Alteration in the balance of prosurvival and proapoptotic signalling pathways leads to sequence-dependent synergism between docetaxel and sorafenib in human non-small cell lung cancer cell lines.


:To examine the antiproliferative effect of the combination of docetaxel and sorafenib, applied to the representative non-small cell lung cancer cell line A549 cells either wild type or with acquired resistance to docetaxel (A549/D). The aim of this study is to evaluate the synergistic effect of combination treatment on cell growth inhibition and to elucidate the involved molecular mechanisms. A549 cells with acquired resistance to docetaxel were established by continuous exposure to docetaxel. We examined the effect of different combinatorial treatment on cell proliferation and cell cycle distribution. In addition, the effect of combinatorial treatments on proliferative and apoptotic signalling pathway were studied. Our results showed that the synergistic effect presented when A549 cells were treated with docetaxel followed by sorafenib or when A549/D cells were treated in reverse sequence. Furthermore, we suggested that synergistic effect in A549/D cells was caused by inhibiting P-gp function and altering in the balance of growth and apoptotic signalling pathways. Our data suggested a potential role of sorafenib in chemosensitizing docetaxel-resistant cancer cells. This study also provides molecular evidence for applying different therapeutic strategies for patients with different genetic and proteomic profile.


Cell Biochem Biophys


He X,Zhang T




Has Abstract


2014-03-01 00:00:00












  • The -319C/+49G/CT60G haplotype of CTLA-4 gene confers susceptibility to rheumatoid arthritis in Mexican population.

    abstract::Several single nucleotide polymorphisms (SNPs) within the CTLA-4 gene and elevated serum levels of soluble CTLA-4 (sCTLA-4) have been associated with autoimmunity including rheumatoid arthritis (RA). In this case-control study, we evaluated the relationship between the -319C/T (rs5742909) and CT60 G/A (rs3087243) SNPs...

    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章


    authors: Torres-Carrillo N,Ontiveros-Mercado H,Torres-Carrillo NM,Parra-Rojas I,Rangel-Villalobos H,Ramírez-Dueñas MG,Gutiérrez-Ureña SR,Valle Y,Muñoz-Valle JF

    更新日期:2013-01-01 00:00:00

  • Functions and roles of a protein-associated factor.

    abstract::The divergence of protein (Hedgehog) in different organisms remains an unresolved issue to comprehend how the pathway in Hh signaling evolves. Insights into this question can help one identify the key molecules in Hh signaling. This work proposes a protein-associated factor in cells. The development of a non-reduction...

    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章


    authors: Sarker MM,Zhou MC,Rameshwar P,Hanover JA

    更新日期:2014-04-01 00:00:00

  • EPR Characterization of Dinitrosyl Iron Complexes with Thiol-Containing Ligands as an Approach to Their Identification in Biological Objects: An Overview.

    abstract::The overview demonstrates how the use of only one physico-chemical approach, viz., the electron paramagnetic resonance method, allowed detection and identification of dinitrosyl iron complexes with thiol-containing ligands in various animal and bacterial cells. These complexes are formed in biological objects in the p...

    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章,评审


    authors: Vanin AF

    更新日期:2018-06-01 00:00:00

  • Molecular dynamics simulation of a high-affinity antibody-protein complex: the binding site is a mosaic of locally flexible and preorganized rigid regions.

    abstract::One nanosecond molecular dynamic (MD) simulation of anti-hen egg white lysozyme (HEL) antibody HyHEL63 (HH63) complexed with HEL reveals rigid and flexible regions of the HH63 binding site. Fifty conformations, extracted from the MD trajectory at regular time intervals were superimposed on HH63-HEL X-ray crystal struc...

    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章


    authors: Sinha N,Smith-Gill SJ

    更新日期:2005-01-01 00:00:00

  • Negative regulation of Janus kinases.

    abstract::The precise regulation of both the magnitude and the duration of Janus kinase (JAK) catalytic activity is essential for the cytokine orchestration of many biological processes, and the dysregulation of JAK activity has pathological implications. Immunosuppressive disease states, such as X-linked severe combined immuno...

    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章,评审


    authors: Duhé RJ,Wang LH,Farrar WL

    更新日期:2001-01-01 00:00:00

  • Activation of p38 MAPK pathway by hepatitis C virus E2 in cells transiently expressing DC-SIGN.

    abstract::Dendritic cell-specific intercellular adhesion molecule 3-grabbing nonintegrin (DC-SIGN) is a cellular receptor for hepatitis C virus for the binding of viral envelope glycoprotein E2. Interaction of DC-SIGN with the E2 may evoke cellular signal transduction implicated in viral pathogenesis. We developed a cell model ...

