Study of the mechanism of bystander effect of KDR-CDglyTK system mediated by adenovirus for the treatment of gastric cancer.

Abstract:

:We evaluated the relationship between cellular gap junction and bystander effect in gastric cancer SCG7901 cell killing by adenovirus-mediated KDR-CDglyTK system. SCG7901 and HeLa cells were transfected (MOI-100) with recombinant adenovirus carrying fusion gene. Transfection efficiency/fusion gene mRNA expression were determined; intercellular communications in the presence of apigenin were measured by fluorescence recovery after photobleaching (FRAP). Transfected/non-transfected cells were mixed at 5:95/10:90% ratios, respectively, incubated for 24 h with/without apigenin, and for 72 h with GCV and 5-FU. Cell survival was determined by MTT method. The transfected SCG7901/HeLa cells expressed GFP. Fluorescence intensity in SCG7901 cells decreased after photobleaching but recovered over time. Fluorescence intensity in HeLa cells also decreased after photobleaching with no recovery. Following apigenin treatment, fluorescence intensity differed between SCG7901 and HeLa cells. Survival rates of SCG7901 cells differed among prodrug, apigenin, and prodrug + apigenin groups compared with controls, but no significant difference was observed for HeLa cells. We concluded that the intercellular communication in SCG7901 cells related with the gap junctions, while there was no such communication in HeLa cells. The bystander killing effect related with the intercellular gap junctions and was increased by apigenin with no such effect seen in HeLa cells.

journal_name

Cell Biochem Biophys

authors

Qiang L,Yanping L,Zonghai H,Fei C,Zhou L,Jinlong Y

doi

10.1007/s12013-013-9599-3

subject

Has Abstract

pub_date

2013-01-01 00:00:00

pages

1021-7

issue

3

eissn

1085-9195

issn

1559-0283

journal_volume

67

pub_type

杂志文章
  • Simultaneous Determination of Human Erythrocyte Deformability and Adhesion Energy: A Novel Approach Using a Microfluidic Chamber and the "Glass Effect".

    abstract::The simultaneous determination of adhesion and deformability parameters of erythrocytes was carried out through a microfluidic device, which uses an inverted optical microscope with new image acquisition and analysis technologies. Also, an update of the models describing erythrocyte adhesion and deformation was propos...

    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章

    doi:10.1007/s12013-020-00956-9

    authors: Londero CM,Riquelme BD

    更新日期:2020-11-07 00:00:00

  • Icaritin Inhibits Skin Fibrosis through Regulating AMPK and Wnt/β-catenin Signaling.

    abstract::Skin fibrosis is one of the major features of scleroderma. WNT/β-catenin signaling is associated with the progression of skin fibrosis. In this study, we aimed to determine the effect of icaritin (IT), a natural compound, on scleroderma-related skin fibrosis and its mechanisms. We found that IT could reduce the expres...

    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章

    doi:10.1007/s12013-020-00952-z

    authors: Li M,Liu Q,He S,Kong X,Lin J,Huang Y,Wu W,Wu J

    更新日期:2020-10-30 00:00:00

  • Therapeutical effects and mechanism of salubrinal combined with ulinastatin on treating paraquat poisoning.

    abstract::To explore therapeutic effects and underlying mechanism of Salubrinal combined with Ulinastatin (UTI) on acute Paraquat (PQ) poisoning. Four hundred rats were randomly allocated into UTI group, SAL group, SAL + UTI and control group according to random number table with 100 rats in each group. Acute PQ poisoning model...

    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章

    doi:10.1007/s12013-014-0095-1

    authors: Guo H,Jiang C,Sun X

    更新日期:2014-12-01 00:00:00

  • Assessment of Coronary Flow Reserve by Adenosine Stress Myocardial Perfusion Imaging in Patients with Hypertension.

    abstract::In this study, our aim was to assess the coronary flow reserve (CFR) by performing the adenosine stress (99m)Tc-MIBI single-photon computed tomographic (SPECT) myocardial perfusion imaging in patients with hypertension. 47 hypertensive patients with normal coronary angiography were divided into 2 groups, defined by th...

