Abstract:
:The aim of this study was to evaluate the effect of tumor necrosis factor-alpha (TNF-α) inhibitor-infliximab on ankylosing spondylitis (AS) patients and detect the serum level of HLA-B27 and PDCD-1 before and after TNF inhibitor treatment. 138 patients at active stage of AS were treated with infliximab; serum was collected before and after TNF-α inhibitor treatment for analysis. Reverse transcription-polymerase chain reaction (RT-PCR), flow cytometry, and enzyme-linked immuno sorbent assay were applied to detect the levels of HLA-B27 and PDCD-1 at different time points, which were used for statistical analysis with clinical data including two AS indicators (erythrocyte sedimentation rate--ESR and C-reactive protein--CRP). After the treatment for 6 weeks, RT-PCR showed that the gene expressions of HLA-B27 and PDCD-1 were significantly downregulated compared with baseline before infliximab treatment (P < 0.05); flow cytometry showed that the HLA-B27 and PDCD-1 double-labeled cells were significantly downregulated (P < 0.05). After 2, 6, or 10 weeks of infliximab treatment, the levels of ESR, CRP, serum HLA-B27, and PDCD-1 of the AS patients were all significantly lower than the baseline levels (P < 0.05), and the serum HLA-B27 and PDCD-1 levels were all significantly correlated with ESR (P < 0.05). Infliximab, an anti-TNF-α inhibitor, decreases significantly not only ESR and CRP, but also the serum levels of HLA-B27 and PDCD-1 in patients with AS. HLA-B27 and PDCD-1 are involved in the pathogenesis, and disease activities of AS. HLA-B27 and PDCD-1 are potentially the useful markers of AS activity and useful parameters to evaluate the effectiveness of anti-TNF-α inhibitor in treating AS.
journal_name
Cell Biochem Biophysjournal_title
Cell biochemistry and biophysicsauthors
Chen X,Zhou X,Li X,Tang J,Hu X,Wang J,Xu Cdoi
10.1007/s12013-014-0082-6subject
Has Abstractpub_date
2014-11-01 00:00:00pages
1453-7issue
2eissn
1085-9195issn
1559-0283journal_volume
70pub_type
杂志文章abstract::The aim of this study was to analyze the relationship between oolong tea drinking and bone mineral density in postmenopausal Han Chinese women, while living and diet habits, fertility, disease elements and other baseline conditions were controlled. One group included 124 cases who routinely drank oolong tea, and the o...
journal_title:Cell biochemistry and biophysics
pub_type: 杂志文章,收录出版
doi:10.1007/s12013-014-0053-y
更新日期:2014-11-01 00:00:00
abstract::Characterization of the electroclinical features and evolution of childhood occipital epilepsy of Gastaut (COE-G). Seven children were retrospectively identified as having COE-G and were followed-up clinically using EEGs. Visual manifestations were the most common ictal event. Eye-associated ictal deviation was associ...
journal_title:Cell biochemistry and biophysics
pub_type: 杂志文章
doi:10.1007/s12013-013-9594-8
更新日期:2013-01-01 00:00:00
abstract::We investigated theoretically and experimentally the Ca2+-contraction coupling in rat tracheal smooth muscle. [Ca2+]i, isometric contraction and myosin light chain (MLC) phosphorylation were measured in response to 1 mM carbachol. Theoretical modeling consisted in coupling a model of Ca2+-dependent MLC kinase (MLCK) a...
journal_title:Cell biochemistry and biophysics
pub_type: 杂志文章
doi:10.1385/CBB:46:3:233
更新日期:2006-01-01 00:00:00
abstract::A number of case-control studies have been conducted to investigate the relationship between the ATP-binding cassette transporter A1 (ABCA1) gene polymorphisms and risk of coronary heart disease (CHD). However, the results have been inconclusive. The purpose of the present study is to investigate whether this polymorp...
