Biorheological action of Ascaris lumbricoides larvae on human erythrocytes.

Abstract:

:Previous studies have shown that A. lumbricoides extracts capture sialic acid (SA) from human red blood cells (RBC). The aim of this work was to study hemorheological alterations in vitro caused by parasite larvae. The biorheological action of three larva concentrates of first and second larval stage on group O erythrocytes was analyzed by incubating the erythrocyte packed together with an equal volume of larvae (treated RBC) and PBS (control RBC). Distribution and parameters of aggregation (digital image analysis), aggregation kinetics (erythroaggregameter), and viscoelasticity (erythrodeformeter) were measured. The digital image analysis showed that all the larvae diminished the isolated cells percentage and increased the size of the formed aggregates. The aggregate formation velocity was lower in the treated than in the control. The deformability index (ID) values of treated RBC did not present variations with respect to those of the control, but a decrease in the erythrocyte elastic modulus (μ(m)) and membrane surface viscosity (η(m)) values was observed, indicating that the larvae not only induced a diminution in the membrane surface viscosity of RBC but also altered the dynamic viscoelasticity of the membrane. Experiments carried out in vitro support the conclusion that the contact between larvae and RBC produces hemorheological alterations.

journal_name

Cell Biochem Biophys

authors

de León PP,Del Balzo G,Riquelme B

doi

10.1007/s12013-012-9425-3

subject

Has Abstract

pub_date

2013-03-01 00:00:00

pages

237-42

issue

2

eissn

1085-9195

issn

1559-0283

journal_volume

65

pub_type

杂志文章
  • Structural adaptation in adult rabbit ventricular myocytes: influence of dynamic physical interaction with fibroblasts.

    abstract::The mechanism of induction of cardiomyocyte (CM) dedifferentiation, as seen in chronic hibernating myocardium, is largely unknown. Recently, a cellular model was proposed consisting of long-term cocultures of adult rabbit CMs and cardiac fibroblasts in which typical structural characteristics of hibernation-like dedif...

    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章

    doi:10.1385/CBB:44:1:119

    authors: Driesen RB,Verheyen FK,Dispersyn GD,Thoné F,Lenders MH,Ramaekers FC,Borgers M

    更新日期:2006-01-01 00:00:00

  • Interfacial Electrostatic Properties of Hydrated Mesoporous and Nanostructured Alumina Powders by Spin Labeling EPR.

    abstract::Acid-base equilibria and interfacial electrostatic properties of hydrated mesoporous and nanostructured alumina powders are determining factors for the use of these materials in heterogeneous catalysis and as a sorption media for filtration and chromatographic applications including life sciences. Here spin probe elec...

    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章

    doi:10.1007/s12013-016-0767-0

    authors: Kovaleva EG,Molochnikov LS,Stepanova DP,Pestov AV,Trofimov DG,Kirilyuk IA,Smirnov AI

    更新日期:2017-06-01 00:00:00

  • Insight into the oseltamivir resistance R292K mutation in H5N1 influenza virus: a molecular docking and molecular dynamics approach.

    abstract::H5N1 is a subtype of the influenza A virus that can cause disease in humans and many other animal species. Oseltamivir (Tamiflu) is a potent and selective antiviral drug employed to fight the flu virus in infected individuals by inhibiting neuraminidase (NA), a flu protein responsible for the release and spread of the...

    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章

    doi:10.1007/s12013-013-9709-2

    authors: Karthick V,Ramanathan K

    更新日期:2014-03-01 00:00:00

  • Studying single red blood cells under a tunable external force by combining passive microrheology with Raman spectroscopy.

    abstract::The dynamic micromechanical and structural properties of single human red blood cells are studied using a combination of dual trap optical tweezers and confocal Raman spectroscopy. Such a combination permits us to show a direct relationship between the rheological properties and chemical structure conformation. The fr...