    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章


    authors: Chen QL,Zhu SY,Bian ZQ,Zhao LJ,Cao J,Pan W,Qi ZT

    更新日期:2010-01-01 00:00:00

  • Study of the mechanism of bystander effect of KDR-CDglyTK system mediated by adenovirus for the treatment of gastric cancer.

    abstract::We evaluated the relationship between cellular gap junction and bystander effect in gastric cancer SCG7901 cell killing by adenovirus-mediated KDR-CDglyTK system. SCG7901 and HeLa cells were transfected (MOI-100) with recombinant adenovirus carrying fusion gene. Transfection efficiency/fusion gene mRNA expression were...

    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章


    authors: Qiang L,Yanping L,Zonghai H,Fei C,Zhou L,Jinlong Y

    更新日期:2013-01-01 00:00:00

  • Identification and characterization of a proteolysis-resistant fragment containing the PCI domain in the Arabidopsis thaliana INT6/eIF3e translation factor.

    abstract::The PCI domain comprises approx 200 amino acids and is found in subunits of the eukaryotic translation initiation factor 3 (eIF3), the 26S proteasome and the COP9/signalosome complexes. The PCI domain is involved in protein-protein interaction, and mouse INT6 truncated proteins lacking the PCI domain show cell maligna...

    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章


    authors: Murai MJ,Carneiro FR,Gozzo FC,Ierardi DF,Pertinhez TA,Zanchin NI

    更新日期:2006-01-01 00:00:00

  • Insulin receptors and downstream substrates associate with membrane microdomains after treatment with insulin or chromium(III) picolinate.

    abstract::We have examined the association of insulin receptors (IR) and downstream signaling molecules with membrane microdomains in rat basophilic leukemia (RBL-2H3) cells following treatment with insulin or tris(2-pyridinecarbxylato)chromium(III) (Cr(pic)(3)). Single-particle tracking demonstrated that individual IR on these...

    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章


    authors: Al-Qatati A,Winter PW,Wolf-Ringwall AL,Chatterjee PB,Van Orden AK,Crans DC,Roess DA,Barisas BG

    更新日期:2012-04-01 00:00:00

  • Posterior fossa decompression combined with resection of the cerebellomedullary fissure membrane and expansile duraplasty: a radical and rational surgical treatment for Arnold-Chiari type I malformation.

    abstract::This study aims to introduce a new surgical procedure for the treatment of Arnold-Chiari type I malformation (ACM-1) and to compare its effectiveness with the techniques described in previous studies. We performed the following procedures: foramen magnum decompression combined with the removal of both the atlanto-occi...

    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章


    authors: Liang CJ,Dong QJ,Xing YH,Shan M,Wen LX,Qiang ZY,Ping ZQ,Tao PZ,Ping HX

    更新日期:2014-12-01 00:00:00

  • Request for applications: islet cell replacement in type 1 diabetes.

    abstract::The American Diabetes Association announces funding for clinical and basic science studies in three major areas of research that focus on cell-based insulin replacement in type 1 diabetes: 1. Genetic engineering of nonpancreatic cells into glucose-sensitive insulin-producing cells; 2. Transforming stem cells, pancreat...

    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章


    authors: Campbell S

    更新日期:2004-01-01 00:00:00

  • Evaluation of global longitudinal strain of left ventricle and regional longitudinal strain in the region of left ventricular leads predicts the response to cardiac resynchronization therapy in patients with ischemic heart failure.

    abstract::Myocardium viability in ischemic heart failure (HF) may affect the effect of cardiac resynchronization therapy (CRT). We hypothesized that longitudinal strain of 2D-STE, which reflects myocardium viability, can predict the response to CRT in patients with ischemic HF. 2D-STE was performed in 42 patients with HF, 1 wee...

    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章


    authors: Ma CY,Liu S,Yang J,Tang L,Zhang LM,Li N,Yu B

    更新日期:2014-09-01 00:00:00

  • Chromosome analysis using spectral karyotyping (SKY).

    abstract::Spectral karyotyping is a novel technique for chromosome analysis that has been developed based on the approach of the fluorescence in situ hybridization technique. Spectral karyotyping makes it feasible to diagnose a variety of diseases, because of its technology in painting each of the 24 human chromosomes with diff...

    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章,评审


    authors: Imataka G,Arisaka O

    更新日期:2012-01-01 00:00:00

  • Modelling the role of intrinsic electric fields in microtubules as an additional control mechanism of bi-directional intracellular transport.

    abstract::Active transport is essential for cellular function, while impaired transport has been linked to diseases such as neuronal degeneration. Much long distance transport in cells uses opposite polarity molecular motors of the kinesin and dynein families to move cargos along microtubules. It is clear that many types of car...

