Abstract：
:To examine the antiproliferative effect of the combination of docetaxel and sorafenib, applied to the representative non-small cell lung cancer cell line A549 cells either wild type or with acquired resistance to docetaxel (A549/D). The aim of this study is to evaluate the synergistic effect of combination treatment on cell growth inhibition and to elucidate the involved molecular mechanisms. A549 cells with acquired resistance to docetaxel were established by continuous exposure to docetaxel. We examined the effect of different combinatorial treatment on cell proliferation and cell cycle distribution. In addition, the effect of combinatorial treatments on proliferative and apoptotic signalling pathway were studied. Our results showed that the synergistic effect presented when A549 cells were treated with docetaxel followed by sorafenib or when A549/D cells were treated in reverse sequence. Furthermore, we suggested that synergistic effect in A549/D cells was caused by inhibiting P-gp function and altering in the balance of growth and apoptotic signalling pathways. Our data suggested a potential role of sorafenib in chemosensitizing docetaxel-resistant cancer cells. This study also provides molecular evidence for applying different therapeutic strategies for patients with different genetic and proteomic profile.
journal_name
Cell Biochem Biophysjournal_title
Cell biochemistry and biophysicsauthors
He X,Zhang Tdoi
10.1007/s12013-013-9722-5subject
Has Abstractpub_date
2014-03-01 00:00:00pages
411-8issue
2eissn
1085-9195issn
1559-0283journal_volume
68pub_type
杂志文章abstract：:Integrative medicine therapy using traditional Chinese medicine (TCM) combined with western medicine has shown some advantages in treating heart failure (HF), such as holistic concept; multi-target treatment; dialectical logic; personalized therapy; formulae compatibility; and reduction of side effects of western medi...
journal_title：Cell biochemistry and biophysics
pub_type： 杂志文章,评审
doi：10.1007/s12013-014-0466-7
更新日期：2015-06-01 00:00:00
abstract：:The PCI domain comprises approx 200 amino acids and is found in subunits of the eukaryotic translation initiation factor 3 (eIF3), the 26S proteasome and the COP9/signalosome complexes. The PCI domain is involved in protein-protein interaction, and mouse INT6 truncated proteins lacking the PCI domain show cell maligna...
journal_title：Cell biochemistry and biophysics
pub_type： 杂志文章
doi：10.1385/CBB:44:3:522
更新日期：2006-01-01 00:00:00
abstract：:In this study, the anti-proliferative effect of physcion, an anthraquinone derivative isolated and characterized from both terrestrial and marine sources, against human gastric cancer SGC-7901 cells was investigated and the underlying mechanisms were explored. Physcion reduced SGC-7901 cell viability in a dose- and ti...
journal_title：Cell biochemistry and biophysics
pub_type： 杂志文章
doi：10.1007/s12013-015-0674-9
更新日期：2015-11-01 00:00:00
abstract：:Human salivary aldehyde dehydrogenase (hsALDH) enzyme appears to be the first line of defense in the body against exogenous toxic aldehydes. However till date much work has not been done on this important member of the ALDH family. In this study, we have purified hsALDH to homogeneity by diethylaminoethyl-cellulose (D...
journal_title：Cell biochemistry and biophysics
pub_type： 杂志文章
doi：10.1007/s12013-016-0742-9
更新日期：2016-09-01 00:00:00
abstract：:Leptin is a protein hormone which plays a critical role in the regulation of both body-weight through reducing food intake and stimulating energy expenditure. Several polymorphisms in leptin gene (LEP), which encodes for leptin, have been described. However, its association with obesity is still controversial. Therefo...
journal_title：Cell biochemistry and biophysics
pub_type： 杂志文章
doi：10.1007/s12013-012-9427-1
更新日期：2013-03-01 00:00:00
abstract：:Na,K-ATPase (EC 3.6.1.37, Na,K-ATPase) is a fundamental vital membrane transport and receptor system which, after biosynthesis, is exported to the plasma membrane in inside-out vesicles. Na,K-ATPase can be extracted form the natural membrane and inserted into artificially formed phosphatidylcholine vesicles (liposomes...
journal_title：Cell biochemistry and biophysics
pub_type： 杂志文章
doi：10.1007/BF02738123
更新日期：1999-01-01 00:00:00
abstract：:Semaphorin4D (Sema4D) belongs to Semaphorins family and is secreted and membrane-bound protein. Its function on angiogenesis and axon regeneration makes it an ideal therapeutic target for spinal cord injury (SCI). Here we examined Sema4D expression profile by real-time PCR and western blot and found Sema4D was upregul...
journal_title：Cell biochemistry and biophysics
pub_type： 杂志文章
doi：10.1007/s12013-013-9727-0
更新日期：2014-04-01 00:00:00
abstract：:Elevated blood pressure is regarded as an independent risk factor for cardiovascular diseases and diabetes. We examined the relation between hematocrit and pre-hypertension as well as the effect of sex, obesity, fasting glucose, and lipids in Chinese adults. The study samples were from a community-based health examina...