    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章

    doi:10.1007/s12013-012-9439-x

    authors: Raj S,Wojdyla M,Petrov D

    更新日期:2013-04-01 00:00:00

  • Intrinsically semi-disordered state and its role in induced folding and protein aggregation.

    abstract::Intrinsically disordered proteins (IDPs) refer to those proteins without fixed three-dimensional structures under physiological conditions. Although experiments suggest that the conformations of IDPs can vary from random coils, semi-compact globules, to compact globules with different contents of secondary structures,...

    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章

    doi:10.1007/s12013-013-9638-0

    authors: Zhang T,Faraggi E,Li Z,Zhou Y

    更新日期:2013-01-01 00:00:00

  • The Use of Calcium Channel Blockers in the Treatment of Coronary Spasm and Atrioventricular Block.

    abstract::Calcium channel blockers have been used in the treatment of coronary artery spasm for many years. However, there is insufficient knowledge about their application to treat atrioventricular block caused by coronary spasm. Clinical data of five patients who were diagnosed with coronary spasm caused by atrioventricular b...

    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章

    doi:10.1007/s12013-014-0498-z

    authors: Gao B,Zhang Z,Qian J,Cao C,Hua X,Chu M,He X,Zeng H

    更新日期:2015-06-01 00:00:00

  • Inhibition of microtubule dynamics affects podosome belt formation during osteoclast induction.

    abstract::Osteoclast is the only cell that can degrade bone tissue in vivo. Recent studies have shown the important role of cytoskeleton dynamics in osteolysis and the formation of podosome belt in osteoclasts. This process is regulated by the dynamic microtubule (MT) network. We treated osteoclast precursor cells Raw264.7 with...

    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章

    doi:10.1007/s12013-014-0258-0

    authors: Ti Y,Zhou L,Wang R,Zhao J

    更新日期:2015-03-01 00:00:00

  • One-dimensional migration of olfactory ensheathing cells on synthetic materials: experimental and numerical characterization.

    abstract::Olfactory ensheathing cells (OECs) are of great interest for regenerative purposes since they are believed to aid axonal growth. With the view set on the strategies to achieve reconnection between neuronal structures, it is of great importance to characterize the behaviour of these cells on long thread-like structures...

    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章

    doi:10.1007/s12013-012-9399-1

    authors: Pérez-Garnés M,Martínez-Ramos C,Barcia JA,Escobar Ivirico JL,Gómez-Pinedo U,Vallés-Lluch A,Monleón Pradas M

    更新日期:2013-01-01 00:00:00

  • Protective effect of sRAGE on fetal development in pregnant rats with gestational diabetes mellitus.

    abstract::To investigate the protective effect of secretory receptor for advanced glycation endproducts (sRAGE) on the fetal development using rat model of gestational diabetes mellitus (GDM). The model of pregnant rats with intrauterine hyperglycemia was established by intraperitoneal injection of 25 mg/kg streptozotocin (STZ)...

    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章

    doi:10.1007/s12013-014-0233-9

    authors: Tang X,Qin Q,Xie X,He P

    更新日期:2015-03-01 00:00:00

  • Insulin receptors and downstream substrates associate with membrane microdomains after treatment with insulin or chromium(III) picolinate.

    abstract::We have examined the association of insulin receptors (IR) and downstream signaling molecules with membrane microdomains in rat basophilic leukemia (RBL-2H3) cells following treatment with insulin or tris(2-pyridinecarbxylato)chromium(III) (Cr(pic)(3)). Single-particle tracking demonstrated that individual IR on these...

    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章

    doi:10.1007/s12013-011-9326-x

    authors: Al-Qatati A,Winter PW,Wolf-Ringwall AL,Chatterjee PB,Van Orden AK,Crans DC,Roess DA,Barisas BG

    更新日期:2012-04-01 00:00:00

  • Non-esterified fatty acids activate the AMP-activated protein kinase signaling pathway to regulate lipid metabolism in bovine hepatocytes.

    abstract::Non-esterified fatty acids (NEFAs) act as signaling molecules involved in regulating genes expression to modulate lipid metabolism. However, the regulation mechanism of NEFAs on lipid metabolism in dairy cows is unclear. The AMP-activated protein kinase (AMPK) signaling pathway plays a key role in regulating hepatic l...