    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章

    doi:10.1007/s12013-015-0600-1

    authors: Fu Q,Zhang Q,Lu W,Wang Y,Huang Y,Wang Y,Wu Q,Lu C

    更新日期:2015-11-01 00:00:00

  • Human Salivary Aldehyde Dehydrogenase: Purification, Kinetic Characterization and Effect of Ethanol, Hydrogen Peroxide and Sodium Dodecyl Sulfate on the Activity of the Enzyme.

    abstract::Human salivary aldehyde dehydrogenase (hsALDH) enzyme appears to be the first line of defense in the body against exogenous toxic aldehydes. However till date much work has not been done on this important member of the ALDH family. In this study, we have purified hsALDH to homogeneity by diethylaminoethyl-cellulose (D...

    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章

    doi:10.1007/s12013-016-0742-9

    authors: Alam MF,Laskar AA,Choudhary HH,Younus H

    更新日期:2016-09-01 00:00:00

  • PKCζ-NADPH Oxidase-PKCα Dependent Kv1.5 Phosphorylation by Endothelin-1 Modulates Nav1.5-NCX1-Cav1.2 Axis in Stimulating Ca2+ Level in Caveolae of Pulmonary Artery Smooth Muscle Cells.

    abstract::Endothelin-1 (ET-1) is a potent endogenously derived vasoconstrictor, which increases pulmonary hypertension via stimulation of [Ca2+]i level in pulmonary artery smooth muscle cells (PASMCs). In this communication, we sought to investigate the mechanism by which ET-1 causes stimulation of Ca2+ concentration in caveola...

    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章

    doi:10.1007/s12013-020-00954-x

    authors: Sarkar J,Chakraborti T,Pramanik PK,Ghosh P,Mandal A,Chakraborti S

    更新日期:2020-10-23 00:00:00

  • Endothelial signaling in leukocyte transmigration.

    abstract::Leukocyte transendothelial migration is a multistep process coordinated by chemokine receptors, integrins and cell adhesion molecules. The interaction between leukocytes and endothelial cells is accompanied by bidirectional signaling in both cell types, which is initiated following formation of specialized, "docking" ...

    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章,评审

    doi:10.1385/cbb:38:3:305

    authors: Hordijk P

    更新日期:2003-01-01 00:00:00

  • Lipids Composition in Plant Membranes.

    abstract::The paper focuses on the selected plant lipid issues. Classification, nomenclature, and abundance of fatty acids was discussed. Then, classification, composition, role, and organization of lipids were displayed. The involvement of lipids in xantophyll cycle and glycerolipids synthesis (as the most abundant of all lipi...

    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章,评审

    doi:10.1007/s12013-020-00947-w

    authors: Reszczyńska E,Hanaka A

    更新日期:2020-12-01 00:00:00

  • Non-esterified fatty acids activate the AMP-activated protein kinase signaling pathway to regulate lipid metabolism in bovine hepatocytes.

    abstract::Non-esterified fatty acids (NEFAs) act as signaling molecules involved in regulating genes expression to modulate lipid metabolism. However, the regulation mechanism of NEFAs on lipid metabolism in dairy cows is unclear. The AMP-activated protein kinase (AMPK) signaling pathway plays a key role in regulating hepatic l...

    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章

    doi:10.1007/s12013-013-9629-1

    authors: Li X,Li X,Chen H,Lei L,Liu J,Guan Y,Liu Z,Zhang L,Yang W,Zhao C,Fu S,Li P,Liu G,Wang Z

    更新日期:2013-01-01 00:00:00

  • Photoreactivity of Bis-retinoid A2E Complexed with a Model Protein in Selected Model Systems.

    abstract::The bis-retinoid N-retinyl-N-retinylidene ethanolamine (A2E) is formed as a byproduct of visual cycle in retinal pigment epithelium (RPE). It contributes to golden-yellow fluorescence of the age pigment lipofuscin, which accumulates in RPE. Lipofuscin can generate a variety of reactive oxygen species (ROS) upon blue-l...