journal_title：Cell biochemistry and biophysics
pub_type： 杂志文章
doi：10.1007/s12013-014-0318-5
更新日期：2015-03-01 00:00:00
abstract：:Epithelial ovarian cancer (EOC) is the second leading cause of death from gynecological malignancies worldwide. Enhancer of zeste homology 2 (EZH2), participating in gene expression silencing by trimethylating histone 3 lysine 27 (H3K27me3), is often up-regulated in EOC. ARHI, an imprinted tumor-suppressor gene, is ma...
journal_title：Cell biochemistry and biophysics
pub_type： 杂志文章
doi：10.1007/s12013-014-0168-1
更新日期：2015-01-01 00:00:00
abstract：:The increasing use of wireless communication devices has raised major concerns towards deleterious effects of microwave radiation on human health. The aim of the study was to demonstrate the effect of low-intensity microwave radiation on levels of monoamine neurotransmitters and gene expression of their key regulating...
journal_title：Cell biochemistry and biophysics
pub_type： 杂志文章
doi：10.1007/s12013-015-0576-x
更新日期：2015-09-01 00:00:00
abstract：:Resveratrol (RSV) is known for its antioxidant properties; however, this compound has been proposed to have cytotoxic and pro-oxidant effects depending on its concentration and time of exposure. We previously reported the cell cycle arrest effect of low doses of RSV in GRX cells, an activated hepatic stellate cell mod...
journal_title：Cell biochemistry and biophysics
pub_type： 杂志文章
doi：10.1007/s12013-013-9703-8
更新日期：2014-03-01 00:00:00
abstract：:The dynamic micromechanical and structural properties of single human red blood cells are studied using a combination of dual trap optical tweezers and confocal Raman spectroscopy. Such a combination permits us to show a direct relationship between the rheological properties and chemical structure conformation. The fr...
journal_title：Cell biochemistry and biophysics
pub_type： 杂志文章
doi：10.1007/s12013-012-9439-x
更新日期：2013-04-01 00:00:00
abstract：:Proper administration of antithrombotic and antiplatelet drugs after percutaneous coronary intervention (PCI) in patients with acute coronary syndrome (ACS) and renal insufficiency is a challenging task. In this study, we utilized Fondaparinux and Tirofiban (either separately or combined) to treat post-PCI patients wi...
journal_title：Cell biochemistry and biophysics
pub_type： 杂志文章,随机对照试验
doi：10.1007/s12013-015-0580-1
更新日期：2015-12-01 00:00:00
abstract：:A fast-track clinical pathway is designed to streamline patient care delivery and maximize cost effectiveness. It has decreased postoperative length of stay (LOS) and hospital charges for many surgical procedures. However, data on clinical pathways after liver surgery are sparse. This study examined whether use of a f...
journal_title：Cell biochemistry and biophysics
pub_type： 杂志文章
doi：10.1007/s12013-011-9203-7
更新日期：2011-11-01 00:00:00
abstract：:Nano-drug delivery systems have proven to be an efficient formulation tool to overcome the challenges with current antibiotics therapy and resistance. A series of pH-responsive lipid molecules were designed and synthesized for future liposomal formulation as a nano-drug delivery system for vancomycin at the infection ...
journal_title：Cell biochemistry and biophysics
pub_type： 杂志文章
doi：10.1007/s12013-017-0786-5
更新日期：2018-06-01 00:00:00
abstract：:Recent advances in the use of the atomic force microscope (AFM) for manipulating cell membranes and membrane proteins are reviewed. Early pioneering work on measurements of the magnitude of the force required to create indentations with defined depth on their surfaces and to separate interacting pairs of avidin-biotin...
journal_title：Cell biochemistry and biophysics
pub_type： 杂志文章,评审
doi：10.1385/CBB:39:3:257
更新日期：2003-01-01 00:00:00
abstract：:The aim of this study is to examine the efficacy of alprostadil liposomal preparation in the treatment of blue toe syndrome. As many as 32 patients with blue toe syndrome were randomized into the test group and a control group. Patients out of the test group were treated with alprostadil liposomal preparation, while t...
journal_title：Cell biochemistry and biophysics
pub_type： 杂志文章,随机对照试验
doi：10.1007/s12013-014-0448-9
更新日期：2015-05-01 00:00:00
abstract：:The aim of this study was to analyze the relationship between oolong tea drinking and bone mineral density in postmenopausal Han Chinese women, while living and diet habits, fertility, disease elements and other baseline conditions were controlled. One group included 124 cases who routinely drank oolong tea, and the o...
journal_title：Cell biochemistry and biophysics
pub_type： 杂志文章,收录出版
doi：10.1007/s12013-014-0053-y
更新日期：2014-11-01 00:00:00
abstract：:Many of the structural domains involved in Ca2+ channel (CACN) inactivation are also involved in determining their sensitivity to antagonist inhibition. We hypothesize that differences in inactivation properties and their structural determinants may suggest candidate domains as targets for the development of novel, se...
journal_title：Cell biochemistry and biophysics
pub_type： 杂志文章
doi：10.1385/CBB:45:3:229
更新日期：2006-01-01 00:00:00
abstract：:The remarkable ability of living cells to sense, process, and respond to mechanical stimuli in their environment depends on the rapid and efficient interconversion of mechanical and chemical energy at specific times and places within the cell. For example, application of force to cells leads to conformational changes ...
