Abstract：
:It has widely been established that Cl- transport is defective in cystic fibrosis fibroblasts. In the present study, the effect of elevation of intracellular concentration of cyclic AMP and calcium on the efflux of Cl- from human fibroblasts has been investigated. Cyclic AMP analogs (8-bromo cAMP and dibutyryl cAMP) and a beta agonist (isoproterenol) induced only a weak stimulation (5-10%) of Cl- efflux. Conversely, elevation of cytoplasmic calcium concentration produced by addition of the Ca2+ ionophore A23187 in the efflux medium, did not affect Cl- efflux. Our data indicate that the response of Cl- efflux to elevation of cAMP and calcium is similar in normal and cystic fibrosis fibroblasts. Exposure to hypotonic medium induced a significant stimulation of Cl- efflux in fibroblasts from both normal and cystic fibrosis individuals. Substitution of Cl- in the medium by gluconate and the subsequent addition of furosemide did not inhibit the effect of hypotonicity, indicating the involvement of a conductive pathway for Cl- transport, which was insensitive to oligomicin C.
journal_name
Life Scijournal_title
Life sciencesauthors
Mastrocola T,Rugolo Mdoi
10.1016/0024-3205(90)90381-zsubject
Has Abstractpub_date
1990-01-01 00:00:00pages
1661-9issue
23eissn
0024-3205issn
1879-0631pii
0024-3205(90)90381-Zjournal_volume
46pub_type
杂志文章相关文献
LIFE SCIENCES文献大全abstract：:Gluten exorphin B5 (GE-B5) is a food-derived opioid peptide identified in digests of wheat gluten. We have recently shown that GE-B5 stimulates prolactin (PRL) secretion in rats; this effect is abolished by preadministration of the opioid receptor antagonist naloxone. However, since the structure of naloxone allows it...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2004.09.023
更新日期：2005-02-25 00:00:00
abstract：:Amyloid beta protein (Abeta) alters signal transduction systems, including increases in the cytosolic free calcium ([Ca2+]i) response which have pathophysiological significance in Alzheimer's disease (AD). The purposes of this study were to elucidate the mechanism involved in Abeta's effect on the Ca2+ signal and to e...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(00)00470-7
更新日期：2000-01-01 00:00:00
abstract：:The extended infection with Helicobacter pylori (H. pylori), one of the most frequent infectious agents in humans, may cause gastritis, peptic ulcers, gastric mucosa-associated lymphoid tissue (MALT) lymphoma, and gastric cancer. During H. pylori infection, different kinds of inflammatory cells such as dendritic cells...
journal_title：Life sciences
pub_type： 杂志文章,评审
doi：10.1016/j.lfs.2019.116688
更新日期：2019-08-15 00:00:00
abstract：AIMS:The aim of this study is to determine whether Nkx2.5 transfection of transplanted bone marrow mesenchymal stem cells (MSCs) improves the efficacy of treatment of adriamycin-induced heart failure in a rat model. MAIN METHODS:Nkx2.5 was transfected in MSCs by lentiviral vector transduction. The expressions of Nkx2....
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2017.06.014
更新日期：2017-08-01 00:00:00
abstract：:The elevated plus-maze test has been widely used for screening of anxiolytic drugs and for exploring neurobiological bases of anxiety. In this study, we validated a new automated system that enables to record exploratory behavior in the elevated plus-maze test. This system, called cyberplus, consisted of ten pairs of ...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(02)01476-5
更新日期：2002-03-01 00:00:00
abstract：:The concentrations of the trace amines, m-tyramine, p-tyramine, phenylethylamine and tryptamine, were measured in the striatum of the brain and in the kidney of adult rats treated with alpha-monofluoromethyldopa (MFMD), an inhibitor of aromatic amino acid decarboxylase. While MFMD decreased the levels of all four amin...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(87)90371-7
更新日期：1987-02-09 00:00:00
abstract：:The accumulation of lysophosphoglycerides has been implicated as an important biochemical factor for cardiac arrhythmias. Recently, we demonstrated that lysophosphatidylcholine caused cardiac arrhythmias in the isolated hamster heart. In this study, the arrhythmogenic nature of various lysophosphoglycerides with respe...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(83)90806-8
更新日期：1983-03-21 00:00:00
abstract：:The intestinal mucosa of infant rats was found to produce ketones when incubated in Krebs-Ringer-Bicarbonate solution. No production was found in weaned rats. Ketogenesis could be inhibited by D-carnitine or tetradecylglycidic acid (TDGA) an inhibitor of long-chain acylcarnitine transferase, suggesting that ketone pro...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(87)90718-1
更新日期：1987-09-21 00:00:00
