Abstract:
:We recently established a high-speed, label-free, spectral imaging method based on stimulated Raman scattering (SRS). This method enables examination of cellular features within relatively short periods, thus enabling new imaging applications in pathology. Previously, we reported on label-free visualization of mouse tissue using SRS spectral microscopy combined with multivariate image analysis, but the feasibility of applying this approach to diseased tissues with diverse morphology and irregular chemical species has not been examined. We, therefore, assessed acetaminophen-induced liver injury to evaluate the potential use of Raman spectral microscopy for visualizing histopathologic specimens. Acetaminophen-overdosed mouse liver was prepared and the pathologic changes including centrilobular necrosis were confirmed. Multi-colored images were reconstructed through principal component analysis (PCA) of a multi-band SRS dataset, which provided rich information compared with a monochrome single-band SRS dataset. A wide view of the multi-colored principal component (PC) images showed the distribution of cellular constituents, which was similar to that observed by fat staining. In addition, different types of cells in liver parenchyma were also demonstrated. In conclusion, the combination of SRS spectral microscopy and PCA has the potential to reveal both the morphological and chemical features of specimens and therefore has potential utility in diagnostic pathology.
journal_name
Pathol Intjournal_title
Pathology internationalauthors
Satoh S,Otsuka Y,Ozeki Y,Itoh K,Hashiguchi A,Yamazaki K,Hashimoto H,Sakamoto Mdoi
10.1111/pin.12206subject
Has Abstractpub_date
2014-10-01 00:00:00pages
518-26issue
10eissn
1320-5463issn
1440-1827journal_volume
64pub_type
杂志文章abstract::A unique case of duodenal stromal tumor in a 51-year-old man is reported. The tumor histologically showed spindle cell proliferation and numerous eosinophilic globules. Most globules were composed of tangled 45 nm thick fibrils, which were ultrastructurally identical to 'skeinoid fibers'. The presence of glycogen gran...
journal_title:Pathology international
pub_type: 杂志文章
doi:10.1111/j.1440-1827.1997.tb04459.x
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journal_title:Pathology international
pub_type: 杂志文章
doi:10.1111/j.1440-1827.1995.tb03420.x
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abstract::Adenosquamous carcinoma (ASC) is a rare malignant neoplasm of the head and neck regions. We elucidated the relationship between ASC and MUC molecule expression. We selected 14 cases of ASC in the head and neck, and examined them immunohistohcmically. Seven cases of tongue, 3 cases of larynx and 4 cases of hypopharynx ...
journal_title:Pathology international
pub_type: 杂志文章
doi:10.1111/pin.12144
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journal_title:Pathology international
pub_type: 杂志文章
doi:10.1046/j.1440-1827.2002.01383.x
更新日期:2002-09-01 00:00:00
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journal_title:Pathology international
pub_type: 杂志文章,评审
doi:10.1046/j.1440-1827.2001.01288.x
更新日期:2001-11-01 00:00:00
abstract::Elderly patients with Epstein-Barr virus (EBV) infection are at increased risk for developing B-cell lymphoproliferative disorder (B-LPD) due to immunosenescence. Here, we describe a case of a 75-year-old man who developed an EBV-positive (EBV+) mucocutaneous ulcer (EBVMCU) in the gingiva with spontaneous regression. ...