    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章

    doi:10.1007/s12013-013-9629-1

    authors: Li X,Li X,Chen H,Lei L,Liu J,Guan Y,Liu Z,Zhang L,Yang W,Zhao C,Fu S,Li P,Liu G,Wang Z

    更新日期:2013-01-01 00:00:00

  • Functions and roles of a protein-associated factor.

    abstract::The divergence of protein (Hedgehog) in different organisms remains an unresolved issue to comprehend how the pathway in Hh signaling evolves. Insights into this question can help one identify the key molecules in Hh signaling. This work proposes a protein-associated factor in cells. The development of a non-reduction...

    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章

    doi:10.1007/s12013-013-9743-0

    authors: Sarker MM,Zhou MC,Rameshwar P,Hanover JA

    更新日期:2014-04-01 00:00:00

  • Regulation of tetrahydrobiopterin synthesis and bioavailability in endothelial cells.

    abstract::Tetrahydrobiopterin (BH4) is a member of the pterin family that has a core structure of pyrazino-2,3-d-pyrimidine rings. Because BH4 is an essential cofactor for the biosynthesis of nitric oxide (a major vasodilator), there is growing interest in BH4 biochemistry in endothelial cells (the cells that line blood vessels...

    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章,评审

    doi:10.1385/CBB:41:3:415

    authors: Shi W,Meininger CJ,Haynes TE,Hatakeyama K,Wu G

    更新日期:2004-01-01 00:00:00

  • Acteoside Presents Protective Effects on Cerebral Ischemia/reperfusion Injury Through Targeting CCL2, CXCL10, and ICAM1.

    abstract::The objective of this study is to investigate the roles of acteoside (ACT) in cells with oxygen-glucose deprivation and reoxygenation (OGD/R)-induced injury and the underlying mechanisms. The differentially expressed genes (DEGs) in rats with middle cerebral artery occlusion were identified using GSE61616 data set. Ky...

    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章

    doi:10.1007/s12013-020-00965-8

    authors: Wu W,Wu G,Cao D

    更新日期:2021-01-13 00:00:00

  • On the Regularities of the Polar Profiles of Proteins Related to Ebola Virus Infection and their Functional Domains.

    abstract::The number of fatalities and economic losses caused by the Ebola virus infection across the planet culminated in the havoc that occurred between August and November 2014. However, little is known about the molecular protein profile of this devastating virus. This work represents a thorough bioinformatics analysis of t...

    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章

    doi:10.1007/s12013-018-0839-4

    authors: Polanco C,Samaniego Mendoza JL,Buhse T,Uversky VN,Bañuelos Chao IP,Bañuelos Cedano MA,Tavera FM,Tavera DM,Falconi M,Ponce de León AV

    更新日期:2018-09-01 00:00:00

  • Simultaneous Determination of Human Erythrocyte Deformability and Adhesion Energy: A Novel Approach Using a Microfluidic Chamber and the "Glass Effect".

    abstract::The simultaneous determination of adhesion and deformability parameters of erythrocytes was carried out through a microfluidic device, which uses an inverted optical microscope with new image acquisition and analysis technologies. Also, an update of the models describing erythrocyte adhesion and deformation was propos...

    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章

    doi:10.1007/s12013-020-00956-9

    authors: Londero CM,Riquelme BD

    更新日期:2020-11-07 00:00:00

  • Hemodiafiltration combined with resin-mediated absorption as a therapy for hyperlipidemic acute pancreatitis.

    abstract::The aim of this study is to investigate whether hemodiafiltration combined with resin-mediated absorption is a better therapy for hyperlipidemic acute pancreatitis. Patients (n = 67) with acute pancreatitis treated in ICU from January 2009 to December 2012 were included in this study. Seven of these 67 cases were diag...

    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章

    doi:10.1007/s12013-014-9855-1

    authors: Li MQ,Shi ZX,Xu JY,Lu B,Li JQ,Xu YJ,Wang XM,Li SM,Mo X

    更新日期:2014-07-01 00:00:00

  • Transarterial Chemoembolization of Giant Liver Haemangioma: A Multi-center Study with 836 Cases.

    abstract::The purpose of the study is to evaluate the effectiveness and safety of transarterial chemoembolization (TACE) with pingyangmycin-lipiodol emulsion for the treatment of symptomatic giant hepatic haemangioma. Four hospitals (The Second Hospital of Shandong University, Qilu Hospital of Shandong University, Shandong Prov...