abstract：:The role of the autonomic nervous system in the modulation of the immune response to emotional stimuli, was established in rats subjected to the passive avoidance test. An increase in splenic primary antibody response directed against SRBC was found after exposure of rats to the passive avoidance apparatus (novelty). ...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(90)90085-6
更新日期：1990-01-01 00:00:00
abstract：:Oxidative stress (free radical generation exceeds antioxidant defense) occurs in many diseases and after exposure to certain chemicals. We automated assays estimating the antioxidant status of serum and lipid hydroperoxide concentrations using the Syva-30R automated analyzer. In this assay, the antioxidant status of s...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(97)00401-3
更新日期：1997-01-01 00:00:00
abstract：:Intestinal mucosal capsaicin-sensitive afferent nerves mediate, in part, the protective mesenteric hyperemia after intraduodenal acidification. Mechanisms associated the sensory neuropeptides, e.g. calcitonin gene-related peptide (CGRP), substance P, and ruthenium red-sensitive cation channels contribute to acid-induc...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(02)02106-9
更新日期：2002-10-18 00:00:00
abstract：:Increased levels of particulate matter (PM) air pollutants in East Asia have resulted in detrimental health impacts increasing morbidity and mortality. Epidemiological studies suggest a possible relation between the cutaneous exposure of PM and increased oxidative stress and inflammation which lead to skin lesions. Th...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2019.116714
更新日期：2019-09-15 00:00:00
abstract：:Tifluadom, although structurally a 1,4 benzodiazepine, has no affinity for the 3H-flunitrazepam binding site, but is a potent displacer of 3H-bremazocine from its opioid binding site. Tifluadom is characterised as an opiate kappa-receptor agonist in vitro and in vivo with potent analgesic activity in animals and no de...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(82)90346-0
更新日期：1982-09-20 00:00:00
abstract：:Lymphocyte apoptosis occurs in response to stressors such as thermal injury, trauma, sepsis, and surgery. This study evaluated the effect of a single bout of physical exercise stress on the induction of apoptosis in murine thymocytes and splenic lymphocytes. Female C57BL/6 mice, treadmill exercised at a submaximal int...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(98)00551-7
更新日期：1999-01-01 00:00:00
abstract：AIMS:The effects of chronic administration of Ferutinin (phytoestrogen found in the plants of genus Ferula), compared with those elicited by estradiol benzoate, were evaluated, following ovariectomy, on the uterus of ovariectomized rats as regard weight, size, structure and histomorphometry. MAIN METHODS:The experimen...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2011.11.001
更新日期：2012-01-16 00:00:00
abstract：AIMS:Chloropropanol is a contaminant produced during food processing, and 1,3-dichloro-2-propanol (1,3-DCP) is one of the most-studied and most common chloropropanol-related food contaminants. Epigallocatechin-3-gallate (EGCG) is the most abundant ester catechin in tea polyphenols. We studied the potential therapeutic ...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2018.08.007
更新日期：2018-09-15 00:00:00
abstract：OBJECTIVE:We aimed to explore the expression level and biological function of miR-145-5p in preeclampsia (PE). METHODS:The differentially expressed miRNA/mRNA between normal placentas and PE placentas were screened using the GSE84260 and GSE73374 datasets from the Gene Expression Omnibus Database. The expression of mi...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2019.117008
更新日期：2019-12-15 00:00:00
abstract：AIMS:To study the underlying mechanisms of sulforaphane, a natural histone deacetylase (HDAC) inhibitor, in inhibiting triple negative breast cancer cells growth and the therapeutic effects of combination of sulforaphane and doxorubicin in TNBC treatment. MATERIALS AND METHODS:The antineoplastic activity of sulforapha...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2018.10.034
更新日期：2018-11-15 00:00:00
abstract：:We contrast the effects of three intermediate electron acceptors (IEAs) on the highly quantitative ESTA bioassay for human growth hormone. This is a microculture tetrazolium assay based upon the in vitro reduction of the tetrazolium salt MTT, by Nb2 cells which have been activated with hGH. Each of the IEAs influenced...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(96)00512-7
更新日期：1996-01-01 00:00:00
abstract：:The α chain of interleukin 3 receptor (IL-3Rα or CD123), together with the common β (βc) subunit, forms a high-affinity IL-3R with biological function. In recent years, emerging research has found that CD123 is highly expressed on the surface of various cells (e.g., leukemia stem cells, LSCs), and it is associated wit...