journal_title:Pathology international
pub_type: 杂志文章
doi:10.1111/pin.12738
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journal_title:Pathology international
pub_type: 杂志文章
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更新日期:2007-03-01 00:00:00
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journal_title:Pathology international
pub_type: 杂志文章
doi:10.1046/j.1440-1827.2003.01551.x
更新日期:2003-11-01 00:00:00
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journal_title:Pathology international
pub_type: 杂志文章,多中心研究
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更新日期:2012-08-01 00:00:00
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journal_title:Pathology international
pub_type: 杂志文章
doi:10.1111/j.1440-1827.2012.02826.x
更新日期:2012-07-01 00:00:00
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journal_title:Pathology international
pub_type: 杂志文章
doi:10.1111/pin.12032
更新日期:2013-02-01 00:00:00
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journal_title:Pathology international
pub_type: 杂志文章
doi:10.1111/j.1440-1827.2008.02301.x
更新日期:2008-11-01 00:00:00
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journal_title:Pathology international
pub_type: 杂志文章
doi:10.1046/j.1440-1827.1999.00975.x
更新日期:1999-11-01 00:00:00
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journal_title:Pathology international
pub_type: 杂志文章
doi:10.1111/j.1440-1827.2012.02841.x
更新日期:2012-09-01 00:00:00
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journal_title:Pathology international
pub_type: 杂志文章
doi:10.1111/j.1440-1827.2012.02824.x
更新日期:2012-06-01 00:00:00
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journal_title:Pathology international
pub_type: 杂志文章
doi:10.1111/j.1440-1827.1998.tb03949.x
更新日期:1998-07-01 00:00:00
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journal_title:Pathology international
pub_type: 杂志文章
doi:10.1111/j.1440-1827.2004.01654.x
更新日期:2004-07-01 00:00:00
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journal_title:Pathology international
pub_type: 杂志文章
doi:10.1111/j.1440-1827.2007.02053.x
更新日期:2007-01-01 00:00:00
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journal_title:Pathology international
pub_type: 杂志文章
doi:10.1111/j.1440-1827.1996.tb03533.x
更新日期:1996-01-01 00:00:00
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journal_title:Pathology international
pub_type: 杂志文章
doi:10.1111/pin.12986
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journal_title:Pathology international
pub_type: 杂志文章,评审
doi:10.1111/j.1440-1827.1996.tb03639.x
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journal_title:Pathology international
pub_type: 杂志文章,评审
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更新日期:2001-07-01 00:00:00
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journal_title:Pathology international
pub_type: 杂志文章
doi:10.1046/j.1440-1827.2001.01293.x
更新日期:2001-11-01 00:00:00
abstract::The copper and iron status in the liver of non-tumor bearing Long-Evans Cinnamon (LEC) rats (average age 17 months) was investigated. A direct quantitation of loosely-bound copper and iron was also investigated by using a chelating agent, nitrilotriacetic acid (NTA-chelatable free copper and iron). Besides the total c...
journal_title:Pathology international
pub_type: 杂志文章
doi:10.1111/j.1440-1827.1997.tb04481.x
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journal_title:Pathology international
pub_type: 杂志文章
doi:10.1111/j.1440-1827.1996.tb03629.x
更新日期:1996-06-01 00:00:00
abstract::A 70-year-old man with metastatic liver tumors showed carcinoid syndrome with clinical symptoms of facial flushing, palpitation, dyspnea, and an itching sensation. Regurgitation of the tricuspid and aortic valves was observed by echocardiography. An elevated serum level of serotonin and a high urine excretion of serot...
journal_title:Pathology international
pub_type: 杂志文章
doi:10.1046/j.1440-1827.2000.01053.x
更新日期:2000-05-01 00:00:00
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journal_title:Pathology international
pub_type: 杂志文章
doi:10.1111/j.1440-1827.1997.tb04543.x
更新日期:1997-08-01 00:00:00
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journal_title:Pathology international
pub_type: 杂志文章
doi:10.1111/j.1440-1827.2006.02039.x
更新日期:2006-12-01 00:00:00
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journal_title:Pathology international
pub_type: 杂志文章
doi:10.1111/j.1440-1827.1994.tb02591.x
更新日期:1994-03-01 00:00:00
abstract::Twenty-eight moderately differentiated adenocarcinomas invading beyond the muscularis propria of the colorectum were subclassified as 13 moderate- and 15 poor-subtype tumors based on the histology at the deeply infiltrating sites. Moderately differentiated cancer cells were correlated with liver metastasis and p53 imm...
journal_title:Pathology international
pub_type: 杂志文章
doi:10.1111/j.1440-1827.1996.tb03598.x
更新日期:1996-03-01 00:00:00