Abstract：
:Molecular dynamics simulations, binding free energy calculations, principle component analysis (PCA), and residue interaction network analysis were employed in order to investigate the molecular mechanism of M184I single mutation which played pivotal role in making the HIV-1 reverse transcriptase (RT) totally resistant to lamivudine. Results showed that single mutations at residue 184 of RT caused (1) distortion of the orientation of lamivudine in the active site due to the steric conflict between the oxathiolane ring of lamivudine and the side chain of beta-branched amino acids Ile at position 184 which, in turn, perturbs inhibitor binding, (2) decrease in the binding affinity by (~8 kcal/mol) when compared to the wild-type, (3) variation in the overall enzyme motion as evident from the PCA for both systems, and (4) distortion of the hydrogen bonding network and atomic interactions with the inhibitor. The comprehensive analysis presented in this report can provide useful information for understanding the drug resistance mechanism against lamivudine. The results can also provide some potential clues for further design of novel inhibitors that are less susceptible to drug resistance.
journal_name
Cell Biochem Biophysjournal_title
Cell biochemistry and biophysicsauthors
Moonsamy S,Bhakat S,Walker RC,Soliman MEdoi
10.1007/s12013-015-0709-2subject
Has Abstractpub_date
2016-03-01 00:00:00pages
35-48issue
1eissn
1085-9195issn
1559-0283pii
10.1007/s12013-015-0709-2journal_volume
74pub_type
杂志文章abstract：:We performed a meta-analysis to compare therapeutic outcome/safety of drug-eluting stent (DES) and conventional in-stent restenosis (ISR) treatments. We browsed through large volume of clinical data by searching MEDLINE, EMBASE, Cochrane central register of controlled trials, and EBSCO databases. In this study, 11 ran...
journal_title：Cell biochemistry and biophysics
pub_type： 杂志文章,meta分析
doi：10.1007/s12013-013-9692-7
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abstract：:The aim of the study was to evaluate the clinical efficacy of hydrogen sulfide (H2S) treatment on the endothelin-induced cardiac hypertrophy. Sixty-four adult male rats, weighing from 180 to 200 g, were randomly divided into four groups: ten in normal group, ten in sham group, 44 in model group established by inducing...
journal_title：Cell biochemistry and biophysics
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doi：10.1007/s12013-014-0113-3
更新日期：2014-12-01 00:00:00
abstract：:To explore therapeutic effects and underlying mechanism of Salubrinal combined with Ulinastatin (UTI) on acute Paraquat (PQ) poisoning. Four hundred rats were randomly allocated into UTI group, SAL group, SAL + UTI and control group according to random number table with 100 rats in each group. Acute PQ poisoning model...
journal_title：Cell biochemistry and biophysics
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doi：10.1007/s12013-014-0095-1
更新日期：2014-12-01 00:00:00
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journal_title：Cell biochemistry and biophysics
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doi：10.1007/s12013-014-9970-z
更新日期：2014-09-01 00:00:00
abstract：:The divergence of protein (Hedgehog) in different organisms remains an unresolved issue to comprehend how the pathway in Hh signaling evolves. Insights into this question can help one identify the key molecules in Hh signaling. This work proposes a protein-associated factor in cells. The development of a non-reduction...
journal_title：Cell biochemistry and biophysics
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更新日期：2014-04-01 00:00:00
abstract：:Insulin, the major secreted product of the beta-cells of the islets of Langerhans, is initially synthesized as a precursor (preproinsulin), from which the mature hormone is excised by a series of proteolytic cleavages. This review provides a personal narrative of some of the key research projects leading to the identi...
journal_title：Cell biochemistry and biophysics
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journal_title：Cell biochemistry and biophysics
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journal_title：Cell biochemistry and biophysics
pub_type： 杂志文章,评审
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journal_title：Cell biochemistry and biophysics
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更新日期：2006-01-01 00:00:00
abstract：:Ezrin is overexpressed in a variety of neoplastic cells and is involved in the later stages of tumor progression and metastasis. The present study investigated the expression and functional significance of ezrin in human brain astrocytoma. Ezrin expression was examined in specimens from healthy human brains (10 autops...
journal_title：Cell biochemistry and biophysics
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更新日期：2013-01-01 00:00:00
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更新日期：2012-07-01 00:00:00
abstract：:Cotton bollworm, Helicoverpa armigera, is one of the most damaging polyphagous pests worldwide, which has developed high levels of resistance to commonly applied insecticides. Mitochondrial P-glycoprotein (Pgp) was detected in the insecticide-resistant strain of H. armigera using C219 antibodies, and its possible role...
journal_title：Cell biochemistry and biophysics
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journal_title：Cell biochemistry and biophysics
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doi：10.1007/s12013-013-9543-6
更新日期：2013-11-01 00:00:00
abstract：:This study explored the effect of a first aid model employing two nurses on the efficient rescue operation time and the efficient resuscitation time for major burn patients. A two-nurse model of first aid was designed for major burn patients. The model includes a division of labor between the first aid nurses and the ...
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journal_title：Cell biochemistry and biophysics
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abstract：:The aim of this study was to evaluate the effect of tumor necrosis factor-alpha (TNF-α) inhibitor-infliximab on ankylosing spondylitis (AS) patients and detect the serum level of HLA-B27 and PDCD-1 before and after TNF inhibitor treatment. 138 patients at active stage of AS were treated with infliximab; serum was coll...
journal_title：Cell biochemistry and biophysics
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journal_title：Cell biochemistry and biophysics
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journal_title：Cell biochemistry and biophysics
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journal_title：Cell biochemistry and biophysics
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abstract：:MicroRNAs (miRs) play important roles in initiation and progression of many pathologic processes. However, the roles of miRs in diabetic nephropathy remain unclear. This study was to determine whether miR-21 was involved in diabetic nephropathy and to explore the relationship between miR-21 and MMP9/TIMP1 expression i...
journal_title：Cell biochemistry and biophysics
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journal_title：Cell biochemistry and biophysics
pub_type： 杂志文章,评审
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journal_title：Cell biochemistry and biophysics
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更新日期：2016-09-01 00:00:00
abstract：:A quality control method of highly diluted and potentized homeopathic remedies is important for curing patients applying homeopathic therapy. Lenger detected photons in highly potentized homeopathic remedies by delayed luminescence. The photons of Argentum metallicum 100MK and Cantharis 100MK magnetically bound to the...
journal_title：Cell biochemistry and biophysics
pub_type： 杂志文章
doi：10.1007/s12013-013-9712-7
更新日期：2014-03-01 00:00:00
abstract：:The objective is to explore the clinical curative effects of methylprednisolone combined with MTX and DXM intrathecal injection in treating neuropsychiatric systemic lupus erythematosus (NPSLE) and its effects on autoantibody level and anti-N-methyl-D-aspartate receptor subtype NR2a/2b antibody (anti-NR2 antibody) lev...
journal_title：Cell biochemistry and biophysics
pub_type： 杂志文章
doi：10.1007/s12013-014-0010-9
更新日期：2014-11-01 00:00:00
