Characterization of 3-iodothyronamine in vitro dynamics by mathematical modeling.


:3-Iodothyronamine (T1AM) is regarded as a hormone-like substance thanks to its endogenous nature, its interaction with specific receptors trace amine-associated receptor 1 and its biological effects. We characterized T1AM transport and conversion in an in vitro culture of H9c2 murine cells, after a T1AM bolus injection. Samples of cell medium culture and cell lysate were assayed by high-performance liquid chromatography coupled to tandem mass spectrometry. We performed comparative experiments by adding to T1AM bolus amino oxidase inhibitors as iproniazid, pargyline (monoamine oxidase, MAO inhibitors), aminoguanidine, and semicarbazide (semicarbazide-sensitive amino oxidase, SSAO inhibitors). A mathematical model was developed, based on the assumption that T1AM is transported with a mechanism that is typical of hormone transport (i.e., EGF or insulin). We noticed that surface receptors downregulation could play a major role in T1AM dynamics. We also estimated that T1AM catabolism is mainly affected by MAO inhibitors, which produce a dramatic decrease in the kinetic constants related to T1AM degradation, while no significant changes were observed in experiments with SSAO inhibitors.


Cell Biochem Biophys


Orsi G,Ghelardoni S,Saba A,Zucchi R,Vozzi G




Has Abstract


2014-01-01 00:00:00












  • Transarterial Chemoembolization of Giant Liver Haemangioma: A Multi-center Study with 836 Cases.

    abstract::The purpose of the study is to evaluate the effectiveness and safety of transarterial chemoembolization (TACE) with pingyangmycin-lipiodol emulsion for the treatment of symptomatic giant hepatic haemangioma. Four hospitals (The Second Hospital of Shandong University, Qilu Hospital of Shandong University, Shandong Prov...

    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章,多中心研究


    authors: Li Y,Jia Y,Li S,Wang W,Wang Z,Wang Y,Liu B,Wang W,Chang H,Li Z

    更新日期:2015-11-01 00:00:00

  • Ion exchangers mediating NaCl transport in the renal proximal tubule.

    abstract::We have studied the mechanisms of NaCl transport in the mammalian proximal tubule. Studies of isolated brush-border membrane vesicles confirmed the presence of Na+-H+ exchange and identified Cl(-)-formate and Cl(-)-oxalate exchangers as possible mechanisms of uphill Cl- entry. We found that formate and oxalate each st...

    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章,评审


    authors: Aronson PS

    更新日期:2002-01-01 00:00:00

  • Non-esterified fatty acids activate the AMP-activated protein kinase signaling pathway to regulate lipid metabolism in bovine hepatocytes.

    abstract::Non-esterified fatty acids (NEFAs) act as signaling molecules involved in regulating genes expression to modulate lipid metabolism. However, the regulation mechanism of NEFAs on lipid metabolism in dairy cows is unclear. The AMP-activated protein kinase (AMPK) signaling pathway plays a key role in regulating hepatic l...

    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章


    authors: Li X,Li X,Chen H,Lei L,Liu J,Guan Y,Liu Z,Zhang L,Yang W,Zhao C,Fu S,Li P,Liu G,Wang Z

    更新日期:2013-01-01 00:00:00

  • Expression and localization of estrogen receptor in human breast cancer and its clinical significance.

    abstract::The aim of this study was to analyze the clinicopathologic significance of ERα protein that localized in the cell cytoplasm and/or cell membrane of human breast cancer and explore what kind of protein that the cytoplasm/membrane ERα belongs to. ERα expressions in 61 cases of breast cancer are detected by immunohistoch...

    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章


    authors: Li L,Wang Q,Lv X,Sha L,Qin H,Wang L,Li L

    更新日期:2015-01-01 00:00:00

  • Implementation of a fast-track clinical pathway decreases postoperative length of stay and hospital charges for liver resection.

    abstract::A fast-track clinical pathway is designed to streamline patient care delivery and maximize cost effectiveness. It has decreased postoperative length of stay (LOS) and hospital charges for many surgical procedures. However, data on clinical pathways after liver surgery are sparse. This study examined whether use of a f...

    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章


    authors: Lin DX,Li X,Ye QW,Lin F,Li LL,Zhang QY

    更新日期:2011-11-01 00:00:00

  • Brushes, cables, and anchors: recent insights into multiscale assembly and mechanics of cellular structural networks.

    abstract::The remarkable ability of living cells to sense, process, and respond to mechanical stimuli in their environment depends on the rapid and efficient interconversion of mechanical and chemical energy at specific times and places within the cell. For example, application of force to cells leads to conformational changes ...

