Enzyme linked immunosorbent assay (ELISA) for beta-endorphin and its antibodies.

abstract：:Epinephrine (E) infusions raise blood pressure and there is an excess incidence of hypertension among males and blacks. However, reports of E levels by ethnicity, gender, and blood pressure status are inconsistent. Insensitive assays, variability in plasma E levels within individuals, and the small size of most studie...

journal_title：Life sciences

authors： González-Trápaga JL,Nelesen RA,Dimsdale JE,Mills PJ,Kennedy B,Parmer RJ,Ziegler MG

更新日期：2000-05-05 00:00:00

abstract：:Oxidative stress induced cell injury is reported to contribute to the pathogenesis of cerebral ischemia. Reactive oxygen species such as hydrogen peroxide (H2O2) and superoxide radical along with nitric oxide and peroxynitrite generated during ischemia-reperfusion injury, causes the overactivation of poly (ADP-ribose)...

journal_title：Life sciences

authors： Kaundal RK,Shah KK,Sharma SS

更新日期：2006-11-10 00:00:00

abstract：:It has widely been established that Cl- transport is defective in cystic fibrosis fibroblasts. In the present study, the effect of elevation of intracellular concentration of cyclic AMP and calcium on the efflux of Cl- from human fibroblasts has been investigated. Cyclic AMP analogs (8-bromo cAMP and dibutyryl cAMP) a...

journal_title：Life sciences

authors： Mastrocola T,Rugolo M

更新日期：1990-01-01 00:00:00

abstract：AIMS:GnRH-DFF40 (gonadotropin releasing hormone-DNA fragmentation factor 40) humanized recombinant immunotoxin serves as a prospective candidate for targeted therapy of malignancies with over-expressed gonadotropin releasing hormone receptor (GnRHR). In this study, we attempted to generate a GnRH-based chimeric protein...

journal_title：Life sciences

authors： Barazesh M,Mohammadi S,Jalili S,Kavousipour S,Faraji SN,Mokarram P,Pirhadi S

更新日期：2021-01-15 00:00:00

abstract：:Chronic social defeat stress (CSDS) is an ethologically relevant psychosocial stress animal model and has been widely used in depression studies. Ginsenoside Rg1 (Rg1) is the major active ingredients of ginseng with low toxicity and neuroprotective effects. The present study aims to investigate the antidepressant effe...

journal_title：Life sciences

authors： Jiang N,Lv J,Wang H,Huang H,Wang Q,Lu C,Zeng G,Liu XM

更新日期：2020-07-01 00:00:00

abstract：:Macrophages and osteoclasts derive from related cell lines. In osteopetrotic mutants the function of osteoclasts is greatly reduced compared to that in normal animals or children and macrophage function is variably affected depending upon the mutation. To further explore macrophage function in osteopetrosis we examine...

journal_title：Life sciences

authors： Popoff SN,Coderre JA,Marks SC Jr,Stock JL

更新日期：1989-01-01 00:00:00

abstract：:The influence of different, nitric oxide-containing ruthenium complexes on the evoked potentials recorded from the CA1 region of the mouse hippocampus in vitro has been investigated. Of the compounds tested, only trans-[(NO)(P(OEt)3)(NH3)4Ru](PF6)3 (1-2.5 mM) exerted a strong facilitatory action on the population spik...

journal_title：Life sciences

authors： Wieraszko A,Clarke MJ,Lang DR,Lopes LG,Franco DW

更新日期：2001-02-16 00:00:00

abstract：:Despite the recent scientific advances made in cancer diagnostics and therapeutics, cancer still remains the second leading cause of death worldwide. Thus, there is a need to identify new potential biomarkers/molecular targets to improve the diagnosis and treatment of cancer patients. In this regard, long non-coding R...

journal_title：Life sciences

authors： Sharma U,Barwal TS,Malhotra A,Pant N,Vivek,Dey D,Gautam A,Tuli HS,Vasquez KM,Jain A

更新日期：2020-09-15 00:00:00

abstract：:We examined the effects of various oleanolic acid oligoglycosides obtained from traditional herbs on ethanol- or indomethacin-induced gastric mucosal lesions in rats and on gastric secretion in pylorus-ligated rats. Test samples were given orally to fasted rats 1 h before absolute ethanol (1.5 ml/rat, p.o.) or indomet...

journal_title：Life sciences

authors： Matsuda H,Li Y,Murakami T,Yamahara J,Yoshikawa M

更新日期：1998-01-01 00:00:00

abstract：:The activation of cholinergic pathways by nicotine elicits various physiological and pharmacological effects in mammals. For example, the stimulation of nicotinic acetylcholine receptors (nAChRs) leads to an antinociceptive effect. However, it remains to be elucidated which subtypes of nAChR are involved in the antino...

