Biorheological action of Ascaris lumbricoides larvae on human erythrocytes.

Abstract:

:Previous studies have shown that A. lumbricoides extracts capture sialic acid (SA) from human red blood cells (RBC). The aim of this work was to study hemorheological alterations in vitro caused by parasite larvae. The biorheological action of three larva concentrates of first and second larval stage on group O erythrocytes was analyzed by incubating the erythrocyte packed together with an equal volume of larvae (treated RBC) and PBS (control RBC). Distribution and parameters of aggregation (digital image analysis), aggregation kinetics (erythroaggregameter), and viscoelasticity (erythrodeformeter) were measured. The digital image analysis showed that all the larvae diminished the isolated cells percentage and increased the size of the formed aggregates. The aggregate formation velocity was lower in the treated than in the control. The deformability index (ID) values of treated RBC did not present variations with respect to those of the control, but a decrease in the erythrocyte elastic modulus (μ(m)) and membrane surface viscosity (η(m)) values was observed, indicating that the larvae not only induced a diminution in the membrane surface viscosity of RBC but also altered the dynamic viscoelasticity of the membrane. Experiments carried out in vitro support the conclusion that the contact between larvae and RBC produces hemorheological alterations.

journal_name

Cell Biochem Biophys

authors

de León PP,Del Balzo G,Riquelme B

doi

10.1007/s12013-012-9425-3

subject

Has Abstract

pub_date

2013-03-01 00:00:00

pages

237-42

issue

2

eissn

1085-9195

issn

1559-0283

journal_volume

65

pub_type

杂志文章
  • Slow inactivation in voltage-gated sodium channels: molecular substrates and contributions to channelopathies.

    abstract::Slow inactivation in voltage-gated sodium channels is a biophysical process that governs the availability of sodium channels over extended periods of time. Slow inactivation, therefore, plays an important role in controlling membrane excitability, firing properties, and spike frequency adaptation. Defective slow inact...

    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章,评审

    doi:10.1385/CBB:35:2:171

    authors: Vilin YY,Ruben PC

    更新日期:2001-01-01 00:00:00

  • Transforming growth factor-beta1 and disorders of the lung.

    abstract::The transforming growth factor (TGF) superfamily encompasses about 30 members in mammals. The effect of TGF-beta subfamily members is exerted and regulated via selective pathways of synthesis and signaling that involve activation of latent TGF-beta, specific and high-affinity binding to cell membrane serine/threonine ...

    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章,评审

    doi:10.1385/CBB:43:1:131

    authors: Camoretti-Mercado B,Solway J

    更新日期:2005-01-01 00:00:00

  • Insulin receptors and downstream substrates associate with membrane microdomains after treatment with insulin or chromium(III) picolinate.

    abstract::We have examined the association of insulin receptors (IR) and downstream signaling molecules with membrane microdomains in rat basophilic leukemia (RBL-2H3) cells following treatment with insulin or tris(2-pyridinecarbxylato)chromium(III) (Cr(pic)(3)). Single-particle tracking demonstrated that individual IR on these...

    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章

    doi:10.1007/s12013-011-9326-x

    authors: Al-Qatati A,Winter PW,Wolf-Ringwall AL,Chatterjee PB,Van Orden AK,Crans DC,Roess DA,Barisas BG

    更新日期:2012-04-01 00:00:00

  • Differential expression of dicer, miRNAs, and inflammatory markers in diabetic Ins2+/- Akita hearts.

    abstract::Diabetic cardiomyopathy is a leading cause of morbidity and mortality, and Insulin2 mutant (Ins2+/-) Akita is a genetic mice model of diabetes relevant to humans. Dicer, miRNAs, and inflammatory cytokines are associated with heart failure. However, the differential expression of miRNAs, dicer, and inflammatory molecul...