journal_title:Cell biochemistry and biophysics
pub_type: 杂志文章,meta分析
doi:10.1007/s12013-014-0161-8
更新日期:2015-01-01 00:00:00
abstract::Apoptosis of endothelial cells related to homocysteine (Hcy) has been reported in several studies. In this study, we evaluated whether reactive oxygen species (ROS)-producing signaling pathways contribute to Hcy-induced apoptosis induction, with specific emphasis on NADPH oxidases. Human umbilical vein endothelial cel...
journal_title:Cell biochemistry and biophysics
pub_type: 杂志文章
doi:10.1007/s12013-011-9297-y
更新日期:2013-11-01 00:00:00
abstract::Anions enter from the cytoplasm into the channel pore of the cystic fibrosis transmembrane conductance regulator (CFTR) Cl- channel not via a central pathway but via a single lateral portal or fenestration. High Cl- conductance is dependent on electrostatic attraction of cytoplasmic Cl- ions by four positively charged...
journal_title:Cell biochemistry and biophysics
pub_type: 杂志文章
doi:10.1007/s12013-019-00899-w
更新日期:2020-03-01 00:00:00
abstract::The present study aimed at detecting DNA damage and fragmentation as well as histone acetylation depending on oxidative stress caused by CCl4 intoxication. Also, the protective role of N-acetyl cysteine, a precursor for GSH, in DNA damage is investigated. Sixty rats were used in this study. In order to induce liver to...
journal_title:Cell biochemistry and biophysics
pub_type: 杂志文章
doi:10.1007/s12013-014-0031-4
更新日期:2014-11-01 00:00:00
abstract::Semaphorin4D (Sema4D) belongs to Semaphorins family and is secreted and membrane-bound protein. Its function on angiogenesis and axon regeneration makes it an ideal therapeutic target for spinal cord injury (SCI). Here we examined Sema4D expression profile by real-time PCR and western blot and found Sema4D was upregul...
journal_title:Cell biochemistry and biophysics
pub_type: 杂志文章
doi:10.1007/s12013-013-9727-0
更新日期:2014-04-01 00:00:00
abstract::Epidemiologic and experimental studies suggest that dietary fatty acids influence the development and progression of breast cancer. However, no clear data are present in literature that could demonstrate how n - 3 PUFA can interfere with breast cancer growth. It is suggested that these fatty acids might change the str...
journal_title:Cell biochemistry and biophysics
pub_type: 杂志文章
doi:10.1007/s12013-012-9365-y
更新日期:2012-09-01 00:00:00
abstract::3-Iodothyronamine (T1AM) is regarded as a hormone-like substance thanks to its endogenous nature, its interaction with specific receptors trace amine-associated receptor 1 and its biological effects. We characterized T1AM transport and conversion in an in vitro culture of H9c2 murine cells, after a T1AM bolus injectio...
journal_title:Cell biochemistry and biophysics
pub_type: 杂志文章
doi:10.1007/s12013-013-9680-y
更新日期:2014-01-01 00:00:00
abstract::The epithelial Na/Pi cotransporter (NaPi-II) is instrumental in maintaining phosphate (Pi) homeostasis in vertebrates. Hormones and metabolic factors (PTH, Pi availability) that acutely influence renal Pi excretion have been demonstrated to target NaPi-II expression. Upon stimulation, newly synthesized transporter mol...
journal_title:Cell biochemistry and biophysics
pub_type: 杂志文章,评审
doi:10.1385/CBB:36:2-3:241
更新日期:2002-01-01 00:00:00
abstract::Olfactory ensheathing cells (OECs) are of great interest for regenerative purposes since they are believed to aid axonal growth. With the view set on the strategies to achieve reconnection between neuronal structures, it is of great importance to characterize the behaviour of these cells on long thread-like structures...
journal_title:Cell biochemistry and biophysics
pub_type: 杂志文章
doi:10.1007/s12013-012-9399-1
更新日期:2013-01-01 00:00:00
abstract::The objective of this study is to observe the adult growth hormone level in postoperative pituitary tumor patients of multi-centers, and explore the change of hypophyseal hormones in postoperative pituitary tumor patients. Sixty patients with pituitary tumor admitted during March, 2011-March, 2012 were selected. Posto...