journal_title：Life sciences

authors： Abdin MJ,Morioka N,Morita K,Kitayama T,Kitayama S,Nakashima T,Dohi T

更新日期：2006-12-03 00:00:00

abstract：:Resting pituitary levels of beta-endorphin-(beta-EP-IR), ACTH-(ACTH-IR), and alpha-MSH-(alpha-MSH-IR)-like immunoreactive material were found to differ among 16 inbred mouse strains. Hormone levels correlated genetically with severity of withdrawal from ethanol, which also differed among the strains. Ethanol dependenc...

journal_title：Life sciences

authors： Crabbe JC,Keith LD,Kosobud A,Stack J

更新日期：1983-11-07 00:00:00

abstract：:Heat shock protein 70 (Hsp70) is capable of protecting cells, tissues, organs, and animals from a subsequent, normally lethal heating, as well as from numerous disease states. Therefore, it would be of great therapeutic benefit to discover compounds that are clinically safe yet able to induce Hsp70 in patients. Carben...

journal_title：Life sciences

authors： Nagayama S,Jono H,Suzaki H,Sakai K,Tsuruya E,Yamatsu I,Isohama Y,Miyata T,Kai H

更新日期：2001-11-02 00:00:00

abstract：:The effects of capsaicin on cellular growth and intracellular calcium mobilization were examined in human cervical carcinoma derivation, HeLa cells. Capsaicin inhibited cellular growth and increased intracellular calcium level in HeLa cells. This capsaicin-induced intracellular calcium concentration rise was blocked b...

journal_title：Life sciences

authors： Takahata K,Chen X,Monobe K,Tada M

更新日期：1999-01-01 00:00:00

abstract：:Although several reports imply that anticoagulants and preservatives contain zinc, the quantity of zinc in heparin, if any, has not been documented. Zinc concentration was determined by flame atomic absorption spectroscopy in varying dilutions of multiple commercially obtained samples of purified sodium heparin N = 15...

journal_title：Life sciences

authors： Gervin CA,Gervin AS,Nichols W,Corrigan JJ Jr

更新日期：1983-12-26 00:00:00

abstract：:Studies were performed to compare in vitro and in vivo effects of morphine on the phagocytic function of murine peritoneal macrophages. Macrophage monolayers were incubated with Candida albicans for 30 min in the absence of autologous serum. Morphine added in vitro was found to decrease both the phagocytic activity (p...

journal_title：Life sciences

authors： Rojavin M,Szabo I,Bussiere JL,Rogers TJ,Adler MW,Eisenstein TK

更新日期：1993-01-01 00:00:00

abstract：:Hypoxia-induced neuronal damage and glutamate release were investigated in a N(2)- or in xenon-atmosphere for embryonic rat cortical neurons; cellular damage and glutamate over-release were observed in N(2)-treated cells whereas xenon protected the cells from the hypoxic insult. The protective effect of xenon was stro...

journal_title：Life sciences

authors： Petzelt C,Blom P,Schmehl W,Müller J,Kox WJ

更新日期：2003-03-14 00:00:00

abstract：:Several cryopreservation methods for precision-cut rat liver slices (PCLS) have been proposed, allowing a short-term (a few hours) maintainance of viability and functionality upon thawing. The aim of the present study was to test the metabolic capacity of PCLS cryopreserved by an ultrarapid method. The biotransformati...

journal_title：Life sciences

authors： Vanhulle VP,Martiat GA,Verbeeck RK,Horsmans Y,Calderon PB,Eeckhoudt SL,Taper HS,Delzenne N

更新日期：2001-04-13 00:00:00

abstract：:Different receptors mediating the release of endothelium-derived nitric oxide (EDNO) have been identified at endothelial level. In the present study we aimed to characterise, on rabbit aorta by means of pharmacological tools, the generation of EDNO by receptors located on endothelial cell membrane (M3, P2u, P2y) and b...

journal_title：Life sciences

authors： Chinellato A,Froldi G,Caparrotta L,Ragazzi E

更新日期：1998-01-01 00:00:00

abstract：AIMS:Epigenetic and genetic alterations are crucial events in the onset and progression of human cancers including colorectal cancer (CRC). This work aims to probe the relevance of lysine demethylase 5B (KDM5B) to the progression of CRC and the possible molecules involved. MATERIALS AND METHODS:KDM5B expression in CRC...

journal_title：Life sciences

authors： Yan G,Li S,Yue M,Li C,Kang Z

更新日期：2021-01-01 00:00:00

abstract：AIMS:Numerous studies confirmed the involvement of extracellular vesicles in cancer development and progression. The present study was designed to investigate the glycan composition of ectosomes derived by human cutaneous melanoma (CM) cell lines with the use of lectins. MAIN METHODS:Ectosomes released by primary (WM1...