    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章,评审


    authors: Lele TP,Kumar S

    更新日期:2007-01-01 00:00:00

  • Hemodiafiltration combined with resin-mediated absorption as a therapy for hyperlipidemic acute pancreatitis.

    abstract::The aim of this study is to investigate whether hemodiafiltration combined with resin-mediated absorption is a better therapy for hyperlipidemic acute pancreatitis. Patients (n = 67) with acute pancreatitis treated in ICU from January 2009 to December 2012 were included in this study. Seven of these 67 cases were diag...

    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章


    authors: Li MQ,Shi ZX,Xu JY,Lu B,Li JQ,Xu YJ,Wang XM,Li SM,Mo X

    更新日期:2014-07-01 00:00:00

  • Influence of Val108/158Met COMT Gene Polymorphism on the Efficacy of Modified Electroconvulsive Therapy in Patients with Treatment Resistant Depression.

    abstract::Depression is a common emotional disorder associated with increased risk of suicide and rate of disability. In this double-blinded control study, we tested the efficacy of modified electroconvulsive therapy (MECT) in patients with treatment resistant depression (TRD) using the Hamilton Depression Rating Scale for Depr...

    journal_title:Cell biochemistry and biophysics

    pub_type: 临床试验,杂志文章


    authors: Lin Z,He H,Zhang C,Wang Z,Jiang M,Li Q,Lan X,Zhang M,Huang X

    更新日期:2015-04-01 00:00:00

  • Differentiation of reprogrammed mouse cardiac fibroblasts into functional cardiomyocytes.

    abstract::Fibroblasts can be reprogrammed by ectopic expression of reprogramming factors to yield induced pluripotent stem (iPS) cells that are capable of transdifferentiating into diverse types of somatic cell lines. In this study, we examined if functional cardiomyocytes (CMs) can be produced from mouse cardiac fibroblasts (C...

    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章


    authors: Jiang B,Dong H,Li Q,Yu Y,Zhang Z,Zhang Y,Wang G,Zhang Z

    更新日期:2013-06-01 00:00:00

  • Spectral CT imaging in patients with Budd-Chiari syndrome: investigation of image quality.

    abstract::To assess the image quality of monochromatic imaging from spectral CT in patients with Budd-Chiari syndrome (BCS), fifty patients with BCS underwent spectral CT to generate conventional 140 kVp polychromatic images (group A) and monochromatic images, with energy levels from 40 to 80, 40 + 70, and 50 + 70 keV fusion im...

    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章


    authors: Su L,Dong J,Sun Q,Liu J,Lv P,Hu L,Yan L,Gao J

    更新日期:2014-11-01 00:00:00

  • Cyclic ADP-ribose and the regulation of calcium-induced calcium release in eggs and cardiac myocytes.

    abstract::Cyclic ADP-ribose (cADPR) is a cyclic metabolite of NAD+ synthesised in cells and tissues expressing ADP-ribosyl cyclases. Although it was first discovered in sea-urchin egg extracts as a potent calcium mobilizing agent, subsequent studies have indicated that it may have a widespread action in the activation of calciu...

    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章,评审


    authors: Galione A,Cui Y,Empson R,Iino S,Wilson H,Terrar D

    更新日期:1998-01-01 00:00:00

  • Crystal structure of the his-tagged saccharopine reductase from Saccharomyces cerevisiae at 1.7-A resolution.

    abstract::The three-dimensional structure of the saccharopine reductase enzyme from the budding yeast Saccharomyces cerevisiae was determined to 1.7-A resolution in the apo form by using molecular replacement. The enzyme monomer consists of three domains: domain I is a variant of the Rossmann fold, domain II folds into a alpha/...

    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章


    authors: Andi B,Cook PF,West AH

    更新日期:2006-01-01 00:00:00

  • Screening of Inherited Metabolic Disorders in Infants with Infantile Spasms.

    abstract::The objective of this study is to explore the incidence of inherited metabolic disorders (IMD) in infants with infantile spasms (IS), with an attempt to improve the early diagnosis and etiological and symptomatic treatment. Urine and blood samples were collected from 60 IS patients and analyzed for the quantification ...

    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章


    authors: Liu XM,Li R,Chen SZ,Sang Y,Chen J,Fan CH

    更新日期:2015-05-01 00:00:00

  • An observational study of autologous bone marrow-derived stem cells transplantation in seven patients with nervous system diseases: a 2-year follow-up.

    abstract::Currently, autologous bone marrow-derived stem cell is one of the most innovative areas of stem cells research. Previous studies on animal models of nervous system diseases have shown that these cells have a good effect on nervous system disorders. The alternative treatment with stem cells for the nervous system disea...