    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章

    doi:10.1007/s12013-013-9679-4

    authors: Chavali V,Tyagi SC,Mishra PK

    更新日期:2014-01-01 00:00:00

  • Characterization of 3-iodothyronamine in vitro dynamics by mathematical modeling.

    abstract::3-Iodothyronamine (T1AM) is regarded as a hormone-like substance thanks to its endogenous nature, its interaction with specific receptors trace amine-associated receptor 1 and its biological effects. We characterized T1AM transport and conversion in an in vitro culture of H9c2 murine cells, after a T1AM bolus injectio...

    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章

    doi:10.1007/s12013-013-9680-y

    authors: Orsi G,Ghelardoni S,Saba A,Zucchi R,Vozzi G

    更新日期:2014-01-01 00:00:00

  • Determination of DNA damage in experimental liver intoxication and role of N-acetyl cysteine.

    abstract::The present study aimed at detecting DNA damage and fragmentation as well as histone acetylation depending on oxidative stress caused by CCl4 intoxication. Also, the protective role of N-acetyl cysteine, a precursor for GSH, in DNA damage is investigated. Sixty rats were used in this study. In order to induce liver to...

    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章

    doi:10.1007/s12013-014-0031-4

    authors: Aksit H,Bildik A

    更新日期:2014-11-01 00:00:00

  • Molecular mechanisms by which saturated fatty acids modulate TNF-α expression in mouse macrophage lineage.

    abstract::Many macrophage functions are modulated by fatty acids (FAs), including cytokine release, such as tumor necrosis factor-α (TNF-α). TNF-α is of great interest due to its role in the inflammation process observed in several diseases such as rheumatoid arthritis, atherosclerosis, and obesity. However, the mechanisms by w...

    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章

    doi:10.1007/s12013-010-9117-9

    authors: de Lima-Salgado TM,Alba-Loureiro TC,do Nascimento CS,Nunes MT,Curi R

    更新日期:2011-03-01 00:00:00

  • Effect of sulforaphane on growth inhibition in human brain malignant glioma GBM 8401 cells by means of mitochondrial- and MEK/ERK-mediated apoptosis pathway.

    abstract::In recent studies, sulforaphane (SFN) has been seen to demonstrate antioxidant and anti-tumor activities as well as potent chemopreventive action against cancer. The present study investigates the anti-proliferation (using MTT assay, SFN demonstrated cytotoxic activity against GBM 8401 cell with IC(50) values at 35.52...

    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章

    doi:10.1007/s12013-012-9360-3

    authors: Huang TY,Chang WC,Wang MY,Yang YR,Hsu YC

    更新日期:2012-07-01 00:00:00

  • Introducing a New Model of Sweet Taste Receptor, a Class C G-protein Coupled Receptor (C GPCR).

    abstract::The structure of sweet taste receptor (STR), a heterodimer of class C G-protein coupled receptors comprising T1R2 and T1R3 molecules, is still undetermined. In this study, a new enhanced model of the receptor is introduced based on the most recent templates. The improvement, stability, and reliability of the model are...

    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章

    doi:10.1007/s12013-019-00872-7

    authors: Kashani-Amin E,Sakhteman A,Larijani B,Ebrahim-Habibi A

    更新日期:2019-09-01 00:00:00

  • Secreted phosphoprotein 1 upstream invasive network construction and analysis of lung adenocarcinoma compared with human normal adjacent tissues by integrative biocomputation.

    abstract::The aim of this study is to set up single molecular secreted phosphoprotein 1 (SPP1) upstream invasive network of lung adenocarcinoma. This paper proposed an integrated method based on linear programming and a decomposition procedure with integrated analysis of the significant function cluster using Kappa statistics a...

    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章

    doi:10.1007/s12013-009-9071-6

    authors: Sun Y,Wang L,Jiang M,Huang J,Liu Z,Wolfl S

    更新日期:2010-04-01 00:00:00

  • Novel c.2216T > C (p.I739T) mutation in exon 13 and c.1481T > A (p.L494X) mutation in exon 8 of MUT gene in a female with methylmalonic acidemia.

    abstract::We report herein a 1.5-year-old girl with methylmalonic acidemia (MMA) in whom two missense mutations were found: a novel I739T mutation located in exon 13 and the L494X mutation in exon 8. The results of organic acid test showed a pronounced increase in methylmalonate excretion with increased methylcitrate and 3-OH-p...