journal_title：Life sciences
pub_type： 杂志文章,评审
doi：10.1016/j.lfs.2014.10.013
更新日期：2015-02-01 00:00:00
abstract：:Rats with experimental allergic encephalomyelitis (EAE) induced with myelin or spinal cord show decreases in the content of sulphatides and cerebrosides and increases in the level of esterified cholesterol in the CNS. In this work it is shown that brain sulphatide changes can be obtained by injection of mixtures conta...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(82)90600-2
更新日期：1982-03-08 00:00:00
abstract：:Doxorubicin and daunorubicin, the anthracycline antitumor agents, were evaluated for their in vitro and in vivo effect on phosphodiesterase (PDE) activity in mouse tissues. Doxorubicin at a concentration of 10(-4)M inhibited cardiac c-AMP (adenosine 3',5', monophosphate) PDE activity 50% of the control whereas in lung...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(85)90641-1
更新日期：1985-02-11 00:00:00
abstract：:This paper gives a short account of the mechanisms regulating choline production. Although choline is absorbed from the GI tract and biosynthesized in the liver, its subsequent metabolism to choline esters and phospholipids seems to dominate in importance as to its regulatory role in maintaining a constant source of f...
journal_title：Life sciences
pub_type： 杂志文章,评审
doi：10.1016/0024-3205(96)00181-6
更新日期：1996-01-01 00:00:00
abstract：:The nephrotic syndrome is a renal disease characterized by proteinuria, hypoproteinemia, edema and hyperlipidemia. It has been reported that female nephrotic rats are characterized by loss of the oestrus cycle, follicle atresia, low gonadotropin and steroid concentrations; particularly, undetectable estradiol levels. ...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2006.02.023
更新日期：2006-07-10 00:00:00
abstract：:Physical exercise is known to increase oxygen consumption to compensate for enhanced ATP consumption and thus to induce oxidative stress in tissues. Our previous data indicate that training significantly increased the hydroxyl radical level in rat tissues after physical exercise, but reduced the basal level in aged ra...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(98)00468-8
更新日期：1998-01-01 00:00:00
abstract：:Phosphatidylinositol (PI) specific phospholipase C treatment of rabbit platelets caused 95% release of acetylcholinesterase in the supernatant and 4 to 6% hydrolysis of membrane PI in 2 min. Under these conditions there was no cell lysis as monitored by lack of lactate dehydrogenase activity in the medium. The phospho...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(86)90590-4
更新日期：1986-02-24 00:00:00
abstract：:Changes in the activities of protein kinase A (cAMP-dependent), protein kinase C (Ca2+/phospholipid-dependent), and protein kinase M (Ca2+/calmodulin-dependent) in dog heart and liver were studied 4 hr following endotoxin injection. Protein kinases A and C were extracted and partially purified by DEAE-cellulose chroma...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(94)00838-8
更新日期：1994-01-01 00:00:00
abstract：:A mechanism of antidepressant activity of beta-amyrin palmitate was studied using the forced swimming method in mice. Beta-amyrin palmitate (10 mg/kg) reduced the increase in the duration of immobility induced by tetrabenazine (100 and 200 mg/kg), but showed no effect on that in mice treated with alpha-methyl-para-tyr...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(93)90220-w
更新日期：1993-01-01 00:00:00
abstract：AIMS:The long non-coding RNA (lncRNA) was reported to be involved in the progress of various cancers, however, its effect in colorectal cancer (CRC) remains unknown. The goal of the present study is to investigate the function role of lncRNA PCAT-1 in colorectal cancer. MAIN METHODS:The expression of lncRNA PCAT-1 in ...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2017.08.024
更新日期：2017-11-01 00:00:00
abstract：:The effects of the mixed class I and II mGLUR agonist (+) 1S,3R-trans-amino-cyclopentane-1,3-dicarboxylic acid (ACPD) and antagonists (+) alpha-methyl-4-carboxyphenylglycine (MCPG) and L-2-amino-3-phosphonopropionic acid (L-AP3) on the basal neuronal excitability and on the expression of in vitro epileptiform activity...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(97)00941-7
更新日期：1997-01-01 00:00:00