    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章,多中心研究

    doi:10.1007/s12013-015-0680-y

    authors: Li Y,Jia Y,Li S,Wang W,Wang Z,Wang Y,Liu B,Wang W,Chang H,Li Z

    更新日期:2015-11-01 00:00:00

  • Unique structural changes in calcium-bound calmodulin upon interaction with protein 4.1R FERM domain: novel insights into the calcium-dependent regulation of 4.1R FERM domain binding to membrane proteins by calmodulin.

    abstract::Calmodulin (CaM) binds to the FERM domain of 80 kDa erythrocyte protein 4.1R (R30) independently of Ca(2+) but, paradoxically, regulates R30 binding to transmembrane proteins in a Ca(2+)-dependent manner. We have previously mapped a Ca(2+)-independent CaM-binding site, pep11 (A(264)KKLWKVCVEHHTFFR), in 4.1R FERM domai...

    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章

    doi:10.1007/s12013-013-9758-6

    authors: Nunomura W,Isozumi N,Nakamura S,Jinbo Y,Ohki S,Kidokoro S,Wakui H,Takakuwa Y

    更新日期:2014-05-01 00:00:00

  • Genotoxicity test of self-renovated ceramics in primary human peripheral lymphocytes.

    abstract::Zirconia-based ceramics is widely used in dentistry. Different compositions of ceramics have different features. Our self-renovated ceramics become more machinable without scarifying its dental restoration properties after adjusting ratio of lanthanum phosphate (LaPO4)/yttrium oxide (Y2O3). In order to evaluate its sa...

    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章

    doi:10.1007/s12013-014-0127-x

    authors: Hua N,Zhu H,Zhuang J,Chen L

    更新日期:2014-12-01 00:00:00

  • Transforming growth factor-beta1 and disorders of the lung.

    abstract::The transforming growth factor (TGF) superfamily encompasses about 30 members in mammals. The effect of TGF-beta subfamily members is exerted and regulated via selective pathways of synthesis and signaling that involve activation of latent TGF-beta, specific and high-affinity binding to cell membrane serine/threonine ...

    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章,评审

    doi:10.1385/CBB:43:1:131

    authors: Camoretti-Mercado B,Solway J

    更新日期:2005-01-01 00:00:00

  • Electrophoretic separation of ventricular myosin isoenzymes using a native polyacrylamide minigel system.

    abstract::A method is presented to separate rabbit cardiac ventricular myosin isoenzymes (V(1), V(2), V(3)), which are large and important contractile proteins. This polyacrylamide gel electrophoresis--using a slab minigel format--does not involve preparation of an acrylamide gradient or denaturing conditions. The isoenzyme mig...

    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章

    doi:10.1385/CBB:38:1:33

    authors: Garcia Pomblum SC,Pomblum VJ,Gams E,Reiser PJ,Schipke JD

    更新日期:2003-01-01 00:00:00

  • Comparison of Gene Expression Profile Between Tumor Tissue and Adjacent Non-tumor Tissue in Patients with Gastric Gastrointestinal Stromal Tumor (GIST).

    abstract::Gastrointestinal stromal tumors (GISTs) are defined as spindle cell and/or epithelioid tumors originated from interstitial Cajal cells or precursors in the digestive tract. This study was conducted to identify genes differing in expression between the gastric tumors and the adjacent non-cancerous mucosas in patients w...

    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章

    doi:10.1007/s12013-014-0504-5

    authors: Kou Y,Zhao Y,Bao C,Wang Q

    更新日期:2015-06-01 00:00:00

  • Sorting of peroxisomal and mitochondrial carnitine acetyltransferase isozymes in the diploid yeast, Candida tropicalis.

    abstract::Peroxisomal and mitochondrial carnitine acetyltransferase (CAT; EC 2.3.1.7) isozymes are synthesized from the first and second ATG codons of the open reading frame of one gene, Candida tropicalis CAT. Primer extension analysis and RNase protection assay revealed that the peroxisomal CAT, initiating at the second AUG c...