    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章


    authors: Sataric MV,Budinski-Petkovic L,Loncarevic I,Tuszynski JA

    更新日期:2008-01-01 00:00:00

  • The Use of Calcium Channel Blockers in the Treatment of Coronary Spasm and Atrioventricular Block.

    abstract::Calcium channel blockers have been used in the treatment of coronary artery spasm for many years. However, there is insufficient knowledge about their application to treat atrioventricular block caused by coronary spasm. Clinical data of five patients who were diagnosed with coronary spasm caused by atrioventricular b...

    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章


    authors: Gao B,Zhang Z,Qian J,Cao C,Hua X,Chu M,He X,Zeng H

    更新日期:2015-06-01 00:00:00

  • Enteral ecoimmunonutrition reduced enteral permeability and serum ghrelin activity in severe cerebral stroke patients with lung infection.

    abstract::The study analyzed how enteral ecoimmunonutrition, which comprises probiotics, glutamine, fish oil, and Enteral Nutritional Suspension (TPF), can impact on the enteral permeability and serum Ghrelin activity in severe cerebral stroke patients with lung infection. Among 190 severe cerebral stroke patients with toleranc...

    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章


    authors: Xu XD,Shao F

    更新日期:2015-01-01 00:00:00

  • Ecto-mesenchymal stem cells from facial process: potential for muscle regeneration.

    abstract::Ecto-mesenchymal stem cells (EMSCs) originate from the cranial neural crest and participate in the formation of tooth, salivary, and muscle in early development stage. The transplantation of EMSCs, a potential source of myoblast stem cell, might improve muscle regeneration. The purpose of this study was to explore whe...

    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章


    authors: Nie X,Xing Y,Deng M,Gang L,Liu R,Zhang Y,Wen X

    更新日期:2014-09-01 00:00:00

  • Oolong tea drinking could help prevent bone loss in postmenopausal Han Chinese women.

    abstract::The aim of this study was to analyze the relationship between oolong tea drinking and bone mineral density in postmenopausal Han Chinese women, while living and diet habits, fertility, disease elements and other baseline conditions were controlled. One group included 124 cases who routinely drank oolong tea, and the o...

    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章,收录出版


    authors: Wang G,Liu G,Zhao H,Zhang F,Li S,Chen Y,Zhang Z

    更新日期:2014-11-01 00:00:00

  • The Role of Arachidonic Acid Metabolism in Myocardial Ischemia-Reperfusion Injury.

    abstract::Patients with myocardial ischemic diseases or who are undergoing one of various heart treatments, such as open heart surgery, coronary artery bypass grafting, percutaneous coronary artery intervention or drug thrombolysis, face myocardial ischemia-reperfusion injury (MIRI). However, no effective treatment is currently...

    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章,评审


    authors: Zhang C,He M,Ni L,He K,Su K,Deng Y,Li Y,Xia H

    更新日期:2020-09-01 00:00:00

  • Construction of small-caliber, polydioxanone cyclohexanone vascular stents.

    abstract::Objective of this article is to construct and characterize a three-layered small-caliber, artificial vascular stent. The outer layer of the stent consisted of small intestine submucosa (SIS), the middle layer was the polydioxanone (PDS) vascular stents, and the inner layer. The SIS and PDS were attached with 8-0 PDS t...

    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章


    authors: You Q,Wang F,Duan L,Du X,Xiao M,Shen Z

    更新日期:2010-05-01 00:00:00

  • Expression and purification of rotavirus proteins NSP5 and NSP6 in Escherichia coli.

    abstract::Rotaviruses are one of the worldwide leading causes of gastroenteritis in children under 5 yr old. The rotavirus nonstructural NSP5 is a phosphoprotein implicated in viroplasms formation, whereas NSP6 could have a possible regulatory role of NSP5. It has been reported that N- and C-termini of NSP5 are important for th...

    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章


    authors: Samaniego-Hernández M,De León-Rodriguez A,Aparicio-Fabre R,Arias-Ortiz C,Barba de la Rosa AP

    更新日期:2006-01-01 00:00:00

  • Impact analysis of autoantibody level and NR2 antibody level in neuropsychiatric SLE treated by methylprednisolone combined with MTX and DXM intrathecal injection.

    abstract::The objective is to explore the clinical curative effects of methylprednisolone combined with MTX and DXM intrathecal injection in treating neuropsychiatric systemic lupus erythematosus (NPSLE) and its effects on autoantibody level and anti-N-methyl-D-aspartate receptor subtype NR2a/2b antibody (anti-NR2 antibody) lev...

