (HCPT) can reduce intra-articular scar adhesion after knee surgery, but the effect of HCPT on wound healing has not yet been elucidated. The study investigates the effect of the topical application of HCPT on wound healing after knee surgery in rabbits. Forty New Zealand w...

    journal_title：Cell biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1007/s12013-015-0593-9

    authors： Yan L,Sun Y,Li X,Liang Y,Zhu G,Wang J,Xiao T

    更新日期：2015-09-01 00:00:00

  • Construction and Application of a Refined Hospital Management Chain.

    abstract：:Gaining large scale success was quite common in the later period of industrialization for hospitals in China. Today, Chinese hospital management face such problems as service inefficiency, high human resources cost, and low rate of capital use. This study analyzes the refined management chain of the Wuxi No. 2 People'...

    journal_title：Cell biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1007/s12013-014-0395-5

    authors： Yi L,Hao A,Hu M,Huang P,Yuan H,Xing M

    更新日期：2015-05-01 00:00:00

  • Acteoside Presents Protective Effects on Cerebral Ischemia/reperfusion Injury Through Targeting CCL2, CXCL10, and ICAM1.

    abstract：:The objective of this study is to investigate the roles of acteoside (ACT) in cells with oxygen-glucose deprivation and reoxygenation (OGD/R)-induced injury and the underlying mechanisms. The differentially expressed genes (DEGs) in rats with middle cerebral artery occlusion were identified using GSE61616 data set. Ky...

    journal_title：Cell biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1007/s12013-020-00965-8

    authors： Wu W,Wu G,Cao D

    更新日期：2021-01-13 00:00:00

  • Effect of bone morphogenetic protein 4 in the human brain glioma cell line U251.

    abstract：:The role of bone morphogenetic protein 4 (BMP4) in gliomas is not clear. We hypothesized that BMP4 inhibits proliferation in the brain glioma cell line U251 through a signaling pathway involving BMP4 and the mothers against decapentaplegic homolog 4 (SMAD4) protein. We exposed U251 cells to Adriamycin (1 g) for 48 h; ...

    journal_title：Cell biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1007/s12013-010-9095-y

    authors： Liu B,Tian D,Yi W,Wu L,Cai Q,Dong H,Shen H,Ji B,Wang L,Zhang S,Ruan D,Chen Q

    更新日期：2010-11-01 00:00:00

  • Two-probe microfluorometry estimation of transmembrane potential (ΔΨ(p)) slight changes in individual hepatocytes under short-term hormone action.

    abstract：:Early events in individual hepatocytes of rat activated by adrenaline (10(-6)M) and phenylephrine (10(-5)M) have been investigated by quantitative image microfluorometry and microspectrofluorometry. Cationic DiOC2 and anionic SqSC4 probes have been used for image analysis and transmembrane potential (ΔΨ p) estimation ...

    journal_title：Cell biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1007/s12013-013-9569-9

    authors： Kavok NS,Malyukina MY,Borovoy IA,Obukchova EN,Klimov SA

    更新日期：2013-11-01 00:00:00

  • Influence of Val108/158Met COMT Gene Polymorphism on the Efficacy of Modified Electroconvulsive Therapy in Patients with Treatment Resistant Depression.

    abstract：:Depression is a common emotional disorder associated with increased risk of suicide and rate of disability. In this double-blinded control study, we tested the efficacy of modified electroconvulsive therapy (MECT) in patients with treatment resistant depression (TRD) using the Hamilton Depression Rating Scale for Depr...

    journal_title：Cell biochemistry and biophysics

    pub_type： 临床试验,杂志文章

    doi：10.1007/s12013-014-0361-2

    authors： Lin Z,He H,Zhang C,Wang Z,Jiang M,Li Q,Lan X,Zhang M,Huang X

    更新日期：2015-04-01 00:00:00

  • Implementation of a fast-track clinical pathway decreases postoperative length of stay and hospital charges for liver resection.

    abstract：:A fast-track clinical pathway is designed to streamline patient care delivery and maximize cost effectiveness. It has decreased postoperative length of stay (LOS) and hospital charges for many surgical procedures. However, data on clinical pathways after liver surgery are sparse. This study examined whether use of a f...

    journal_title：Cell biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1007/s12013-011-9203-7

    authors： Lin DX,Li X,Ye QW,Lin F,Li LL,Zhang QY

    更新日期：2011-11-01 00:00:00

  • Synergistic effect of phenformin in non-small cell lung cancer (NSCLC) ionizing radiation treatment.

    abstract：:Biguanides, used for anti-diabetic drugs, bring more attention in cancer research for their beneficial effects. Phenformin is more potent than metformin. However its potential application as a anti-cancer regent is far behind metformin. In order to investigate any beneficial effect of combination of Phenformin and rad...

    journal_title：Cell biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1007/s12013-014-0283-z

    authors： Wang J,Xia S,Zhu Z

    更新日期：2015-03-01 00:00:00

  • Estimation of Some Oxidative Stress Parameters and Blood Pressure After Administration of Endothelin-1 (ET-1) in Rats.

    abstract：:The aim of the study was to investigate changes in the plasma antioxidative activity and in lipid peroxidation after administration of endothelin-1 (ET-1) and endothelin receptor blockers and additionally, to estimate blood pressure. The study was performed on male Wistar rats (n = 6 per group) divided into 4 groups w...

    journal_title：Cell biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1007/s12013-014-0371-0

    authors： Kleniewska P,Kowalczyk A,Ciesla W,Goraca A

    更新日期：2015-04-01 00:00:00

  • A proteomic analysis of placental trophoblastic cells in preeclampsia-eclampsia.

    abstract：:To explore the proteomic changes of placental trophoblastic cells in preeclampsia-eclampsia (PE), placental trophoblastic cells from normally pregnant women and women with hypertension during gestational period were prepared by laser capture microdissection (LCM), and proteins isolated from these cells were subjected ...

    journal_title：Cell biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1007/s12013-013-9792-4

    authors： Ma K,Jin H,Hu R,Xiong Y,Zhou S,Ting P,Cheng Y,Yang Y,Yang P,Li X

    更新日期：2014-06-01 00:00:00

  • Protective effect of sRAGE on fetal development in pregnant rats with gestational diabetes mellitus.

    abstract：:To investigate the protective effect of secretory receptor for advanced glycation endproducts (sRAGE) on the fetal development using rat model of gestational diabetes mellitus (GDM). The model of pregnant rats with intrauterine hyperglycemia was established by intraperitoneal injection of 25 mg/kg streptozotocin (STZ)...

    journal_title：Cell biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1007/s12013-014-0233-9

    authors： Tang X,Qin Q,Xie X,He P

    更新日期：2015-03-01 00:00:00