    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章

    doi:10.1385/cbb:32:1-3:139

    authors: Tanaka A,Ueda M

    更新日期:2000-01-01 00:00:00

  • Azithromycin attenuates fibroblast growth factors induced vascular endothelial growth factor via p38(MAPK) signaling in human airway smooth muscle cells.

    abstract::The airways in asthma and COPD are characterized by an increase in airway smooth muscle (ASM) mass and bronchial vascular changes associated with increased expression of pro-angiogenic growth factors, such as fibroblast growth factors (FGF-1 and FGF-2) and vascular endothelial growth factor (VEGF). We investigated the...

    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章

    doi:10.1007/s12013-011-9331-0

    authors: Willems-Widyastuti A,Vanaudenaerde BM,Vos R,Dilisen E,Verleden SE,De Vleeschauwer SI,Vaneylen A,Mooi WJ,de Boer WI,Sharma HS,Verleden GM

    更新日期:2013-11-01 00:00:00

  • Negative regulation of Janus kinases.

    abstract::The precise regulation of both the magnitude and the duration of Janus kinase (JAK) catalytic activity is essential for the cytokine orchestration of many biological processes, and the dysregulation of JAK activity has pathological implications. Immunosuppressive disease states, such as X-linked severe combined immuno...

    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章,评审

    doi:10.1385/CBB:34:1:17

    authors: Duhé RJ,Wang LH,Farrar WL

    更新日期:2001-01-01 00:00:00

  • Mechanical induction of BMP-7 in osteocyte blocks glucocorticoid-induced apoptosis through PI3K/AKT/GSK3β pathway.

    abstract::Mechanical loading is known to promote osteocyte survival, whereas glucocorticoid treatment results in osteocyte apoptosis. Here, we report that BMP-7, which was secreted by osteocyte in response to mechanical loading, exerts anti-apoptotic effect against dexamethasone-induced apoptosis of osteocytes. We further show ...

    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章

    doi:10.1007/s12013-013-9543-6

    authors: Wang Z,Guo J

    更新日期:2013-11-01 00:00:00

  • Anti-proliferative Effect of Physcion on Human Gastric Cell Line via Inducing ROS-Dependent Apoptosis.

    abstract::In this study, the anti-proliferative effect of physcion, an anthraquinone derivative isolated and characterized from both terrestrial and marine sources, against human gastric cancer SGC-7901 cells was investigated and the underlying mechanisms were explored. Physcion reduced SGC-7901 cell viability in a dose- and ti...

    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章

    doi:10.1007/s12013-015-0674-9

    authors: Xiong Y,Ren L,Wang Z,Hu Z,Zhou Y

    更新日期:2015-11-01 00:00:00

  • Jak2 tyrosine kinase: a true jak of all trades?

    abstract::Discovered roughly 10 yr ago, Jak2 tyrosine kinase has emerged as a critical molecule in mammalian development, physiology, and disease. Here, we review the early history of Jak2 and its role in health and disease. We will also review its critical role in mediating cytokine-dependent signal transduction. Additionally,...

    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章,评审

    doi:10.1385/cbb:41:2:207

    authors: Sandberg EM,Wallace TA,Godeny MD,VonDerLinden D,Sayeski PP

    更新日期:2004-01-01 00:00:00

  • Enteral ecoimmunonutrition reduced enteral permeability and serum ghrelin activity in severe cerebral stroke patients with lung infection.

    abstract::The study analyzed how enteral ecoimmunonutrition, which comprises probiotics, glutamine, fish oil, and Enteral Nutritional Suspension (TPF), can impact on the enteral permeability and serum Ghrelin activity in severe cerebral stroke patients with lung infection. Among 190 severe cerebral stroke patients with toleranc...

    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章

    doi:10.1007/s12013-014-0184-1

    authors: Xu XD,Shao F

    更新日期:2015-01-01 00:00:00