Inhibition of hepatitis B virus cccDNA by siRNA in transgenic mice.

Abstract:

:The elimination of viral covalently closed circular DNA (cccDNA) from the nucleus of infected hepatocytes is an obstacle to achieving sustained viral clearance during antiviral therapy of chronic hepatitis B virus (HBV) infection. The aim of our study was to determine whether treatment with siRNA is able to suppress viral cccDNA amplification using a HBV-transgenic mice model. The experimental results revealed that siRNAs can serve as efficient alternative anti-HBV agents, because they showed better inhibitory effect on viral replication and antigen expression in transgenic mice. More importantly, the siRNA markedly inhibited HBV cccDNA amplification.

journal_name

Cell Biochem Biophys

authors

Li G,Jiang G,Lu J,Chen S,Cui L,Jiao J,Wang Y

doi

10.1007/s12013-014-9847-1

subject

Has Abstract

pub_date

2014-07-01 00:00:00

pages

649-54

issue

3

eissn

1085-9195

issn

1559-0283

journal_volume

69

pub_type

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