    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章

    doi:10.1007/s12013-013-9532-9

    authors: Imataka G,Sakamoto O,Yamanouchi H,Yoshihara S,Omura-Hasegawa Y,Tajima G,Arisaka O

    更新日期:2013-09-01 00:00:00

  • Study about the effects of different fitness sports on cognitive function and emotion of the aged.

    abstract::The aim of the study was to explore the effects of different fitness sports on cognitive function and emotion of the aged people. A total of 150 subjects aged between 60 and 70 were recruited from Shenyang Aged University and elderly activity center. All subjects reported no fitness before this study. The aged subject...

    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章,随机对照试验

    doi:10.1007/s12013-014-0100-8

    authors: Zhang X,Ni X,Chen P

    更新日期:2014-12-01 00:00:00

  • Influence of amino acids Shiff bases on irradiated DNA stability in vivo.

    abstract::To reveal protective role of the new Mn(II) complexes with Nicotinyl-L-Tyrosinate and Nicotinyl-L-Tryptophanate Schiff Bases against ionizing radiation. The DNA of the rats liver was isolated on 7, 14, and 30 days after X-ray irradiation. The differences between the DNA of irradiated rats and rats pre-treated with Mn(...

    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章

    doi:10.1007/s12013-013-9617-5

    authors: Karapetyan NH,Malakyan MH,Bajinyan SA,Torosyan AL,Grigoryan IE,Haroutiunian SG

    更新日期:2013-01-01 00:00:00

  • Curcumin Promotes Cell Cycle Arrest and Inhibits Survival of Human Renal Cancer Cells by Negative Modulation of the PI3K/AKT Signaling Pathway.

    abstract::Curcumin possesses anti-cancer effects. In the current study, we tested the effect of curcumin on cell proliferation, viability, apoptosis, cell cycle phases, and activation of the PI3K/Akt pathway in the renal cell carcinoma (RCC) cell line RCC-949. We observed that cell proliferation and viability were markedly inhi...

    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章

    doi:10.1007/s12013-015-0694-5

    authors: Zhang H,Xu W,Li B,Zhang K,Wu Y,Xu H,Wang J,Zhang J,Fan R,Wei J

    更新日期:2015-12-01 00:00:00

  • Uptake of reconstituted Na,K-ATPase vesicles by isolated lymphocytes measured by FACS, confocal microscopy and spectrofluorometry.

    abstract::Na,K-ATPase (EC 3.6.1.37, Na,K-ATPase) is a fundamental vital membrane transport and receptor system which, after biosynthesis, is exported to the plasma membrane in inside-out vesicles. Na,K-ATPase can be extracted form the natural membrane and inserted into artificially formed phosphatidylcholine vesicles (liposomes...

    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章

    doi:10.1007/BF02738123

    authors: Anner BM,Volet B

    更新日期:1999-01-01 00:00:00

  • Selective autophagy: talking with the UPS.

    abstract::Far from now are the days when investigators raced to identify the proteolytic system responsible for the degradation of their favorite protein. Nowadays, it is well accepted that a given protein can be degraded by different systems depending on factors such as cell type, cellular conditions, or functionality of each ...

    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章,评审

    doi:10.1007/s12013-013-9623-7

    authors: Park C,Cuervo AM

    更新日期:2013-09-01 00:00:00

  • Combined hepatocellular-cholangiocarcinoma with fever of unknown origin: a case report and review of literature.

    abstract::Combined hepatocellular carcinoma and cholangiocarcinoma (cHCC-CC) is a rare form of primary liver cancer (PLC). It is difficult to make a correct preoperative diagnosis of cHCC-CC because of the lack of special features of the disease. We here present a case of a 68-year-old man who presented with fluctuant fever, ch...