    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章


    authors: Ren C,Geng RL,Ge W,Liu XY,Chen H,Wan MR,Geng DQ

    更新日期:2014-05-01 00:00:00

  • Principles of macromolecular organization and cell function in bacteria and archaea.

    abstract::Structural organization of the cytoplasm by compartmentation is a well established fact for the eukaryotic cell. In prokaryotes, compartmentation is less obvious. Most prokaryotes do not need intracytoplasmic membranes to maintain their vital functions. This review, especially dealing with prokaryotes, will point out ...

    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章,评审


    authors: Hoppert M,Mayer F

    更新日期:1999-01-01 00:00:00

  • Molecular mechanisms by which saturated fatty acids modulate TNF-α expression in mouse macrophage lineage.

    abstract::Many macrophage functions are modulated by fatty acids (FAs), including cytokine release, such as tumor necrosis factor-α (TNF-α). TNF-α is of great interest due to its role in the inflammation process observed in several diseases such as rheumatoid arthritis, atherosclerosis, and obesity. However, the mechanisms by w...

    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章


    authors: de Lima-Salgado TM,Alba-Loureiro TC,do Nascimento CS,Nunes MT,Curi R

    更新日期:2011-03-01 00:00:00

  • Modelling the role of intrinsic electric fields in microtubules as an additional control mechanism of bi-directional intracellular transport.

    abstract::Active transport is essential for cellular function, while impaired transport has been linked to diseases such as neuronal degeneration. Much long distance transport in cells uses opposite polarity molecular motors of the kinesin and dynein families to move cargos along microtubules. It is clear that many types of car...

    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章


    authors: Sataric MV,Budinski-Petkovic L,Loncarevic I,Tuszynski JA

    更新日期:2008-01-01 00:00:00

  • Cardioprotective efficacy of a novel antioxidant mix VitaePro against ex vivo myocardial ischemia-reperfusion injury.

    abstract::Circumstantial evidence frequently implicates oxygen-derived free radicals and oxidative stress as mediators of myocardial ischemia/reperfusion (I/R) injury. Therefore, external supplementation of natural antioxidants plays a main role as cardioprotective compounds. This study was designed to evaluate the cardioprotec...

    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章


    authors: Adluri RS,Thirunavukkarasu M,Zhan L,Maulik N,Svennevig K,Bagchi M,Maulik G

    更新日期:2013-11-01 00:00:00

  • Insight into the oseltamivir resistance R292K mutation in H5N1 influenza virus: a molecular docking and molecular dynamics approach.

    abstract::H5N1 is a subtype of the influenza A virus that can cause disease in humans and many other animal species. Oseltamivir (Tamiflu) is a potent and selective antiviral drug employed to fight the flu virus in infected individuals by inhibiting neuraminidase (NA), a flu protein responsible for the release and spread of the...

    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章


    authors: Karthick V,Ramanathan K

    更新日期:2014-03-01 00:00:00

  • Homocysteine-induced apoptosis in endothelial cells coincides with nuclear NOX2 and peri-nuclear NOX4 activity.

    abstract::Apoptosis of endothelial cells related to homocysteine (Hcy) has been reported in several studies. In this study, we evaluated whether reactive oxygen species (ROS)-producing signaling pathways contribute to Hcy-induced apoptosis induction, with specific emphasis on NADPH oxidases. Human umbilical vein endothelial cel...

    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章


    authors: Sipkens JA,Hahn N,van den Brand CS,Meischl C,Cillessen SA,Smith DE,Juffermans LJ,Musters RJ,Roos D,Jakobs C,Blom HJ,Smulders YM,Krijnen PA,Stehouwer CD,Rauwerda JA,van Hinsbergh VW,Niessen HW

    更新日期:2013-11-01 00:00:00

  • Vitamin E induces phosphatidylserine externalization and red cell adhesion to endothelial cells.

    abstract::Red blood cell (RBC) adhesion to vessel wall endothelium is a potent catalyst of vascular occlusion and occurs in oxidative stress states such as hemoglobinopathies and cardiovascular conditions. These are often treated with vitamin E (VitE), a "classic" antioxidant. In this study, we examined the effects of VitE on R...

    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章


    authors: Koshkaryev A,Barshtein G,Yedgar S

    更新日期:2010-04-01 00:00:00

  • Experiences of microsurgical reconstruction for variant hepatic artery in living donor liver transplantation.

    abstract::There is an emergent need for improving the microsurgical technique of variant arterial anastomosis to reduce the often seen surgery-related complications. We describe in this article our experience in improving this technique, in 73 living donor liver grafts (64 right lobes, 9 left lobes) in patients with end-stage l...