journal_title：Life sciences

authors： Surman M,Hoja-Łukowicz D,Szwed S,Drożdż A,Stępień E,Przybyło M

更新日期：2018-08-15 00:00:00

abstract：:Protein kinase C (PKC)-dependent phosphorylation of endogenous substrates was measured in electrically permeabilised rat islets of Langerhans. The PKC-activating phorbol ester, 4 beta-phorbol myristate acetate (PMA), caused a slow but prolonged increase in insulin secretion from permeabilised islets, which was accompa...

journal_title：Life sciences

authors： Jones PM,Persaud SJ,Howell SL

更新日期：1992-01-01 00:00:00

abstract：:Three distinct peptidyldipeptidases (exopeptidases releasing carboxyl terminal dipeptide residues) can be solubilized from nerve terminal membrane fractions from whole rat brain or striatum, and separated by ion exchange chromatography. Brain angiotensin-converting enzyme (PDP-1) cleaves Hip-His-Leu, but not 80 nM [3H...

journal_title：Life sciences

authors： Cushman DW,Gordon EM,Wang FL,Cheung HS,Tung R,Delaney NG

更新日期：1983-01-01 00:00:00

abstract：:A number of potential functions of thioredoxin have been proposed in literature, including a role for DNA replication. The aim of our study was to investigate the effects of thioredoxin from Streptomyces aureofaciens (Trx S.a.) on plasmid DNA. Trx S.a. was incubated with plasmid forms and the incubation product(s) cha...

journal_title：Life sciences

authors： Golubnitchaya-Labudova O,Horecka T,Kapalla M,Perecko D,Kutejova E,Lubec G

更新日期：1998-01-01 00:00:00

abstract：:In order to investigate the action of ethanol on the anterior pituitary gland, the effect of ethanol on prolactin secretion in vitro was studied. Ethanol significantly increased the in vitro incorporation of 3H-leucine into both prolactin contained within the pituitary gland and that released into the medium. The enha...

journal_title：Life sciences

authors： Seilicovich A,Duvilanski BH,Debeljuk L,Díaz MC,Muñoz Maines V,Rettori V

更新日期：1984-11-05 00:00:00

abstract：:The effect of thapsigargin, a specific inhibitor of intracellular Ca(2+)-ATPases, on endothelium-dependent relaxation was studied in the guinea pig thoracic aorta. Thapsigargin (1 nM-1 microM) produced a concentration-dependent relaxation of aortic strips precontracted by phenylephrine (0.1 microM) in the presence and...

journal_title：Life sciences

authors： Matsuyama S,Shuntoh H,Katayama S,Tanaka C

更新日期：1993-01-01 00:00:00

abstract：:Inducible nitric oxide synthase (iNOS) and cyclooxygenase (COX)-2 are expressed in vascular smooth muscle cells stimulated with interleukin-1beta (IL-1beta), resulting in the production of nitric oxide (NO) and prostaglandins (PGs) such as PGI2. The iNOS and COX-2 proteins and their mRNA expressions in cultured vascul...

journal_title：Life sciences

authors： Hirafuji M,Tsunoda M,Machida T,Hamaue N,Endo T,Miyamoto A,Minami M

更新日期：2002-01-11 00:00:00

abstract：:Uptake of serotonin and 3H-imipramine binding in platelets of depressed patients were investigated simultaneously with changes in clinical state. Both Vmax for serotonin uptake and Bmax for 3H-imipramine binding were significantly lower in unmedicated depressed patients with respect to normal subjects. Successful trea...

journal_title：Life sciences

authors： Suranyi-Cadotte BE,Quirion R,Nair NP,Lafaille F,Schwartz G

更新日期：1985-02-25 00:00:00

abstract：:The compound (+)-MR200 [(+)-methyl (1R,2S)-2-{[4-(4-chlorophenyl)-4-hydroxypiperidin-1-yl]methyl}-1-phenylcyclopropanecarboxylate] is a sigma ligand with increased affinity and selectivity compared to the structurally related ligand haloperidol. From the results of a previous study on the modulation of a systemically ...

journal_title：Life sciences

authors： Marrazzo A,Parenti C,Scavo V,Ronsisvalle S,Scoto GM,Ronsisvalle G

更新日期：2006-04-18 00:00:00

abstract：:The relationship between prolactin (PRL) secretion and the neurointermediate lobe (NIL) of the pituitary gland was investigated. Plasma PRL concentrations in rats bearing anterior pituitaries autografted with or without the NIL to the renal capsule were elevated to equal extents at 1 through 6 weeks after surgery (p g...

journal_title：Life sciences

authors： Fagin KD,Neill JD

更新日期：1982-03-29 00:00:00

abstract：:The ability of metformin (MF) to acutely relax phenylephrine (PE)-induced contraction in the isolated rat tail artery is reported to be accompanied by repolarization of the arterial smooth muscle cell (SMC) membranes. These membranes contain potassium (K) channels which if opened could mediate such repolarization and ...

journal_title：Life sciences

authors： Peuler JD,Lee JM,Smith JM

更新日期：1999-10-29 00:00:00