    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章

    doi:10.1007/s12013-013-9760-z

    authors: E C,Xie Y,Yang Y,Ji D,Li W,Zhang X

    更新日期:2014-05-01 00:00:00

  • Alteration in the balance of prosurvival and proapoptotic signalling pathways leads to sequence-dependent synergism between docetaxel and sorafenib in human non-small cell lung cancer cell lines.

    abstract::To examine the antiproliferative effect of the combination of docetaxel and sorafenib, applied to the representative non-small cell lung cancer cell line A549 cells either wild type or with acquired resistance to docetaxel (A549/D). The aim of this study is to evaluate the synergistic effect of combination treatment o...

    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章

    doi:10.1007/s12013-013-9722-5

    authors: He X,Zhang T

    更新日期:2014-03-01 00:00:00

  • Regulation of the Pleuronectes americanus Na+/H+ exchanger by osmotic shrinkage, beta-adrenergic stimuli, and inhibition of Ser/Thr protein phosphatases.

    abstract::The ubiquitous Na+/H+ exchanger NHE1 is regulated by protein phosphorylation events, but the mechanisms involved are incompletely understood. We recently cloned NHE1 from the red blood cells of the winter flounder, Pleuronectes americanus (paNHE1), and demonstrated its activation by osmotic cell shrinkage, beta-adrene...

    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章

    doi:10.1385/CBB:45:1:1

    authors: Holt ME,King SA,Cala PM,Pedersen SF

    更新日期:2006-01-01 00:00:00

  • Analysis of cytotoxic T lymphocyte antigen-4 (CTLA-4) promoter -318C/T and +49A/G gene polymorphisms in Turkish patients with familial Mediterranean fever.

    abstract::Either the role of the adaptive immune system or the interaction between innate and adaptive immune systems in familial Mediterranean fever (FMF) is not clear so far. So, we planned to search for the interaction between the innate and adaptive immune systems in the pathogenesis of FMF by investigating polymorphism for...

    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章

    doi:10.1007/s12013-012-9416-4

    authors: Gunesacar R,Erken E,Dinkci S

    更新日期:2013-03-01 00:00:00

  • Sorting of peroxisomal and mitochondrial carnitine acetyltransferase isozymes in the diploid yeast, Candida tropicalis.

    abstract::Peroxisomal and mitochondrial carnitine acetyltransferase (CAT; EC 2.3.1.7) isozymes are synthesized from the first and second ATG codons of the open reading frame of one gene, Candida tropicalis CAT. Primer extension analysis and RNase protection assay revealed that the peroxisomal CAT, initiating at the second AUG c...

    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章

    doi:10.1385/cbb:32:1-3:139

    authors: Tanaka A,Ueda M

    更新日期:2000-01-01 00:00:00

  • Extracting the stepping dynamics of molecular motors in living cells from trajectories of single particles.

    abstract::Molecular motors are responsible of transporting a wide variety of cargos in the cytoplasm. Current efforts are oriented to characterize the biophysical properties of motors in cells with the aim of elucidating the mechanisms of these nanomachines in the complex cellular environment. In this study, we present an algor...

    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章

    doi:10.1007/s12013-012-9397-3

    authors: Bruno A,Bruno L,Levi V

    更新日期:2013-01-01 00:00:00

  • Posterior fossa decompression combined with resection of the cerebellomedullary fissure membrane and expansile duraplasty: a radical and rational surgical treatment for Arnold-Chiari type I malformation.

    abstract::This study aims to introduce a new surgical procedure for the treatment of Arnold-Chiari type I malformation (ACM-1) and to compare its effectiveness with the techniques described in previous studies. We performed the following procedures: foramen magnum decompression combined with the removal of both the atlanto-occi...

    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章

    doi:10.1007/s12013-014-0135-x

    authors: Liang CJ,Dong QJ,Xing YH,Shan M,Wen LX,Qiang ZY,Ping ZQ,Tao PZ,Ping HX

    更新日期:2014-12-01 00:00:00

  • Association of hematocrit and pre-hypertension among Chinese adults: the CRC study.

    abstract::Elevated blood pressure is regarded as an independent risk factor for cardiovascular diseases and diabetes. We examined the relation between hematocrit and pre-hypertension as well as the effect of sex, obesity, fasting glucose, and lipids in Chinese adults. The study samples were from a community-based health examina...