    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章


    authors: Liang Y,Ye S,Shi X,Ji W,Duan W,Luo Y,Dong J

    更新日期:2013-03-01 00:00:00

  • Cell contraction caused by microtubule disruption is accompanied by shape changes and an increased elasticity measured by scanning acoustic microscopy.

    abstract::The state of crosslinking of microfilaments and the state of myosin-driven contraction are the main determinants of the mechanical properties of the cell cortex underneath the membrane, which is significant for the mechanism of shaping cells. Therefore, any change in the contractile state of the actomyosin network wou...

    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章


    authors: Karl I,Bereiter-Hahn J

    更新日期:1998-01-01 00:00:00

  • Influence of topoisomerase II inhibitors and ionizing radiation on growth arrest and cell death pathways in the breast tumor cell.

    abstract::Promotion of apoptosis (which is frequently dependent on functional p53) is thought to be critical for the effectiveness of chemotherapy or radiotherapy. Studies in this as well as other laboratories have demonstrated that breast tumor cells are relatively refractory to apoptosis in response to modalities that induce ...

    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章,评审


    authors: Gewirtz DA,Sundaram S,Magnet KJ

    更新日期:2000-01-01 00:00:00

  • Therapeutical effects and mechanism of salubrinal combined with ulinastatin on treating paraquat poisoning.

    abstract::To explore therapeutic effects and underlying mechanism of Salubrinal combined with Ulinastatin (UTI) on acute Paraquat (PQ) poisoning. Four hundred rats were randomly allocated into UTI group, SAL group, SAL + UTI and control group according to random number table with 100 rats in each group. Acute PQ poisoning model...

    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章


    authors: Guo H,Jiang C,Sun X

    更新日期:2014-12-01 00:00:00

  • The significance and mechanism of propofol on treatment of ischemia reperfusion induced lung injury in rats.

    abstract::This study is aimed to investigate the efficacy and underlying the mechanism of propofol in treatment of ischemia reperfusion (IR)-induced lung injury in rats, providing a novel insight of therapeutic strategy for IR-induced lung injury. 120 healthy SD rats were selected and randomly divided into sham operation group,...

    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章


    authors: Yang P,Yang N,Zhang X,Xu X

    更新日期:2014-12-01 00:00:00

  • Endocardial endothelial cells stimulate proliferation and collagen synthesis of cardiac fibroblasts.

    abstract::Given that vascular endothelial cells play an important role in the modulation of vascular structure and function, we hypothesized that endocardial endothelial cells (EECs) may have a modulator role in regulating the cardiac interstitial cells. Endocardial endothelial cells were isolated from freshly collected pig hea...

    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章


    authors: Kuruvilla L,Nair RR,Umashankar PR,Lal AV,Kartha CC

    更新日期:2007-01-01 00:00:00

  • Activation of p38 MAPK pathway by hepatitis C virus E2 in cells transiently expressing DC-SIGN.

    abstract::Dendritic cell-specific intercellular adhesion molecule 3-grabbing nonintegrin (DC-SIGN) is a cellular receptor for hepatitis C virus for the binding of viral envelope glycoprotein E2. Interaction of DC-SIGN with the E2 may evoke cellular signal transduction implicated in viral pathogenesis. We developed a cell model ...

    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章


    authors: Chen QL,Zhu SY,Bian ZQ,Zhao LJ,Cao J,Pan W,Qi ZT

    更新日期:2010-01-01 00:00:00

  • Expression and functional significance of ezrin in human brain astrocytoma.

    abstract::Ezrin is overexpressed in a variety of neoplastic cells and is involved in the later stages of tumor progression and metastasis. The present study investigated the expression and functional significance of ezrin in human brain astrocytoma. Ezrin expression was examined in specimens from healthy human brains (10 autops...

    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章


    authors: Mao J,Yuan XR,Xu SS,Jiang XC,Zhao XT

    更新日期:2013-01-01 00:00:00

  • The effect of stromal cell-derived factor 1 in the migration of neural stem cells.

    abstract::Neural stem cells (NSCs) have widely been used in the treatment of human neurological disorders as cell therapy via intracerebral or intraventricular infusion. However, the migration mechanism required for NSCs homing and recruitment remains to be elucidated. Recently, SDF-1/CXCR4 axis was shown to be responsible for ...

    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章


    authors: Xue L,Wang J,Wang W,Yang Z,Hu Z,Hu M,Ding P

    更新日期:2014-12-01 00:00:00