journal_title:Cell biochemistry and biophysics
pub_type: 临床试验,杂志文章,多中心研究
doi:10.1007/s12013-014-0334-5
更新日期:2015-03-01 00:00:00
abstract::Although As2O3 (ATO) has been recommended as the front-line agent for treatment of acute promyelocytic leukemia (APL), particularly for relapsed or refractory APL, it has been associated with profound toxicity. Icariin is a natural compound with activity against a variety of cancers. This study was designed to investi...
journal_title:Cell biochemistry and biophysics
pub_type: 杂志文章
doi:10.1007/s12013-015-0660-2
更新日期:2015-09-01 00:00:00
abstract::Mutations of the retinoblastoma tumor suppressor, pRb, or its cyclin-cyclin-dependent kinase (CDK) regulatory kinases or CDK inhibitors, allows unrestrained E2F activity, leading to unregulated cell cycle progression. However, overexpression of E2F-1 also sensitizes cells to apoptosis, suggesting that targeting this p...
journal_title:Cell biochemistry and biophysics
pub_type: 杂志文章
doi:10.1385/CBB:33:2:199
更新日期:2000-01-01 00:00:00
abstract::The objective of this study is to investigate the roles of acteoside (ACT) in cells with oxygen-glucose deprivation and reoxygenation (OGD/R)-induced injury and the underlying mechanisms. The differentially expressed genes (DEGs) in rats with middle cerebral artery occlusion were identified using GSE61616 data set. Ky...
journal_title:Cell biochemistry and biophysics
pub_type: 杂志文章
doi:10.1007/s12013-020-00965-8
更新日期:2021-01-13 00:00:00
abstract::Biomolecular behavior commonly involves complex sets of interacting components that are challenging to understand through solution-based chemical theories. Molecular assembly is especially intriguing in the cellular environment because of its links to cell structure in processes such as chemotaxis. We use a coarse-gra...
journal_title:Cell biochemistry and biophysics
pub_type: 杂志文章
doi:10.1385/cbb:45:2:195
更新日期:2006-01-01 00:00:00
abstract::The airways in asthma and COPD are characterized by an increase in airway smooth muscle (ASM) mass and bronchial vascular changes associated with increased expression of pro-angiogenic growth factors, such as fibroblast growth factors (FGF-1 and FGF-2) and vascular endothelial growth factor (VEGF). We investigated the...
journal_title:Cell biochemistry and biophysics
pub_type: 杂志文章
doi:10.1007/s12013-011-9331-0
更新日期:2013-11-01 00:00:00
abstract::Filamin (FLN) plays an important role in differentiation, migration, and signal transduction in vascular smooth muscle cells (VSMCs). We hypothesized that suppression of FLN expression would inhibit differentiation and migration of VSMCs, and interrupt the phenotype switch of these cells. We designed and tested differ...
journal_title:Cell biochemistry and biophysics
pub_type: 杂志文章
doi:10.1007/s12013-011-9159-7
更新日期:2011-09-01 00:00:00
abstract::Previous studies have shown that A. lumbricoides extracts capture sialic acid (SA) from human red blood cells (RBC). The aim of this work was to study hemorheological alterations in vitro caused by parasite larvae. The biorheological action of three larva concentrates of first and second larval stage on group O erythr...
journal_title:Cell biochemistry and biophysics
pub_type: 杂志文章
doi:10.1007/s12013-012-9425-3
更新日期:2013-03-01 00:00:00
abstract::cDNAs encoding two PDE-3 or cyclic GMP-inhibited (cGI) cyclic nucleotide phosphodiesterase (PDE) isoforms, RPDE-3B (RcGIP1) and HPDE-3A (HcGIP2), were cloned from rat (R) adipose tissue and human (H) heart cDNA libraries. Deletion and N- and C-terminal truncation mutants were expressed in Escherichia coli in order to ...
journal_title:Cell biochemistry and biophysics
pub_type: 杂志文章
doi:10.1007/BF02737830
更新日期:1998-01-01 00:00:00
abstract::Ezrin is overexpressed in a variety of neoplastic cells and is involved in the later stages of tumor progression and metastasis. The present study investigated the expression and functional significance of ezrin in human brain astrocytoma. Ezrin expression was examined in specimens from healthy human brains (10 autops...