    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章

    doi:10.1007/s12013-020-00942-1

    authors: Furso J,Zadlo A,Szewczyk G,Sarna TJ

    更新日期:2020-12-01 00:00:00

  • A causal analysis of intra-abdominal hemorrhage after reduced-size liver transplantation in rat.

    abstract::In this study, we analyzed causes of abdominal hemorrhage after reduced-size liver transplantation in rat. Healthy SD rats (weight range of 260-280 g) underwent liver transplantation, the donors were female and the recipients were male rats. The recipients were, on average, by <10 g heavier than the donors. All operat...

    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章

    doi:10.1007/s12013-011-9225-1

    authors: Jing L,Li L,Jiang-Hua R,Sheng-Ning Z,Zhu L,Lai-Bang L,Jiang L

    更新日期:2011-12-01 00:00:00

  • Determination of enantiomeric impurity of levamlodipine besylate bulk drug by capillary electrophoresis using carboxymethyl-β-cyclodextrin.

    abstract::A rapid capillary electrophoresis method using carboxymethyl-β-cyclodextrin (CM-β-CD) as chiral selector was developed and validated for the enantiomeric purity determination of levamlodipine besylate bulk drug. Several parameters for were optimized for a satisfactory enantioresolution, including pH of background elec...

    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章

    doi:10.1007/s12013-014-0106-2

    authors: Qi Y,Zhang X

    更新日期:2014-12-01 00:00:00

  • Extremely high frequency electromagnetic radiation enforces bacterial effects of inhibitors and antibiotics.

    abstract::The coherent electromagnetic radiation (EMR) of the frequency of 51.8 and 53 GHz with low intensity (the power flux density of 0.06 mW/cm(2)) affected the growth of Escherichia coli K12(lambda) under fermentation conditions: the lowering of the growth specific rate was considerably (approximately 2-fold) increased wit...

    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章

    doi:10.1007/s12013-008-9020-9

    authors: Tadevosyan H,Kalantaryan V,Trchounian A

    更新日期:2008-01-01 00:00:00

  • MT2A Promotes Oxaliplatin Resistance in Colorectal Cancer Cells.

    abstract::We aimed to understand the molecular mechanism underlying the incidence of Oxaliplatin resistance in colorectal cancer. The Oxaliplatin-resistant (OR) HT29 colorectal cell line was established by long-term exposure to Oxaliplatin. Cell viability and proliferation were determined by the 3-(4,5-dimethyl thiazol-2-yl)-2,...

    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章

    doi:10.1007/s12013-020-00930-5

    authors: Zhao Z,Zhang G,Li W

    更新日期:2020-12-01 00:00:00

  • Modulation of ion channel function by P2Y receptors.

    abstract::P2Y receptors are classified as P2 purinergic receptors that belong to the superfamily of G-protein coupled receptors. They are distinguishable from P1 (adenosine) receptors in that they bind adenine and/or uracil nucleotide triphosphates or diphosphates depending on the subtype. Over the past decade, P2Y receptors ha...

    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章,评审

    doi:10.1385/CBB:39:1:75

    authors: Lee SY,O'Grady SM

    更新日期:2003-01-01 00:00:00

  • Transarterial Chemoembolization of Giant Liver Haemangioma: A Multi-center Study with 836 Cases.

    abstract::The purpose of the study is to evaluate the effectiveness and safety of transarterial chemoembolization (TACE) with pingyangmycin-lipiodol emulsion for the treatment of symptomatic giant hepatic haemangioma. Four hospitals (The Second Hospital of Shandong University, Qilu Hospital of Shandong University, Shandong Prov...

    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章,多中心研究

    doi:10.1007/s12013-015-0680-y

    authors: Li Y,Jia Y,Li S,Wang W,Wang Z,Wang Y,Liu B,Wang W,Chang H,Li Z

    更新日期:2015-11-01 00:00:00

  • Intracellular mechanics and activity of breast cancer cells correlate with metastatic potential.

    abstract::Mechanics of cancer cells are directly linked to their metastatic potential, or ability to produce a secondary tumor at a distant site. Metastatic cells survive in the circulatory system in a non-adherent state, and can squeeze through barriers in the body. Such considerable structural changes in cells rely on rapid r...