    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章

    doi:10.1007/s12013-014-0318-5

    authors: Liu X,Liang J,Qiu Q,Zhu Y,Sun Y,Ying P,Teng F,Wang Y,Qi L

    更新日期:2015-03-01 00:00:00

  • EPR Characterization of Dinitrosyl Iron Complexes with Thiol-Containing Ligands as an Approach to Their Identification in Biological Objects: An Overview.

    abstract::The overview demonstrates how the use of only one physico-chemical approach, viz., the electron paramagnetic resonance method, allowed detection and identification of dinitrosyl iron complexes with thiol-containing ligands in various animal and bacterial cells. These complexes are formed in biological objects in the p...

    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章,评审

    doi:10.1007/s12013-017-0811-8

    authors: Vanin AF

    更新日期:2018-06-01 00:00:00

  • Ion exchangers mediating NaCl transport in the renal proximal tubule.

    abstract::We have studied the mechanisms of NaCl transport in the mammalian proximal tubule. Studies of isolated brush-border membrane vesicles confirmed the presence of Na+-H+ exchange and identified Cl(-)-formate and Cl(-)-oxalate exchangers as possible mechanisms of uphill Cl- entry. We found that formate and oxalate each st...

    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章,评审

    doi:10.1385/CBB:36:2-3:147

    authors: Aronson PS

    更新日期:2002-01-01 00:00:00

  • Mitochondrial P-glycoprotein ATPase contributes to insecticide resistance in the cotton bollworm, Helicoverpa armigera (Noctuidae: Lepidoptera).

    abstract::Cotton bollworm, Helicoverpa armigera, is one of the most damaging polyphagous pests worldwide, which has developed high levels of resistance to commonly applied insecticides. Mitochondrial P-glycoprotein (Pgp) was detected in the insecticide-resistant strain of H. armigera using C219 antibodies, and its possible role...

    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章

    doi:10.1007/s12013-014-9969-5

    authors: Akbar SM,Aurade RM,Sharma HC,Sreeramulu K

    更新日期:2014-09-01 00:00:00

  • (Pro)Insulin processing: a historical perspective.

    abstract::Insulin, the major secreted product of the beta-cells of the islets of Langerhans, is initially synthesized as a precursor (preproinsulin), from which the mature hormone is excised by a series of proteolytic cleavages. This review provides a personal narrative of some of the key research projects leading to the identi...

    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章,评审

    doi:10.1385/cbb:40:3:143

    authors: Davidson HW

    更新日期:2004-01-01 00:00:00

  • The effect of stromal cell-derived factor 1 in the migration of neural stem cells.

    abstract::Neural stem cells (NSCs) have widely been used in the treatment of human neurological disorders as cell therapy via intracerebral or intraventricular infusion. However, the migration mechanism required for NSCs homing and recruitment remains to be elucidated. Recently, SDF-1/CXCR4 axis was shown to be responsible for ...

    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章

    doi:10.1007/s12013-014-0103-5

    authors: Xue L,Wang J,Wang W,Yang Z,Hu Z,Hu M,Ding P

    更新日期:2014-12-01 00:00:00

  • Correlations between CT perfusion parameters and vascular endothelial growth factor expression and microvessel density in implanted VX2 lung tumors.

    abstract::To evaluate the correlation between CT perfusion parameters and tumor angiogenesis, expression of VEGF is implanted in VX2 lung tumor. VX2 tumor cells were injected in 15 healthy New Zealand rabbits. After 20 days, tumors with diameter over 7 mm were scanned with 16 row spiral CT. Using Software Perfusion 3, blood flo...

    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章

    doi:10.1007/s12013-014-9966-8

    authors: Ling S,Deng D,Mo Y,Zhang X,Guan X,Wei Q

    更新日期:2014-09-01 00:00:00