journal_title:Cell biochemistry and biophysics
pub_type: 杂志文章
doi:10.1007/s12013-013-9653-1
更新日期:2013-01-01 00:00:00
abstract::The objective of this study was to assess the clinical and histopathological relationship between pulmonary fibrosis and type 1 diabetes. We examined clinical pulmonary function parameters and transbronchial lung biopsies to assess associated histopathological changes in 12 type 1 diabetic patients presenting with dys...
journal_title:Cell biochemistry and biophysics
pub_type: 杂志文章
doi:10.1007/s12013-014-0068-4
更新日期:2014-11-01 00:00:00
abstract::Osteoclast is the only cell that can degrade bone tissue in vivo. Recent studies have shown the important role of cytoskeleton dynamics in osteolysis and the formation of podosome belt in osteoclasts. This process is regulated by the dynamic microtubule (MT) network. We treated osteoclast precursor cells Raw264.7 with...
journal_title:Cell biochemistry and biophysics
pub_type: 杂志文章
doi:10.1007/s12013-014-0258-0
更新日期:2015-03-01 00:00:00
abstract::This study aims to introduce a new surgical procedure for the treatment of Arnold-Chiari type I malformation (ACM-1) and to compare its effectiveness with the techniques described in previous studies. We performed the following procedures: foramen magnum decompression combined with the removal of both the atlanto-occi...
journal_title:Cell biochemistry and biophysics
pub_type: 杂志文章
doi:10.1007/s12013-014-0135-x
更新日期:2014-12-01 00:00:00
abstract::Dendritic cell-specific intercellular adhesion molecule 3-grabbing nonintegrin (DC-SIGN) is a cellular receptor for hepatitis C virus for the binding of viral envelope glycoprotein E2. Interaction of DC-SIGN with the E2 may evoke cellular signal transduction implicated in viral pathogenesis. We developed a cell model ...
journal_title:Cell biochemistry and biophysics
pub_type: 杂志文章
doi:10.1007/s12013-009-9069-0
更新日期:2010-01-01 00:00:00
abstract::A sensitive and reliable ultra-pressure liquid chromatography with tandem mass spectrometry (UPLC-MS) was developed and validated for simultaneous quantification of six main bioactive components, i.e., calycosin-7-O-β-D-glucoside, ononin, calycosin, formononetin, astragaloside IV, and astragaloside II in rat plasma af...
journal_title:Cell biochemistry and biophysics
pub_type: 杂志文章
doi:10.1007/s12013-014-9972-x
更新日期:2014-09-01 00:00:00
abstract::Behcet's disease (BD) accompanied by intestinal involvement is called intestinal BD. Although recent studies have attained positive feedback with the administration of anti-TNF-α agents in patients with BD, only a few reports on the study of etanercept in intestinal BD have been found. In this study, 35 cases of intes...
journal_title:Cell biochemistry and biophysics
pub_type: 杂志文章
doi:10.1007/s12013-014-9860-4
更新日期:2014-07-01 00:00:00
abstract::Molecular dynamics simulations, binding free energy calculations, principle component analysis (PCA), and residue interaction network analysis were employed in order to investigate the molecular mechanism of M184I single mutation which played pivotal role in making the HIV-1 reverse transcriptase (RT) totally resistan...
journal_title:Cell biochemistry and biophysics
pub_type: 杂志文章
doi:10.1007/s12013-015-0709-2
更新日期:2016-03-01 00:00:00
abstract::The increasing use of wireless communication devices has raised major concerns towards deleterious effects of microwave radiation on human health. The aim of the study was to demonstrate the effect of low-intensity microwave radiation on levels of monoamine neurotransmitters and gene expression of their key regulating...
journal_title:Cell biochemistry and biophysics
pub_type: 杂志文章
doi:10.1007/s12013-015-0576-x
更新日期:2015-09-01 00:00:00