    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章

    doi:10.1007/s12013-012-9356-z

    authors: Gal N,Weihs D

    更新日期:2012-07-01 00:00:00

  • Expression and purification of rotavirus proteins NSP5 and NSP6 in Escherichia coli.

    abstract::Rotaviruses are one of the worldwide leading causes of gastroenteritis in children under 5 yr old. The rotavirus nonstructural NSP5 is a phosphoprotein implicated in viroplasms formation, whereas NSP6 could have a possible regulatory role of NSP5. It has been reported that N- and C-termini of NSP5 are important for th...

    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章

    doi:10.1385/CBB:44:3:336

    authors: Samaniego-Hernández M,De León-Rodriguez A,Aparicio-Fabre R,Arias-Ortiz C,Barba de la Rosa AP

    更新日期:2006-01-01 00:00:00

  • Phenotype switch of vascular smooth muscle cells after siRNA silencing of filamin.

    abstract::Filamin (FLN) plays an important role in differentiation, migration, and signal transduction in vascular smooth muscle cells (VSMCs). We hypothesized that suppression of FLN expression would inhibit differentiation and migration of VSMCs, and interrupt the phenotype switch of these cells. We designed and tested differ...

    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章

    doi:10.1007/s12013-011-9159-7

    authors: Zhu G,Chen H,Zhang W

    更新日期:2011-09-01 00:00:00

  • Single Active Site Mutation Causes Serious Resistance of HIV Reverse Transcriptase to Lamivudine: Insight from Multiple Molecular Dynamics Simulations.

    abstract::Molecular dynamics simulations, binding free energy calculations, principle component analysis (PCA), and residue interaction network analysis were employed in order to investigate the molecular mechanism of M184I single mutation which played pivotal role in making the HIV-1 reverse transcriptase (RT) totally resistan...

    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章

    doi:10.1007/s12013-015-0709-2

    authors: Moonsamy S,Bhakat S,Walker RC,Soliman ME

    更新日期:2016-03-01 00:00:00

  • Intrinsically semi-disordered state and its role in induced folding and protein aggregation.

    abstract::Intrinsically disordered proteins (IDPs) refer to those proteins without fixed three-dimensional structures under physiological conditions. Although experiments suggest that the conformations of IDPs can vary from random coils, semi-compact globules, to compact globules with different contents of secondary structures,...

    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章

    doi:10.1007/s12013-013-9638-0

    authors: Zhang T,Faraggi E,Li Z,Zhou Y

    更新日期:2013-01-01 00:00:00

  • Molecular mechanisms by which saturated fatty acids modulate TNF-α expression in mouse macrophage lineage.

    abstract::Many macrophage functions are modulated by fatty acids (FAs), including cytokine release, such as tumor necrosis factor-α (TNF-α). TNF-α is of great interest due to its role in the inflammation process observed in several diseases such as rheumatoid arthritis, atherosclerosis, and obesity. However, the mechanisms by w...

    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章

    doi:10.1007/s12013-010-9117-9

    authors: de Lima-Salgado TM,Alba-Loureiro TC,do Nascimento CS,Nunes MT,Curi R

    更新日期:2011-03-01 00:00:00

  • Identification and characterization of a proteolysis-resistant fragment containing the PCI domain in the Arabidopsis thaliana INT6/eIF3e translation factor.

    abstract::The PCI domain comprises approx 200 amino acids and is found in subunits of the eukaryotic translation initiation factor 3 (eIF3), the 26S proteasome and the COP9/signalosome complexes. The PCI domain is involved in protein-protein interaction, and mouse INT6 truncated proteins lacking the PCI domain show cell maligna...

    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章

    doi:10.1385/CBB:44:3:522

    authors: Murai MJ,Carneiro FR,Gozzo FC,Ierardi DF,Pertinhez TA,Zanchin NI

    更新日期:2006-01-01 00:00:00

  • (Pro)Insulin processing: a historical perspective.

    abstract::Insulin, the major secreted product of the beta-cells of the islets of Langerhans, is initially synthesized as a precursor (preproinsulin), from which the mature hormone is excised by a series of proteolytic cleavages. This review provides a personal narrative of some of the key research projects leading to the identi...