journal_title：Cell biochemistry and biophysics
pub_type： 杂志文章,评审
doi：10.1007/s12013-007-0013-x
更新日期：2007-01-01 00:00:00
abstract：:Diabetic cardiomyopathy is a leading cause of morbidity and mortality, and Insulin2 mutant (Ins2+/-) Akita is a genetic mice model of diabetes relevant to humans. Dicer, miRNAs, and inflammatory cytokines are associated with heart failure. However, the differential expression of miRNAs, dicer, and inflammatory molecul...
journal_title：Cell biochemistry and biophysics
pub_type： 杂志文章
doi：10.1007/s12013-013-9679-4
更新日期：2014-01-01 00:00:00
abstract：:Gastrointestinal stromal tumors (GISTs) are defined as spindle cell and/or epithelioid tumors originated from interstitial Cajal cells or precursors in the digestive tract. This study was conducted to identify genes differing in expression between the gastric tumors and the adjacent non-cancerous mucosas in patients w...
journal_title：Cell biochemistry and biophysics
pub_type： 杂志文章
doi：10.1007/s12013-014-0504-5
更新日期：2015-06-01 00:00:00
abstract：:The epithelial Na/Pi cotransporter (NaPi-II) is instrumental in maintaining phosphate (Pi) homeostasis in vertebrates. Hormones and metabolic factors (PTH, Pi availability) that acutely influence renal Pi excretion have been demonstrated to target NaPi-II expression. Upon stimulation, newly synthesized transporter mol...
journal_title：Cell biochemistry and biophysics
pub_type： 杂志文章,评审
doi：10.1385/CBB:36:2-3:241
更新日期：2002-01-01 00:00:00
abstract：:This study is aimed to investigate the efficacy and underlying the mechanism of propofol in treatment of ischemia reperfusion (IR)-induced lung injury in rats, providing a novel insight of therapeutic strategy for IR-induced lung injury. 120 healthy SD rats were selected and randomly divided into sham operation group,...
journal_title：Cell biochemistry and biophysics
pub_type： 杂志文章
doi：10.1007/s12013-014-0088-0
更新日期：2014-12-01 00:00:00
abstract：:Intensive treatment for newborns with trisomy 13 is controversial because of their lethal prognosis. We report the better life prognosis of patients with trisomy 13 who received intensive treatment. At our hospital, we provided an intensive management to such patients including resuscitation and surgical procedures as...
journal_title：Cell biochemistry and biophysics
pub_type： 杂志文章
doi：10.1007/s12013-012-9355-0
更新日期：2012-07-01 00:00:00
abstract：:Calmodulin (CaM) binds to the FERM domain of 80 kDa erythrocyte protein 4.1R (R30) independently of Ca(2+) but, paradoxically, regulates R30 binding to transmembrane proteins in a Ca(2+)-dependent manner. We have previously mapped a Ca(2+)-independent CaM-binding site, pep11 (A(264)KKLWKVCVEHHTFFR), in 4.1R FERM domai...
journal_title：Cell biochemistry and biophysics
pub_type： 杂志文章
doi：10.1007/s12013-013-9758-6
更新日期：2014-05-01 00:00:00
abstract：:Despite the improvements in chemotherapy, about 60 % of acute myeloid leukemia (AML) remission patients still relapse. Leukemic stem cells (LSCs) are the main causes for the relapse and refractory. T cell immunoglobulin mucin-3 (TIM-3), a specific surface molecule expressed on LSCs in most types of AML, is a candidate...
journal_title：Cell biochemistry and biophysics
pub_type： 杂志文章
doi：10.1007/s12013-014-9900-0
更新日期：2014-09-01 00:00:00
abstract：:Curcumin is a yellow-orange dye widely used as a spice, food coloring and food preservative. It also exhibits a broad range of therapeutic effects against different disorders such as cancer, diabetes, or neurodegenerative diseases. As a compound insoluble in water curcumin accumulates in cell membranes and due to this...
journal_title：Cell biochemistry and biophysics
pub_type： 杂志文章
doi：10.1007/s12013-020-00906-5
更新日期：2020-06-01 00:00:00
abstract：:The simultaneous determination of adhesion and deformability parameters of erythrocytes was carried out through a microfluidic device, which uses an inverted optical microscope with new image acquisition and analysis technologies. Also, an update of the models describing erythrocyte adhesion and deformation was propos...
journal_title：Cell biochemistry and biophysics
pub_type： 杂志文章
doi：10.1007/s12013-020-00956-9
更新日期：2020-11-07 00:00:00
abstract：:A simple analytical approximation to an inhomogeneously-broadened dispersion signal is proposed and tested with resonance lines broadened by unresolved hyperfine structure. Spectral parameters may be rapidly and accurately extracted using a nonlinear least-squares fitting algorithm. Combining the new approximation to ...
journal_title：Cell biochemistry and biophysics
pub_type： 杂志文章
doi：10.1007/s12013-016-0760-7
更新日期：2017-06-01 00:00:00