    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章


    authors: Wang J,Zhao Y,Zhang J,Lei H,Zhu G,Fu B

    更新日期:2014-11-01 00:00:00

  • Implementation of a fast-track clinical pathway decreases postoperative length of stay and hospital charges for liver resection.

    abstract::A fast-track clinical pathway is designed to streamline patient care delivery and maximize cost effectiveness. It has decreased postoperative length of stay (LOS) and hospital charges for many surgical procedures. However, data on clinical pathways after liver surgery are sparse. This study examined whether use of a f...

    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章


    authors: Lin DX,Li X,Ye QW,Lin F,Li LL,Zhang QY

    更新日期:2011-11-01 00:00:00

  • Electrical inhomogeneity in left ventricular hypertrophy.

    abstract::Recent studies designed to assess the relationship between aortic compliance and heterogeneity of heart electrical activity has shown that hypertrophy aggravates repolarization disturbances in the myocardium. Numerous mechanisms of electrical instability and inhomogeneity associated with left ventricular hypertrophy a...

    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章,评审


    authors: Gao C,Yang D

    更新日期:2014-07-01 00:00:00

  • Electrophoretic separation of ventricular myosin isoenzymes using a native polyacrylamide minigel system.

    abstract::A method is presented to separate rabbit cardiac ventricular myosin isoenzymes (V(1), V(2), V(3)), which are large and important contractile proteins. This polyacrylamide gel electrophoresis--using a slab minigel format--does not involve preparation of an acrylamide gradient or denaturing conditions. The isoenzyme mig...

    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章


    authors: Garcia Pomblum SC,Pomblum VJ,Gams E,Reiser PJ,Schipke JD

    更新日期:2003-01-01 00:00:00

  • Screening of Inherited Metabolic Disorders in Infants with Infantile Spasms.

    abstract::The objective of this study is to explore the incidence of inherited metabolic disorders (IMD) in infants with infantile spasms (IS), with an attempt to improve the early diagnosis and etiological and symptomatic treatment. Urine and blood samples were collected from 60 IS patients and analyzed for the quantification ...

    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章


    authors: Liu XM,Li R,Chen SZ,Sang Y,Chen J,Fan CH

    更新日期:2015-05-01 00:00:00

  • VEGFR, RET, and RAF/MEK/ERK pathway take part in the inhibition of osteosarcoma MG63 cells with sorafenib treatment.

    abstract::Osteosarcoma (OS) is the leading primary malignant bone tumor in children and young adults. It is response for a high mortality rate. Nowadays, few researches have been performed on sorafenib against OS and no tools are available to guide the use of sorafenib in the OS treatment. In this study, we aim to investigate t...

    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章


    authors: Mei J,Zhu X,Wang Z,Wang Z

    更新日期:2014-05-01 00:00:00

  • Differential effects of G- and F-actin on the plasma membrane calcium pump activity.

    abstract::We have previously shown that plasma membrane calcium ATPase (PMCA) pump activity is affected by the membrane protein concentration (Vanagas et al., Biochim Biophys Acta 1768:1641-1644, 2007). The results of this study provided evidence for the involvement of the actin cytoskeleton. In this study, we explored the rela...

    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章


    authors: Vanagas L,de La Fuente MC,Dalghi M,Ferreira-Gomes M,Rossi RC,Strehler EE,Mangialavori IC,Rossi JP

    更新日期:2013-05-01 00:00:00

  • Analysis of volatile organic compounds liberated and metabolised by human umbilical vein endothelial cells (HUVEC) in vitro.

    abstract::Gas chromatography with mass spectrometric detection combined with head-space needle trap extraction as the pre-concentration technique was applied to identify and quantify volatile organic compounds released or metabolised by human umbilical vein endothelial cells. Amongst the consumed species there were eight aldehy...

    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章


    authors: Mochalski P,Theurl M,Sponring A,Unterkofler K,Kirchmair R,Amann A

    更新日期:2015-01-01 00:00:00

  • Redox buffer capacity of the cell: theoretical and experimental approach.

    abstract::Reactive oxygen species (ROS) are involved in a variety of biological phenomena, such as mutation, carcinogenesis, inflammation, aging, development, and signal transduction. Intracellular generation of ROS might lead to the activation of redox signaling or oxidative stress. Nonetheless, it is difficult to estimate whe...

    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章


    authors: Martinovich GG,Martinovich IV,Cherenkevich SN,Sauer H

    更新日期:2010-11-01 00:00:00