    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章,评审

    doi:10.1385/cbb:40:3:143

    authors: Davidson HW

    更新日期:2004-01-01 00:00:00

  • Sorting of peroxisomal and mitochondrial carnitine acetyltransferase isozymes in the diploid yeast, Candida tropicalis.

    abstract::Peroxisomal and mitochondrial carnitine acetyltransferase (CAT; EC 2.3.1.7) isozymes are synthesized from the first and second ATG codons of the open reading frame of one gene, Candida tropicalis CAT. Primer extension analysis and RNase protection assay revealed that the peroxisomal CAT, initiating at the second AUG c...

    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章

    doi:10.1385/cbb:32:1-3:139

    authors: Tanaka A,Ueda M

    更新日期:2000-01-01 00:00:00

  • Advantages, Disadvantages, and Trend of Integrative Medicine in the Treatment of Heart Failure.

    abstract::Integrative medicine therapy using traditional Chinese medicine (TCM) combined with western medicine has shown some advantages in treating heart failure (HF), such as holistic concept; multi-target treatment; dialectical logic; personalized therapy; formulae compatibility; and reduction of side effects of western medi...

    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章,评审

    doi:10.1007/s12013-014-0466-7

    authors: Zhang P

    更新日期:2015-06-01 00:00:00

  • Impact analysis of autoantibody level and NR2 antibody level in neuropsychiatric SLE treated by methylprednisolone combined with MTX and DXM intrathecal injection.

    abstract::The objective is to explore the clinical curative effects of methylprednisolone combined with MTX and DXM intrathecal injection in treating neuropsychiatric systemic lupus erythematosus (NPSLE) and its effects on autoantibody level and anti-N-methyl-D-aspartate receptor subtype NR2a/2b antibody (anti-NR2 antibody) lev...

    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章

    doi:10.1007/s12013-014-0010-9

    authors: Wang J,Zhao Y,Zhang J,Lei H,Zhu G,Fu B

    更新日期:2014-11-01 00:00:00

  • Radiophotographically Controlled Nasointestinal Intubation to Treat Recurrent Postoperative Adhesive Ileus.

    abstract::This study describes clinical experience and curative effect of radiophotographically controlled nasointestinal intubation in the treatment of small intestine arrangement in recurrent postoperative adhesive ileus. A 300-cm-long nasointestinal tube was installed into the jejunum through the nasal cavity under radiophot...

    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章

    doi:10.1007/s12013-014-0496-1

    authors: Li RH,Li DC,Lv XP,Quan B

    更新日期:2015-06-01 00:00:00

  • Mechanical induction of BMP-7 in osteocyte blocks glucocorticoid-induced apoptosis through PI3K/AKT/GSK3β pathway.

    abstract::Mechanical loading is known to promote osteocyte survival, whereas glucocorticoid treatment results in osteocyte apoptosis. Here, we report that BMP-7, which was secreted by osteocyte in response to mechanical loading, exerts anti-apoptotic effect against dexamethasone-induced apoptosis of osteocytes. We further show ...

    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章

    doi:10.1007/s12013-013-9543-6

    authors: Wang Z,Guo J

    更新日期:2013-11-01 00:00:00

  • Correlations between CT perfusion parameters and vascular endothelial growth factor expression and microvessel density in implanted VX2 lung tumors.

    abstract::To evaluate the correlation between CT perfusion parameters and tumor angiogenesis, expression of VEGF is implanted in VX2 lung tumor. VX2 tumor cells were injected in 15 healthy New Zealand rabbits. After 20 days, tumors with diameter over 7 mm were scanned with 16 row spiral CT. Using Software Perfusion 3, blood flo...

    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章

    doi:10.1007/s12013-014-9966-8

    authors: Ling S,Deng D,Mo Y,Zhang X,Guan X,Wei Q

    更新日期:2014-09-01 00:00:00