Genetic evaluation of peroxisomal and cytosolic acetoacetyl-CoA thiolase isozymes in n-alkane-assimilating diploid yeast, Candida tropicalis.

Abstract:

:The n-alkane-assimilating diploid yeast, Candida tropicalis, possesses two acetoacetyl-CoA thiolase (Thiolase I) isozymes encoded by one allele: peroxisomal and cytosolic Thiolase Is encoded by both CT-T1A and CT-T1B. To clarify the function of peroxisomal and cytosolic Thiolase Is, the site-directed mutation leading Thiolase I delta C6 without a putative C-terminal peroxisomal targeting signal was introduced on CT-T1A locus in the ct-t1b delta-null mutant. The C-terminus-truncated Thiolase I was active and solely present in the cytosol. Although the ct-t1a delta/t1b delta-null mutants showed mevalonate auxotrophy, the mutants having the C-terminus-truncated Thiolase I did not require mevalonate for growth, as did the strains having cytosolic Thiolase I. These results demonstrate that the presence of Thiolase I in the cytoplasm is indispensable for the sterol synthesis in this yeast. It is of greater interest that peroxisomal and cytosolic Thiolase I isozymes, products of the same genes, play different roles in the respective compartments, although further investigations will be necessary to analyze how to be sorted into peroxisomes and the cytosol.

journal_name

Cell Biochem Biophys

authors

Ueda M,Kanayama N,Tanaka A

doi

10.1385/cbb:32:1-3:285

subject

Has Abstract

pub_date

2000-01-01 00:00:00

pages

285-90

eissn

1085-9195

issn

1559-0283

journal_volume

32 Spring

pub_type

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