Abstract:
:A simple analytical approximation to an inhomogeneously-broadened dispersion signal is proposed and tested with resonance lines broadened by unresolved hyperfine structure. Spectral parameters may be rapidly and accurately extracted using a nonlinear least-squares fitting algorithm. Combining the new approximation to a dispersion signal with a well-known approximation to the absorption signal allows dispersion-absorption admixtures, a problem of growing importance, to be analyzed quickly and accurately. For pure dispersion signals, the maximum difference between the fit and the signal for unresolved lines is 1.1 % of the maximum intensity. For pure absorption, the difference is 0.33 % of the peak-to-peak intensity, and for admixtures up to 40 % dispersion (maximum intensity/peak-to-peak intensity), the difference is 0.7 %. The accuracy of the recovered spectral parameters depends on the degree of inhomogeneously-broadened and the percentage admixture, but they are generally about 1 % at most. A significant finding of the work is that the parameters pertinent to the dispersion or the absorption are insignificantly different when fitting isolated lines vs. fitting admixtures. Admixtures with added noise or an unsuspected extraneous line are investigated.
journal_name
Cell Biochem Biophysjournal_title
Cell biochemistry and biophysicsauthors
Bales BLdoi
10.1007/s12013-016-0760-7subject
Has Abstractpub_date
2017-06-01 00:00:00pages
171-184issue
2eissn
1085-9195issn
1559-0283pii
10.1007/s12013-016-0760-7journal_volume
75pub_type
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